Neurophysiological correlates of linearization in language production

<p>Abstract</p> <p>Background</p> <p>During speech production the planning of a description of several events requires, among other things, a verbal sequencing of these events. During this process, referred to as linearization during conceptualization, the speaker can choose between different types of temporal connectives such as 'Before' X did A, Y did B' or 'After' Y did B, X did A'. To capture the neural events of such linearization processes, event-related potentials (ERP) were measured in native speakers of German. Utterances were elicited by presenting a sequence of two pictures on a video screen. Each picture consists of an object that is associated with a particular action (e.g. book = reading). A coloured vocalization cue indicated to describe the sequence of two actions associated with the objects in chronological (e.g. red cue: 'After' I drove the car, I read a book) or reversed order (yellow cue).</p> <p>Results</p> <p>Brain potentials showed reliable differences between the two conditions from 180 ms after the onset of the vocalization prompt, with ERPs from the 'After' condition being more negative. This 'Before/After' difference showed a fronto-central distribution between 180 and 230 ms. From 300 ms onwards, a parietal distribution was observed. The latter effect is interpreted as an instance of the P300 response, which is known to be modulated by task difficulty.</p> <p>Conclusion</p> <p>ERPs preceding overt sentence production are sensitive to conceptual linearization. The observed early, more fronto-centrally distributed variation could be interpreted as involvement of working memory needed to order the events according to the instruction. The later parietal distributed variation relates to the complexity in linearization, with the non-chronological order being more demanding during the updating of the concepts in working memory.</p

Audiovisual Database with 360 Video and Higher-Order Ambisonics Audio for Perception, Cognition, Behavior, and QoE Evaluation Research

Research into multi-modal perception, human cognition, behavior, and attention can benefit from high-fidelity content that may recreate real-life-like scenes when rendered on head-mounted displays. Moreover, aspects of audiovisual perception, cognitive processes, and behavior may complement questionnaire-based Quality of Experience (QoE) evaluation of interactive virtual environments. Currently, there is a lack of high-quality open-source audiovisual databases that can be used to evaluate such aspects or systems capable of reproducing high-quality content. With this paper, we provide a publicly available audiovisual database consisting of twelve scenes capturing real-life nature and urban environments with a video resolution of 7680x3840 at 60 frames-per-second and with 4th-order Ambisonics audio. These 360 video sequences, with an average duration of 60 seconds, represent real-life settings for systematically evaluating various dimensions of uni-/multi-modal perception, cognition, behavior, and QoE. The paper provides details of the scene requirements, recording approach, and scene descriptions. The database provides high-quality reference material with a balanced focus on auditory and visual sensory information. The database will be continuously updated with additional scenes and further metadata such as human ratings and saliency information.Comment: 6 pages, 2 figures, accepted and presented at the 2022 14th International Conference on Quality of Multimedia Experience (QoMEX). Database is publicly accessible at https://qoevave.github.io/database

Towards the Perception of Sound Source Directivity Inside Six-Degrees-of-Freedom Virtual Reality

Sound source directivity is a measure of the distribution of sound, propagating from a source object. It is an essential component of how we perceive acoustic environments, interactions and events. For six-degrees-of-freedom (6-DoF) virtual reality (VR), the combination of binaural audio and complete freedom of movement introduces new influencing elements into how we perceive source directivity. This preliminary study aims to explore if factors attributed to 6- DoF VR have an impact on the way we perceive changes of simple sound source directivity. The study is divided into two parts. Part I comprises of a control experiment in a non-VR monaural listening environment. The task is to ascertain difference limen between reference and test signals using a method of adjustment test. Based on the findings in Part I, Part II implements maximum attenuation thresholds on the same sound source directivity patterns using the same stimuli in 6-DoF VR. Results indicate that for critical steady-state signals, factors introduced by 6-DoF VR potentially mask our ability to detect loudness differences. Further analysis of the behavioral data acquired during Part II provides more insight into how subjects assess sound source directivity in 6-DoF VR

Inference of Room Geometry From Acoustic Impulse Responses

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Listening Tests with Individual versus Generic Head-Related Transfer Functions in Six-Degrees-of-Freedom Virtual Reality

