Final report of FORGE WP3.1.1: the large scale gas injection test (Lasgit) performed at the Äspö Hard Rock Laboratory

This report summarises the set-up, operation and observations from the first 2890 days (7.9 years) of the large scale gas injection test (Lasgit) experiment conducted at the Äspö Hard Rock Laboratory. During this time the bentonite buffer has been artificially hydrated and has given new insight into the evolution of the buffer. Three gas injection tests have been conducted during the duration of Lasgit. The first two tests were conducted in the lower array of injection filters at FL903. Both of these tests showed similar behaviour with a well-defined pressure peak; spontaneous negative transient; evidence of dynamic behaviour and unstable gas pathways; asymptote close to stress. The results were remarkably qualitatively similar to the laboratory test results. However, the high gas entry pressures seen in the laboratory were not seen in Lasgit as stress state is much lower due to non-complete hydration of the buffer and the expansion of the buffer to fill construction voids. The third gas test was conducted in an upper array filter (FU910). The response at the time of gas peak pressure was subtly dissimilar to that seen at FL903 with two peak pressures. Lasgit has confirmed the coupling between gas, stress and pore-water pressure for flow before and after major gas entry at the field scale. All observations suggest mechanisms of pathway propagation and dilatancy predominate. In all three gas tests the propagation was through localised features and the general movement direction was towards the bottom of the deposition hole in the direction of the prevailing stress gradient. The injection tests have shown that the interface between barriers is a key part of the system. Gas appears to have exited the deposition hole in Gas test 2, but failed to find a way out during Gas test 3; where gas continued to migrate along the canister/buffer interface. Throughout the history of Lasgit parts of the system have been artificially and naturally hydrated. Hydraulic results, from controlled and uncontrolled events, show that the buffer continues to mature and has yet to reach full maturation. Hydration of the clay is progressing well but sections of bentonite remain in suction and in hydraulic disequilibrium

Basic and ultrabasic volcanic rocks from the Argyll Group (Dalradian) of NE Scotland

The Dalradian Supergroup of the Scottish Highlands is a largely metasedimentary succession of Neoproterozoic to Early Cambrian age, metamorphosed during the Caledonian Orogeny. The rocks were deposited on the Laurentian margin during and following the break-up of Rodinia. This rift setting is evidenced, in the upper half of the succession, by the presence of several volcanic sequences. A significant development of these volcanic rocks occurs in the NE Grampian Highlands. There, the Blackwater Formation, within the Argyll Group of the Huntly area, is dominated by basic and ultrabasic metavolcanic rocks, with intercalated metasedimentary rocks. The rocks were metamorphosed in the amphibolite facies, with the Mg contents of their dominant amphiboles apparently reflecting those of the whole-rock protoliths. The protoliths ranged from ultrabasic picritic types (MgO up to 35 wt%) through high-magnesia basalts to basaltic andesites and andesites. The magmas feeding the volcanism were of tholeiitic affinity, broadly similar to metavolcanic rocks elsewhere in the Dalradian. Higher-Nb and lower-Nb groups can be distinguished. The inferred parental magmas were basalts with MgO c. 10 wt%. The ultrabasic rocks formed by accumulation of olivine and minor Cr-spinel within the parental basalts. Some more evolved rocks show evidence of minor (5–10 wt%) accumulation of Fe–Ti oxides. Primary magmas of the Blackwater metavolcanic rocks were generated from a mantle source, or sources, relatively enriched compared to the MORB source. It is speculated that all the Dalradian metavolcanic rocks represent varying degrees of mixing of magmas from this source and a depleted, MORB-like, mantle source

Evidence of localised gas propagation pathways in a field-scale bentonite engineered barrier system: results from three gas injection tests in the large scale gas injection test (Lasgit)

Three gas injection tests have been conducted during a large scale gas injection test (Lasgit) performed at the Äspö Hard Rock Laboratory, Sweden. Lasgit is a full-scale experiment based on the Swedish KBS-3 repository concept, examining the processes controlling gas and water flow in highly water-saturated compact buffer bentonite. Three preliminary gas injection tests have been performed. The first two tests were conducted in the lower array of injection filters (FL903). Both of these tests showed similar behaviour that corresponded with laboratory observations. The third gas test was conducted in an upper array filter (FU910), which gave a subtly dissimilar response at major gas entry with an initial pressure drop followed by a secondary gas peak pressure. Lasgit has confirmed the coupling between gas, stress and pore-water pressure for flow before and after major gas entry at the field scale. All observations suggest mechanisms of pathway propagation and dilatancy predominate. In all three gas tests the propagation was through localised features that tended to exploit the interface between the copper canister and the bentonite buffer. Considerable evidence exists for the development of a highly-dynamic, tortuous network of pressure induced pathways which evolves both temporally and geospatially within the clay, opening and closing probably due to local changes in gas pressure and or effective stress

