Microstructural and strength study of MIG welded joints of AW7020 aluminium alloy, as a function of joint geometry

[EN] Medium strength AW7XXX aluminium alloys are widely used as welded structures and in transportation. The applications of these alloys are limited by the behaviour of the welded joints. There is no agreement on the joint geometry that must be used on 5 mm welds. The microhardness evolution is one of the most important strength indicators. For this reason, the aim of this work is to study the influence of welded joint geometry on microhardness profile and on the microstructure of a MIG welded AW7020 aluminium alloy, using AW5256 filler.Bloem, C.; Salvador Moya, MD.; Amigó, V.; Vicente-Escuder, Á. (2000). Microstructural and strength study of MIG welded joints of AW7020 aluminium alloy, as a function of joint geometry. Welding International. 14(12):970-974. doi:10.1080/09507110009549300S970974141

Standardized endpoint definitions for transcatheter aortic valve implantation clinical trials: a consensus report from the Valve Academic Research Consortium†

To propose standardized consensus definitions for important clinical endpoints in transcatheter aortic valve implantation (TAVI), investigations in an effort to improve the quality of clinical research and to enable meaningful comparisons between clinical trials. To make these consensus definitions accessible to all stakeholders in TAVI clinical research through a peer reviewed publication, on behalf of the public health

Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores

Acknowledgements: S.E.S. acknowledges funding from Oxford Nanopore Technologies, Engineering and Physical Sciences Research Council (EPSRC) and Cambridge Trust. N.E.W. acknowledges funding from Oxford Nanopore Technologies, the Canada UK Foundation and the University of Cambridge Office of Postdoctoral Affairs. S.Y. acknowledges funding from the EPSRC (EP/S022953/1), and A.D. acknowledges funding from the EPSRC (EP/L015889/1). U.F.K. and K.C. acknowledge funding through the European Research Council (ERC-2019-POC PoreDetect 899538). We thank Z. Xuan and N. Ermann for assisting in the development of data analysis tools and C. Platnich for the helpful reading of the manuscript and useful suggestions.Funder: EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: ‘Ideas ’ Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013)); doi: https://doi.org/10.13039/100011199; Grant(s): ERC-2019-POC PoreDetect 899538, ERC-2019-POC PoreDetect 899538Funder: Oxford Nanopore Technologies (Oxford Nanopore); doi: https://doi.org/10.13039/100010890Funder: Cambridge Commonwealth, European and International Trust (Cambridge Commonwealth, European & International Trust); doi: https://doi.org/10.13039/501100003343Sequence-specific interactions between nucleic acids and proteins are fundamental to many critical biological processes. Despite the ubiquitous nature of protein-DNA binding, versatile methods to probe the specificity of these events remain elusive. In particular, single-molecule methods that enable the quantification of these processes are essential towards understanding and manipulating protein binding. To this end, we report a system which leverages solid state nanopores with diameters of ~10 nm to identify binding events between DNA and CRISPR associated (Cas) probes – specifically catalytically inactive or dead Cas9 (dCas9), which binds to DNA but does not cleave it. The rational design of DNA nanostructures allows for the incorporation of user-defined binding sequences, enabling a systematic study of how mismatch position and identity impacts the binding efficiency. These experiments reveal the relationship between sequence and binding at the single nucleotide level, exemplifying the utility of both nanopore measurements and DNA nanotechnology towards the next generation of biosensing assays.S.E.S. acknowledges funding from Oxford Nanopore Technologies, Engineering and Physical Sciences Research Council (EPSRC) and Cambridge Trust. N.E.W. acknowledges funding from Oxford Nanopore Technologies, the Canada UK Foundation, and the University of Cambridge Office of Postdoctoral Affairs. S.Y. acknowledges funding from the EPSRC grant EP/S022953/1 and A.D. acknowledges funding from the EPSRC grant EP/L015889/1. U.F.K and K.C. acknowledge funding through a ERC-2019-POC PoreDetect 899538