Mouse Embryo Development in the Presence of Capsaicin

Capsaicin is the pungent agent found in hot peppers of the Capsicum genus. It is a potent neurotoxin that stimulates the degranulation and degeneration of C-afferent neurons. Capsaicin is widely used as a food condiment and medicine. Human exposure of capsaicin can exceed levels shown to be neurotoxic in laboratory animals. Additionally, capsaicin can cross the blood/placenta barrier and affect an embryo in utero. In order to assay the potential for toxicity to human embryos, mouse embryos were exposed to capsaicin and the effect of the capsaicin on embryo development was measured. Embryos were co-cultured in Krebs medium with 1% ethanol and from 1 to 3mM capsaicin. The higher levels of capsaicin significantly inhibited embryo development. Post implantation fetuses were treated in the dam with 0.3, 0.6, 1.5, and 3 μmoles capsaicin, and tested for developmental defeds. No significant differences were found between the capsaicin treated fetuses and the control fetuses. Female mice were exposed to 3mM capsaicin subcutaneously, by mouth, and topically. Levels of capsaicin in their blood serum were measured by high-pressure liquid chromatography. No significant levels of capsaicin were detected. It was concluded that any deleterious effect of capsaicin on embryo or fetal development depends on very high dosages and that these levels are unlikely to be encountered in the blood. Also, capsaicin had no deleterious effect on cartilage, bone, or limb development in mice

Reactivación participativa de espacios colectivos en la colonia Loma Linda, Puebla

"El presente texto constituye el resultado de la investigación titulada “Reactivación Participativa de Espacios Colectivos en la Colonia Loma Linda, Puebla”, realizada para obtener el grado de Maestro en Ordenamiento del Territorio, en el período 2017-2019. Consiste en una aproximación a través de una investigación-acción participativa a procesos de reactivación de espacios públicos urbanos en la ciudad de Puebla, México.

Use of three joints as predictors of carcass and body fat depots in Blanca Celtibérica goats

Nineteen adult goats of the Blanca Celtibérica breed - in non productive stage - from the experimental flock of Servicio de Investigación Agraria (Diputación General de Aragón) were scored using the lumbar, breast and tail palpation. Sternal triangle joint, lumbar square jointt a ailn tdha t were handled to assess body condition scores were dissected into muscle, bone subcutaneous fat and intermuscular fat. Regression analyses were developed for predicting carcass and body fat depots, using sternal triangle, lumbar square joint and tail compositions like independent variables. The best predictor of omental fat, subcutaneous fat, intermuscular fat, kidney fat, total body and total carcass fat depots was the totaol ff alut mbar square joint. On the other hand8 9, 80 and 86% (PS0,Ol) of the variationo f mesenteric, heart and udder fat depots, respectively, were accounted for by variation in intermuscular fat of sternal triangle joint. Finally, the subcutaneous fat weight of 1 tail vertebra accounted for 96% of the total variation of tail fat weight. In conclusion, the best predictor of the most important carcass and body fat depots (omental, subcutaneous, intermuscular, kidney, total body and total carcass fat) was the total fat of lumbar square. Utilisation de trois points de prélèvement comme prédicteurs des dépôts adipeux de la carcasse et du corps chez des chèvres de race Blanca Celtibérica". Un lot de 19 chèvres adultes de race Blanca Celtibérica, vides et taries, appartenant au troupeau expérimental du Service de la Recherche Agronomique (Gouvernement Autonome d'Aragon), ont été notées par la palpation au niveau lombaire, setet crnaauld al. Le triangle sternal, le carré lombaire et la queue ont été soumis à dissection en muscle, os, gras sous-cutané et intermusculaire. L 'analyse des régressionsé até utilisée pour prédire les dépôts adipeux de la carcaests edu corps de l'animal moyennant l'utilisation de la composition du triangle sterna/, du carré lombaire et de la queue comme variables indépendantes. Le meilleur prédicteur des graisses omentales, sous-cutanées, intermusculaires, rénales totales du corps, et totales de la carcasse é até le gras total du carré lombaire. D'autre part, 89, e8t0 8 6% (E0,Ol) de la variation des dépôts de gras mésentérique, du coeur et de la mamelle respectivement, ont eté expliqués par la variation du gras intermusculaire du triangle sternal. Comme conclusion, le meilleur prédicteur des dépôts adipeux de la carcasse et du corps les plus importants (omental, sous-cutané, intermusculaire, rénal, total du corps et total de la carcasse) a été le gras total du carré lombaire

