Multiscale characterisation of the mechanical properties of austenitic stainless steel joints

A multiscale investigation was pursued in order to obtain the strain distribution and evolution during tensile testing both at the macro- and micro-scale for a diffusion bonded 316L stainless steel. The samples were designed for the purpose to demonstrate that the bond line properties were equal or better than the parent material in a sample geometry that was extracted from a larger component. The macroscopic stress-strain curves were coupled to the strain distributions using a camera-based 2D – Digital Image Correlation system. Results showed significant amount of plastic deformation predominantly concentrated in shear bands which were extended over a large region, crossing through the joint area. Yet it was not possible to be certain whether the joint has shown significant plastic deformation. In order to obtain the joints’ mechanical response in more detail, in situ micromechanical testing was conducted in the SEM chamber that allowed areas of 1x1 mm2 and 50x50 mm2 to be investigated. The size of the welded region was rather small to be accurately captured from the camera based DIC system. Therefore a microscale investigation was pursued where the samples were tested within an SEM chamber. Low magnification SEM imaging was utilised in order to cover a viewing area of 1 mm×1 mm while high magnification SEM imaging was employed to provide evidence of the occurrence of plastic deformation within the joint, at an area of just 50 μm×50 μm. The strain evolution over the microstructural level, within the joint and at the base material was obtained. The local strains were highly non-homogeneous through the whole test. Final failure occurred approximately 0.2 mm away from the joint. Large local strains were measured within the joint region, while SEM imaging showed that plastic deformation occurs via the formation of strong slip bands, followed by the activation of additional slip systems upon further plastic deformation which end up in additional slip bands to form on the surface. Plastic deformation occurred by slip and twinning mechanisms. Upon necking, significant out of plane deformations and slip deformation mechanisms were observed which suggested that plastic deformation was also happening at the last stages of damage evolution for the specific alloy. This was also evident from the large difference between the 600 MPa UTS stress value and the low stress values before final failure (which in many cases was below 30 MPa)

Roughening improves hydrogen embrittlement resistance of Ti-6Al-4V

Polished surfaces of Ti-6Al-4V, the most commonly used titanium alloy, were observed to suffer from hydride growth and associated embrittlement during hydrogen charging, whereas rough surfaces suffered no such susceptibility. Direct microscopic analyses of recombined hydrogen bubbles and thermal desorption spectroscopy (TDS) revealed that the surface roughening promotes recombination of atomic hydrogen to molecular hydrogen, in turn, reducing the relative amount of atomic hydrogen uptake. Subsurface time-of-flight secondary-ion mass spectrometry (ToF-SIMS) further revealed that the high defect density underneath the roughened surface impedes hydrogen diffusion into the bulk. These combined effects mean that, unexpectedly, roughening significantly reduces hydrogen uptake into Ti-6Al-4V and enhances its resistance against hydrogen embrittlement – all resulting from a simple surface treatment

Deformation-induced microstructural banding in TRIP steels

Microstructure inhomogeneities can strongly influence the mechanical properties of advanced high-strength steels in a detrimental manner. This study of a transformation-induced plasticity (TRIP) steel investigates the effect of pre-existing contiguous grain boundary networks (CGBNs) of hard second-phases and shows how these develop into bands during tensile testing using in situ observations in conjunction with digital image correlation (DIC). The bands form by the lateral contraction of the soft ferrite matrix, which rotates and displaces the CGBNs of second-phases and the individual features within them to become aligned with the loading direction. The more extensive pre-existing CGBNs that were before the deformation already aligned with the loading direction are the most critical microstructural feature for damage initiation and propagation. They induce micro-void formation between the hard second-phases along them, which coalesce and develop into long macroscopic fissures. The hard phases, retained austenite and martensite, were not differentiated as it was found that the individual phases do not play a role in the formation of these bands. It is suggested that minimizing the presence of CGBNs of hard second-phases in the initial microstructure will increase the formability

Anxiolytic Effects of the MCH1R Antagonist TPI 1361-17

Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide that acts on the MCH1 receptor. MCH1R is expressed widely throughout the brain, particularly in regions thought to be involved in the regulation of stress and emotional response. The role of MCH in anxiety has been controversial, however. Central administration of MCH has been reported to promote or reduce anxiety-like behaviors. The anxiolytic activity of several MCH1R antagonists has also been debated. To address this issue, we have tested whether TPI 1361-17, a highly specific and high affinity MCH1R antagonist, exerts anxiolytic effects in two commonly used models of anxiety, the elevated plus maze and the light–dark transition test. We show that this MCH1R antagonist exerts potent anxiolytic effects in both assays. Our study therefore supports previous studies indicating that MCH1R antagonists may be useful in the treatment of anxiety

Genome Analysis Reveals Interplay between 5′UTR Introns and Nuclear mRNA Export for Secretory and Mitochondrial Genes

In higher eukaryotes, messenger RNAs (mRNAs) are exported from the nucleus to the cytoplasm via factors deposited near the 5′ end of the transcript during splicing. The signal sequence coding region (SSCR) can support an alternative mRNA export (ALREX) pathway that does not require splicing. However, most SSCR–containing genes also have introns, so the interplay between these export mechanisms remains unclear. Here we support a model in which the furthest upstream element in a given transcript, be it an intron or an ALREX–promoting SSCR, dictates the mRNA export pathway used. We also experimentally demonstrate that nuclear-encoded mitochondrial genes can use the ALREX pathway. Thus, ALREX can also be supported by nucleotide signals within mitochondrial-targeting sequence coding regions (MSCRs). Finally, we identified and experimentally verified novel motifs associated with the ALREX pathway that are shared by both SSCRs and MSCRs. Our results show strong correlation between 5′ untranslated region (5′UTR) intron presence/absence and sequence features at the beginning of the coding region. They also suggest that genes encoding secretory and mitochondrial proteins share a common regulatory mechanism at the level of mRNA export

Meta-Analysis of Genome-Wide Association Studies and Network Analysis-Based Integration with Gene Expression Data Identify New Suggestive Loci and Unravel a Wnt-Centric Network Associated with Dupuytren’s Disease

Dupuytren´s disease, a fibromatosis of the connective tissue in the palm, is a common complex disease with a strong genetic component. Up to date nine genetic loci have been found to be associated with the disease. Six of these loci contain genes that code for Wnt signalling proteins. In spite of this striking first insight into the genetic factors in Dupuytren´s disease, much of the inherited risk in Dupuytren´s disease still needs to be discovered. The already identified loci jointly explain ~1% of the heritability in this disease. To further elucidate the genetic basis of Dupuytren´s disease, we performed a genome-wide meta-analysis combining three genome-wide association study (GWAS) data sets, comprising 1,580 cases and 4,480 controls. We corroborated all nine previously identified loci, six of these with genome-wide significance (p-value < 5x10-8). In addition, we identified 14 new suggestive loci (p-value < 10−5). Intriguingly, several of these new loci contain genes associated with Wnt signalling and therefore represent excellent candidates for replication. Next, we compared whole-transcriptome data between patient- and control-derived tissue samples and found the Wnt/β-catenin pathway to be the top deregulated pathway in patient samples. We then conducted network and pathway analyses in order to identify protein networks that are enriched for genes highlighted in the GWAS meta-analysis and expression data sets. We found further evidence that the Wnt signalling pathways in conjunction with other pathways may play a critical role in Dupuytren´s disease