Mapeando o desenvolvimento musical em alunos com as necessidades mais complexas: o projeto sounds of intent // Mapping musical Development in learners with the most complex needs: The Sounds of Intent Project

Este capítulo tem como foco o projeto Sounds of Intent, que visa mapear o desenvolvimento musical de jovens com as necessidades mais complexas. A partir de 2002, a equipe de pesquisa trabalhou com um grupo de profissionais que atuavam na área - musicoterapeutas, educadores musicais e outros profissionais - para desenvolver descrições precisas e interpretações compartilhadas das diferentes formas e níveis de engajamento musical que observaram entre crianças e jovens com necessidades educacionais especiais. O referencial teórico desenvolvido pela equipe é discutido nessa tradução. // This chapter focuses on the Sounds of Intent project, which aimed to map the musical development of young people with the most complex needs. Beginning in 2002, the research team collaborated with practitioners actively involved in the field—music therapists, music educators, and other professionals. Their objective was to develop accurate descriptions and shared interpretations of the different forms and levels of musical engagement observed among children and young people with complex needs. This translation discusses the theoretical framework developed by the team

Mapeando o desenvolvimento musical em alunos com as necessidades mais complexas: o projeto sounds of intent

This chapter focuses on the Sounds of Intent project, aimed mapping the musical development of young people with the most complex needs. From 2002, the research team worked with practitioners who were active in the field—music therapists, music educators, and other professionals—to develop accurate descriptions and shared interpretations of the different forms and levels of musical engagement they observed among children and young people with complex needs. The theoretical framework developed by the team is discussed in this translation.Este capítulo tem como foco o projeto Sounds of Intent, que visa mapear o desenvolvimento musical de jovens com as necessidades mais complexas. A partir de 2002, a equipe de pesquisa trabalhou com um grupo de profissionais que atuavam na área - musicoterapeutas, educadores musicais e outros profissionais - para desenvolver descrições precisas e interpretações compartilhadas das diferentes formas e níveis de engajamento musical que observaram entre crianças e jovens com necessidades educacionais especiais. O referencial teórico desenvolvido pela equipe é discutido nessa tradução

Detecção molecular de ovinos carreadores de Leptospira em ambiente tropical

The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.O objetivo do presente estudo foi analisar a aplicabilidade da PCR na detecção de ovinos carreadores de Leptospira em ambiente tropical. Brevemente, dois rebanhos ovinos, previamente reportados como sororeativo (A) e soronegativo (B) foram selecionados para este estudo. Da totalidade de animais de cada rebanho, amostras de urina e fluido vaginal (FV)/sêmen foram colhidas para cultura bacteriológica e PCR. Além disso, amostras de soro foram colhidas e utilizadas na sorologia (teste da soroaglutinação microscópica). Esta técnica confirmou o estado prévio dos dois rebanhos. Nenhuma amostra pura de leptospiras foi obtida no cultivo. Já na PCR, animais do Rebanho A apresentaram 26,7% (FV), 33,3% (sêmen) e 38,9% (urina) de amostras positivas. O Rebanho B apresentou 40,0% (FV), 33,3% (sêmen) e 5,6% (urina) de positividade pela PCR. Em conclusão, a PCR foi uma importante ferramenta na identificação de carreadores de leptospiras, incluindo animais do rebanho soronegativo, o que reforça as vantagens do uso desta técnica para a detecção de ovinos portadores como parte dos programas de controle da leptospirose em ambiente tropical.

Isolation and characterization of saprophytic and pathogenic strains of Leptospira from water sources in the Midwestern United States

The genus Leptospira is a diverse and unique group of bacteria comprising multiple saprophytic and pathogenic species, which survive and persist in suitable moist environments. Pathogenic species cause human and animal leptospirosis, a global and neglected zoonotic disease. Disease transmission occurs by exposure to contaminated water and moist soil environments or by contact with domestic animals and wildlife acting as reservoir hosts that shed Leptospira via urine. Here, we describe the unexpected diversity of saprophytic and pathogenic species of Leptospira isolated from water in the Midwestern United States. Samples were collected by volunteers in 11 counties in Iowa from water sources, including puddles, sewage, creeks, ponds, lakes, and rivers, during the summer of 2021. One hundred and five water samples were tested by culture for the presence of saprophytic and pathogenic species and by lipL32 qPCR specific for the detection of pathogens; 82 (78.1%) were culture positive and five (4.8%) were positive by lipL32 qPCR. Whole genome sequencing of isolates cultured from water samples identified 10 species of saprophytes, namely L. montravelensis, L. kemamanensis, L. bandrabouensis, L. bourretii, L. bouyouniensis, L. chreensis, L. ellinghausenii, L. terpstrae, L. yanagawae, and L. abararensis, as well as three novel saprophytic species. Whole genome sequencing also identified two novel pathogenic species. The remaining cultures comprised mixed populations of saprophytic species and six comprised a mixture of saprophytic and pathogenic species. One of these mixed cultures was enriched to select for a clonal isolate of pathogenic Leptospira, strain WS101.C1, which was classified as L. interrogans serogroup Djasiman serovar Djasiman. Cumulatively, 9.5% (10/105) of water samples were positive for pathogenic Leptospira. This study emphasizes the diversity of Leptospira present in water sources in the Midwestern United States and provides unique opportunities to explore the geographic diversity and evolution of this genus. The identification of known and novel pathogenic species circulating in local water sources highlights their potential usefulness as diagnostic antigens, as well as the role of water in the transmission of infection to human and animal populations. Integrating knowledge on human, animal, and environmental health is essential to control and predict risk for zoonoses

