Knowledge and Attitude of Dental Students and Staffs towards Basic life Support(BLS)

Introduction:The present study was aimed to assess the awareness, knowledge, and attitude towards basic life support (BLS) among the dental students and faculty of Al Azhar dental college. Materials and Methods:A descriptive study was conducted by using a Questionnaire comprising of 20 questions to collect the data pertaining to awareness and knowledge of BLS, attitude towards BLS among the 3rd, final year dental students and the faculty members. After excluding the incomplete response sheets which were none in the present study, the data from 212 members were subjected to the analysis. The Main outcome measure was the over all score in the BLS knowledge. Knowledge of BLS was assessed as per the data contained in the Basic life support manual from American Heart Association. Results: Out of 212 members 109 were 3rd year students and 85 were final year students and 18 faculty members. The overall knowledge score was 33.7%. Overall results were poor with less than minimum knowledge on the topic BLS. A score of less than 50% was evident in the study indicating a poor knowledge of BLS among both the students and the faculty.The range of correct answers were 3-11 among students and 4-6 among the staffs. A significant difference (

Mechanochemical syntheses, crystal structures, and photo-luminescent properties of a new hydrazone and its nickel and cadmium complexes

This work is funded by DST-SERB, India, Under EMEQ scheme (SB/EMEQ-164/2014).Peer reviewedPostprin

Exploiting the Metabolism of the Gut Microbiome as a Vehicle for Targeted Drug Delivery to the Colon

The prevalence of colon-associated diseases has increased significantly over the past several decades, as evidenced by accumulated literature on conditions such as Crohn’s disease, irritable bowel syndrome, colorectal cancer, and ulcerative colitis. Developing therapeutics for these diseases is challenging due to physiological barriers of the colon, systemic side effects, and the intestinal environment. Therefore, in a search for novel methods to overcome some of these problems, researchers discovered that microbial metabolism by gut microbiotia offers a potential method for targeted drug delivery This overview highlights several drug delivery systems used to modulate the microbiota and improve colon-targeted drug delivery. This technology will be important in developing a new generation of therapies which harness the metabolism of the human gut microflora

A solid-state infrared laser for two-step desorption–ionization processes in single-particle mass spectrometry

Recent advancements in single-particle mass spectrometry (SPMS) have enabled the detection of aromatic hydrocarbons at the individual particle level in conjunction with inorganic/refractory particle components. However, the laser desorption (LD) of organic material from particles prior to their ionization in a two-step process necessitates pulsed infrared lasers with adequate pulse energy that can be irregularly triggered on detected particles. Pulsed CO2 lasers with a 10.6 µm wavelength have been traditionally utilized, yet these lasers are bulky and costly and require regular maintenance, including gas exchange or a continuous laser gas supply. In this study, we present the application of a prototype solid-state laser based on an erbium-doped yttrium aluminum garnet (Er:YAG) crystal, emitting long pulses of 200 µs at 3 µm wavelength as a compact, cost-effective, and user-friendly alternative for LD. We directly compared the new laser with a commonly used CO2 laser and found similar performance in LD for both laboratory particles and ambient air experiments. With the exception of slightly increased fragmentation observed with the CO2 laser due to its beam profile, no qualitative differences were noted in the resulting mass spectra. Additionally, we compared a novel two-step ionization (LD-REMPI–LDI) with the conventional single-step LDI regarding the potential to detect polycyclic aromatic hydrocarbons (PAHs) and inorganics in laboratory and field experiments. The combined methods demonstrated superior performance in the detection of PAHs, for both the CO2 and the new Er:YAG laser. In addition to its higher sensitivity and lower fragmentation for PAHs when compared to single-step LDI, it is less dependent on the particle matrix, sharing the benefits of traditional two-step methods but extending its capability to combine PAH measurements with the LDI-based detection of inorganic particle compounds.</p

Neutrophil extracellular trap formation is independent of de novo gene expression

Neutrophils are essential innate immune cells whose responses are crucial in the clearance of invading pathogens. Neutrophils can respond to infection by releasing neutrophil extracellular traps (NETs). NETs are formed of chromatin and specific granular proteins and are released after execution of a poorly characterized cell death pathway. Here, we show that NET formation induced by PMA or Candida albicans is independent of RNA polymerase II and III-mediated transcription as well as of protein synthesis. Thus, neutrophils contain all the factors required for NET formation when they emerge from the bone marrow as differentiated cells

Therapeutic Potential of Cannabinoids on Tumor Microenvironment: A Molecular Switch in Neoplasia Transformation

