Incidence of grapevine leafroll associated viruses -1, -2, and -3 in Mendoza vineyards

Indexación: ScieloViticulture is important in Argentina's economy, especially in the province of Mendoza, which is responsible for more than 75% of the crop cultivated area. In this work, we evaluated the incidence of Grapevine leafroll-associated viruses (GLRaV) -1, -2, and -3 in Vitis vinifera clones of cultivars Cabernet Sauvignon, Cabernet Franc, and Sauvignon Blanc, planted in different zones of Mendoza. The selected clones were previously reported as putatively infected by GLRaV-2. All selected samples were analyzed by DAS-ELISA for GLRaV-1,-2 and -3. GLRaV-2 was the only virus identified in all the analyzed clones. The overall infection rates were 0.6%, 18.8% and 1.2 % for GLRaV-1, 2 and 3 respectively. For the clone Cabernet Sauvignon 337, the infection rate was very high (68.3%).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600007&nrm=is

First description of Grapevine leafroll-associated virus 5 in Argentina and partial genome sequence

An accession of Vitis vinifera cv. Red Globe from Argentina, was found to be infected with Grapevine leafroll-associated virus-5 by ELISA. It was partially sequenced, and three ORFs, corresponding to HSP70h, HSP90h, and CP, were found. This isolate shares a high aminoacid identity with the previously reported sequence of the virus, and identities between 80% and 90% with previously reported GLRaV-9 and GLRaV-4 isolates. The analysis of the sequence supports the clustering together with GLRaV-4 and GLRV-9 inside the Ampelovirus genus.Instituto de Biotecnología y Biología Molecula

Relevamiento de Thysanoptera en cultivos horticolas, como vectores potenciales de Tospovirus en Mendoza, Argentina

Tospoviruses severely affect tomato, lettuce and other crops in Argentina' and are thrips transmitted in nature. The epidemiology of tospoviruses is closely related with vector's ecology. The aim of this study was to know the Thysanoptera fauna in and around the tomato ecosystem of the Mendoza province" and to find rearingmethods for the more common species. Thrips were collected frorn 51 host plants and 19 species were identified, five of which [Bregmatothrips venustus Hood, Chirothrips frontalis Williams, C. texanus (Andre), Frankliniella frumenti Moulton, and F. valdiviana Sakimura &O'Neill] are new records for Mendoza and Karnyothrips longiceps (Hood) for Argentina. Three out of the eight species reported to transmit tospoviruses are present in Mendoza: F. schultzei (Trybom). F. occidentalis (Pergande) and Thrips tabaci Lindeman. Rearing rnethods were developed for eight species: Aptinothrips rufus (Haliday), F. australis Morganl F. frumenti, F. gemina (Bagnall), F. occidentelis, F. schultzei, Haplothrips trellesi Moulton, and T. tabaci. Colonies were reared from a single female in a growth room at 25° (± 2) and L 12: 0 12.En la Argentina el tomate, la lechuga y otras hortalizas, son severamente atacadas por virus del genero Tospovirus. Estos virus, son transmitidos por trips en condiciones naturales. La epidemiología de los tospovirus, esta estrechamente relacionada con la ecología de los vectores. El propósito de estetrabajo, fue conocer la fauna Thysanoptera en el ecosistema de tomate y su area circundante, en la provincia de Mendoza, y tambien poner a punto metodos de cría para las especies mas abundantes. Se coleccionaron trips en 51 huespedes y se identificaron 19 especies de tisanópteros, cinco de los cuales [Bregmatothrips venustus Hood, Chirothrips frontalis Williams, C. texanus (Andre), Frankliniella frumenti Moulton y F. valdiviana Sakimura & 0' Neill] son citas nuevas para Mendoza, y una Karnyothrips longiceps(Hood) para la Argentina. Ocho especies han sido registradas en la bibliografia mundial como vectoras de tospovirus, de las cuales sólo tres estan presentes en Mendoza: F. schultzei (Tryborn), F: occidentalis (Pergande) y Thrips tabaciLindeman. Seajustaron metodos de cría para ocho de las especies identificadas: Aptinothrips rufus(Haliday), F. australisMorgan, F.frumenti, F. gemina (Bagnall), F. occidentalis, F. schultzei, Haplothrips trellesi Moulton y T. tabaci. Las colonias se originaron en una sola hernbra, en carnara de crecimiento a 25°C (± 2) con fotoperíodo de 12 horas.Facultad de Ciencias Naturales y Muse

