514 research outputs found
Deformation of grain boundaries in polar ice
The ice microstructure (grain boundaries) is a key feature used to study ice
evolution and to investigate past climatic changes. We studied a deep ice core,
in Dome Concordia, Antarctica, which records past mechanical deformations. We
measured a "texture tensor" which characterizes the pattern geometry and
reveals local heterogeneities of deformation along the core. These results
question key assumptions of the current models used for dating
Growth laws and self-similar growth regimes of coarsening two-dimensional foams: Transition from dry to wet limits
We study the topology and geometry of two dimensional coarsening foams with
arbitrary liquid fraction. To interpolate between the dry limit described by
von Neumann's law, and the wet limit described by Marqusee equation, the
relevant bubble characteristics are the Plateau border radius and a new
variable, the effective number of sides. We propose an equation for the
individual bubble growth rate as the weighted sum of the growth through
bubble-bubble interfaces and through bubble-Plateau borders interfaces. The
resulting prediction is successfully tested, without adjustable parameter,
using extensive bidimensional Potts model simulations. Simulations also show
that a selfsimilar growth regime is observed at any liquid fraction and
determine how the average size growth exponent, side number distribution and
relative size distribution interpolate between the extreme limits. Applications
include concentrated emulsions, grains in polycrystals and other domains with
coarsening driven by curvature
Isolation and primary cultures of human intrahepatic bile ductular epithelium
A technique for the isolation of human intrahepatic bile ductular epithelium, and the establishment of primary cultures using a serum- and growth-factor-supplemented medium combined with a connective tissue substrata is described. Initial cell isolates and monolayer cultures display phenotypic characteristics of biliary epithelial cells (low molecular weight prekeratin positive; albumin, alphafetoprotein, and Factor VIII-related antigen negative). Ultrastructural features of the cultured cells show cell polarization with surface microvilli, numerous interepithelial junctional complexes and cytoplasmic intermediate prekeratin filaments. © 1988 Tissue Culture Association, Inc
Two-dimensional flow of foam around an obstacle: force measurements
A Stokes experiment for foams is proposed. It consists in a two-dimensional
flow of a foam, confined between a water subphase and a top plate, around a
fixed circular obstacle. We present systematic measurements of the drag exerted
by the flowing foam on the obstacle, \emph{versus} various separately
controlled parameters: flow rate, bubble volume, bulk viscosity, obstacle size,
shape and boundary conditions. We separate the drag into two contributions, an
elastic one (yield drag) at vanishing flow rate, and a fluid one (viscous
coefficient) increasing with flow rate. We quantify the influence of each
control parameter on the drag. The results exhibit in particular a power-law
dependence of the drag as a function of the bulk viscosity and the flow rate
with two different exponents. Moreover, we show that the drag decreases with
bubble size, and increases proportionally to the obstacle size. We quantify the
effect of shape through a dimensioned drag coefficient, and we show that the
effect of boundary conditions is small.Comment: 26 pages, 13 figures, resubmitted version to Phys. Rev.
A quantum-like description of the planetary systems
The Titius-Bode law for planetary distances is reviewed. A model describing
the basic features of this rule in the "quantum-like" language of a wave
equation is proposed. Some considerations about the 't Hooft idea on the
quantum behaviour of deterministic systems with dissipation are discussed.Comment: LaTex file, 17 pages, no figures. Version published in Foundations of
Physics, August 200
Mechanical Stress Inference for Two Dimensional Cell Arrays
Many morphogenetic processes involve mechanical rearrangement of epithelial
tissues that is driven by precisely regulated cytoskeletal forces and cell
adhesion. The mechanical state of the cell and intercellular adhesion are not
only the targets of regulation, but are themselves likely signals that
coordinate developmental process. Yet, because it is difficult to directly
measure mechanical stress {\it in vivo} on sub-cellular scale, little is
understood about the role of mechanics of development. Here we present an
alternative approach which takes advantage of the recent progress in live
imaging of morphogenetic processes and uses computational analysis of high
resolution images of epithelial tissues to infer relative magnitude of forces
acting within and between cells. We model intracellular stress in terms of bulk
pressure and interfacial tension, allowing these parameters to vary from cell
to cell and from interface to interface. Assuming that epithelial cell layers
are close to mechanical equilibrium, we use the observed geometry of the two
dimensional cell array to infer interfacial tensions and intracellular
pressures. Here we present the mathematical formulation of the proposed
Mechanical Inverse method and apply it to the analysis of epithelial cell
layers observed at the onset of ventral furrow formation in the {\it
Drosophila} embryo and in the process of hair-cell determination in the avian
cochlea. The analysis reveals mechanical anisotropy in the former process and
mechanical heterogeneity, correlated with cell differentiation, in the latter
process. The method opens a way for quantitative and detailed experimental
tests of models of cell and tissue mechanics
A multiscale hybrid model for pro-angiogenic calcium signals in a vascular endothelial cell
