The Effects of Summer Vacation on Learning: A Look at Interrupted Instruction

The purpose of this study was to examine the effects of interrupted instruction, in the form of summer vacation, on reading achievement of high, middle, and low achieving students. The researcher determined the loss or gain by using Running Reading Records (RRR) from the ending of first grade moving to the beginning of second grade. A discussion of the relevance of year-round education (YRE) is included as well. This study revealed that 15 of the 62 students lost according to RRR levels while 47 of the 62 either maintained or gained levels. It was determined that interrupted instruction did not necessarily impede student performance in reading. Research on the topic of YRE shows very slight loss or gain in academic performance over time

Hsp90-binding immunophilins link p53 to dynein during p53 transport to the nucleus

The tumor suppressor protein p53 is known to be transported to the nucleus along microtubular tracks by cytoplasmic dynein. However, the connection between p53 and the dynein motor protein complex has not been established. Here, we show that hsp90.binding immunophilins link p53.hsp90 complexes to dynein and that prevention of that linkage in vivo inhibits the nuclear movement of p53. First, we show that p53.hsp90 heterocomplexes from DLD-1 human colon cancer cells contain an immunophilin (FKBP52, CyP-40, or PP5) as well as dynein. p53.hsp90.immunophilin.dynein complexes can be formed by incubating immunopurified p53 with rabbit reticulocyte lysate, and we show by peptide competition that the immunophilins link via their tetratricopeptide repeat domains to p53-bound hsp90 and by means of their PPIase domains to the dynein complex. The linkage of immunophilins to the dynein motor is indirect by means of the dynamitin component of the dynein-associated dynactin complex, and we show that purified FKBP52 binds directly by means of its PPIase domain to purified dynamitin. By using a temperature-sensitive mutant of p53 where cytoplasmic-nuclear movement occurs by shift to permissive temperature, we show that p53 movement is impeded when p53 binding to hsp90 is inhibited by the hsp90 inhibitor radicicol. Also, nuclear movement of p53 is inhibited when immunophilin binding to dynein is competed for by expression of a PPIase domain fragment in the same manner as when dynein linkage to cargo is dissociated by expression of dynamitin. This is the first demonstration of the linkage between an hsp90-chaperoned transcription factor and the system for its retrograde movement to the nucleus both in vitro and in vivo.Fil: Galigniana, Mario Daniel. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Harrell, Jennifer M.. University of Michigan; Estados UnidosFil: O´Hagen, Heather M.. University of Michigan; Estados UnidosFil: Ljungman, Mats. University of Michigan; Estados UnidosFil: Pratt, William B.. University of Michigan; Estados Unido

A rapid method for an offline glycerol determination during microbial fermentation

Background: The purpose of this work was to find a rapid method for glycerol detection during microbial fermentations. The method requirements were, first, to avoid sample pretreatment, and second, to measure glycerol precisely especially out of fermentation broth. Results: This was achieved by combining two reaction principles — the Malaprade reaction and the Hantzsch reaction. In the Malaprade reaction, glycerol is converted into formaldehyde. This forms a dye in the Hantzsch reaction after which adsorption is than detected. The subsequent assay was investigated with two different fermentation media, a chemically undefined and a chemically defined media, used for Pichia pastoris fermentation. In both media, as well as in real fermentation samples, glycerol content could be reproducibly detected with the method. Moreover, measurements were more precise than using a standard glycerol detection kit. Conclusions: With this rapid assay, glycerol could be detected easily in microbial fermentation broth. It is reliable over a wide concentration range including advantages such as an easy assay set-up, a short assay time and no sample pretreatment.Citation: Kuhn J, H Müller, D Salzig, P Czermak:A rapid method for an offline glycerol determination during microbial fermentation, Electronic Journal of Biotechnology 18 (2015) 3, 252-255 http://dx.doi.org/10.1016/j.ejbt.2015.01.00

Perspectives on Care Coordination for Youth with TBI: Moving Forward to Provide Better Care

BACKGROUND: Care coordination aligns services and optimizes outcomes for children with traumatic brain injury (TBI), yet numerous obstacles can impede effective care coordination following a TBI. OBJECTIVE: The goal of this work is to identify barriers and facilitators to care coordination from the perspective of individuals who care for young people impacted by TBI. METHODS: Twenty-one care providers participated in semi-structured interviews to gather their perspectives on systems of care coordination for youth with TBI and potential areas for improvement. Using reflexive thematic analysis, researchers identified key themes across interviews. RESULTS: Three themes were identified: 1) gaps in knowledge; 2) poor collaboration and communication between systems and care providers; and 3) inadequate legislative and policy frameworks that fund and support pediatric TBI. Across themes, participants shared their experiences and ideas to improve each of these areas. CONCLUSIONS: A structured, consistent, and coordinated system of care for pediatric TBI is critical to ensure optimal outcomes. Protocols that emphasize intentional and productive collaboration between healthcare settings and schools and education for all care providers are cornerstones in improving outcomes for children. Top-down action that develops policy and funding initiatives is needed to ensure equitable, consistent access to appropriate healthcare and educational supports