Incidence of Grapevine Leafroll Associated Viruses -1, -2, and -3 in Mendoza vineyards

Viticulture is important in Argentina's economy, especially in the province of Mendoza, which is responsible for more than 75% of the crop cultivated area. In this work, we evaluated the incidence of Grapevine leafroll-associated viruses (GLRaV) -1, -2, and -3 in Vitis vinifera clones of cultivars Cabernet Sauvignon, Cabernet Franc, and Sauvignon Blanc, planted in different zones of Mendoza. The selected clones were previously reported as putatively infected by GLRaV-2. All selected samples were analyzed by DAS-ELISA for GLRaV-1,-2 and -3. GLRaV-2 was the only virus identified in all the analyzed clones. The overall infection rates were 0.6%, 18.8% and 1.2 % for GLRaV-1, 2 and 3 respectively. For the clone Cabernet Sauvignon 337, the infection rate was very high (68.3%).A viticultura é importante para a economia da Argentina, especialmente na província de Mendoza, que abrange mais de 75% da área cultivada do país. Neste trabalho, nós avaliamos a incidência de Grapevine leafroll associated virus (GLRaV) -1, -2 e -3 em clones de Vitis vinifera das cultivares Cabernet Sauvignon, Cabernet Franc e Sauvignon Blanc, cultivadas em diferentes zonas de Mendoza. Os clones selecionados foram previamente relatados como provavelmente infectados por GLRaV-2. Todas as amostras selecionadas foram analisadas por DAS-ELISA para GLRaV-1, -2 e -3. GLRaV-2 foi o único vírus identificado em todos os clones analisados. As incidëncias das infecçoes globais foram 0,6%, 18,8% e 1,2% para GLRaV-1, 2 e 3, respectivamente. No Cabernet Sauvignon clone 337 a incidëncia da infecção foi muito elevado (68,3%)Fil: Lanza Volpe, Melisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Engel, Esteban A. Fundación Ciencia para la Vida; Chile. Universidad Andrés Bello. Facultad de Ciencias de la Salud; ChileFil: Gracia, Olga. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentin

Incidência do Grapevine Leafroll Associated Viruses -1, -2, e -3 em vinhedos de Mendoza

Indexación: Web of Science; ScieloViticulture is important in Argentina's economy, especially in the province of Mendoza, which is responsible for more than 75% of the crop cultivated area. In this work, we evaluated the incidence of Grapevine leafroll-associated viruses (GLRaV) -1, -2, and -3 in Vitis vinifera clones of cultivars Cabernet Sauvignon, Cabernet Franc, and Sauvignon Blanc, planted in different zones of Mendoza. The selected clones were previously reported as putatively infected by GLRaV-2. All selected samples were analyzed by DAS-ELISA for GLRaV-1,-2 and -3. GLRaV-2 was the only virus identified in all the analyzed clones. The overall infection rates were 0.6%, 18.8% and 1.2 % for GLRaV-1, 2 and 3 respectively. For the clone Cabernet Sauvignon 337, the infection rate was very high (68.3%).A viticultura é importante para a economia da Argentina, especialmente na província de Mendoza, que abrange mais de 75% da área cultivada do país. Neste trabalho, nós avaliamos a incidência de Grapevine leafroll associated virus (GLRaV) -1, -2 e -3 em clones de Vitis vinifera das cultivares Cabernet Sauvignon, Cabernet Franc e Sauvignon Blanc, cultivadas em diferentes zonas de Mendoza. Os clones selecionados foram previamente relatados como provavelmente infectados por GLRaV-2. Todas as amostras selecionadas foram analisadas por DAS-ELISA para GLRaV-1, -2 e -3. GLRaV-2 foi o único vírus identificado em todos os clones analisados. As incidëncias das infecçoes globais foram 0,6%, 18,8% e 1,2% para GLRaV-1, 2 e 3, respectivamente. No Cabernet Sauvignon clone 337 a incidëncia da infecção foi muito elevado (68,3%).http://ref.scielo.org/4br9s

Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene

This research studied serological relationships and genetic diversity of Argentinean isolates of grapevine leafroll-associated virus-4 (GLRaV-4). Phylogenetic analysis of coat protein (CP) sequences from 19 local isolates revealed clustering with the previously described GLRaV-4 strain 5, strain 6, and strain 9 groups. Evolutionary sequence analysis of the obtained and database-available sequences showed evidence of recombination events. Additionally, both CP N- and C-terminal regions appeared to be under purifying selection, but the N-terminal region presented seven sites under positive selection, with a dN/dS ratio 5-fold greater than that of the C-terminal region. Serological reactivity against monoclonal antibodies supports a higher occurrence probability for linear epitopes in the N-terminal region, as inferred by the sequence analysis. The obtained results reflect an unusual evolutionary behavior of the CP that, together with protein serological reactivity, suggests biological significance of the observed variability. Highlights: The occurrence of GLRaV-4 strains -5, -6 and -9 was recorded in Argentina. The coat protein sequence showed a highly variable N-terminal region among the strains. An evolutionary analysis inferred the occurrence of sites under positive diversifying selection pressure. The most variable region of the coat protein presented the highest probability of occurrence of linear epitopes.This research studied serological relationships and genetic diversity of Argentinean isolates of grapevine leafroll-associated virus-4 (GLRaV-4). Phylogenetic analysis of coat protein (CP) sequences from 19 local isolates revealed clustering with the previously described GLRaV-4 strain 5, strain 6, and strain 9 groups. Evolutionary sequence analysis of the obtained and database-available sequences showed evidence of recombination events. Additionally, both CP N- and C-terminal regions appeared to be under purifying selection, but the N-terminal region presented seven sites under positive selection, with a dN/dS ratio 5-fold greater than that of the C-terminal region. Serological reactivity against monoclonal antibodies supports a higher occurrence probability for linear epitopes in the N-terminal region, as inferred by the sequence analysis. The obtained results reflect an unusual evolutionary behavior of the CP that, together with protein serological reactivity, suggests biological significance of the observed variability. Highlights: The occurrence of GLRaV-4 strains -5, -6 and -9 was recorded in Argentina. The coat protein sequence showed a highly variable N-terminal region among the strains. An evolutionary analysis inferred the occurrence of sites under positive diversifying selection pressure. The most variable region of the coat protein presented the highest probability of occurrence of linear epitopes

Seguimiento clínico de los pacientes anticoagulados con ACODs en el sector III de Zaragoza. Datos de vida real

La aparición en los últimos años de los anticoagulantes orales de acción directa ha supuesto una revolución en el tratamiento de los pacientes con fibrilación auricular. Los estudios pivotales han demostrado igualdad frente a warfarina en términos de eficacia y superioridad en términos de seguridad en la prevención del ictus y la embolia sistémica en pacientes con fibrilación auricular no valvular (FANV). Desde la unidad de tratamiento anticoagulante del HCU Lozano Blesa de Zaragoza se lleva a cabo un registro con todos aquellos pacientes que están en tratamiento con los anticoagulantes de acción directa. El objetivo de este trabajo es describir los datos del registro recogidos hasta el momento y, asimismo, compararlos con los datos de los ensayos clínicos principales y datos de vida real publicados

Educación inclusiva. Creando culturas

En este trabajo se desarrolla el concepto de inclusión basándonos en dos pilares fundamentales: aceptación de la integración como algo positivo que se puede convertir en oportunidades de aprendizajes y la comunidad, entendida como un apoyo total de todos los miembros que forman parte del centro, desde alumnos a otras instituciones. En él se tratan aspectos teóricos en los que posteriormente me basaré para analizar, a través del Index (índice de inclusión) de Ainscow y Booth, la dimensión más social y cultural de un centro, que se caracteriza por su carácter inclusivo. Con este instrumento intentaré valorar el grado de inclusividad del mismo y cómo da respuesta a las necesidades que presentan los alumnos

