The impact of in-season national team soccer play on injury and player availability in a professional club

This study investigated the impact of in-season national team duty on injury rates and player availability in a professional soccer club. Time-loss injuries and exposure time during club and national team duties were recorded prospectively over 5 seasons (2009–2014). A time-loss injury was sustained by 37.7% of squad members participating in national duty, all injuries occurring in match-play. The incidence (per 1000 h exposure) for national team player match-play injuries did not differ (P = 0.608) to that for all players in club competitions: 48.0 (95% CI 20.9–75.5) vs. 41.9 (95% CI 36.5–47.4), incidence rate ratio = 1.2 (CI: 0.8–2.4). The majority (58%) of national team injuries resulted in a layoff ≤1 week. Of all working days lost to injury generally, 5.2% were lost through injury on national duty. Injury incidence in the week following national duty was comparable (P = 0.818) in players participating or not: 7.8 (95% CI 3.6–12.0) vs. 7.1 (95% CI: 4.6–9.6), incidence rate ratio = 1.1 (CI: 0.7–2.7). While approximately 40% of participating players incurred a time-loss injury on national duty, no training injuries were sustained and injuries made up a negligible part of overall club working days lost to injury. Following duty, players had a similar injury risk to peers without national obligations

Is There Good Simulation Basic Training for End-To-Side Vascular Microanastomoses?

Background: Microvascular anastomosis is the cornerstone of free tissue transfers. Irrespective of the microsurgical technique that one seeks to integrate or improve, the time commitment in the laboratory is significant. After extensive previous training on several animal models, we sought to identify an animal model that circumvents the following issues: ethical rules, cost, time-consuming and expensive anesthesia, and surgical preparation of tissues required to access vessels before performing the microsurgical training, not to mention that laboratories are closed on weekends. Methods: Between January 2012 and April 2012, a total of 91 earthworms were used for 150 microsurgical training exercises to simulate vascular end-to-side microanastomosis. The training sessions were divided into ten periods of 7days. Each training session included 15 simulations of end-to-side vascular microanastomoses: larger than 1.5mm (n=5), between 1.0 and 1.5mm (n=5), and smaller than 1.0mm (n=5). A linear model with the main variables being the number of weeks (as a numerical covariate) and the size of the animal (as a factor) was used to determine the trend in time of anastomosis over subsequent weeks as well as the differences between the different size groups. Results: The linear model shows a significant trend (p<0.001) in time of anastomosis in the course of the training, as well as significant differences (p<0.001) between the groups of animals of different sizes. For microanastomoses larger than 1.5mm, the mean anastomosis time decreased from 19.3±1.0 to 11.1±0.4min between the first and last week of training (decrease of 42.5%). For training with smaller diameters, the results showed a decrease in execution time of 43.2% (diameter between 1.0 and 1.5mm) and 40.9% (diameter <1.0mm) between the first and last periods. The study demonstrates an improvement in the dexterity and speed of nodes execution. Conclusion: The earthworm appears to be a reliable experimental model for microsurgical training of end-to-side microanastomoses. Its numerous advantages are discussed here and we predict training on earthworms will significantly grow and develop in the near future. Level of Evidence III: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/0026

Analysis of repeated high-intensity running performance in professional soccer

The aims of this study conducted in a professional soccer team were two-fold: to characterise repeated high-intensity movement activity profiles in official match-play; b) to inform and verify the construct validity of tests commonly used to determine repeated-sprint ability in soccer by investigating the relationship between the results from a test of repeated-sprint ability and repeated high-intensity performance in competition. High-intensity running performance (movement at velocities >19.8 km/h for a minimum of 1-s duration) in 20 players was measured using computerised time motion analysis. Performance in 80 French League 1 matches was analysed. In addition, 12 out of the 20 players performed a repeated-sprint test on a non-motorized treadmill consisting of 6 consecutive 6s sprints separated by 20s passive recovery intervals. In all players, the majority of consecutive high-intensity actions in competition were performed after recovery durations ≥61s, recovery activity separating these efforts was generally active in nature with the major part of this spent walking, and players performed 1.1±1.1 repeated high-intensity bouts (a minimum of 3 consecutive high-intensity with a mean recovery time ≤20s separating efforts) per game. Players reporting lowest performance decrements in the repeated-sprint ability test performed more high-intensity actions interspersed by short recovery times (≤20s, p<0.01 and ≤30s, p<0.05) compared to those with higher decrements. Across positional roles, central-midfielders performed a greater number of high-intensity actions separated by short recovery times (≤20s) and spent a larger proportion of time running at higher intensities during recovery periods while fullbacks performed the most repeated high-intensity bouts (statistical differences across positional roles from p<0.05 to p<0.001). These findings have implications for repeated high-intensity testing and physical conditioning regimens

Injury risk factors, screening tests and preventative strategies: A systematic review of the evidence that underpins the perceptions and practices of 44 football (soccer) teams from various premier leagues

