Effects of Temperature on the Toxicity to the Aquatic Biota of Waste Discharges - A Compilation of the Literature

An extensive compiliation and general evaluation of the literature is presented which describes the temperature interaction with toxicity. Recent literature is summarized and made accessible along with a few generalized relationships such that researchers may design studies in a manner that will increase the utility of their results. A detailed indexing system is employed which makes the information contained in the report accessible by author, toxicant, and text organism. Summary tables of the most pertinent literature are also presented for easy subject retrieval. It was concluded that very little uniformity in experimental design is found between experiments on temperature-toxicity relationships, and a generalized summary of the results presented in th eliterature is essentially impossible because of the inconsistencies in experimental designs. The utilization of standard bioassay procedures is highly recommened, and these procedures should be applied to experimental designs which allow the estimation of parameters related to the theoretical effects of temperature

Modelling pellet flow in single extrusion with DEM

Plasticating single-screw extrusion involves the continuous conversion of loose solid pellets into a pressurized homogeneous melt that is pumped through a shaping tool. Traditional analyses of the solids conveying stage assume the movement of an elastic solid plug at a fixed speed. However, not only the corresponding predictions fail considerably, but it is also well known that, at least in the initial screw turns, the flow of loose individual pellets takes place. This study follows previous efforts to predict the characteristics of such a flow using the discrete element method. The model considers the development of normal and tangential forces resulting from the inelastic collisions between the pellets and between them and the neighbouring metallic surfaces. The algorithm proposed here is shown to be capable of capturing detailed features of the granular flow. The predictions of velocities in the cross- and down-channel directions and of the coordination number are in good agreement with equivalent reported results. The effect of pellet size on the flow features is also discussed

Highly conductive p-type nanocrystalline silicon films deposited by RF-PECVD using silane and trimethylboron mixtures at high pressure

In this paper we present a study of boron-doped nc-Si:H films prepared by PECVD at high depositionpressure (>4 mbar), high plasma power and low substrate temperature (7 (U cm)1) andhigh optical band gap (>1.7 eV). Modeling of ellipsometry spectra reveals that the film growth mechanismshould proceed through a sub-surface layer mechanism that leads to silicon crystallization.The obtained films are very good candidates for application in amorphous and nanocrystalline siliconsolar cells as a p-type window layer.Peer reviewe

Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy

Deficiency of adenosine deaminase (ADA, EC3.5.4.4), a housekeeping enzyme intrinsic to the purine salvage pathway, leads to severe combined immunodeficiency (SCID) both in humans and mice. Lack of ADA results in the intracellular accumulation of toxic metabolites which have effects on T cell development and function. While untreated ADA-SCID is a fatal disorder, there are different therapeutic options available to restore ADA activity and reconstitute a functioning immune system, including enzyme replacement therapy (ERT). Administration of ERT in the form of pegylated bovine ADA (PEG-ADA) has proved a life-saving though non-curative treatment for ADA-SCID patients. However, in many patients treated with PEG-ADA, there is suboptimal immune recovery with low T and B cell numbers. Here, we show reduced thymus cellularity in ADA-SCID mice despite weekly PEG-ADA treatment. This was associated with lack of effective adenosine (Ado) detoxification in the thymus. We also show that thymocyte development in ADA-deficient thymi is arrested at the DN3-to-DN4 stage transition with thymocytes undergoing dATP-induced apoptosis rather than defective TCRβ rearrangement or β-selection. Our studies demonstrate at a detailed level that exogenous once-a-week enzyme replacement does not fully correct intra-thymic metabolic or immunological abnormalities associated with ADA deficiency

Peripheral biomarkers to diagnose obstructive sleep apnea in adults: A systematic review and meta-analysis

