35 research outputs found
ADAMTS13 phenotype in plasma from normal individuals and patients with thrombotic thrombocytopenic purpura
The activity of ADAMTS13, the von Willebrand factor cleaving protease, is deficient in patients with thrombotic thrombocytopenic purpura (TTP). In the present study, the phenotype of ADAMTS13 in TTP and in normal plasma was demonstrated by immunoblotting. Normal plasma (nâ=â20) revealed a single band at 190Â kD under reducing conditions using a polyclonal antibody, and a single band at 150Â kD under non-reducing conditions using a monoclonal antibody. ADAMTS13 was not detected in the plasma from patients with congenital TTP (nâ=â5) by either antibody, whereas patients with acquired TTP (nâ=â2) presented the normal phenotype. Following immunoadsorption of immunoglobulins, the ADAMTS13 band was removed from the plasma of the patients with acquired TTP, but not from that of normal individuals. This indicates that ADAMTS13 is complexed with immunoglobulin in these patients. The lack of ADAMTS13 expression in the plasma from patients with hereditary TTP may indicate defective synthesis, impaired cellular secretion, or enhanced degradation in the circulation. This study differentiated between normal and TTP plasma, as well as between congenital and acquired TTP. This method may, therefore, be used as a complement in the diagnosis of TTP
Histomorphometric evaluation of strontium-containing nanostructured hydroxyapatite as bone substitute in sheep
Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats
Evolution of the mineral deposits from LĂ„ngban, Sweden, as recorded from strontium isotope data
Correlation between in vitro and in vivo assessments of biomaterials for bone-regeneration â a multi centre study
Increased conjugation frequencies in clinical Enterococcus faecium strains harbouring the enterococcal surface protein gene esp
Staphylococcus aureus bloodstream infections: Diverging trends of meticillin-resistant and meticillin-susceptible isolates, EU/EEA, 2005 to 2018
Background: Invasive infections caused by
Staphylococcus aureus have high clinical and epidemiological relevance. It is therefore important to monitor the S. aureus trends using suitable methods. Aim:
The study aimed to describe the trends of bloodstream infections (BSI) caused by meticillin-resistant
S. aureus (MRSA) and meticillin-susceptible S. aureus
(MSSA) in the European Union (EU) and the European
Economic Area (EEA). Methods: Annual data on
S. aureus BSI from 2005 to 2018 were obtained from
the European Antimicrobial Resistance Surveillance
Network (EARS-Net). Trends of BSI were assessed at
the EU/EEA level by adjusting for blood culture set
rate (number of blood culture sets per 1,000 days of
hospitalisation) and stratification by patient characteristics.Results: Considering a fixed cohort of laboratories consistently reporting data over the entire
study period, MRSA percentages among S. aureus
BSI decreased from 30.2% in 2005 to 16.3% in 2018.
Concurrently, the total number of BSI caused by
S. aureus increased by 57%, MSSA BSI increased by
84% and MRSA BSI decreased by 31%. All these trends
were statistically significant (p Conclusions:
The results indicate an increasing health burden of
MSSA BSI in the EU/EEA despite a significant decrease
in the MRSA percentage. These findings highlight the
importance of monitoring antimicrobial resistance
trends by assessing not only resistance percentages
but also the incidence of infections. Further research
is needed on the factors associated with the observed
trends and on their attributable risk
Revisiting mental hygiene: Josef Lundahlâs interpretation of modern psychiatry in Sweden at the beginning of the twentieth century
Multiple crustal sources for post-tectonic I-type granites in the Hercynian Iberian Belt
A post-tectonic plutonic array of felsic I-type
granites crops out in the western Hercynian Iberian Belt.
Isotope (Sr, N d, Pb) data favour the absence of an important
input of juvenile magmas in late- to post- tectonic
Hercynian felsic magmatism in western Iberia, but suggest
a reworking of different crustal protoliths, including
oceanic metabasic rocks accreted to mid-to-Iower crustal
levels during the early stages of the collision. I-type granites
were derived from different meta-igneous protoliths ranging
from metabasic to felsic compositions depending on their
geographical position from the external (e.g. Galicia-N
Portugal, GNP) to the innermost continental areas (Spanish
Central System and Los Pedroches Batholiths). The GNP 1-
type pIu tons related to eo-Hercynian accretional terranes
have lower initial 87Sr/86Sr ratios, lower negative ΔNd
values, and higher 206PbP04Pb ratios than other I-type granites of the Central Iberian zone. These more isotopically
primitive Hercynian I-type granites are important in
tracking pre-Hercynian accreted oceanic lithosphere
terranes