Relevamiento de Thysanoptera en cultivos horticolas, como vectores potenciales de Tospovirus en Mendoza, Argentina

Tospoviruses severely affect tomato, lettuce and other crops in Argentina' and are thrips transmitted in nature. The epidemiology of tospoviruses is closely related with vector's ecology. The aim of this study was to know the Thysanoptera fauna in and around the tomato ecosystem of the Mendoza province" and to find rearingmethods for the more common species. Thrips were collected frorn 51 host plants and 19 species were identified, five of which [Bregmatothrips venustus Hood, Chirothrips frontalis Williams, C. texanus (Andre), Frankliniella frumenti Moulton, and F. valdiviana Sakimura &O'Neill] are new records for Mendoza and Karnyothrips longiceps (Hood) for Argentina. Three out of the eight species reported to transmit tospoviruses are present in Mendoza: F. schultzei (Trybom). F. occidentalis (Pergande) and Thrips tabaci Lindeman. Rearing rnethods were developed for eight species: Aptinothrips rufus (Haliday), F. australis Morganl F. frumenti, F. gemina (Bagnall), F. occidentelis, F. schultzei, Haplothrips trellesi Moulton, and T. tabaci. Colonies were reared from a single female in a growth room at 25° (± 2) and L 12: 0 12.En la Argentina el tomate, la lechuga y otras hortalizas, son severamente atacadas por virus del genero Tospovirus. Estos virus, son transmitidos por trips en condiciones naturales. La epidemiología de los tospovirus, esta estrechamente relacionada con la ecología de los vectores. El propósito de estetrabajo, fue conocer la fauna Thysanoptera en el ecosistema de tomate y su area circundante, en la provincia de Mendoza, y tambien poner a punto metodos de cría para las especies mas abundantes. Se coleccionaron trips en 51 huespedes y se identificaron 19 especies de tisanópteros, cinco de los cuales [Bregmatothrips venustus Hood, Chirothrips frontalis Williams, C. texanus (Andre), Frankliniella frumenti Moulton y F. valdiviana Sakimura & 0' Neill] son citas nuevas para Mendoza, y una Karnyothrips longiceps(Hood) para la Argentina. Ocho especies han sido registradas en la bibliografia mundial como vectoras de tospovirus, de las cuales sólo tres estan presentes en Mendoza: F. schultzei (Tryborn), F: occidentalis (Pergande) y Thrips tabaciLindeman. Seajustaron metodos de cría para ocho de las especies identificadas: Aptinothrips rufus(Haliday), F. australisMorgan, F.frumenti, F. gemina (Bagnall), F. occidentalis, F. schultzei, Haplothrips trellesi Moulton y T. tabaci. Las colonias se originaron en una sola hernbra, en carnara de crecimiento a 25°C (± 2) con fotoperíodo de 12 horas.Facultad de Ciencias Naturales y Muse

Thermal liquid biopsy (Tlb): A predictive score derived from serum thermograms as a clinical tool for screening lung cancer patients

Risk population screening programs are instrumental for advancing cancer management and reducing economic costs of therapeutic interventions and the burden of the disease, as well as increasing the survival rate and improving the quality of life for cancer patients. Lung cancer, with high incidence and mortality rates, is not excluded from this situation. The success of screening programs relies on many factors, with some of them being the appropriate definition of the risk population and the implementation of detection techniques with an optimal discrimination power and strong patient adherence. Liquid biopsy based on serum or plasma detection of circulating tumor cells or DNA/RNA is increasingly employed nowadays, but certain limitations constrain its wide application. In this work, we present a new implementation of thermal liquid biopsy (TLB) for lung cancer patients. TLB provides a prediction score based on the ability to detect plasma/serum proteome alterations through calorimetric thermograms that strongly correlates with the presence of lung cancer disease (91% accuracy rate, 90% sensitivity, 92% specificity, diagnostic odds ratio 104). TLB is a quick, minimally-invasive, low-risk technique that can be applied in clinical practice for evidencing lung cancer, and it can be used in screening and monitoring actions