Purpose: To systematically review the scientific level of evidence for the ‘Top 3’ risk factors, screening tests and preventative exercises identified by a previously published survey of 44 premier league football (soccer) teams. Also, to provide an overall scientific level of evidence and graded recommendation based on the current research literature. Methods: A systematic literature search (Pubmed [MEDLINE], SportDiscus, PEDRO and Cochrane databases). The quality of the articles was assessed and a level of evidence (1++ to 4) was assigned. Level 1++ corresponded to the highest level of evidence available and 4, the lowest. A graded recommendation (A: strong, B: moderate, C: weak, D: insufficient evidence to assign a specific recommendation) for use in the practical setting was given. Results: Fourteen studies were analysed. The overall level of evidence for the risk factors previous injury, fatigue and muscle imbalance were 2++, 4 and ‘inconclusive’, respectively. The graded recommendation for functional movement screen, psychological questionnaire and isokinetic muscle testing were all ‘D’. Hamstring eccentric had a weak graded ‘C’ recommendation, and eccentric exercise for other body parts was ‘D’. Balance/proprioception exercise to reduce ankle and knee sprain injury was assigned a graded recommendation ‘D’. Conclusions: The majority of perceptions and practices of premier league teams have a low level of evidence and low graded recommendation. This does not imply that these perceptions and practices are not important or not valid, as it may simply be that they are yet to be sufficiently validated or refuted by research

Matrix Metalloproteinase-13 Predominates Over Matrix Metalloproteinase-8 as the Functional Interstitial Collagenase in Mouse Atheromata

Objective—Substantial evidence implicates interstitial collagenases of the matrix metalloproteinase (MMP) family in plaque rupture and fatal thrombosis. Understanding the compensatory mechanisms that may influence the expression of these enzymes and their functions, therefore, has important clinical implications. This study assessed in mice the relative effect of the 2 principal mouse collagenases on collagen content and other plaque characteristics. Approach and Results—Apolipoprotein E–deficient (apoE−/−) mice, MMP-13−/− apoE−/−, MMP-8−/− apoE−/− double knockout mice, and MMP-13−/− MMP-8−/− apoE−/− triple knockout mice consumed a high-cholesterol diet for 10 and 24 weeks. Both double knockout and triple knockout mice showed comparable atherosclerotic lesion formation compared with apoE−/− controls. Analysis of aortic root sections indicated that lesions of MMP-8/MMP-13–deficient and MMP-13–deficient mice accumulate more fibrillar collagen than apoE−/− controls and MMP-8−/− apoE−/− double knockout. We further tested the relative effect of MMPs on plaque collagenolysis using in situ zymography. MMP-13 deletion alone abrogated collagenolytic activity in lesions, indicating a predominant role for MMP-13 in this process. MMP-13 and MMP-13/MMP-8 deficiency did not alter macrophage content but associated with reduced accumulation of smooth muscle cells. Conclusions—These results show that among MMP interstitial collagenases in mice, MMP-13 prevails over MMP-8 in collagen degradation in atheromata. These findings provide a rationale for the identification and selective targeting a predominant collagenase for modulating key aspects of plaque structure considered critical in clinical complications, although they do not translate directly to human lesions, which also contain MMP-1

The impact of short periods of match congestion on injury risk and patterns in an elite football club

Background: The effect of fixture congestion on injury rates and patterns has received scarce attention in elite football and existing investigations have not accounted for player rotation or examined the temporal distribution and potential cause of injuries. Aim: To prospectively investigate the epidemiology of injury during short periods of fixture congestion in a professional football club. Methods: Over a 6-season period, exposure time and injury data were compared in the same players (n=25 [14 individuals]) when participating in two frequently occurring short congested fixture cycles in comparison to match-play outside these cycles. 1) two successive matches separated by an interval totalling ≤3days calculated immediately from the end of play in match 1 to the beginning of play in match 2; 2) three successive matches separated by ≤4-day intervals commencing the day immediately after each match. Results: In 2-match congestion cycles, incidence rate ratios (IRR) showed there was a higher risk of injury in the final 15-minutes of play in the second match in comparison to match-play outside the cycles (IRR: 3.1 [95% CI 1.1 to 9.3], p=0.0400). A greater risk of injury overall (IRR: 2.0 [95% CI 1.1 to 3.8], p=0.0345) and in the 1st-half of play (2.6 [1.1 to 6,5], p=0.0386), and risk of ankle sprains (10.4 [95% CI 1.9 to 57.9], p=0.0068) and non-contact injuries due to a ‘change in direction’ (IRR: 7.8 [1.3 to 46.8], p=0.0243) was observed in the final match of 3-match congestion cycles in comparison to match-play outside the cycles. Conclusion: Injury rates and patterns were affected in the same elite football players when competing in short congested fixture cycles in comparison to match-play outside the cycles

Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009

[en] Background: Molecular hydrogen, given its pollution-free combustion, has great potential to replace fossil fuels infuture transportation and energy production. However, current industrial hydrogen production processes, such assteam reforming of methane, contribute significantly to the greenhouse effect. Therefore alternative methods, inparticular the use of fermentative microorganisms, have attracted scientific interest in recent years. However thelow overall yield obtained is a major challenge in biological H2 production. Thus, a thorough and detailedunderstanding of the relationships between genome content, gene expression patterns, pathway utilisation andmetabolite synthesis is required to optimise the yield of biohydrogen production pathways.Results: In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridiumbutyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when themetabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitoredin 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differenceswere observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and theywere pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated withconcurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation ofthe [FeFe] hydrogenase genes was observed. During the fixed pH 5.2 fermentation, by contrast, the expressionlevels for the [FeFe] hydrogenase coding genes were higher than during the unregulated-pH fermentation, whilethe nitrogenase transcripts were less abundant. The overall results suggest, for the first time, that environmentalfactors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/orthe nitrogenase.Conclusions: This work, contributing to the field of dark fermentative hydrogen production, provides amultidisciplinary approach for the investigation of the processes involved in the molecular H2 metabolism ofclostridia. In addition, it lays the groundwork for further optimisation of biohydrogen production pathways basedon genetic engineering techniques.info:eu-repo/semantics/publishe