Individual head-related transfer functions (HRTFs) improve localization accuracy and externalization in binaural audio reproduction compared to generic HRTFs. Listening tests are often conducted using generic HRTFs due to the difficulty of obtaining individual HRTFs for all participants. This study explores the ramifications of the choice of HRTFs for critical listening in a six-degrees-of-freedom audio-visual virtual environment, when participants are presented with an overall audio quality evaluation task. The study consists of two sessions using either individual or generic HRTFs. A small effect between the sessions is observed in a condition where elevation cues are impaired. Other conditions are rated similarly between individual and generic HRTFs

An insight into common filtering in noisy simo blind system identification

ABSTRACT The effect of additive sensor noise on single-input-multiple-output (SIMO) blind system identification (BSI) algorithms based upon cross-relation (CR) error is investigated. Previous studies have shown that additive noise in the observed signal results in systems comprising the true estimated channels convolved with an erroneous &apos;common filter&apos;, and additionally that identification and removal of this filter significantly improves estimation error. However, the source of the common filter remained an open question. This paper explains the common filter through a first-order perturbation analysis of the CR matrix, showing that it be estimated from the perturbation and the eigenvectors of the noiseless CR matrix. The analysis given in this paper provides a new insight into the effect of noise on SIMO BSI algorithms and forms the first step towards an overall noise robust solution

Leukocyte Bim deficiency does not impact atherogenesis in ldlr -/- mice, despite a pronounced induction of autoimmune inflammation

Proapoptotic Bcl-2 family member Bim is particularly relevant for deletion of autoreactive and activated T and B cells, implicating Bim in autoimmunity. As atherosclerosis is a chronic inflammatory process with features of autoimmune disease, we investigated the impact of hematopoietic Bim deficiency on plaque formation and parameters of plaque stability. Bim−/− or wild type bone marrow transplanted ldlr−/− mice were fed a Western type diet (WTD) for 5 or 10 weeks, after which they were immunophenotyped and atherosclerotic lesions were analyzed. Bim−/− transplanted mice displayed splenomegaly and overt lymphocytosis. CD4+ and CD8+ T cells were more activated (increased CD69 and CD71 expression, increased interferon gamma production). B cells were elevated by 147%, with a shift towards the pro-atherogenic IgG-producing B2 cell phenotype, resulting in a doubling of anti-oxLDL IgG1 antibody titers in serum of bim−/− mice. Bim−/− mice displayed massive intraplaque accumulation of Ig complexes and of lesional T cells, although this did not translate in changes in plaque size or stability features (apoptotic cell and macrophage content). The surprising lack in plaque phenotype despite the profound pro-atherogenic immune effects may be attributable to the sharp reduction of serum cholesterol levels in WTD fed bim−/− mice

Piecewise-Linear Models of Genetic Regulatory Networks: Theory and Example

International audienceThe experimental study of genetic regulatory networks has made tremendous progress in recent years resulting in a huge amount of data on the molecular interactions in model organisms. It is therefore not possible anymore to intuitively understand how the genes and interactions together influence the behavior of the system. In order to answer such questions, a rigorous modeling and analysis approach is necessary. In this chapter, we present a family of such models and analysis methods enabling us to better understand the dynam-ics of genetic regulatory networks. We apply such methods to the network that underlies the nutritional stress response of the bacterium E. coli. The functioning and development of living organisms is controlled by large and complex networks of genes, proteins, small molecules, and their interactions, so-called genetic regulatory networks. The study of these networks has recently taken a qualitative leap through the use of modern genomic techniques that allow for the simultaneous measurement of the expression levels of all genes of an organism. This has resulted in an ever growing description of the interactions in the studied genetic regulatory networks. However, it is necessary to go beyond the simple description of the interactions in order to understand the behavior of these networks and their relation with the actual functioning of the organism. Since the networks under study are usually very large, an intuitive approach for their understanding is out of ques-tion. In order to support this work, mathematical and computer tools are necessary: the unambiguous description of the phenomena that mathematical models provide allows for a detailed analysis of the behaviors at play, though they might not exactly represent the exact behavior of the networks. In this chapter, we will be mostly interested in the modeling of the genetic reg-ulatory networks by means of differential equations. This classical approach allows precise numerical predictions of deterministic dynamic properties of genetic regu-latory networks to be made. However, for most networks of biological interest the application of differential equations is far from straightforward. First, the biochemi-cal reaction mechanisms underlying the interactions are usually not or incompletel