Mongooses (\u3ci\u3eUrva auropunctata\u3c/i\u3e) as reservoir hosts of leptospira species in the United States Virgin Islands, 2019–2020

During 2019–2020, the Virgin Islands Department of Health investigated potential animal reservoirs of Leptospira spp., the bacteria that cause leptospirosis. In this cross-sectional study, we investigated Leptospira spp. exposure and carriage in the small Indian mongoose (Urva auropunctata, syn: Herpestes auropunctatus), an invasive animal species. This study was conducted across the three main islands of the U.S. Virgin Islands (USVI), which are St. Croix, St. Thomas, and St. John. We used the microscopic agglutination test (MAT), fluorescent antibody test (FAT), real-time polymerase chain reaction (lipl32 rt-PCR), and bacterial culture to evaluate serum and kidney specimens and compared the sensitivity, specificity, positive predictive value, and negative predictive value of these laboratory meth-ods. Mongooses (n = 274) were live-trapped at 31 field sites in ten regions across USVI and humanely euthanized for Leptospira spp. testing. Bacterial isolates were sequenced and evaluated for species and phylogenetic analysis using the ppk gene. Anti-Leptospira spp. antibodies were detected in 34% (87/256) of mongooses. Reactions were observed with the following serogroups: Sejroe, Icterohaemorrhagiae, Pyrogenes, Mini, Cynopteri, Australis, Hebdomadis, Autumnalis, Mankarso, Pomona, and Ballum. Of the kidney specimens exam-ined, 5.8% (16/270) were FAT-positive, 10% (27/274) were culture-positive, and 12.4% (34/ 274) were positive by rt-PCR. Of the Leptospira spp. isolated from mongooses, 25 were L. borgpetersenii, one was L. interrogans, and one was L. kirschneri. Positive predictive values of FAT and rt-PCR testing for predicting successful isolation of Leptospira by culture were 88% and 65%, respectively. The isolation and identification of Leptospira spp. in mongooses highlights the potential role of mongooses as a wildlife reservoir of leptospirosis; mongooses could be a source of Leptospira spp. infections for other wildlife, domestic animals, and humans

Avaliação do impacto da leptospirose no desempenho atlético de equinos

Embora os sintomas mais freqüentemente descritos na leptospirose em equinos sejam febre, icterícia, nefrite e complicações oculares, pouco se estuda sobre a interferência desta infecção no desempenho atlético destes animais. O objetivo deste estudo foi avaliar a associação da sororeatividade para leptospirose com a ocorrência de hemorragia pulmonar induzida por exercício (HPIE) e com o desempenho atlético em equinos. Estudou-se 180 equinos adultos mantidos em treinamento no Jockey Clube Brasileiro do Rio de Janeiro para detecção de anticorpos anti-Leptospira pela técnica da soroaglutinação microscópica. Noventa (50,0%) animais se mostraram sororreativos com títulos = 200, com predomínio do sorotipo Copenhageni (87,8%). Os 79 animais sororreativos para Copenhageni foram avaliados por endoscopia das vias aéreas superiores 30 minutos após o exercício. Destes, 32 (40,5%) apresentavam histórico de dificuldade em ganhar peso, 24 (30,4%) inapetência e queda de desempenho atlético. Ao exame clínico, sete (7,8%) apresentaram icterícia, 18 (20,0%) lacrimejamento e hiperemia ótica, 24 (30,4%) encontravam-se apáticos, dois (2,5%) com mialgia, quatro (4,4%) com letargia e 33 (41,8%) com opacidade de pêlo. Na endoscopia das vias aéreas superiores 16 (20,2%) dos animais sororreativos apresentaram HPIE, assim como 10 (11,1%) dos equinos soronegativos para leptospirose. Dos 79 animais sororreativos para o serovar Copenhageni, 37 (41,1%) foram tratados com penicilina procainada associada à estreptomicina, e 15 dias após o tratamento verificou-se melhora significativa com relação à dificuldade em ganhar peso, inapetência, apatia e letargia. Já no que se refere à melhora do desempenho atlético e opacidade de pelo, as melhoras foram evidentes 45 dias após o tratamento. Sete destes animais apresentavam HPIE ao primeiro exame, e reverteram de grau 4 para 3 (dois animais) ou 2 (cinco animais). Verificou-se, portanto, associação entre sororreatividade para leptospirose com o desempenho atlético destes animais, principalmente no que diz respeito à severidade da HPIE, e que o tratamento específico foi capaz de reverter tal quadro