Small angle neutron scattering observation of chain retraction after a large step deformation

The process of retraction in entangled linear chains after a fast nonlinear stretch was detected from time-resolved but quenched small angle neutron scattering (SANS) experiments on long, well-entangled polyisoprene chains. The statically obtained SANS data cover the relevant time regime for retraction, and they provide a direct, microscopic verification of this nonlinear process as predicted by the tube model. Clear, quantitative agreement is found with recent theories of contour length fluctuations and convective constraint release, using parameters obtained mainly from linear rheology. The theory captures the full range of scattering vectors once the crossover to fluctuations on length scales below the tube diameter is accounted for

Strategic priorities for respiratory syncytial virus (RSV) vaccine development

AbstractAlthough RSV has been a high priority for vaccine development, efforts to develop a safe and effective vaccine have yet to lead to a licensed product. Clinical and epidemiologic features of RSV disease suggest there are at least 4 distinct target populations for vaccines, the RSV naïve young infant, the RSV naïve child ≥6 months of age, pregnant women (to provide passive protection to newborns), and the elderly. These target populations raise different safety and efficacy concerns and may require different vaccination strategies. The highest priority target population is the RSV naïve child. The occurrence of serious adverse events associated with the first vaccine candidate for young children, formalin inactivated RSV (FI-RSV), has focused vaccine development for the young RSV naïve child on live virus vaccines. Enhanced disease is not a concern for persons previously primed by a live virus infection. A variety of live-attenuated viruses have been developed with none yet achieving licensure. New live-attenuated RSV vaccines are being developed and evaluated that maybe sufficiently safe and efficacious to move to licensure. A variety of subunit vaccines are being developed and evaluated primarily for adults in whom enhanced disease is not a concern. An attenuated parainfluenza virus 3 vector expressing the RSV F protein was evaluated in RSV naïve children. Most of these candidate vaccines have used the RSV F protein in various vaccine platforms including virus-like particles, nanoparticles, formulated with adjuvants, and expressed by DNA or virus vectors. The other surface glycoprotein, the G protein, has also been used in candidate vaccines.We now have tools to make and evaluate a wide range of promising vaccines. Costly clinical trials in the target population are needed to evaluate and select candidate vaccines for advancement to efficacy trials. Better data on RSV-associated mortality in developing countries, better estimates of the risk of long term sequelae such as wheezing after infection, better measures of protection in target populations, and data on the costs and benefits of vaccines for target populations are needed to support and justify funding this process. Addressing these challenges and needs should improve the efficiency and speed of achieving a safe and effective, licensed RSV vaccine

Predicting seawater intrusion in coastal groundwater boreholes using self-potential data

Many coastal groundwater abstraction wells are under threat from seawater intrusion: this is exacerbated in summer by low water tables and increased abstraction. Existing hydrochemistry or geophysical techniques often fail to predict the timing of intrusion events. We investigate whether the presence and transport of seawater can influence self-potentials (SPs) measured within groundwater boreholes, with the aim of using SP monitoring to provide early warning of saline intrusion. SP data collection: SP data were collected from a coastal groundwater borehole and an inland borehole (> 60 km from the coast) in the Seaford Chalk of southern England. The SP gradient in the inland borehole was approximately 0.05 mV/m, while that in the coastal borehole varied from 0.16-0.26 mV/m throughout the monitoring period. Spectral analysis showed that semi-diurnal fluctuations in the SP gradient were several orders of magnitude higher at the coast than inland, indicating a strong influence from oceanic tides. A characteristic decrease in the gradient, or precursor, was observed in the coastal borehole several days prior to seawater intrusion. Modelling results: Hydrodynamic transport and geoelectric modelling suggest that observed pressure changes (associated with the streaming potential) are insufficient to explain either the magnitude of the coastal SP gradient or the semi-diurnal SP fluctuations. By contrast, a model of the exclusion-diffusion potential closely matches these observations and produces a precursor similar to that observed in the field. Sensitivity analysis suggests that both a sharp saline front and spatial variations in the exclusion efficiency arising from aquifer heterogeneities are necessary to explain the SP gradient observed in the coastal borehole. The presence of the precursor in the model depends also on the presence and depth of fractures near the base of the borehole. Conclusions: Our results indicate that SP monitoring, combined with hydrodynamic transport and geoelectric modelling, holds considerable promise as an early warning device for seawater intrusion. We now aim to refine our understanding of the technique by applying it to a range of aquifer types

