Acute Effects of Single Versus Combined Inhaled β2-Agonists Salbutamol and Formoterol on Time Trial Performance, Lung Function, Metabolic and Endocrine Variables

Background High prevalence rates of β2-agonist use among athletes in competitive sports makes it tempting to speculate that illegitimate use of β2-agonists boosts performance. However, data regarding the potential performance-enhancing effects of inhaled β2-agonists and its underlying molecular basis are scarce. Methods In total, 24 competitive endurance athletes (12f/12m) participated in a clinical double-blinded balanced four-way block cross-over trial to investigate single versus combined effects of β2-agonists salbutamol (SAL) and formoterol (FOR), to evaluate the potential performance enhancement of SAL (1200 µg, Cyclocaps, Pb Pharma GmbH), FOR (36 µg, Sandoz, HEXAL AG) and SAL + FOR (1200 µg + 36 µg) compared to placebo (PLA, Gelatine capsules containing lactose monohydrate, Pharmacy of the University Hospital Ulm). Measurements included skeletal muscle gene and protein expression, endocrine regulation, urinary/serum β2-agonist concentrations, cardiac markers, cardiopulmonary and lung function testing and the 10-min time trial (TT) performance on a bicycle ergometer as outcome variables. Blood and urine samples were collected pre-, post-, 3 h post- and 24 h post-TT. Results Mean power output during TT was not different between study arms. Treatment effects regarding lung function (p < 0.001), echocardiographic (left ventricular end-systolic volume p = 0.037; endocardial global longitudinal strain p < 0.001) and metabolic variables (e.g. NR4A2 and ATF3 pathway) were observed without any influence on performance. In female athletes, total serum β2-agonist concentrations for SAL and FOR were higher. Microarray muscle gene analysis showed a treatment effect for target genes in energy metabolism with strongest effect by SAL + FOR (NR4A2; p = 0.001). Of endocrine variables, follicle-stimulating hormone (3 h Post–Post-TT), luteinizing hormone (3 h Post–Pre-TT) and insulin (Post–Pre-TT) concentrations showed a treatment effect (all p < 0.05). Conclusions No endurance performance-enhancing effect for SAL, FOR or SAL + FOR within the permitted dosages compared to PLA was found despite an acute effect on lung and cardiac function as well as endocrine and metabolic variables in healthy participants. The impact of combined β2-agonists on performance and sex-specific thresholds on the molecular and cardiac level and their potential long-term performance enhancing or health effects have still to be determined. Trial registration: Registered at Eudra CT with the number: 2015-005598-19 (09.12.2015) and DRKS with number DRKS00010574 (16.11.2021, retrospectively registered)

Characterization of small planets with Kepler and HARPS-N

A. S. Bonomo, L. Malavolta, and X. Dumusque acknowledge fundings from the European Union Seventh Framework Programme (FP7/2007-2013) under agreement No. 313014 “Measuring ETAEARTH: characterization of terrestrial planetary systems with Kepler, HARPS-N, and Gaia” [PI: Dr. Alessandro Sozzetti].The high-accuracy and high-precision HARPS-N spectrograph has been installed at the italian Telescopio Nazionale Galileo in La Palma approximately two years and a half ago. Eighty nights per year of Guaranteed Time of Observation are mostly dedicated to the radial-velocity (RV) follow up of Kepler small-size planetary candidates to establish their nature and to determine accurately their masses. We report on recent results of this ongoing RV campaign, including the recent characterization of the planetary system Kepler-101.Publisher PD

High-precision photometry by telescope defocussing - VI. WASP-24, WASP-25 and WASP-26

The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013/) under grant agreement nos. 229517 and 268421. This publication was supported by grants NPRP 09-476-1-078 and NPRP X-019-1-006 from Qatar National Research Fund (a member of Qatar Foundation). TCH acknowledges financial support from the Korea Research Council for Fundamental Science and Technology (KRCF) through the Young Research Scientist Fellowship Programme and is supported by the KASI (Korea Astronomy and Space Science Institute) grant 2012-1-410-02/2013-9-400-00. SG, XW and XF acknowledge the support from NSFC under the grant no. 10873031. The research is supported by the ASTERISK project (ASTERoseismic Investigations with SONG and Kepler) funded by the European Research Council (grant agreement no. 267864). DR, YD, AE, FF (ARC), OW (FNRS research fellow) and J Surdej acknowledge support from the Communauté française de Belgique – Actions de recherche concertées – Académie Wallonie-Europe.We present time series photometric observations of 13 transits in the planetary systems WASP-24, WASP-25 and WASP-26. All three systems have orbital obliquity measurements, WASP-24 and WASP-26 have been observed with Spitzer, and WASP-25 was previously comparatively neglected. Our light curves were obtained using the telescope-defocussing method and have scatters of 0.5–1.2 mmag relative to their best-fitting geometric models. We use these data to measure the physical properties and orbital ephemerides of the systems to high precision, finding that our improved measurements are in good agreement with previous studies. High-resolution Lucky Imaging observations of all three targets show no evidence for faint stars close enough to contaminate our photometry. We confirm the eclipsing nature of the star closest to WASP-24 and present the detection of a detached eclipsing binary within 4.25 arcmin of WASP-26.Publisher PDFPeer reviewe