Background: Obstructive Sleep Apnea (OSA) has been recognized as a major health concern worldwide, given its increasing prevalence, difficulties in diagnosis and treatment, and impact on health, economy, and society. Clinical guidelines highlight the need of biomarkers to guide OSA clinical decision-making, but so far, without success. In this systematic review and meta-analysis, registered on the International Prospective Register of Systematic Reviews database (ID CRD42020132556), we proposed to gather and further explore candidates identified in the literature as potential OSA biomarkers. Methods: Search strategies for eight different databases (PubMed/Medline, Cochrane Library, Biblioteca Virtual da Saúde, Web of Science, EMBASE, World Intellectual Property Organization database, and bioRxiV and medRxiV Preprint Servers) were developed. We identified studies exploring potential biomarkers of OSA, in peripheral samples of adults, with and without OSA, with no comorbidities defined in study inclusion criteria, published after the last systematic review and meta-analysis conducted on OSA biomarkers, until May 31st, 2020. Risk of bias was assessed through the 14-item Quality Assessment Tool for Diagnostic Accuracy Studies. Demographic, clinical, and candidate biomarkers' data were collected and analyzed via random effects meta-analyses. Findings: Among the 1512 unique studies screened, 120 met the inclusion criteria and 16 studies with low risk of bias were selected for meta-analyses. The selected 16 studies enrolled a total of 2156 participants, from which 1369 were diagnosed with OSA and 787 were disease-free controls. The assessed variables showed high heterogeneity. From the 38 biomarker candidates evaluated, only two were evaluated in more than one study. Most studies pinpointed candidates with more potential for OSA prognosis. ADAM29, FLRT2 and SLC18A3 mRNA levels in PBMCs, Endocan and YKL-40 levels in serum, and IL-6 and Vimentin levels in plasma revealed the most promising candidates for OSA diagnosis. Interpretation: Although the current systematic review and meta-analysis allowed us to identify candidates to further explore as potential biomarkers in future studies, it is evident that OSA biomarkers research is still at an early stage. Most findings derive from small-size single-center study cohorts and single-candidate studies. We point several gaps in current OSA biomarker research that may guide into new directions and approaches towards the identification of OSA biomarkers.info:eu-repo/semantics/publishedVersio

Significant locus and metabolic genetic correlations revealed in genome-wide association study of anorexia nervosa

OBJECTIVE: The authors conducted a genome-wide association study of anorexia nervosa and calculated genetic correlations with a series of psychiatric, educational, and metabolic phenotypes. METHOD: Following uniform quality control and imputation procedures using the 1000 Genomes Project (phase 3) in 12 case-control cohorts comprising 3,495 anorexia nervosa cases and 10,982 controls, the authors performed standard association analysis followed by a meta-analysis across cohorts. Linkage disequilibrium score regression was used to calculate genome-wide common variant heritability (single-nucleotide polymorphism [SNP]-based heritability [h2SNP]), partitioned heritability, and genetic correlations (rg) between anorexia nervosa and 159 other phenotypes. RESULTS: Results were obtained for 10,641,224 SNPs and insertion-deletion variants with minor allele frequencies >1% and imputation quality scores >0.6. The h2SNP of anorexia nervosa was 0.20 (SE=0.02), suggesting that a substantial fraction of the twin-based heritability arises from common genetic variation. The authors identified one genome-wide significant locus on chromosome 12 (rs4622308) in a region harboring a previously reported type 1 diabetes and autoimmune disorder locus. Significant positive genetic correlations were observed between anorexia nervosa and schizophrenia, neuroticism, educational attainment, and high-density lipoprotein cholesterol, and significant negative genetic correlations were observed between anorexia nervosa and body mass index, insulin, glucose, and lipid phenotypes. CONCLUSIONS: Anorexia nervosa is a complex heritable phenotype for which this study has uncovered the first genome-wide significant locus. Anorexia nervosa also has large and significant genetic correlations with both psychiatric phenotypes and metabolic traits. The study results encourage a reconceptualization of this frequently lethal disorder as one with both psychiatric and metabolic etiology

The Tucana/Horologium, Columba, AB Doradus, and Argus Associations: New Members and Dusty Debris Disks

We propose 35 star systems within ~70 pc of Earth as newly identified members of nearby young stellar kinematic groups; these identifications include the first A- and late-B type members of the AB Doradus moving group and field Argus Association. All but one of the 35 systems contain a bright solar- or earlier-type star that should make an excellent target for the next generation of adaptive optics (AO) imaging systems on large telescopes. AO imaging has revealed four massive planets in orbit around the {\lambda} Boo star HR 8799. Initially the planets were of uncertain mass due in large part to the uncertain age of the star. We find that HR 8799 is a likely member of the ~30 Myr old Columba Association implying planet masses ~6 times that of Jupiter. We consider Spitzer Space Telescope MIPS photometry of stars in the ~30 Myr old Tucana/Horologium and Columba Associations, the ~40 Myr old field Argus Association, and the ~70 Myr old AB Doradus moving group. The percentage of stars in these young stellar groups that display excess emission above the stellar photosphere at 24 and 70 \mu m wavelengths - indicative of the presence of a dusty debris disk - is compared with corresponding percentages for members of 11 open clusters and stellar associations with ages between 8 and 750 Myr, thus elucidating the decay of debris disks with time.Comment: Accepted for publication in Ap