Evaluation of a culture-based pathogen identification kit for bacterial causes of bovine mastitis

Accurate identification of mastitis-causing bacteria supports effective management and can be used to implement selective use of antimicrobials for treatment. The objectives of this study were to compare the results from a culture-based mastitis pathogen detection test kit (‘VetoRapid’, Vétoquinol) with standard laboratory culture and to evaluate the potential suitability of the test kit to inform a selective treatment programme. Overall 231 quarter milk samples from five UK dairy farms were collected. The sensitivity and specificity of the test kit for the identification of Escherichia coli, Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus uberis and Enterococcus spp. ranged from 17 per cent to 84 per cent and 92 per cent to 98 per cent, respectively. In total, 23 of 68 clinical samples were assigned as meeting the requirement for antimicrobial treatment (Gram-positive organism cultured) according to standard culture results, with the test kit results having sensitivity and specificity of 91 per cent and 78 per cent, respectively. Several occurrences of misidentification are reported, including S. aureus being misidentified as coagulase-negative staphylococci and vice versa. The test kit provides rapid preliminary identification of five common causes of bovine mastitis under UK field conditions and is likely to be suitable for informing selective treatment of clinical mastitis caused by Gram-positive organisms

The potential of small unmanned aircraft systems and structure-from-motion for topographic surveys: a test of emerging integrated approaches at Cwm Idwal, North Wales

This paper was accepted for publication in the journal Geomorphology and the definitive published version is available at http://dx.doi.org/10.1016/j.geomorph.2014.07.021Novel topographic survey methods that integrate both structure-from-motion (SfM) photogrammetry and small unmanned aircraft systems (sUAS) are a rapidly evolving investigative technique. Due to the diverse range of survey configurations available and the infancy of these new methods, further research is required. Here, the accuracy, precision and potential applications of this approach are investigated. A total of 543 images of the Cwm Idwal moraine–mound complex were captured from a light (b5 kg) semi-autonomous multi-rotor unmanned aircraft system using a consumer-grade 18 MP compact digital camera. The imageswere used to produce a DSM(digital surfacemodel) of themoraines. The DSMis in good agreement with 7761 total station survey points providing a total verticalRMSE value of 0.517mand verticalRMSE values as lowas 0.200mfor less densely vegetated areas of the DSM. High-precision topographic data can be acquired rapidly using this technique with the resulting DSMs and orthorectified aerial imagery at sub-decimetre resolutions. Positional errors on the total station dataset, vegetation and steep terrain are identified as the causes of vertical disagreement. Whilst this aerial survey approach is advocated for use in a range of geomorphological settings, care must be taken to ensure that adequate ground control is applied to give a high degree of accuracy

Stellar populations of classical and pseudo-bulges for a sample of isolated spiral galaxies

In this paper we present the stellar population synthesis results for a sample of 75 bulges in isolated spiral Sb-Sc galaxies, using the spectroscopic data from the Sloan Digital Sky Survey and the STARLIGHT code. We find that both pseudo-bulges and classical bulges in our sample are predominantly composed of old stellar populations, with mean mass-weighted stellar age around 10 Gyr. While the stellar population of pseudo-bulges is, in general, younger than that of classical bulges, the difference is not significant, which indicates that it is hard to distinguish pseudo-bulges from classical bulges, at least for these isolated galaxies, only based on their stellar populations. Pseudo-bulges have star formation activities with relatively longer timescale than classical bulges, indicating that secular evolution is more important in this kind of systems. Our results also show that pseudo-bulges have a lower stellar velocity dispersion than their classical counterparts, which suggests that classical bulges are more dispersion-supported than pseudo-bulges.Comment: 10 pages, 8 figures. Accepted for publication in Astrophysics & Space Scienc