Разработка плана тактических действий по тушению пожаров в зданиях повышенной этажности (на примере жилого дома с административными помещениями, этажность 25)

Анализ пожаров в городе Новокузнецке показывает, что около 70% пожаров происходит в жилом секторе. При этом в жилье гибнет при пожарах около 80% людей по последним статистическим данным. Объектом исследования является жилой дом, расположенный в городе Новокузнецке, центральный район. Цель дипломного проекта – разработать тактические действия по тушению пожаров в зданиях повышенной этажности на примере вышеупомянутого объекта исследования. В процессе исследования был проведен аналитический обзор научных трудов и информационных статей по вопросам обеспечения пожарной безопасности в зданиях промышленной этажности, представлена характеристика исследуемого объекта и факторы, влияющие на развитие пожара в высотных зданиях.Analysis of fires in the city of Novokuznetsk shows that about 70% of fires occur in the residential sector. While the housing dies in fires, about 80% of people according to recent statistics. The object of study is a residential building located in the city of Novokuznetsk Central district. The aim of the project is to develop tactical actions on a touch-NIJ fires in high-rise buildings on the example of the above-mentioned object of study. In the process of the study was an analytical review of scientific papers and news articles on issues of fire safety in buildings, industrial heights, are the characteristic of the object studied and the factors affecting the development of fire in high-rise buildings

Mycobacteria Attenuate Nociceptive Responses by Formyl Peptide Receptor Triggered Opioid Peptide Release from Neutrophils

In inflammation, pain is regulated by a balance of pro- and analgesic mediators. Analgesic mediators include opioid peptides which are secreted by neutrophils at the site of inflammation, leading to activation of opioid receptors on peripheral sensory neurons. In humans, local opioids and opioid peptides significantly downregulate postoperative as well as arthritic pain. In rats, inflammatory pain is induced by intraplantar injection of heat inactivated Mycobacterium butyricum, a component of complete Freund's adjuvant. We hypothesized that mycobacterially derived formyl peptide receptor (FPR) and/or toll like receptor (TLR) agonists could activate neutrophils, leading to opioid peptide release and inhibition of inflammatory pain. In complete Freund's adjuvant-induced inflammation, thermal and mechanical nociceptive thresholds of the paw were quantified (Hargreaves and Randall-Selitto methods, respectively). Withdrawal time to heat was decreased following systemic neutrophil depletion as well as local injection of opioid receptor antagonists or anti-opioid peptide (i.e. Met-enkephalin, β-endorphin) antibodies indicating an increase in pain. In vitro, opioid peptide release from human and rat neutrophils was measured by radioimmunoassay. Met-enkephalin release was triggered by Mycobacterium butyricum and formyl peptides but not by TLR-2 or TLR-4 agonists. Mycobacterium butyricum induced a rise in intracellular calcium as determined by FURA loading and calcium imaging. Opioid peptide release was blocked by intracellular calcium chelation as well as phosphoinositol-3-kinase inhibition. The FPR antagonists Boc-FLFLF and cyclosporine H reduced opioid peptide release in vitro and increased inflammatory pain in vivo while TLR 2/4 did not appear to be involved. In summary, mycobacteria activate FPR on neutrophils, resulting in tonic secretion of opioid peptides from neutrophils and in a decrease in inflammatory pain. Future therapeutic strategies may aim at selective FPR agonists to boost endogenous analgesia

Expression of Tas1 Taste Receptors in Mammalian Spermatozoa: Functional Role of Tas1r1 in Regulating Basal Ca2+ and cAMP Concentrations in Spermatozoa

Background: During their transit through the female genital tract, sperm have to recognize and discriminate numerous chemical compounds. However, our current knowledge of the molecular identity of appropriate chemosensory receptor proteins in sperm is still rudimentary. Considering that members of the Tas1r family of taste receptors are able to discriminate between a broad diversity of hydrophilic chemosensory substances, the expression of taste receptors in mammalian spermatozoa was examined. Methodology/Principal Findings: The present manuscript documents that Tas1r1 and Tas1r3, which form the functional receptor for monosodium glutamate (umami) in taste buds on the tongue, are expressed in murine and human spermatozoa, where their localization is restricted to distinct segments of the flagellum and the acrosomal cap of the sperm head. Employing a Tas1r1-deficient mCherry reporter mouse strain, we found that Tas1r1 gene deletion resulted in spermatogenic abnormalities. In addition, a significant increase in spontaneous acrosomal reaction was observed in Tas1r1 null mutant sperm whereas acrosomal secretion triggered by isolated zona pellucida or the Ca2+ ionophore A23187 was not different from wild-type spermatozoa. Remarkably, cytosolic Ca2+ levels in freshly isolated Tas1r1-deficient sperm were significantly higher compared to wild-type cells. Moreover, a significantly higher basal cAMP concentration was detected in freshly isolated Tas1r1-deficient epididymal spermatozoa, whereas upon inhibition of phosphodiesterase or sperm capacitation, the amount of cAMP was not different between both genotypes. Conclusions/Significance: Since Ca2+ and cAMP control fundamental processes during the sequential process of fertilization, we propose that the identified taste receptors and coupled signaling cascades keep sperm in a chronically quiescent state until they arrive in the vicinity of the egg - either by constitutive receptor activity and/or by tonic receptor activation by gradients of diverse chemical compounds in different compartments of the female reproductive tract

Late Quaternary salinity variation in the Lake of Siebleben (Thuringia, Central Germany) - Methods of palaeoenvironmental analysis using Ostracoda and pollen

We test several methods of ostracod-based palaeoenvironmental reconstruction using indicator species approach, mutual ecological/climatic range methods, transfer functions, modern analogue technique and morphological variation within Cyprideis torosa in reconstructing the site evolution of a late Quaternary small lake basin in Thuringia, Central Germany. Sediment sections containing a diverse ostracod fauna were studied and compared with those from modern water bodies of Thuringia. Palynological investigations were executed to reconstruct the environmental conditions in the catchment area and for a obtaining a biostratigraphical framework. The brackish water ostracod Cyprideis torosa as well as the foraminifer Haplophragmoides indicate phases of saline groundwater influence, fed by salt bearing sediments of the Triassic basement. The accompanying freshwater ostracod fauna, however, reflects only low variations of salinity and temperature. Environmental changes in salinity, temperature and ecological stability indicated by microfossils and pollen are caused by an interplay of climatic shifts and the local geological and hydrological setting