WebSaúde: programa de extensión tecnológica, emprendimiento e innovación en salud

O WebSaúde: Projeto de Extensão Tecnológica, Empreendedorismo e Inovação em Saúde abrange a extensão universitária e a inovação tecnológica na Faculdade de Odontologia da Universidade Federal de Pelotas (UFPel). O objetivo deste relato de experiência é descrever o projeto e a importância de suas ações no ambiente acadêmico e na comunidade em geral no âmbito de inovação em saúde e empreendedorismo. As atividades de destaque são compostas por um Minicurso de Empreendedorismo e Inovação Tecnológica, a produção de material audiovisual divulgado nas redes sociais do projeto oriundos da participação em eventos de empreendedorismo e inovação e a implementação da Disciplina de Empreendedorismo com parceria do SEBRAE para os cursos de pós-graduação da UFPel. Foi evidenciado que cerca de 600 pessoas participaram diretamente das atividades desenvolvidas pelo projeto sendo a maioria acadêmicos de Instituições de Ensino Superior (IES). Além disso, foi percebido ampliação de iniciativas empreendedoras como criação de empresas juniores e startups pelos participantes do projeto. Dessa forma, conclui-se que as ações do projeto WebSaúde foram de extrema importância visto a necessidade de alargar o debate sobre o empreendedorismo e a inovação tecnológica nas IES e comunidade.   The WebSaúde: Technological Extension, Entrepreneurship, and Innovation in Health Project concerns university extension and technological innovation at the Faculty of Dentistry of the Federal University of Pelotas (UFPel). The purpose of this experience report is to describe the project and the importance of its actions in the academic environment and in the community in general, in the sphere of innovation in health and entrepreneurship. The highlighted activities consisted of a mini-course on Entrepreneurship and Technological Innovation, the production of audiovisual material disseminated on social networks of the project arising from participation in entrepreneurship and innovation events, and the implementation of the Entrepreneurship discipline, in partnership with SEBRAE, for postgraduate courses of UFPel. Around 600 people participated directly in the activities developed by the project, the majority of them being academics from higher education institutions (HEIs). In addition, there was an expansion of entrepreneurial initiatives, such as the creation of junior companies and startups by the project participants. It could be concluded that the actions of the WebSaúde project were highly relevant, given the need to broaden the debate on entrepreneurship and technological innovation in HEIs and the community.WebSaúde es un Proyecto de Extensión Tecnológica, Emprendimiento e Innovación en Salud que abarca extensión universitaria e innovación tecnológica en la Facultad de Odontología de la Universidad Federal de Pelotas (UFPel). El propósito de este relato de experiencia es describir el proyecto y la importancia de sus acciones en el ambiente académico y en la comunidad en general en el ámbito de la innovación en salud y emprendimiento. Las actividades principales están compuestas por un taller de Emprendimiento e Innovación Tecnológica, por la producción de material audiovisual difundido en las redes sociales del proyecto derivado de la participación en eventos de emprendimiento e innovación y la implementación de la Asignatura del Emprendimiento en alianza con SEBRAE para cursos de Posgrado de la UFPel. Se evidenció que alrededor de 600 personas participaron directamente en las actividades desarrolladas por el proyecto, en su mayoría académicos de Instituciones de Educación Superior (IES). Además, los participantes del proyecto notaron una expansión de iniciativas emprendedoras como la creación de empresas junior y startups. Así pues, se concluye que las acciones del proyecto WebSaúde fueron de suma importancia, dada la necesidad de ampliar el debate sobre emprendimiento e innovación tecnológica en las IES y en la comunidad.

Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials

The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods: The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results: Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions: These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic

Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva

OBJECTIVE: The aim of this study was to investigate the coronal microleakage of endodontically treated teeth prepared to receive an intracanal post and teeth with an intracanal post but without a prosthetic crown and exposed to contamination by fresh human saliva. MATERIAL AND METHODS: A mechanical-chemical preparation following the step-back technique was carried out in 35 extracted single-rooted human teeth. The teeth were randomly divided into five groups: G1=root canals instrumented, obturated, and prepared to receive an intracanal post (N=10); G2=root canals with cemented posts but without coronal sealing (N=10); PC1=positive control root canals instrumented and open (N=5); PC2=positive control 2 root canals without instrumentation and open (N=5); and NC=negative control healthy teeth (N=5). The crowns were removed except for the control group of intact teeth. The root canals were obturated and sterilized with cobalt 60 gamma irradiation and were then adapted in an apparatus using a Brain Heart Infusion (BHI) medium and fresh human saliva for contamination. Microbial growth was indicated by the presence of turbidity in the BHI liquid medium. RESULTS: Data were submitted to the Kaplan-Meier Survival Analysis and the Holm-Sidak statistic method, which observed an index of 90% of microleakage in root canals after 24 hours for G1 and 70% of microleakage in samples at the end of 40 days for G2. CONCLUSION: The results show that root canals with an intracanal post but without a prosthetic crown can be recontaminated when exposed to fresh human saliva in a short period

Development and characterization of a novel bulk-fill elastomeric temporary restorative composite

Objectives: This study investigated the physical and mechanical properties, antibacterial effect and biocompatibility of novel elastomeric temporary resin-based filling materials (TFMs) containing zinc methacrylate (ZM). Material and Methods: Experimental TFMs were prepared by mixing the zinc methacrylate with monomer, co-monomer, photoinitiator and fillers. A ZM concentration of 0 (control), 0.5% (Z0.5); 1% (Z1), 2% (Z2), or 5% (ZM5) wt% was added to the TFMs. Fermit-N (F) was used for comparison with the experimental material. Microleakage, water sorption/solubility, degree of conversion, depth of cure, ultimate tensile strength, and hardness were determined and compared. A modified direct contact test (DCT) with Enterococcus faecalis and a Streptococcus mutans' biofilm accumulation assay was carried out to evaluate the antimicrobial effect and cytotoxicity of the assay. Statistical comparisons were performed (α=5%). Results: The results showed that the physical and mechanical properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference and some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values. TFMs with ZM killed E. faecalis only after 1 h. Biofilm development of S. mutans was not affected by the inclusion of ZM in the experimental TFMs. Conclusions: The present findings suggest that the physical, mechanical and biological properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference. However, some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values

Malocclusion and Associated Factors in Early Childhood and Knowledge Level of Mothers from Childcare Groups

Objective: To determine the prevalence of malocclusion and associated factors in children and the level of knowledge of mothers participating in the child care group of a basic health unit. Material and Methods: This is a cross-sectional study conducted in children between 18 and 72 months of a childcare group in Pelotas. Children whose parents or legal guardians signed the informed consent term participated in the study. Data were collected through a questionnaire previously tested and applied to the mothers, and a clinical examination of children's occlusion were performed by examiners after training and calibration. Statistical analysis was descriptive and analytic. In the bivariate analyses, it was used the Pearson, Fisher or Linear Trend Qui-Square tests and, in the multivariate analysis, the Poisson regression. Prevalence ratios and 95% confidence intervals were obtained, and a p-value <0.05 was used. Results: Forty-seven children were included and 41.3% presented malocclusion. The prevalence of malocclusion was associated with a higher number of children in the family (p<0.005) and breastfeeding for more than 12 months (p<0.05). The highest prevalence of open bite was associated with the use of a pacifier and working mothers (p<0.05). Conclusion: Most mothers reported having knowledge about the time and use of pacifiers in childhood. In this group, the prevalence of malocclusion in children was high and associated with behavioral and social factors

effect of the silane concentration on the selected properties of an experimental microfilled composite resin

