Characterisation of Na/K-ATPase, its isoforms, and the inotropic response to ouabain in isolated failing human hearts

Objective: The aim was to determine whether failing human hearts have increased sensitivity to the inotropic and toxic effects of ouabain, and to examine alterations in Na/K-ATPase that might explain the observed higher ouabain sensitivity. Methods: For contractility studies, a total of 57 trabeculae were isolated from two non- failing (death from head injury) and 10 terminally failing, explanted human hearts. After the experiment, each trabecula was inspected under the light microscope for morphological alterations consistent with heart failure. Samples for biochemical and molecular studies were obtained from five non-failing and 13 failing hearts. Total Na/K-ATPase was measured in desoxycholate treated homogenates and expressed per unit of tissue wet or dry weight, DNA, protein, or myosin. Interference from residual bound digoxin due to previous therapy was excluded. The expression of the three α isoforms was studied at both the mRNA level using northern blots and the protein level by analysis of dissociation kinetics of the [3H]ouabain-enzyme complex. Results: Trabeculae showing morphological alterations and decreased contractility were sensitive to lower concentrations of ouabain (3-100 nM) than control trabeculae (100-1000 nM); the inotropic EC50 and the minimum toxic concentration were both reduced. [3H]Ouabain binding was significantly lower (p≪0.001) in failing than in non-failing hearts, at 293(SD 74) v 507(48) pmol·g−1 wet weight. No significant change was observed in maximum ATPase turnover rate, or in sensitivities to Na+, K+, vanadate, and dihydro-ouabain. All three α isoforms were expressed at the mRNA level in both normal and failing hearts. Conclusions: This study shows conclusively, for the first time, that failing human hearts are more sensitive to ouabain. This may be at least partly due to a mean reduction of 42% (95% confidence interval, 26 to 56%) in the concentration of Na/K-ATPase (decrease in Na,K pump reserve), but not to an alteration in its catalytic properties or in its isoform composition. Cardiovascular Research 1993;27:2229-223

Statistical equilibrium of silicon in the solar atmosphere

The statistical equilibrium of neutral and ionised silicon in the solar photosphere is investigated. Line formation is discussed and the solar silicon abundance determined. High-resolution solar spectra were used to determine solar $\log gf\epsilon_{\rm Si}$ values by comparison with Si line synthesis based on LTE and NLTE level populations. The results will be used in a forthcoming paper for differential abundance analyses of metal-poor stars. A detailed analysis of silicon line spectra leads to setting up realistic model atoms, which are exposed to interactions in plane-parallel solar atmospheric models. The resulting departure coefficients are entered into a line-by-line analysis of the visible and near-infrared solar silicon spectrum. The statistical equilibrium of \ion{Si}{i} turns out to depend marginally on bound-free interaction processes, both radiative and collisional. Bound-bound interaction processes do not play a significant role either, except for hydrogen collisions, which have to be chosen adequately for fitting the cores of the near-infrared lines. Except for some near-infrared lines, the NLTE influence on the abundances is weak. Taking the deviations from LTE in silicon into account, it is possible to calculate the ionisation equilibrium from neutral and ionised lines. The solar abundance based on the experimental $f$-values of Garz corrected for the Becker et al.'s measurement is $7.52 \pm 0.05$. Combined with an extended line sample with selected NIST $f$-values, the solar abundance is $7.52 \pm 0.06$, with a nearly perfect ionisation equilibrium of \Delta\log\epsilon_\odot(\ion{Si}{ii}/\ion{Si}{i}) = -0.01.Comment: 13pages 10 figures. A&A acceptte

Anomalous Hopping Exponents of Ultrathin Films of Metals

The temperature dependence of the resistance R(T) of ultrathin quench-condensed films of Ag, Bi, Pb and Pd has been investigated. In the most resistive films, R(T)=Roexp(To/T)^x, where x=0.75. Surprisingly, the exponent x was found to be constant for a wide range of Ro and To in all four materials, possibly implying a consistent underlying conduction mechanism. The results are discussed in terms of several different models of hopping conduction.Comment: 6 pages, 5 figure

An absolutely calibrated effective temperature scale from the InfraRed Flux Method

