Membrane processing of grape must for control of the alcohol content in fermented beverages

The great demand of beverages, both alcohol-free and with low alcohol content, is a great challenge for the production of beverages with controlled alcohol content through the use of sustainable enological practices. The present work addresses this challenge with the processing of grape must by reverse osmosis (RO) for must reconstitution with different sugar contents prior to the alcoholic fermentation. The original must came from grapes grown in Quinta do Quinto, in Santarém, collected after destemming and mechanic crushing, and preserved in a refrigerated chamber at -1.6 °C until processing by RO. The RO processing was carried out in Escola Superior Agrária de Santarém, with a pilot plant equipped with RO spiral wound modules, M38RO, from Alfa Laval, Denmark. The total membrane permeation area is 15 m2. The work pressure was 55 bar. The original must had 23.7 oBrix, a density of 1108 g.L-1, 15.2% (v/v) of probable alcohol, and a conductivity of 2.01 mS.cm-1. The must reconstitution was carried out, by mixing the concentrated grape must with the vegetal water produced by RO (permeate) to obtain beverages with a nominal alcohol content of 5%, 7%, 10% and 13% (v/v). The fermentation average temperature was between 18.2 and 19.7 °C, and the final density rounded about 993 g.cm-3. The beverages were analysed by different parameters, including total polyphenols, total anthocyanins, colour intensity and hue, the coordinates CIELab, alcohol content, total acidity, volatile acidity, pH, free SO2 and total SO2. The attributes of the beverage, corresponding to the visual appearance, aroma and taste senses, as well as the overall judgment were evaluated by the tasters. The proposed method can produce beverages with controlled low alcohol content. The decrease of the alcohol content led to lower content of polyphenols compounds which influenced the sensory evaluation.info:eu-repo/semantics/publishedVersio

Diretrizes curriculares nacionais para a educação ambiental : considerações sobre a sua implementação no Brasil

O trabalho tem por objetivo investigar ideias de professores do ensino fundamental e médio sobre legislação, inserção no currículo e formação continuada em Educação Ambiental (EA), temas das Diretrizes Curriculares Nacionais para a EA. Esse documento, de junho de 2012, visa orientar a implantação do que é determinado pela Constituição Federal Brasileira e pela lei que dispõe sobre EA. Os resultados que emergiram das respostas a um questionário, foram utilizados para tecer considerações sobre a sua implementação no Brasil. Os professores parecem caminhar alheios à discussão das Diretrizes. Dada a precariedade do trabalho docente, corre-se o risco dessas serem "cumpridas", devido à pressão dos órgãos oficiais, com a inserção de conhecimentos pontuais, mensuráveis pelos atuais sistemas nacionais de avaliação, num trabalho alienado por parte dos professores

The effects of grazing management in montado fragmentation and heterogeneity

The Portuguese silvo-pastoral system montado is broadly classified as a High Nature Value (HNV) system since it corresponds to farmland hosting high biodiversity levels, and such biodiversity depends on specific land use practices. However, in recent decades a decline both in the totalmontado area and in the tree cover density within the montado has been observed, driven mainly by management changes. This decline may result in biodiversity loss. Grazing is a central aspect determining the long-term sustainability of the montado system and it has implications also on themontado structural diversity, particularly on connectivity and heterogeneity, which is crucial for the maintenance of montado HNV. The aim of this paper is to demonstrate how variations in montadostructural diversity are correlated with grazing management and its implications on the value of the system for conservation. The empirical data derives from a case study composed of 41 montadofarms in two municipalities of the Alentejo region. Data on grazing management, biophysical and spatial factors were collected and several metrics were calculated to assess montado fragmentation and heterogeneity. A multivariate analysis was performed using generalized additive models. Results show that different grazing patterns, depending on stocking density and grazing animal type, are correlated with variations in montado fragmentation and heterogeneity. Particularly, cattle’s grazing is shown to have adverse effects on the montado fragmentation, while sheep grazing is shown to have stronger impacts on the heterogeneity within the montado patches

First record of <i>Banderomys leanzai Kramarz</i>, 2005 (Rodentia, Caviomorpha) in Chubut Province, Patagonia (Argentina)

Cephalomyidae was established in the XIX century, and was considered by different authors as a heterogeneous group of hypsodont South American hystricognath rodents. Other authors corroborated this family as a natural group among caviomorphs and recognized some taxa recorded from the Deseadan-Colhuehuapian South American Land Mammals Ages (SALMAs). Among them, Banderomys leanzai (the only species of this genus) was, until now, characteristic and exclusive of the locality of Cerro Bandera (early Miocene, Colhuehuapian?, Neuquén Province, Argentina). The aim of this work is to report the first record of B. leanzai outside the type locality; the new specimens come from the early Miocene of central Chubut Province (Patagonia, Argentina). This new finding extends the geographical distribution of the species and increases the dental morphology knowledge of B. leanzai.Facultad de Ciencias Naturales y Muse