The aim of present study was evaluate the effect of different percentages of an organo-functionalized silane monomer as adhesion promoter between barium borosilicate glass fillers and (co)monomer blend in experimental dental composite resin. Gamma-methacryloxypropyltrimethoxysilane (γ-MPS) was assessed in an experimental luting cement, at the concentrations of 0, 1, 3, 5, 7 and 10 (wt%). The experimental resin without fillers was used as control group. The flexural strength (FS) and elastic modulus (E) were obtained by mini-flexural test and expressed in MPa and GPa, respectively. Water sorption (WS) and solubility (SL) were determined based on ISO standard 4049:2000. Kruskal–Wallis and Student–Newman–Keuls test were used for comparisons of FS, E and WS. The comparisons of SL means were performed using one-way ANOVA and Tukey's method (α = 5 %). The treatment with 3 % silane revealed statistically higher FS, while the group treated with 1 % silane showed statistically higher E than 3 % silane (p < 0.05) and E similar to control. The experimental composite without filler content showed the highest SL (p < 0.05) while the control composite showed the highest WS (p < 0.05). Based on present findings, flexural strength and elastic modulus can sometimes be improved with lower concentrations (1–3 %) rather than higher concentrations (5–7 %) of the silane (γ-MPS) used as coupling agent on barium borosilicate glass filler microparticles of the dental composite resin

Protocolos empregados em serviços de saúde relacionados a casos pós-exposição ocupacional por material perfuro cortante: uma revisão sistemática / Protocols employed in occupational health services related to post-exposure cases by sharp-hole material: a systematic review

Introdução: as instituições de saúde prestam serviços a fim de atender, tratar e curar pacientes de diversas patologias, por outro lado, é um ambiente que expõe a equipe a uma série de riscos ocupacionais, dentre os quais destacam-se: os acidentes com instrumentais perfurantes cortantes potencialmente contaminados. Objetivo: analisar os protocolos referentes a pós exposição ocupacional a material biológico por perfuro cortante nos serviços de saúde quanto a sua aplicabilidade prática, tipo, formato, o serviço de acolhimento e os métodos de acompanhamento pós exposição. Métodos. Trata-se de uma Scoping Review de artigos sobre condutas pós acidente com material biológico por perfuro cortante, e/ ou sobre Protocolo pós-exposição ocupacional, no período de julho de 2018, conduzida pela The Joanna Brigs Institute for Scoping Review sof Interventions (PRISMA). Resultados: foram capturados nas bases de dados um total de 277 manuscritos. Após triagem de título e resumo, 38 estudos permaneceram, reduzindo-se para 24 após um exame dos textos completos; 16 citam protocolos de referência nacionais e apenas 8 fazem alusão a protocolos institucionais. Esses evidenciaram mais resolutividade nos atendimentos das ocorrências, suporte no acolhimento, acompanhamento e seguimento do evento até o desfecho final. Conclusão: considera-se que a existência de um protocolo institucional é de suma importância, bem como a necessidade do assessoramento destinado a acolher o trabalhador em situação de acidente ocupacional por perfuro cortante

Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells

Macroalgae are a natural source of clinically relevant molecules such as polyunsaturated and monounsaturated fatty acids. The Antarctic environment, due to its cold climate, leads to high production of these bioactive molecules. Adenocystis utricularis, Curdiea racovitzae, and Georgiella confluens from three distinct islands in the Antarctic Peninsula were collected and analyzed for their fatty acid content by gas chromatography flame ionization detection. Results revealed that the algal extracts consisted of 22 fatty acids, of which 9 were saturated, 4 were monounsaturated, and 9 were polyunsaturated (PUFA). In addition, fucosterol was identified within the lipidic extracts. The cytotoxic activity of these fatty acids was evaluated in human breast cancer cell lines MCF-7 and MDA-MB-231. The most notable result was the effect of PUFA on the growth inhibition of cancer cells ranging from 61.04 to 69.78% in comparison to control cells. Significant cytotoxic activity of fatty acids from A. utricularis was observed at 48 h, resulting in an inhibition of growth of more than 50% for breast cancer cells at a concentration of 100 μg/mL. A cell viability assay showed that the fatty acids from A. utricularis significantly reduced cell viability (68.7% in MCF-7 and 89% in MDA-MB-231 after 72 h of exposure). At the same time, DAPI staining demonstrated chromatin condensation, and apoptotic bodies formed in cells that were cultured with fatty acids from A. utricularis. These data indicate that fatty acids from Antarctic macroalgae have the potential to reduce the proliferation of and induce apoptosis in breast cancer cells