Cytosolic calcium machinery is one of the principal signaling mechanisms by which endothelial cells (ECs) respond to external stimuli during several biological processes, including vascular progression in both physiological and pathological conditions. Low concentrations of angiogenic factors (such as VEGF) activate in fact complex pathways involving, among others, second messengers arachidonic acid (AA) and nitric oxide (NO), which in turn control the activity of plasma membrane calcium channels. The subsequent increase in the intracellular level of the ion regulates fundamental biophysical properties of ECs (such as elasticity, intrinsic motility, and chemical strength), enhancing their migratory capacity. Previously, a number of continuous models have represented cytosolic calcium dynamics, while EC migration in angiogenesis has been separately approached with discrete, lattice-based techniques. These two components are here integrated and interfaced to provide a multiscale and hybrid Cellular Potts Model (CPM), where the phenomenology of a motile EC is realistically mediated by its calcium-dependent subcellular events. The model, based on a realistic 3-D cell morphology with a nuclear and a cytosolic region, is set with known biochemical and electrophysiological data. In particular, the resulting simulations are able to reproduce and describe the polarization process, typical of stimulated vascular cells, in various experimental conditions.Moreover, by analyzing the mutual interactions between multilevel biochemical and biomechanical aspects, our study investigates ways to inhibit cell migration: such strategies have in fact the potential to result in pharmacological interventions useful to disrupt malignant vascular progressio
Estudos sobre a alimentação mineral do cafeeiro: XIX. extração de micronutrientes na colheita pelas variedades "Mundo novo", "Caturra Amarelo" e "Bourbon Amarelo"
The contents of micronutrientes in coffee berries of the varieties ""Bourbon Amarelo", "Caturra Amarelo" and "Mundo Novo" grown in three different types of soils, namely, "terra roxa", "massape-salmourão", and "arenito de Bauru", have been determined. Statiscal analysis of the chemical data permits the following conclusions to be drawn: (a) there is no difference in the chemical composition of the three varieties; (b) the average content is as follows (in ppm): (c) one bag of dry, unprocessed coffe, has roughly equal total amounts of micronutrients both in the beans and in the hulls; zinc is an exception, however.Foi determinado o teor de micronutrientes na casca (polpa) e no grão de café "cereja" das variedades "Caturra Amarelo", "Bourbon Amarelo" e "Mundo Novo" colhidos em plantas cultivadas em três tipos de solos - terra roxa legítima, massapé-salmourão e arenito de Bauru. A análise revelou que as três variedades possuem a mesma composição mineral com respeito aos elementos determinados. O tipo de solo - ou o local - influiu na composição das amostras. Em um saco de café em coco existem quantidades aproximadamente iguais dos elementos na casca e no grão
Towards whole genome association genetic scans in barley
In crop plants, the potential of association mapping, with the objective of estimating the position of genes conferring a specific trait or phenotype using linkage disequilibrium (LD) between alleles of genetically mapped markers, has recently become a focus of considerable interest. One major attraction of association genetics is the potential to locate genes responsible for a wide range of traits in a single sample population using pre-existing phenotypic data that has been collected during crop improvement and cultivar registration programs. This study testify to the potential of exploiting whole genome LD-scans to locate genes controlling key biological traits in cultivated barley. We are currently increasing the density of markers, particularly those with a MAF >0.1, by developing two further pilot OPAs, which in due course will be compressed into two commercially available platforms for high throughput low cost genotyping in cultivated barley. In the immediate future these will be used in large association genetic studies in the UK and US involving approximately 4000 barley genotypes with the aim of realising the potential for whole genome association genetic scans in cultivated barley
Genetic dissection of photoperiod response based on GWAS of pre-anthesis phase duration in spring barley
Heading time is a complex trait, and natural variation in photoperiod responses is a major factor controlling time to heading, adaptation and grain yield. In barley, previous heading time studies have been mainly conducted under field conditions to measure total days to heading. We followed a novel approach and studied the natural variation of time to heading in a world-wide spring barley collection (218 accessions), comprising of 95 photoperiod-sensitive (Ppd-H1) and 123 accessions with reduced photoperiod sensitivity (ppd-H1) to long-day (LD) through dissecting pre-anthesis development into four major stages and sub-phases. The study was conducted under greenhouse (GH) conditions (LD; 16/8 h; ∼20/∼16°C day/night). Genotyping was performed using a genome-wide high density 9K single nucleotide polymorphisms (SNPs) chip which assayed 7842 SNPs. We used the barley physical map to identify candidate genes underlying genome-wide association scans (GWAS). GWAS for pre-anthesis stages/sub-phases in each photoperiod group provided great power for partitioning genetic effects on floral initiation and heading time. In addition to major genes known to regulate heading time under field conditions, several novel QTL with medium to high effects, including new QTL having major effects on developmental stages/sub-phases were found to be associated in this study. For example, highly associated SNPs tagged the physical regions around HvCO1 (barley CONSTANS1) and BFL (BARLEY FLORICAULA/LEAFY) genes. Based upon our GWAS analysis, we propose a new genetic network model for each photoperiod group, which includes several newly identified genes, such as several HvCO-like genes, belonging to different heading time pathways in barley
- …