Working Through Hunter S. Thompson's Strange and Terrible Saga

The subjective and participatory method of New Journalism provides practitioner Hunter S. Thompson access to the kinds of creative, cultural   entrepreneurship seen in postwar American narratives. In his reported and written “work,” Thompson not only self-consciously performs class personas, but markets those identities as a model of enterprise through creative economy. Thompson’s critical perspective and status position approximates what sociologists call a “cultural omnivore,” someone who consumes all forms of culture, but who reproduces a position of privilege in doing so. In Hell’s Angels (1966), Thompson uses the privilege granted by omnivoracity to transform the limitations of the worker-writer into a commodifiable and safe identity that can access and reign over other social groups.  By prioritizing his status as “pro,” Thompson manipulates the symbolic and cultural capital of class identity, providing himself an opportunity to feature individual over collective politics. Yet to accomplish this, Thompson relies on representing—and exploiting—the working class. Thompson exemplifies the class performative aspect of a working persona that is able to attain cultural domination through the manipulation of working-class identities in literary markets

Using American Indian Legends to Teach Youths Financial Literacy: Innovative Approaches to Cultural Adaptation

In this article, we report on pilot implementation of a financial education program for American Indian (AI) youths. Our purpose is to share our experience engaging AI youths in a culturally relevant experience in which they learn financial education concepts. Specifically, we incorporated Ojibwe legends into lesson content to connect Ojibwe culture to the information being taught. We report a combination of quantitative survey data and qualitative observational notes that overall suggest evidence of success regarding effectively engaging AI youths in financial education. Our approach may be of particular interest to Extension educators working with youths from culturally underserved audiences

High-angle-of-attack pneumatic lag and upwash corrections for a hemispherical flow direction sensor

As part of the NASA F-14 high angle of attack flight test program, a nose mounted hemispherical flow direction sensor was calibrated against a fuselage mounted movable vane flow angle sensor. Significant discrepancies were found to exist in the angle of attack measurements. A two fold approach taken to resolve these discrepancies during subsonic flight is described. First, the sensing integrity of the isolated hemispherical sensor is established by wind tunnel data extending to an angle of attack of 60 deg. Second, two probable causes for the discrepancies, pneumatic lag and upwash, are examined. Methods of identifying and compensating for lag and upwash are presented. The wind tunnel data verify that the isolated hemispherical sensor is sufficiently accurate for static conditions with angles of attack up to 60 deg and angles of sideslip up to 30 deg. Analysis of flight data for two high angle of attack maneuvers establishes that pneumatic lag and upwash are highly correlated with the discrepancies between the hemispherical and vane type sensor measurements

Comparing stress, areas of stress and coping-strategies between distance-learning and on-campus students – A mixed-methods approach

In recent years, the increase in stress experienced by students, and the related health problems have become a key challenge for health psychologists. The aim of this cross-sectional survey study was to compare stress, areas of stress and coping-strategies of 246 distance-learning (81.7% female; 33.62 years, SD = 9.30) and 254 on-campus students (82.3% female; 24.23 years, SD = 3.99). One-way analyses of variance showed no significant differences in perceived stress and stress symptoms between the student groups. Stress-inducing areas were revealed by qualitative content analysis. Chi-square tests showed that on-campus students significantly more often reported study- and performance-related areas, whereas conflicts between work and private life were more present among distance-learning students. Results also indicated that on-campus students significantly more often cope with stress by means of social support. These findings may help tailoring stress-management interventions for different student groups

An integrated marine data collection for the German Bight – Part 1: Subaqueous geomorphology and surface sedimentology (1996–2016)

Datasets: Themengebiet - Geomorphologie - https://doi.org/10.48437/02.2020.K2.7000.0001Datasets: Themengebiet - Bathymetrie - https://doi.org/10.48437/02.2020.K2.7000.0002Datasets: Themengebiet - Sedimentologie - https://doi.org/10.48437/02.2020.K2.7000.000

Alternative Geometries for 3D Bioprinting of Calcium Phosphate Cement as Bone Substitute

In the literature, many studies have described the 3D printing of ceramic-based scaffolds (e.g., printing with calcium phosphate cement) in the form of linear structures with layer rotations of 90°, although no right angles can be found in the human body. Therefore, this work focuses on the adaptation of biological shapes, including a layer rotation of only 1°. Sample shapes were printed with calcium phosphate cement using a 3D Bioplotter from EnvisionTec. Both straight and wavy spokes were printed in a round structure with 12 layers. Depending on the strand diameter (200 and 250 µm needle inner diameter) and strand arrangement, maximum failure loads of 444.86 ± 169.39 N for samples without subsequent setting in PBS up to 1280.88 ± 538.66 N after setting in PBS could be achieved