The prevalence of antibodies against the HLA-DRB3 protein in kidney transplantation and the correlation with HLA expression

Human leukocyte antigen (HLA)-DRB3 is a functional HLA class II gene, which has a limited allele diversity in the human population. Furthermore, the HLA-DRB3 gene is only present in a subset of individuals. Therefore, in organ transplantation, this HLA molecule is frequently mismatched between patient and graft donor and thus antibodies against this mismatched HLA molecule can develop. In this study, we aimed to evaluate the prevalence and reactivity of these antibodies and aimed to identify factors that underlie antibody formation against HLA-DRB3. We showed in our patient cohort that HLA-DRB3 antibodies are identified in about 7% of all patients that were screened with solid phase assays. In these assays, we observed multiple antibody reactivity patterns indicating that HLA-DRB3 harbours multiple epitopes. In those cases, where we succeeded at tracing back the induction of these antibodies to the molecular HLA typing of the immunogenic event, we noticed a different frequency of HLA-DRB1 allele groups in the donors as compared to a control group. To a certain extent this distribution (e.g. HLA-DRB1* 11 individuals) could be linked to an altered expression level. However, it also appears that different HLA-DRB3 alleles (e.g. HLA-DRB3* 01 group) vary in their immunogenicity without having an expression difference. In conclusion, our study provides information on the immunogenicity and reactivity patterns of antibodies against HLA-DRB3 in kidney transplantation, and it points towards the possibility of HLA expression as a factor underlying antibody formation

Msx1 and Msx2 are required for endothelial-mesenchymal transformation of the atrioventricular cushions and patterning of the atrioventricular myocardium

<p>Abstract</p> <p>Background</p> <p><it>Msx1 </it>and <it>Msx2</it>, which belong to the highly conserved <it>Nk </it>family of homeobox genes, display overlapping expression patterns and redundant functions in multiple tissues and organs during vertebrate development. <it>Msx1 </it>and <it>Msx2 </it>have well-documented roles in mediating epithelial-mesenchymal interactions during organogenesis. Given that both <it>Msx1 </it>and <it>Msx2 </it>are crucial downstream effectors of Bmp signaling, we investigated whether <it>Msx1 </it>and <it>Msx2 </it>are required for the Bmp-induced endothelial-mesenchymal transformation (EMT) during atrioventricular (AV) valve formation.</p> <p>Results</p> <p>While both <it>Msx1-/- </it>and <it>Msx2-/- </it>single homozygous mutant mice exhibited normal valve formation, we observed hypoplastic AV cushions and malformed AV valves in <it>Msx1-/-; Msx2-/- </it>mutants, indicating redundant functions of <it>Msx1 </it>and <it>Msx2 </it>during AV valve morphogenesis. In <it>Msx1/2 </it>null mutant AV cushions, we found decreased Bmp2/4 and <it>Notch1 </it>signaling as well as reduced expression of <it>Has2</it>, <it>NFATc1 </it>and <it>Notch1</it>, demonstrating impaired endocardial activation and EMT. Moreover, perturbed expression of chamber-specific genes <it>Anf</it>, <it>Tbx2</it>, <it>Hand1 </it>and <it>Hand2 </it>reveals mispatterning of the <it>Msx1/2 </it>double mutant myocardium and suggests functions of <it>Msx1 </it>and <it>Msx2 </it>in regulating myocardial signals required for remodelling AV valves and maintaining an undifferentiated state of the AV myocardium.</p> <p>Conclusion</p> <p>Our findings demonstrate redundant roles of <it>Msx1 </it>and <it>Msx2 </it>in regulating signals required for development of the AV myocardium and formation of the AV valves.</p