Various effective temperature scales have been proposed over the years. Despite much work and the high internal precision usually achieved, systematic differences of order 100 K (or more) among various scales are still present. We present an investigation based on the Infrared Flux Method aimed at assessing the source of such discrepancies and pin down their origin. We break the impasse among different scales by using a large set of solar twins, stars which are spectroscopically and photometrically identical to the Sun, to set the absolute zero point of the effective temperature scale to within few degrees. Our newly calibrated, accurate and precise temperature scale applies to dwarfs and subgiants, from super-solar metallicities to the most metal-poor stars currently known. At solar metallicities our results validate spectroscopic effective temperature scales, whereas for [Fe/H]<-2.5 our temperatures are roughly 100 K hotter than those determined from model fits to the Balmer lines and 200 K hotter than those obtained from the excitation equilibrium of Fe lines. Empirical bolometric corrections and useful relations linking photometric indices to effective temperatures and angular diameters have been derived. Our results take full advantage of the high accuracy reached in absolute calibration in recent years and are further validated by interferometric angular diameters and space based spectrophotometry over a wide range of effective temperatures and metallicities.Comment: Accepted for publication in Astronomy and Astrophysics. Landscape table available online at http://www.mpa-garching.mpg.de/~luca/IRFM

Electronic properties and Fermi surface of Ag(111) films deposited onto H-passivated Si(111)-(1x1) surfaces

Silver films were deposited at room temperature onto H-passivated Si(111) surfaces. Their electronic properties have been analyzed by angle-resolved photoelectron spectroscopy. Submonolayer films were semiconducting and the onset of metallization was found at a Ag coverage of $\sim$0.6 monolayers. Two surface states were observed at $\bar{\Gamma}$-point in the metallic films, with binding energies of 0.1 and 0.35 eV. By measurements of photoelectron angular distribution at the Fermi level in these films, a cross-sectional cut of the Fermi surface was obtained. The Fermi vector determined along different symmetry directions and the photoelectron lifetime of states at the Fermi level are quite close to those expected for Ag single crystal. In spite of this concordance, the Fermi surface reflects a sixfold symmetry rather than the threefold symmetry of Ag single crystal. This behavior was attributed to the fact that these Ag films are composed by two domains rotated 60$^o$.Comment: 9 pages, 8 figures, submitted to Physical Review

Knowledge and innovation: The strings between global and local dimensions of sustainable growth

The modern growth literature pays much attention to innovation and knowledge as drivers of endogenous developments in a competitive open economic system. This paper reviews concisely the literature in this field and addresses in particular micro- and macro-economic interactions at local or regional levels, based on clustering and networking principles, in which sustainability conditions also play a core role. The paper then develops a so-called knowledge circuit model comprising the relevant stakeholders, which aims to offer a novel framework for applied policy research at the meso-economic level

Euclid: On the reduced shear approximation and magnification bias for Stage IV cosmic shear experiments

Stage IV weak lensing experiments will offer more than an order of magnitude leap in precision. We must therefore ensure that our analyses remain accurate in this new era. Accordingly, previously ignored systematic effects must be addressed. In this work, we evaluate the impact of the reduced shear approximation and magnification bias, on the information obtained from the angular power spectrum. To first-order, the statistics of reduced shear, a combination of shear and convergence, are taken to be equal to those of shear. However, this approximation can induce a bias in the cosmological parameters that can no longer be neglected. A separate bias arises from the statistics of shear being altered by the preferential selection of galaxies and the dilution of their surface densities, in high-magnification regions. The corrections for these systematic effects take similar forms, allowing them to be treated together. We calculate the impact of neglecting these effects on the cosmological parameters that would be determined from Euclid, using cosmic shear tomography. We also demonstrate how the reduced shear correction can be calculated using a lognormal field forward modelling approach. These effects cause significant biases in Omega_m, n_s, sigma_8, Omega_DE, w_0, and w_a of -0.51 sigma, -0.36 sigma, 0.37 sigma, 1.36 sigma, -0.66 sigma, and 1.21 sigma, respectively. We then show that these lensing biases interact with another systematic: the intrinsic alignment of galaxies. Accordingly, we develop the formalism for an intrinsic alignment-enhanced lensing bias correction. Applying this to Euclid, we find that the additional terms introduced by this correction are sub-dominant