On-farm Diversity Assessment and Evaluation of Finger Millet Genotypes in the Mid Hills of Nepal

The objective of the study is to assess the phenotypic diversity among the finger millet genotypes and evaluate overall performance for the recommendation of genotypes to the farming community of mid hills of Nepal. The overall performance of 8 candidate genotypes selected  from diversity block of 46 varieties in 2015, were assessed in randomized complete block design (RCBD) with three replicates under on-farm conditions in Lamjung district during 2016. Highly significant (p?0.01) variability among the selected 8 genotypes for agro-morphological traits viz., plant height, number of fingers per head, grain yield, 1000 grain weight, straw yield, days to heading and maturity was revealed. Strong inter-correlation among grain yield, plant height, time to flowering and straw yield was detected. The traits viz., plant height, grain yield, straw yield, days to heading and days to maturity were the most important traits contributing to the overall variability and thus, provide options for selection

Germplasm rescue and repatriation

Due to many factors such as increase access and availability of modern varieties, change in market preferences, low productivity of native landraces and climate change, crop landraces are threatened and are at the risk of losing from the fields. Still there are many rare and unique landraces conserved by farmers in different parts of the country. Native agricultural genetic resources that are being grown in red zone areas are all endangered. Unique and rare landraces as well as landraces grown in small areas by few farmers are also endangered. Different natural calamities also make native landraces endangered. Such landraces are lost if further conservation action did not take place. Exploration and collection of such endangered landraces is called germplasm rescue. National gene bank started rescue since 2014 for buckwheat diversity in Dolpa district

Participatory seed exchange (PSE): A community based mechanism for promoting access to seeds

Participatory Seed Exchange (PSE) is a low cost, simple and effective community-based mechanism for improving farmers' access to locally adapted seeds and planting materials which promotes farmer led on-farm conservation and utilization of the agrobiodiversity by exchanging available Agricultural Plant Genetic Resource (APGR) within the community (Shrestha et al 2013, Gautam et al 2017, Sthapit et al 2019). In Nepal, PSE was first piloted by the Western Terai Landscape Complex Project (WTLCP) in 2008 (Shrestha et al 2013). PSE is being utilized as a multi-propose tool to identify, exchange and document available APGR along with associated traditional knowledge by mobilizing local community and their networks. Though PSE is a one-day event, it takes an approximately a month-long time period to plan, prepare and practice

On-farm agrobiodiversity measurement and conservation

Total agrobiodiversity of any area is necessary to plan the implementation of agricultural and environmental projects and activities. Diversity is most for advancing agriculture development, however, modern agriculture has accelerated the replacement of old age crop diversity. Agrobiodiversity index and measures are commonly used and estimated for crop and animal species, landraces and sites. These are useful for locating sites, crops and custodians of agrobiodiversity. Agrobiodiversity includes crop and plant; livestock and fish, insect and microbial genetic resources that are cultivated, semi domesticated or wild. Diversity are ated properly that leads to choose the conservation approaches effectively

Diversifying the sourcing and deploying methods to enhance the crop diversity

A Nepalese farmers of mountain and hill agro-ecosystem mostly grow landraces or traditional varieties of most of the crops. In some major crops like rice, wheat and maize, very old varieties are in cultivation. Insufficient research on minor crops and poor extension networks to disseminate new varieties of major crops are the major reasons to narrow down the varietal options available to the farmers. Crop genetic diversity can make farming systems more resilient, but a major constraint is that farmers lack access to crop genetic resources (Tripp 1997). Farmers have fewer options available to choose, especially at a time when more new diversity is needed to cope with climate and market change (Atlin et al 2017). A portfolio of varieties exists in National Agricultural Genetic Resources Centre (Genebank) and many research stations that includes different varieties which are better than those currently grown by small farmers in remote hills and mountains, who have had limited opportunity to test these different options. In this context, potentials landraces sourced and collected in national Genebank from different environments can be deployed to the farmers of similar production environments

Hydrogeochemistry of Two Major Mid-hill Lentic Water Bodies for Irrigation of the Central Himalaya, Nepal