Urban population looking for rural landscapes: Different appreciation patterns identified in Southern Europe

In the context of the peripheral European rural landscapes, the role of the urban population, generally referred to as the ‘outsiders’, has shown to be influential in the dynamics of rural space. This influence occurs namely through the demand for non-productive functions leading to the emergence of new modes of occupancy. In addition, the emerging policy framework concerning policies and planning in rural landscapes call for an improved understanding of the diversified social demands for these landscapes. We argue that a more profound knowledge on the urban demand for rural landscape is needed to better integrate the urban interests into rural policy and planning. The present paper aims to gain greater insight on this demand by identifying landscape preferences of urban users, framed by the multifunctional transition theory, and using a photo-based survey with contrasting land covers derived from CORINE Land Cover classes. Furthermore, the use of land cover classes as the main landscape component, and thereby relating preferences to specific land covers, offers a sound basis for a territorial approach, able to integrate landscape into rural policy and land use planning practice. A case-study in Southern Portugal was developed at the regional scale and results showed different appreciation patterns for rural landscapes varying from humanised and more naturalised landscapes according to the different functions sought by urban users. Another prominent result is that urban demand for rural landscapes, even if driven by consumption, is strongly influenced by both protection and production values. A deeper knowledge on the interests of urban population can be a step forward for rural communities, land managers, and sectoral policy decision-makers to better define investment strategies in rural-urban partnerships facing the growing urban demand over rural space

Identification of genes involved in reproduction and lipid pathway metabolism in wild and domesticated shrimps

The aims of this study were to identify genes involved in reproduction and lipid pathway metabolism in Penaeus monodon and correlate their expression with reproductive performance. Samples of the hepatopancreas and ovaries were obtained from a previous study of the reproductive performance of wild and domesticated P. monodon broodstock. Total mRNA from the domesticated broodstock was used to create two next generation sequencing cDNA libraries enabling the identification of 11 orthologs of key genes in reproductive and nutritional metabolic pathways in P. monodon. These were identified from the library of de novo assembled contigs, including the description of 6 newly identified genes. Quantitative RT-PCR of these genes in the hepatopancreas prior to spawning showed that the domesticated mature females significantly showed higher expression of the Pm Elovl4, Pm COX and Pm SUMO genes. The ovaries of domesticated females had a significantly decreased expression of the Pm Elovl4 genes. In the ovaries of newly spawned females, a significant correlation was observed between hepatosomatic index and the expression of Pm FABP and also between total lipid content and the expression of Pm CYP4. Although not significant, the highest levels of correlation were found between relative fecundity and Pm CRP and Pm CYP4 expression, and between hatching rate and Pm Nvd and Pm RXR expression. This study reports the discovery of genes involved in lipid synthesis, steroid biosynthesis and reproduction in P. monodon. These results indicate that genes encoding enzymes involved in lipid metabolism pathways might be potential biomarkers to assess reproductive performance.&nbsp

The potential of tailed amplicons for SARS-CoV-2 detection in nucleic acid lateral flow assays

Nucleic Acid Lateral Flow Assays (NALFAs) are a promising solution for the point-of-care detection of viruses like SARS-CoV-2. However, they show some drawbacks, such as the great dependency on the use of antibodies and the need for post-amplification protocols that enable the preparation of amplicons for effective readings, as well as low sensitivity. Here, we developed amplicons of a specific SARS-CoV-2 gene tailed with single-strand DNA (ssDNA) sequences to hybridize with DNA probes immobilized on the NALFA strips, thus overcoming the aforementioned problems. Results have shown that tailed primers have not compromised the amplification efficiency and allowed the correct detection of the amplicons in the lateral flow strip. This approach has presented a limit of detection (LOD) of 25 RNA copies /reaction mix (1 copy/μL) and the test of cross-reactivity with other related viruses has not shown any cross-reactivity. Twenty clinical samples were evaluated by NALFA and simultaneously compared with the gold standard RT-qPCR protocol, originating equal results. Although the number of clinical specimens tested being relatively small, this indicates a sensitivity and specificity both of 100%. In short, an alternative NALFA was successfully implemented, rendering an accurate route for SARS-CoV-2 diagnosis, compatible with low-resource settings.info:eu-repo/semantics/publishedVersio