The efficacy of chemotherapy depends on the tumor microenvironment. This microenvironment consists of a complex cellular network that can exert both stimulatory and inhibitory effects on tumor genesis. Given the increasing interest in the effectiveness of cannabis, cannabinoids have gained much attention as a potential chemotherapy drug. Cannabinoids are a group of marker compounds found in L., more commonly known as marijuana, a psychoactive drug used since ancient times for pain management. Although the anticancer potential of , has been recognized previously, increased attention was generated after discovering the endocannabinoid system and the successful production of cannabinoid receptors. In vitro and in vivo studies on various tumor models have shown therapeutic efficiency by modifying the tumor microenvironment. However, despite extensive attention regarding potential therapeutic implications of cannabinoids, considerable clinical and preclinical analysis is needed to adequately define the physiological, pharmacological, and medicinal aspects of this range of compounds in various disorders covered in this review. This review summarizes the key literature surrounding the role of cannabinoids in the tumor microenvironment and their future promise in cancer treatment

Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage

Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer

Biomass-burning sources control ambient particulate matter, but traffic and industrial sources control volatile organic compound (VOC) emissions and secondary-pollutant formation during extreme pollution events in Delhi

Volatile organic compounds (VOCs) and particulate matter (PM) are major constituents of smog. Delhi experiences severe smog during the post-monsoon season, but a quantitative understanding of VOCs and PM sources is still lacking. Here, we conduct a source apportionment study for VOCs and PM using a recent (2022), high-quality dataset of 111 VOCs, PM2.5, and PM10 in a positive matrix factorization (PMF) model. Contrasts between clean monsoon air and polluted post-monsoon air, VOC source fingerprints, and molecular tracers enabled us to differentiate paddy residue burning from other biomass-burning sources, which had previously been impossible. Burning of fresh paddy residue, as well as residential heating and waste burning, contributed the most to observed PM10 levels (25 % and 23 %, respectively) and PM2.5 levels (23 % and 24 %, respectively), followed by heavy-duty vehicles fuelled by compressed natural gas (CNG), with a PM10 contribution of 15 % and a PM2.5 contribution of 11 %. For ambient VOCs, ozone formation potential, and secondary-organic-aerosol (SOA) formation potential, the top sources were petrol four-wheelers (20 %, 25 %, and 30 %, respectively), petrol two-wheelers (14 %, 12 %, and 20 %, respectively), industrial emissions (12 %, 14 %, and 15 %, respectively), solid-fuel-based cooking (10 %, 10 %, and 8 %, respectively), and road construction (8 %, 6 %, and 9 %, respectively). Emission inventories tended to overestimate residential biofuel emissions at least by a factor of 2 relative to the PMF output. The major source of PM pollution was regional biomass burning, while traffic and industries governed VOC emissions and secondary-pollutant formation. Our novel source apportionment method even quantitatively resolved similar biomass and fossil fuel sources, offering insights into both VOC and PM sources affecting extreme pollution events. This approach represents a notable advancement compared to current source apportionment approaches, and it could be of great relevance for future studies in other polluted cities and regions of the world with complex source mixtures.</p

Neutrophil Extracellular Traps Directly Induce Epithelial and Endothelial Cell Death: A Predominant Role of Histones

Neutrophils play an important role in innate immunity by defending the host organism against invading microorganisms. Antimicrobial activity of neutrophils is mediated by release of antimicrobial peptides, phagocytosis as well as formation of neutrophil extracellular traps (NET). These structures are composed of DNA, histones and granular proteins such as neutrophil elastase and myeloperoxidase. This study focused on the influence of NET on the host cell functions, particularly on human alveolar epithelial cells as the major cells responsible for gas exchange in the lung. Upon direct interaction with epithelial and endothelial cells, NET induced cytotoxic effects in a dose-dependent manner, and digestion of DNA in NET did not change NET-mediated cytotoxicity. Pre-incubation of NET with antibodies against histones, with polysialic acid or with myeloperoxidase inhibitor but not with elastase inhibitor reduced NET-mediated cytotoxicity, suggesting that histones and myeloperoxidase are responsible for NET-mediated cytotoxicity. Although activated protein C (APC) did decrease the histone-induced cytotoxicity in a purified system, it did not change NET-induced cytotoxicity, indicating that histone-dependent cytotoxicity of NET is protected against APC degradation. Moreover, in LPS-induced acute lung injury mouse model, NET formation was documented in the lung tissue as well as in the bronchoalveolar lavage fluid. These data reveal the important role of protein components in NET, particularly histones, which may lead to host cell cytotoxicity and may be involved in lung tissue destruction