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Laboratory evaluation of stable isotope labeling of Culicoides (Diptera: Ceratopogonidae) for adult dispersal studies.
BackgroundStable isotope labeling is a promising method for use in insect mark-capture and dispersal studies. Culicoides biting midges, which transmit several important animal pathogens, including bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), are small flies that develop in various semi-aquatic habitats. Previous Culicoides dispersal studies have suffered from the limitations of other labeling techniques, and an inability to definitively connect collected adult midges to specific immature development sites.ResultsAdult C. sonorensis were successfully labeled with 13C and 15N stable isotopes as larvae developing in a semi-aquatic mud substrate in the laboratory. High and low-dose isotope treatments for both elements significantly enriched midges above the background isotope levels of unenriched controls. Enrichment had no effect on C. sonorensis survival, though a slight (~â5 day) delay in emergence was observed, and there was no significant effect of pool size on 13C or 15N enrichment levels.ConclusionsStable isotope labeling is life-long, and does not interfere with natural insect behaviors. Stable isotope enrichment using 13C or 15N shows promise for Culicoides dispersal studies in the field. This method can be used to identify adult dispersal from larval source habitat where a midge developed. It may be possible to detect a single enriched midge in a pool of unenriched individuals, though further testing is needed to confirm the sensitivity of this method
Path Integral Monte Carlo Approach to the U(1) Lattice Gauge Theory in (2+1) Dimensions
Path Integral Monte Carlo simulations have been performed for U(1) lattice
gauge theory in (2+1) dimensions on anisotropic lattices. We extractthe static
quark potential, the string tension and the low-lying "glueball" spectrum.The
Euclidean string tension and mass gap decrease exponentially at weakcoupling in
excellent agreement with the predictions of Polyakov and G{\" o}pfert and Mack,
but their magnitudes are five times bigger than predicted. Extrapolations are
made to the extreme anisotropic or Hamiltonian limit, and comparisons are made
with previous estimates obtained in the Hamiltonian formulation.Comment: 12 pages, 16 figure
Effect of major school playground reconstruction on physical activity and sedentary behaviour: Camden active spaces.
BACKGROUND: The physical school environment is a promising setting to increase children's physical activity although robust evidence is sparse. We examined the effects of major playground reconstruction on physical activity and sedentary time in primary schools using a quasi-experimental design (comparison group pre-test/post-test design). METHODS: Five experimental and two control schools from deprived areas of inner city London were recruited at baseline. Main outcome was physical activity and sedentary time measured from objective monitoring (Actigraph accelerometer) at one year follow up. Pupils' impressions of the new playground were qualitatively assessed post construction. RESULTS: A total of 347 pupils (mean age = 8 years, 55% boys; 36% Caucasian) were recruited into the study at baseline; 303 provided valid baseline Actigraph data. Of those, 231 (76%) completed follow-up (n = 169 intervention; n = 62 control) and 77.4% of the sample recorded at least 4 days of Actigraph wear. In mixed models adjusted for age, sex, ethnicity, ratio activity or sedentary/wear time at baseline, wear time at follow up, and school, no differences were observed in total moderate - vigorous activity (B = -1.4, 95% CI, -7.1, 4.2 min/d), light activity (B = 4.1, 95% CI, -17.9, 26.1), or sedentary time (B = -3.8, 95% CI, -29.2, 21.6 min/d) between groups. There were significant age interactions for sedentary (p = 0.002) and light intensity physical activity (p = 0.008). We observed significant reductions in total sedentary (-28.0, 95% CI, -1.9, -54.1 min/d, p = 0.037) and increases in total light intensity activity (24.6, 95% CI, 0.3, 48.9 min/d, p = 0.047) for children aged under 9 yrs. old in the intervention. CONCLUSION: Major playground reconstruction had limited effects on physical activity, but reduced sedentary time was observed in younger children. Qualitative data suggested that the children enjoyed the new playgrounds and experienced a perceived positive change in well-being and social interactions
Phase diagram of the one-dimensional Holstein model of spinless fermions
The one-dimensional Holstein model of spinless fermions interacting with
dispersionless phonons is studied using a new variant of the density matrix
renormalisation group. By examining various low-energy excitations of finite
chains, the metal-insulator phase boundary is determined precisely and agrees
with the predictions of strong coupling theory in the anti-adiabatic regime and
is consistent with renormalisation group arguments in the adiabatic regime. The
Luttinger liquid parameters, determined by finite-size scaling, are consistent
with a Kosterlitz-Thouless transition.Comment: Minor changes. 4 pages, 4 figures. To appear in Physical Review
Letters 80 (1998) 560
Series Expansions for three-dimensional QED
Strong-coupling series expansions are calculated for the Hamiltonian version
of compact lattice electrodynamics in (2+1) dimensions, with 4-component
fermions. Series are calculated for the ground-state energy per site, the
chiral condensate, and the masses of `glueball' and positronium states.
