Loliolide, a new therapeutic option for neurological diseases? In vitro neuroprotective and anti-inflammatory activities of a monoterpenoid lactone isolated from codium tomentosum

Parkinsons Disease (PD) is the second most common neurodegenerative disease worldwide, and is characterized by a progressive degeneration of dopaminergic neurons. Without an effective treatment, it is crucial to find new therapeutic options to fight the neurodegenerative process, which may arise from marine resources. Accordingly, the goal of the present work was to evaluate the ability of the monoterpenoid lactone Loliolide, isolated from the green seaweed Codium tomentosum, to prevent neurological cell death mediated by the neurotoxin 6-hydroxydopamine (6-OHDA) on SH-SY5Y cells and their anti-inflammatory effects in RAW264.7 macrophages. Loliolide was obtained from the diethyl ether extract, purified through column chromatography and identified by NMR spectroscopy. The neuroprotective effects were evaluated by the MTT method. Cells’ exposure to 6-OHDA in the presence of Loliolide led to an increase of cells’ viability in 40%, and this effect was mediated by mitochondrial protection, reduction of oxidative stress condition and apoptosis, and inhibition of the NF-KB pathway. Additionally, Loliolide also suppressed nitric oxide production and inhibited the production of TNF- and IL-6 pro-inflammatory cytokines. The results suggest that Loliolide can inspire the development of new neuroprotective therapeutic agents and thus, more detailed studies should be considered to validate its pharmacological potential.info:eu-repo/semantics/publishedVersio

Dento-alveolar trauma rehabilitation using fixed prosthesis: clinical case report

Dental trauma is an unexpected, accidental event and requires immediate emergency attention. There are several etiological factors and almost any collision between the face and an object can result in a dental trauma. Objective: The objective of this study is to report a case of dento-alveolar trauma and its dental management, promoting the patient's well-being, restoring health, function and aesthetics. Case report: A 20-year-old male patient came to the dental clinic of CEUNI - FAMETRO complaining of dissatisfaction with his broken tooth after a sports collision. Intraoral clinical examination revealed a large coronary loss below the gingival level in element 15, leaving only the buccal and lingual faces. In the initial radiographic examination, it was verified the presence of an endodontic treatment already performed, but endodontic retreatment was necessary, due to exposure and contamination of the filling material. The treatment plan proposed to the patient consisted of rehabilitation with an intraradicular retainer and then the cementation of a ceramic crown. Conclusion: The study concluded that the rehabilitation with the use of an intraradicular retainer, the fiberglass pin and the metal-free ceramic crown also obtained good results. Thus, it was observed that these materials were able to return the functionality of the tooth, oral health and aesthetics with a natural aspect to the element, the patient reported that he was very satisfied with the final result of his treatment

EXTRATOS HEXÂNICOS DE FUMO, ALHO E PIMENTA-ROXA SOBRE FRANKLINIELLA SCHULTZEI (THYSANOPTERA: THRIPIDAE)

O tomateiro é uma das hortaliças mais importantes, no entanto o ataque de pragas, como o tripes, Frankliniella schultzei, pode causar prejuízos a essa cultura, principalmente por transmitir o complexo de vírus denominados de vira-cabeça do tomateiro. Contudo, os extratos de plantas podem ser uma alternativa no manejo de pragas. Assim, o objetivo foi avaliar o potencial inseticida de extratos hexânicos de fumo, alho e pimenta-roxa sobre F. schultzei. Esses extratos na concentração de 500 µg mL-1 foram testadas sobre ninfas de segundo ínstar de F. schultzei. O extrato hexânico de fumo foi o que causa maior mortalidade (28,1%). Assim, nenhum dos extratos hexânicos avaliados neste trabalho causa mortalidade satisfatória sobre ninfas de F. schultzei, na concentração de 500 µg mL-1

Diagnóstico das ONG em Portugal

info:eu-repo/semantics/publishedVersio

MAMMALS IN PORTUGAL : A data set of terrestrial, volant, and marine mammal occurrences in P ortugal