Transits and starspots in the WASP-6 planetary system

We present updates to PRISM, a photometric transit-starspot model, and GEMC, a hybrid optimization code combining MCMC and a genetic algorithm. We then present high-precision photometry of four transits in the WASP-6 planetary system, two of which contain a starspot anomaly. All four transits were modelled using PRISM and GEMC, and the physical properties of the system calculated. We find the mass and radius of the host star to be 0.836 ± 0.063 M⊙ and 0.864 ± 0.024 R⊙, respectively. For the planet, we find a mass of 0.485 ± 0.027 MJup, a radius of 1.230 ± 0.035 RJup and a density of 0.244 ± 0.014 ρJup. These values are consistent with those found in the literature. In the likely hypothesis that the two spot anomalies are caused by the same starspot or starspot complex, we measure the stars rotation period and velocity to be 23.80 ± 0.15 d and 1.78 ± 0.20 km s−1, respectively, at a colatitude of 75.8°. We find that the sky-projected angle between the stellar spin axis and the planetary orbital axis is λ = 7.2° ± 3.7°, indicating axial alignment. Our results are consistent with and more precise than published spectroscopic measurements of the Rossiter–McLaughlin effect. These results suggest that WASP-6 b formed at a much greater distance from its host star and suffered orbital decay through tidal interactions with the protoplanetary disc

The pro to toxin LYPD6B modulates heteromeric a3β4‐containing nicotinic acetylcholine receptors, but not α7 homomers

Prototoxins are a diverse family of membrane-tethered molecules expressed in the nervous system that modulate nicotinic cholinergic signaling, but their functions and specificity have yet to be completely explored. We tested the selectivity and efficacy of leukocyte antigen, PLAUR (plasminogen activator, urokinase receptor) domain-containing (LYPD)-6B on α3β4-, α3α5β4-, and α7-containing nicotinic acetylcholine receptors (nAChRs). To constrain stoichiometry, fusion proteins encoding concatemers of human α3, β4, and α5 (D and N variants) subunits were expressed in Xenopus laevis oocytes and tested with or without LYPD6B. We used the 2-electrode voltage-clamp method to quantify responses to acetylcholine (ACh): agonist sensitivity (EC(50)), maximal agonist-induced current (I(max)), and time constant (τ) of desensitization. For β4–α3–α3–β4–α3 and β4–α3–β4–α3–α3, LYPD6B decreased EC(50) from 631 to 79 μM, reduced I(max) by at least 59%, and decreased τ. For β4–α3–α5D–β4–α3 and β4–α3–β4–α–α5D, LYPD6B decreased I(max) by 63 and 32%, respectively. Thus, LYPD6B acted only on (α3)(3)(β4)(2) and (α3)(2)(α5D)(β4)(2) and did not affect the properties of (α3)(2)(β4)(3), α7, or (α3)(2)(α5N)(β4)(2) nAChRs. Therefore, LYPD6B acts as a mixed modulator that enhances the sensitivity of (α3)(3)(β4)(2) nAChRs to ACh while reducing ACh-induced whole-cell currents. LYPD6B also negatively modulates α3β4 nAChRs that include the α5D common human variant, but not the N variant associated with nicotine dependence.—Ochoa, V., George, A. A., Nishi, R., Whiteaker, P. The prototoxin LYPD6B modulates heteromeric α3β4-containing nicotinic acetylcholine receptors, but not α7 homomers

A detailed census of variable stars in the globular cluster NGC 6333 (M9) from CCD differential photometry

peer reviewedWe report CCD V and I time series photometry of the globular cluster NGC 6333 (M9). The technique of difference image analysis has been used, which enables photometric precision better than 0.05 mag for stars brighter than V ˜ 19.0 mag, even in the crowded central regions of the cluster. The high photometric precision has resulted in the discovery of two new RRc stars, three eclipsing binaries, seven long-term variables and one field RRab star behind the cluster. A detailed identification chart and equatorial coordinates are given for all the variable stars in the field of our images of the cluster. Our data together with the literature V-data obtained in 1994 and 1995 allowed us to refine considerably the periods for all RR Lyrae stars. The nature of the new variables is discussed. We argue that variable V12 is a cluster member and an Anomalous Cepheid. Secular period variations, double-mode pulsations and/or the Blazhko-like modulations in some RRc variables are addressed. Through the light-curve Fourier decomposition of 12 RR Lyrae stars we have calculated a mean metallicity of [Fe/H][SUB]ZW[/SUB] = -1.70 ± 0.01(statistical) ± 0.14(systematic) or [Fe/H]_{text{UVES}}=-1.67 ± 0.01(statistical) ± 0.19(systematic). Absolute magnitudes, radii and masses are also estimated for the RR Lyrae stars. A detailed search for SX Phe stars in the Blue Straggler region was conducted but none were discovered. If SX Phe exist in the cluster then their amplitudes must be smaller than the detection limit of our photometry. The colour-magnitude diagram has been corrected for heavy differential reddening using the detailed extinction map of the cluster of Alonso-García et al. This has allowed us to set the mean cluster distance from two independent estimates; from the RRab and RRc absolute magnitudes, we find 8.04 ± 0.19 and 7.88 ± 0.30 kpc, respectively

Petite édition de "L'Ecole des femmes" : modes, travaux féminins, variétés

27 juillet 18791879/07/27 (N2)-1879/07/27