The concentration and composition of different salts in natural water bodies determine the water quality for various purposes. This study assesses the water quality of two mid-mountain lentic water bodies, Lake Phewa and Kulekhani Reservoir. For this purpose, selected physico-chemical parameters along with major ions such as HCO3-, SO42-, PO43-, NO3-, Cl-, Ca2+, Mg2+, Na+, K+, and NH4+ were analyzed. Major ions were analyzed using ion chromatography, anions by DX-600 and cations by Dionex ISC-2500 ion chromatographs. The sources of major ions were determined by using the Gibbs diagram, Piper plot, and Scatter plots. Dissolved oxygen, ammonia and phosphate showed seasonal variations in both lakes. The concentrations of cations are in the order of Ca2+ > Na+ > Mg2+ > K+ in both water bodies. However the trend of anions had small variations for Cl- and SO42- in Lake Phewa (HCO3- > Cl- > SO42- > NO3-) and Kulekhani Reservoir (HCO3- > SO42- > Cl- > NO3-). The Piper plot and equiline plots indicated that the water chemistry is dominantly controlled by the dissolution of carbonate minerals and to a limited extent by weathering of silicate minerals. This is further supported by the Gibbs plot showing bedrock geology as the main source of major ions. The overall study indicates that the hydrogeochemistry of these water bodies is controlled by local geology and is suitable for irrigation purposes

Community mobilisation and health management committee strengthening to increase birth attendance by trained health workers in rural Makwanpur, Nepal: study protocol for a cluster randomised controlled trial

Background: Birth attendance by trained health workers is low in rural Nepal. Local participation in improving health services and increased interaction between health systems and communities may stimulate demand for health services. Significant increases in birth attendance by trained health workers may be affected through community mobilisation by local women's groups and health management committee strengthening. We will test the effect of community mobilisation through women's groups, and health management committee strengthening, on institutional deliveries and home deliveries attended by trained health workers in Makwanpur District. Design: Cluster randomised controlled trial involving 43 village development committee clusters. 21 clusters will receive the intervention and 22 clusters will serve as control areas. In intervention areas, Female Community Health Volunteers are supported in convening monthly women's groups. The groups work through an action research cycle in which they consider barriers to institutional delivery, plan and implement strategies to address these barriers with their communities, and evaluate their progress. Health management committees participate in three-day workshops that use appreciative inquiry methods to explore and plan ways to improve maternal and newborn health services. Follow-up meetings are conducted every three months to review progress. Primary outcomes are institutional deliveries and home deliveries conducted by trained health workers. Secondary outcome measures include uptake of antenatal and postnatal care, neonatal mortality and stillbirth rates, and maternal morbidity

Genetic mechanisms of critical illness in COVID-19.

Host-mediated lung inflammation is present1, and drives mortality2, in the critical illness caused by coronavirus disease 2019 (COVID-19). Host genetic variants associated with critical illness may identify mechanistic targets for therapeutic development3. Here we report the results of the GenOMICC (Genetics Of Mortality In Critical Care) genome-wide association study in 2,244 critically ill patients with COVID-19 from 208 UK intensive care units. We have identified and replicated the following new genome-wide significant associations: on chromosome 12q24.13 (rs10735079, P = 1.65 × 10-8) in a gene cluster that encodes antiviral restriction enzyme activators (OAS1, OAS2 and OAS3); on chromosome 19p13.2 (rs74956615, P = 2.3 × 10-8) near the gene that encodes tyrosine kinase 2 (TYK2); on chromosome 19p13.3 (rs2109069, P = 3.98 ×  10-12) within the gene that encodes dipeptidyl peptidase 9 (DPP9); and on chromosome 21q22.1 (rs2236757, P = 4.99 × 10-8) in the interferon receptor gene IFNAR2. We identified potential targets for repurposing of licensed medications: using Mendelian randomization, we found evidence that low expression of IFNAR2, or high expression of TYK2, are associated with life-threatening disease; and transcriptome-wide association in lung tissue revealed that high expression of the monocyte-macrophage chemotactic receptor CCR2 is associated with severe COVID-19. Our results identify robust genetic signals relating to key host antiviral defence mechanisms and mediators of inflammatory organ damage in COVID-19. Both mechanisms may be amenable to targeted treatment with existing drugs. However, large-scale randomized clinical trials will be essential before any change to clinical practice