Mammals are threatened worldwide, with 26% of all species being includedin the IUCN threatened categories. This overall pattern is primarily associatedwith habitat loss or degradation, and human persecution for terrestrial mam-mals, and pollution, open net fishing, climate change, and prey depletion formarine mammals. Mammals play a key role in maintaining ecosystems func-tionality and resilience, and therefore information on their distribution is cru-cial to delineate and support conservation actions. MAMMALS INPORTUGAL is a publicly available data set compiling unpublishedgeoreferenced occurrence records of 92 terrestrial, volant, and marine mam-mals in mainland Portugal and archipelagos of the Azores and Madeira thatincludes 105,026 data entries between 1873 and 2021 (72% of the data occur-ring in 2000 and 2021). The methods used to collect the data were: live obser-vations/captures (43%), sign surveys (35%), camera trapping (16%),bioacoustics surveys (4%) and radiotracking, and inquiries that represent lessthan 1% of the records. The data set includes 13 types of records: (1) burrowsjsoil moundsjtunnel, (2) capture, (3) colony, (4) dead animaljhairjskullsjjaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8),observation in shelters, (9) photo trappingjvideo, (10) predators dietjpelletsjpine cones/nuts, (11) scatjtrackjditch, (12) telemetry and (13) vocalizationjecholocation. The spatial uncertainty of most records ranges between 0 and100 m (76%). Rodentia (n=31,573) has the highest number of records followedby Chiroptera (n=18,857), Carnivora (n=18,594), Lagomorpha (n=17,496),Cetartiodactyla (n=11,568) and Eulipotyphla (n=7008). The data setincludes records of species classified by the IUCN as threatened(e.g.,Oryctolagus cuniculus[n=12,159],Monachus monachus[n=1,512],andLynx pardinus[n=197]). We believe that this data set may stimulate thepublication of other European countries data sets that would certainly contrib-ute to ecology and conservation-related research, and therefore assisting onthe development of more accurate and tailored conservation managementstrategies for each species. There are no copyright restrictions; please cite thisdata paper when the data are used in publications.info:eu-repo/semantics/publishedVersio

Mammals in Portugal: a data set of terrestrial, volant, and marine mammal occurrences in Portugal

Mammals are threatened worldwide, with ~26% of all species being included in the IUCN threatened categories. This overall pattern is primarily associated with habitat loss or degradation, and human persecution for terrestrial mammals, and pollution, open net fishing, climate change, and prey depletion for marine mammals. Mammals play a key role in maintaining ecosystems functionality and resilience, and therefore information on their distribution is crucial to delineate and support conservation actions. MAMMALS IN PORTUGAL is a publicly available data set compiling unpublished georeferenced occurrence records of 92 terrestrial, volant, and marine mammals in mainland Portugal and archipelagos of the Azores and Madeira that includes 105,026 data entries between 1873 and 2021 (72% of the data occurring in 2000 and 2021). The methods used to collect the data were: live observations/captures (43%), sign surveys (35%), camera trapping (16%), bioacoustics surveys (4%) and radiotracking, and inquiries that represent less than 1% of the records. The data set includes 13 types of records: (1) burrows | soil mounds | tunnel, (2) capture, (3) colony, (4) dead animal | hair | skulls | jaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8), observation in shelters, (9) photo trapping | video, (10) predators diet | pellets | pine cones/nuts, (11) scat | track | ditch, (12) telemetry and (13) vocalization | echolocation. The spatial uncertainty of most records ranges between 0 and 100 m (76%). Rodentia (n =31,573) has the highest number of records followed by Chiroptera (n = 18,857), Carnivora (n = 18,594), Lagomorpha (n = 17,496), Cetartiodactyla (n = 11,568) and Eulipotyphla (n = 7008). The data set includes records of species classified by the IUCN as threatened (e.g., Oryctolagus cuniculus [n = 12,159], Monachus monachus [n = 1,512], and Lynx pardinus [n = 197]). We believe that this data set may stimulate the publication of other European countries data sets that would certainly contribute to ecology and conservation-related research, and therefore assisting on the development of more accurate and tailored conservation management strategies for each species. There are no copyright restrictions; please cite this data paper when the data are used in publications

Estudo numérico e experimental da biomecânica do fémur intacto e com prótese de anca inserida : Biomecânica da anca

Disertação apresentada para obtenção do grau de Mestre em Engenharia Biomédica, na Faculdade de Engenharia da Universidade do Porto, sob a orientação do Professor Doutor José António de Oliveira Simõe