Use of pooled serum samples to assess herd disease status using commercially available ELISAs

Pooled samples are used in veterinary and human medicine as a cost-effective approach to monitor disease prevalence. Nonetheless, there is limited information on the effect of pooling on test performance, and research is required to determine the appropriate number of samples which can be pooled. Therefore, this study aimed to evaluate the use of pooled serum samples as a herd-level surveillance tool for infectious production-limiting diseases: bovine viral diarrhoea (BVD), infectious bovine rhinotracheitis (IBR), enzootic bovine leukosis (EBL) and Neospora caninum (NC), by investigating the maximum number of samples one can pool to identify one positive animal, using commercial antibody-detection ELISAs. Four positive field standards (PFS), one for each disease, were prepared by pooling highly positive herd-level samples diagnosed using commercially available ELISA tests. These PFS were used to simulate 18 pooled samples ranging from undiluted PFS to a dilution representing 1 positive in 1,000 animals using phosphate-buffered saline as diluent. A 1:10 dilution of the PFS resulted in positive results for IBR, BVD and EBL. Moreover, for IBR and BVD, results were still positive at 1:100 and 1:30 dilutions, respectively. However, for NC, a lower dilution (8:10) was required for a seropositive result. This study indicates that, at herd-level, the use of pooled serum is a useful strategy for monitoring infectious diseases (BVD, IBR and EBL) but not NC, using readily available diagnostic assays

The Geomechanics of CO2 Storage in Deep Sedimentary Formations

This paper provides a review of the geomechanics and modeling of geomechanics associated with geologic carbon storage (GCS), focusing on storage in deep sedimentary formations, in particular saline aquifers. The paper first introduces the concept of storage in deep sedimentary formations, the geomechanical processes and issues related with such an operation, and the relevant geomechanical modeling tools. This is followed by a more detailed review of geomechanical aspects, including reservoir stress-strain and microseismicity, well integrity, caprock sealing performance, and the potential for fault reactivation and notable (felt) seismic events. Geomechanical observations at current GCS field deployments, mainly at the In Salah CO2 storage project in Algeria, are also integrated into the review. The In Salah project, with its injection into a relatively thin, low-permeability sandstone is an excellent analogue to the saline aquifers that might be used for large scale GCS in parts of Northwest Europe, the U.S. Midwest, and China. Some of the lessons learned at In Salah related to geomechanics are discussed, including how monitoring of geomechanical responses is used for detecting subsurface geomechanical changes and tracking fluid movements, and how such monitoring and geomechanical analyses have led to preventative changes in the injection parameters. Recently, the importance of geomechanics has become more widely recognized among GCS stakeholders, especially with respect to the potential for triggering notable (felt) seismic events and how such events could impact the long-term integrity of a CO{sub 2} repository (as well as how it could impact the public perception of GCS). As described in the paper, to date, no notable seismic event has been reported from any of the current CO{sub 2} storage projects, although some unfelt microseismic activities have been detected by geophones. However, potential future commercial GCS operations from large power plants will require injection at a much larger scale. For such largescale injections, a staged, learn-as-you-go approach is recommended, involving a gradual increase of injection rates combined with continuous monitoring of geomechanical changes, as well as siting beneath a multiple layered overburden for multiple flow barrier protection, should an unexpected deep fault reactivation occur

A characterization of Claw-free CIS graphs and new results on the order of CIS graphs

A graph is CIS if every maximal clique interesects every maximal stable set. Currently, no good characterization or recognition algorithm for the CIS graphs is known. We characterize graphs in which every maximal matching saturates all vertices of degree at least two and use this result to give a structural, efficiently testable characterization of claw-free CIS graphs. We answer in the negative a question of Dobson, Hujdurovi\'c, Milani\v{c}, and Verret [Vertex-transitive CIS graphs, European J. Combin. 44 (2015) 87-98] asking whether the number of vertices of every CIS graph is bounded from above by the product of its clique and stability numbers. On the positive side, we show that the question of Dobson et al. has an affirmative answer in the case of claw-free graphs

Improved selection of the functional unit in environmental impact assessment of cement

The outcome of Life Cycle Assessment (LCA) studies is strongly influenced by the selection of the functional unit, which must be defined in such a way that, when products are compared, the amounts compared have identical utilities. LCA studies of cements mostly use mass based functional units (MFU). However, since different cements have a different composition and binding capacity, they result in different compressive strength and durability in their applications, so that MFUs are inadequate. Therefore, this study introduces as functional unit the Cement Functional Performance (CFP): mass of cement needed to obtain 1 MPa of compressive strength during one year of durability (kg/(MPa∙year. To evaluate the effect of the CFP, the environmental performance of three cements is compared. Results show that adding 5% of zeolite to cement results in a similar strength and 1.78 times higher durability of the produced concrete, which is explained by the reduction of chloride diffusion. Moreover, adding 19% of zeolite to cement increases its durability 2.75 times, but reduces compressive strength by 29%. As a result, cement with 5 and 19% of zeolite added has respectively 54 and 36% of the environmental impacts of Portland cement.status: publishe

Detección, diagnóstico y registro oportuno de enfermedad profesional en UNIMEC EPS. S.A.

Los empleadores deben implantar programas de salud ocupacional y sistemas de vigilancia epidemiológica para que al momento de sospechar la ocurrencia de una enfermedad profesional la reporten a la entidad promotora de salu