Comparisons are made with results obtained by other techniques.Comment: 13 figure
Spectra of non-hermitian quantum spin chains describing boundary induced phase transitions
The spectrum of the non-hermitian asymmetric XXZ-chain with additional
non-diagonal boundary terms is studied. The lowest lying eigenvalues are
determined numerically. For the ferromagnetic and completely asymmetric chain
that corresponds to a reaction-diffusion model with input and outflow of
particles the smallest energy gap which corresponds directly to the inverse of
the temporal correlation length shows the same properties as the spatial
correlation length of the stationary state. For the antiferromagnetic chain
with both boundary terms, we find a conformal invariant spectrum where the
partition function corresponds to the one of a Coulomb gas with only magnetic
charges shifted by a purely imaginary and a lattice-length dependent constant.
Similar results are obtained by studying a toy model that can be diagonalized
analytically in terms of free fermions.Comment: LaTeX, 26 pages, 1 figure, uses ioplppt.st
Kosterlitz-Thouless transition in three-state mixed Potts ferro-antiferromagnets
We study three-state Potts spins on a square lattice, in which all bonds are
ferromagnetic along one of the lattice directions, and antiferromagnetic along
the other. Numerical transfer-matrix are used, on infinite strips of width
sites, . Based on the analysis of the ratio of scaled mass
gaps (inverse correlation lengths) and scaled domain-wall free energies, we
provide strong evidence that a critical (Kosterlitz-Thouless) phase is present,
whose upper limit is, in our best estimate, . From analysis
of the (extremely anisotropic) nature of excitations below , we argue that
the critical phase extends all the way down to T=0. While domain walls parallel
to the ferromagnetic direction are soft for the whole extent of the critical
phase, those along the antiferromagnetic direction seem to undergo a softening
transition at a finite temperature. Assuming a bulk correlation length varying,
for , as , , we attempt finite-size scaling plots of our finite-width
correlation lengths. Our best results are for . We propose a
scenario in which such inconsistency is attributed to the extreme narrowness of
the critical region.Comment: 11 pages, 6 .eps figures, LaTeX with IoP macros, to be published in J
Phys
COVID-19 knowledge and prevention behaviors in rural Zambia: a qualitative application of the information-motivation-behavioral skills model
In early 2020, the Zambian Ministry of Health instituted prevention guidelines to limit spread of COVID-19. We assessed community knowledge, motivations, behavioral skills, and perceived community adherence to prevention behaviors (i.e., hand hygiene, mask wearing, social distancing, and limiting gatherings). Within a cluster-randomized controlled trial in four rural districts, in November 2020 and May 2021, we conducted in-depth interviews with health center staff (N = 19) and community-based volunteers (N = 34) and focus group discussions with community members (N = 281). A content analysis was conducted in Nvivo v12. Data were interpreted using the Information-Motivation-Behavioral Skills Model. Generally, respondents showed good knowledge of COVID-19 symptoms, spread, and high-risk activities, with some gaps. Prevention behavior performance was driven by personal and social factors. Respondents described institutional settings (e.g., clinics and church) having higher levels of perceived adherence due to stronger enforcement measures and clear leadership. Conversely, informal community settings (e.g., weddings, funerals, football matches) lacked similar social and leadership expectations for adherence and had lower perceived levels of adherence. These settings often involved higher emotions (excitement or grief), and many involved alcohol use, resulting in community members "forgetting" guidelines. Doubt about disease existence or need for precautions persisted among some community members and drove non-adherence more generally. Although COVID-19 information successfully penetrated these very remote rural communities, more targeted messaging may address persistent COVID-19 doubt and misinformation. Engaging local leaders in religious, civic, and traditional leadership positions could improve community behaviors without adding additional monitoring duties on an already overburdened, resource-limited health system
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