Modular Multi-Stage Axial Compressor Design

Increasingly, companies are becoming more interested in reducing cost. Recent studies indicate that up to 80% of the Lifecycle costs (LCC) has been embedded in the engine\u27s DNA at the end of the development and design phase. One concept to aid in the cost reduction is the modular design of expensive and development-intensive components, such as multi-stage axial compressors. It is the objective of this approach to utilize a core module in all the compressors, thus maximizing commonality and minimizing all relevant development, design, manufacture, procurement, and service costs; these reductions in cost are projected to increase affordability by five-fold. This thesis introduces the modular concept with a multi-stage high-pressure compressor design carried out to through flow analysis. The compressor is consequently divided up into five modules, and a modular upgrade is then developed for a different application using the same core. Discussion is presented as to advantages and potential limitations

Mutagênese insercional da estirpe PAL5 de Gluconacetobacter diazotrophicus por transposição in vitro.

O presente trabalho mostra a utilidade da técnica de mutagênese insercional para a estirpe PAL5 de G. diazotrophicus, usando o transposon comercial EZ::Tn5 transposon insertion kit (Epicentre, número de catálogo EZI982K). Para avaliar a utilidade desta estratégia, a técnica foi aplicada ao gene GdluxI (GenBank YP 001603070), envolvido em monitoramento populacional ou quorum sensing, e que foi identificado no genoma da estirpe PAL5 (ROUWS, 2008). Esta técnica de mutagênese insercional não se restringe a este gene e pode ser aplicada a outros genes da estirpe PAL5 de G. diazotrophicus.bitstream/item/42765/1/COT116-09.pd

MILP approach for then design of vertical vapor-liquid separation vessels-comparison with heuristics

In this article we compare results from different heuristics approaches for the design of VLE separation vessels. In addition, we present an MILP approach that embeds the aforementioned heuristics and considers the discrete nature of the geometric variables. We show that different heuristics render different results and, while results from heuristics and MILP often coincide, significant departures occur.Fil: Fischer, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo y Diseño. Universidad Tecnológica Nacional. Facultad Regional Santa Fe. Instituto de Desarrollo y Diseño; ArgentinaFil: Costa, André Hemerly L.. Universidade do Estado de Rio do Janeiro; BrasilFil: Bagajewicz, Miguel J.. Oklahoma State University; Estados Unido

Influência do tratamento de superfície no desempenho anticorrosivo de um esquema de pintura

Avaliou-se, quanto à proteção anticorrosiva, três tratamentos de superfície, a saber, jateamento abrasivo seco e remoção de contaminantes por ferramentas mecânicas/manuais (segundo metodologias das normas ISO 8501-1 e SSPC SP11). Corpos-de-prova, com grau C de intemperismo inicial, foram submetidos a estes métodos de limpeza de superfície e, após aplicação de um esquema de pintura, foram avaliados em um ensaio acelerado de corrosão e em um ensaio de imersão em solução de NaCl 10-2 M. A eficiência do revestimento, quanto à proteção anticorrosiva, foi acompanhada por espectroscopia de impedância eletroquímica (EIS) e ensaios de aderência por tração, bem como pela análise dos resultados de avaliação do revestimento em relação ao empolamento. Concluiu-se que o jateamento abrasivo seco confere maior proteção anticorrosiva ao substrato, sendo seguido pelo tratamento com ferramentas mecânicas e/ou manuais segundo norma ISO 8501-1. O método referente à norma SSPC SP11 foi o menos eficaz

MODULAR MULTI-STAGE AXIAL COMPRESSOR DESIGN: A CONCEPTUAL STUDY WITH AN EXAMPLE

ABSTRACT Increasingly, companies are becoming more interested in reducing cost. Initially, cost reduction efforts were focused on the manufacturing and service side since the majority of the budget was consumed during that phase. However, recent studies indicate that up to 80% of the life cycle costs (LCC) has been embedded in the engine's DNA at the end of the development and design phase. This fact is motivating many companies to consider cost cutting initiatives much earlier in the development cycle than before. One concept to aid in such cost reduction is the modular design of expensive and development-intensive components, such as multi-stage axial compressors. With sufficient planning, any potentially negative impact on performance can be addressed and almost entirely eliminated. Conceptually, the compressor is divided into five (5) functional modules. In the successful modular design, the core module size is maximized, while all other modules are held to a minimum. It is the objective of this approach to utilize the core module in all the compressors, thus, maximizing commonality and minimizing all relevant development, design, manufacture, procurement, and service costs. This paper introduces the modular concept with an example; a multi-stage high pressure compressor (HPC) design is carried out to the preliminary meanline phase. The compressor is consequently divided up into its five (5) modules, and a modular upgrade is then developed for a different application using the same core. Discussion is presented as to the advantages and potential limitations