Whole Genome Analysis of Alentejano Pigs with Contrasting Meat Quality Phenotypes

The Alentejano is a Mediterranean pig breed, found in southern Portugal, reared under extensive conditions and finished on grass and acorns during the fall and winter months. From these animals a variety of dry-cured meat products of great economic importance are generated. A total of 541 pigs were studied during the 2017 slaughter campaign. Phenotypic records for carcass and meat quality were collected and subsequently analyzed to identify the groups of animals that displayed the most contrasting phenotypes. Two groups comprising 13 animals each were selected, based on pH, water loss, total lipids, total protein, total collagen and pigments content. All samples were re-sequenced to a 23x coverage. The reads were aligned to the pig genome and SNPs and structural variants identified between the two groups of animals. A total of 13,418,254 SNPs were identified, of which 6,851,475 and 6,566,779 were located in the genic and intergenic genomic regions, respectively. The number of SNPs for which at least 25 samples were present comprised 88.7%. The set of genic SNPs included 43,405 exonic non-synonymous SNPs and 60,750 exonic synonymous SNPs. The remaining SNPs were located in introns and ncRNA regions. Interestingly, SNPs with markedly different allele frequencies between the groups were also identified (a total of 230 SNPs with allele frequency differences between the groups of at least 30% or 70%). This study represents the first major characterization of Alentejano pigs at the genome level, and identified a significant number of SNPs potentially associated with meat quality

Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants

Mutations and inadequate methylation profiles of CITED2 are associated with human congenital heart disease (CHD). In mouse, Cited2 is necessary for embryogenesis, particularly for heart development, and its depletion in embryonic stem cells (ESC) impairs cardiac differentiation. We have now determined that Cited2 depletion in ESC affects the expression of transcription factors and cardiopoietic genes involved in early mesoderm and cardiac specification. Interestingly, the supplementation of the secretome prepared from ESC overexpressing CITED2, during the onset of differentiation, rescued the cardiogenic defects of Cited2-depleted ESC. In addition, we demonstrate that the proteins WNT5A and WNT11 held the potential for rescue. We also validated the zebrafish as a model to investigate cited2 function during development. Indeed, the microinjection of morpholinos targeting cited2 transcripts caused developmental defects recapitulating those of mice knockout models, including the increased propensity for cardiac defects and severe death rate. Importantly, the co-injection of anti-cited2 morpholinos with either CITED2 or WNT5A and WNT11 recombinant proteins corrected the developmental defects of Cited2-morphants. This study argues that defects caused by the dysfunction of Cited2 at early stages of development, including heart anomalies, may be remediable by supplementation of exogenous molecules, offering the opportunity to develop novel therapeutic strategies aiming to prevent CHD.Agência financiadora: Fundação para a Ciência e a Tecnologia (FCT) Comissão de Coordenação e Desenvolvimento Regional do Algarve (CCDR Algarve) ALG-01-0145-FEDER-28044; DFG 568/17-2 Algarve Biomedical Center (ABC) Municipio de Louléinfo:eu-repo/semantics/publishedVersio

Sex differences in intestinal carbohydrate metabolism promote food intake and sperm maturation

Physiology and metabolism are often sexually dimorphic, but the underlying mechanisms remain incompletely understood. Here, we use the intestine of Drosophila melanogaster to investigate how gut-derived signals contribute to sex differences in whole-body physiology. We find that carbohydrate handling is male-biased in a specific portion of the intestine. In contrast to known sexual dimorphisms in invertebrates, the sex differences in intestinal carbohydrate metabolism are extrinsically controlled by the adjacent male gonad, which activates JAK-STAT signalling in enterocytes within this intestinal portion. Sex reversal experiments establish roles for this malebiased intestinal metabolic state in controlling food intake and sperm production through gutderived citrate. Our work uncovers a male gonad-gut axis coupling diet and sperm production, and reveals that metabolic communication across organs is physiologically significant. The instructive role of citrate in inter-organ communication may be significant in more biological contexts than previously recognised