Infection by Leptospira spp. in cattle in a tropical region, Rio de Janeiro, Brazil

O artigo encontra-se em acesso aberto no site do Editor.Submitted by Repositório Arca ([email protected]) on 2019-03-07T16:36:36Z No. of bitstreams: 9 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) F173-1-s2.0-S0166093403002623-main.pdf: 134681 bytes, checksum: 820fec91603c61940cac3a994a78e78f (MD5) F175-1-s2.0-S0264410X03008740-main.pdf: 193775 bytes, checksum: c3c3fb64033df19bb8ef9fdfbb0cd6dc (MD5) F17-tropmed-92-210.pdf: 419874 bytes, checksum: 9cbdbdf865a4804a451c6b21dcccbec4 (MD5) F176-vol82(f2)_027-033.pdf: 263695 bytes, checksum: 21621d2776fd5183c22bac91b4a44408 (MD5) F171-1-s2.0-S0264410X07007943-main.pdf: 710083 bytes, checksum: 61528a759ab21910fb55a1362072e876 (MD5) F178-1-s2.0-S0304401708001507-main.pdf: 268550 bytes, checksum: d063f7f930864a167cd03335dbf16a74 (MD5) F172-zjv7493.pdf: 6383671 bytes, checksum: 7af31a63ac524501cf01323501531b4d (MD5) F174-1-s2.0-S0022175906000846-main.pdf: 310872 bytes, checksum: 7d4d0484c4f65b959ad7ce95f370e2ef (MD5)Approved for entry into archive by Priscila Nascimento ([email protected]) on 2019-07-22T17:21:42Z (GMT) No. of bitstreams: 9 F173-1-s2.0-S0166093403002623-main.pdf: 134681 bytes, checksum: 820fec91603c61940cac3a994a78e78f (MD5) F175-1-s2.0-S0264410X03008740-main.pdf: 193775 bytes, checksum: c3c3fb64033df19bb8ef9fdfbb0cd6dc (MD5) F17-tropmed-92-210.pdf: 419874 bytes, checksum: 9cbdbdf865a4804a451c6b21dcccbec4 (MD5) F176-vol82(f2)_027-033.pdf: 263695 bytes, checksum: 21621d2776fd5183c22bac91b4a44408 (MD5) F171-1-s2.0-S0264410X07007943-main.pdf: 710083 bytes, checksum: 61528a759ab21910fb55a1362072e876 (MD5) F178-1-s2.0-S0304401708001507-main.pdf: 268550 bytes, checksum: d063f7f930864a167cd03335dbf16a74 (MD5) F172-zjv7493.pdf: 6383671 bytes, checksum: 7af31a63ac524501cf01323501531b4d (MD5) F174-1-s2.0-S0022175906000846-main.pdf: 310872 bytes, checksum: 7d4d0484c4f65b959ad7ce95f370e2ef (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-07-22T17:21:42Z (GMT). No. of bitstreams: 9 F173-1-s2.0-S0166093403002623-main.pdf: 134681 bytes, checksum: 820fec91603c61940cac3a994a78e78f (MD5) F175-1-s2.0-S0264410X03008740-main.pdf: 193775 bytes, checksum: c3c3fb64033df19bb8ef9fdfbb0cd6dc (MD5) F17-tropmed-92-210.pdf: 419874 bytes, checksum: 9cbdbdf865a4804a451c6b21dcccbec4 (MD5) F176-vol82(f2)_027-033.pdf: 263695 bytes, checksum: 21621d2776fd5183c22bac91b4a44408 (MD5) F171-1-s2.0-S0264410X07007943-main.pdf: 710083 bytes, checksum: 61528a759ab21910fb55a1362072e876 (MD5) F178-1-s2.0-S0304401708001507-main.pdf: 268550 bytes, checksum: d063f7f930864a167cd03335dbf16a74 (MD5) F172-zjv7493.pdf: 6383671 bytes, checksum: 7af31a63ac524501cf01323501531b4d (MD5) F174-1-s2.0-S0022175906000846-main.pdf: 310872 bytes, checksum: 7d4d0484c4f65b959ad7ce95f370e2ef (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2015Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Veterinária. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Veterinária. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Veterinária. Niterói, RJ, Brasil.Universidade Federal da Bahia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil