57 research outputs found
Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga \u3ci\u3eNannochloropsis oceanica\u3c/i\u3e CCMP1779
Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular and genetic resources prerequisite for the rational engineering of marine algal feedstocks are lacking for most candidate species. Heterokonts of the genus Nannochloropsis naturally have high cellular oil content and are already in use for industrial production of high-value lipid products. First success in applying reverse genetics by targeted gene replacement makes Nannochloropsis oceanica an attractive model to investigate the cell and molecular biology and biochemistry of this fascinating organism group. Here we present the assembly of the 28.7 Mb genome of N. oceanica CCMP1779. RNA sequencing data from nitrogen-replete and nitrogendepleted growth conditions support a total of 11,973 genes, of which in addition to automatic annotation some were manually inspected to predict the biochemical repertoire for this organism. Among others, more than 100 genes putatively related to lipid metabolism, 114 predicted transcription factors, and 109 transcriptional regulators were annotated. Comparison of the N. oceanica CCMP1779 gene repertoire with the recently published N. gaditana genome identified 2,649 genes likely specific to N. oceanica CCMP1779. Many of these N. oceanica–specific genes have putative orthologs in other species or are supported by transcriptional evidence. However, because similarity-based annotations are limited, functions of most of these species-specific genes remain unknown. Aside from the genome sequence and its analysis, protocols for the transformation of N. oceanica CCMP1779 are provided. The availability of genomic and transcriptomic data for Nannochloropsis oceanica CCMP1779, along with efficient transformation protocols, provides a blueprint for future detailed gene functional analysis and genetic engineering of Nannochloropsis species by a growing academic community focused on this genus
Synthetic Strategies to Terpene Quinones/Hydroquinones
The cytotoxic and antiproliferative properties of many natural sesquiterpene-quinones and -hydroquinones from sponges offer promising opportunities for the development of new drugs. A review dealing with different strategies for obtaining bioactive terpenyl quinones/hydroquinones is presented. The different synthetic approches for the preparation of the most relevant quinones/hydroquinones are described
Antialiasing for Nonlinearities: Acoustic Modeling and Synthesis Applications
Nonlinear elements have manifold uses in acoustic modeling, audio synthesis and eects design. Of particular importance is their capacity to control oscillation dynamics in feedback models, and their ability to provide digital systems with a natural overdrive response. Unfortunately, nonlinearities are a major source of aliasing in a digital system. In this paper, alias suppression techniques are introduced which are particularly tailored to preserve response dynamics in acoustic models. To this end, a multirate framework for alias suppression is developed along with the concept of an aliasing signal-to-noise ratio (ASNR). Analysis of this framework proceeds as follows: rst, relations are established between ASNR vs. computational cost/delay given an estimate of the reconstructed output spectrum; second, techniques are given to estimate this spectrum in the worst case given only a few statistics of the input (amplitude, and bandwidth). These tools are used to show that hard circuit el..
An Investigation of Interrelations of Abilities in Guilford's Structure-of-Intellect
124 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1973.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD
A Flexible Analysis-Synthesis Method for Transients
We propose a new transient model which naturally extends the sinusoidal model to nonstationary phenomena. An integrated online-o#ine segmentation approach is developed, followed by an explicitly time-variant sinusoidal model. 1 Introduction The sinusoidal model is a convenient soundrepresentation in analysis-synthesis frameworks. The signal is modeled as a sum of sinusoids, with timevariant amplitudes and frequencies, plus a residual r(n), or unmodeled part: y(n) = 2p # k=1 A k,n cos( # m<n # k,m + # k,n ) + r n Such a parametric, time-frequency representation is ideal for analysis-synthesis goals: the parametric nature of the model allows meaningful specification of transformations on sound material, while the "timefrequency " nature facilitates intuition in developing musically viable transformations. Classical sinusoidal models; e.g. [MQ86] use STFT as front end. Implicit is the assumption of local stationarity. Estimation biases occur to the degree local stationarity i..
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