AIP1 is a novel Agenet/Tudor domain protein from Arabidopsis that interacts with regulators of DNA replication, transcription and chromatin remodeling

Background: DNA replication and transcription are dynamic processes regulating plant development that are dependent on the chromatin accessibility. Proteins belonging to the Agenet/Tudor domain family are known as histone modification "readers" and classified as chromatin remodeling proteins. Histone modifications and chromatin remodeling have profound effects on gene expression as well as on DNA replication, but how these processes are integrated has not been completely elucidated. It is clear that members of the Agenet/Tudor family are important regulators of development playing roles not well known in plants. Methods: Bioinformatics and phylogenetic analyses of the Agenet/Tudor Family domain in the plant kingdom were carried out with sequences from available complete genomes databases. 3D structure predictions of Agenet/Tudor domains were calculated by I-TASSER server. Protein interactions were tested in two-hybrid, GST pulldown, semi-in vivo pulldown and Tandem Affinity Purification assays. Gene function was studied in a T-DNA insertion GABI-line. Results: In the present work we analyzed the family of Agenet/Tudor domain proteins in the plant kingdom and we mapped the organization of this family throughout plant evolution. Furthermore, we characterized a member from Arabidopsis thaliana named AIP1 that harbors Agenet/Tudor and DUF724 domains. AIP1 interacts with ABAP1, a plant regulator of DNA replication licensing and gene transcription, with a plant histone modification "reader" (LHP1) and with non modified histones. AIP1 is expressed in reproductive tissues and its down-regulation delays flower development timing. Also, expression of ABAP1 and LHP1 target genes were repressed in flower buds of plants with reduced levels of AIP1. Conclusions: AIP1 is a novel Agenet/Tudor domain protein in plants that could act as a link between DNA replication, transcription and chromatin remodeling during flower development

Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways

Sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. Other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. Here we aim to study the effects of the association between the diazotroph Gluconacetobacter diazotrophicus PAL5 and sugarcane cv. SP70-1143 during water depletion by characterizing differential transcriptome profiles of sugarcane. RNA-seq libraries were generated from roots and shoots of sugarcane plants free of endophytes that were inoculated with G. diazotrophicus and subjected to water depletion for 3 days. A sugarcane reference transcriptome was constructed and used for the identification of differentially expressed transcripts. The differential profile of non-inoculated SP70-1143 suggests that it responds to water deficit stress by the activation of drought-responsive markers and hormone pathways, as ABA and Ethylene. qRT-PCR revealed that root samples had higher levels of G. diazotrophicus 3 days after water deficit, compared to roots of inoculated plants watered normally. With prolonged drought only inoculated plants survived, indicating that SP70-1143 plants colonized with G. diazotrophicus become more tolerant to drought stress than non-inoculated plants. Strengthening this hypothesis, several gene expression responses to drought were inactivated or regulated in an opposite manner, especially in roots, when plants were colonized by the bacteria. The data suggests that colonized roots would not be suffering from stress in the same way as non-inoculated plants. On the other hand, shoots specifically activate ABA-dependent signaling genes, which could act as key elements in the drought resistance conferred by G. diazotrophicus to SP70-1143. This work reports for the first time the involvement of G. diazotrophicus in the promotion of drought-tolerance to sugarcane cv. SP70-1143, and it describes the initial molecular events that may trigger the increased drought tolerance in the host plant

Método para alteração do desenvolvimento e formação de órgãos de uma planta

Em 15/03/2016: Notificação de devolução do pedido por não se enquadrar no Art. 229-C da LPI. Em 24/11/2015: Manutenção do arquivamento. Em 14/07/2015: Arquivamento – Art. 86 da LPI. Arquivado o pedido por falta de pagamento de anuidade, por pagamento de anuidade fora do prazo ou por não cumprimento de exigência de complementação de pagamento de anuidade.Não concedidaA presente invenção se refere a um método para alteração do desenvolvimento e formação de órgãos de uma planta, em particular aceleração da taxa de desenvolvimento, aumento do tamanho e número de órgãos e promoção de floração precoce, através de expressão aumentada ou diminuída de um ácido nucleico de cdc27a e/ou atividade e/ou níveis aumentados ou diminuídos, em uma planta, de uma proteína CDC27A. A invenção também se refere a plantas transgênicas tendo desenvolvimento alterado, plantas as quais têm expressão aumentada ou diminuída de um ácido nucleico que codifica uma proteína CDC27A