Nivel de satisfacción laboral en las y los colaboradores del área del call center de la financiera EDPYME GMG - Servicios Perú S.A en el distrito de Surco

La presente investigación tiene por objetivo determinar el nivel de satisfacción laboral en las y los colaboradores del área de call center de la financiera EDPYME GMG Servicios Perú S.A en el distrito de Surco. El estudio corresponde a una investigación de tipo descriptiva, de tipo cuantitativo de acuerdo al manejo de los datos. El diseño de la investigación es no experimental, de corte transversal. El universo de la población se conforma por 66 colaboradores del área de call center que laboran a diario en la financiera EDPYME GMG - Servicios Perú S.A. La muestra está constituida por 62 colaboradores. La técnica que se empleó en dicha investigación para la recolección de información fue la encuesta y el instrumento utilizado fue un cuestionario de evaluación de satisfacción laboral. Los resultados obtenidos determinaron que las y los colaboradores presentan predominantemente en el desarrollo profesional un nivel nada satisfecho, en la dimensión clima organizacional demostraron un nivel medianamente satisfecho y en la dimensión condiciones laborales presentan un nivel nada satisfecho. Se establece como conclusión que la satisfacción laboral en las y los colaboradores del área del call center de la financiera EDPYME se encuentra en 52% en un nivel nada satisfecho, debido principalmente a las inadecuadas condiciones laborales y a las pocas oportunidades de desarrollo profesional.The objective of this research is to determine the level of job satisfaction in the employees of the call center area of the EDPYME GMG Servicios Perú SA financial district in the district of Surco. The study corresponds to a descriptive, quantitative type of research according to the handling of the data. The research design is non-experimental, crosssectional. The universe of the population consists of 66 employees of the call center area who work daily in the financial EDPYME GMG - Servicios Perú S.A. The sample consists of 62 employees. The technique used in this investigation for the collection of information was the survey and the instrument used was a questionnaire to assess job satisfaction. The obtained results determined that the collaborators have a predominantly professional level at a level not satisfied, in the organizational climate dimension they showed a moderately satisfied level and in the dimension working conditions present a level not satisfied at all. It is established as a conclusion that job satisfaction in the employees of the call center area of the EDPYME financial institution is at 52% at a level that is not at all satisfied, mainly due to the inadequate working conditions and the few professional development opportunities

Rapid assessment of the effect of ciprofloxacin on chromosomal DNA from Escherichia coli using an in situ DNA fragmentation assay

<p>Abstract</p> <p>Background</p> <p>Fluoroquinolones are extensively used antibiotics that induce DNA double-strand breaks (DSBs) by trapping DNA gyrase and topoisomerase IV on DNA. This effect is usually evaluated using biochemical or molecular procedures, but these are not effective at the single-cell level. We assessed ciprofloxacin (CIP)-induced chromosomal DNA breakage in single-cell <it>Escherichia coli </it>by direct visualization of the DNA fragments that diffused from the nucleoid obtained after bacterial lysis in an agarose microgel on a slide.</p> <p>Results</p> <p>Exposing the <it>E. coli </it>strain TG1 to CIP starting at a minimum inhibitory concentration (MIC) of 0.012 μg/ml and at increasing doses for 40 min increased the DNA fragmentation progressively. DNA damage started to be detectable at the MIC dose. At a dose of 1 μg/ml of CIP, DNA damage was visualized clearly immediately after processing, and the DNA fragmentation increased progressively with the antibiotic incubation time. The level of DNA damage was much higher when the bacteria were taken from liquid LB broth than from solid LB agar. CIP treatment produced a progressively slower rate of DNA damage in bacteria in the stationary phase than in the exponentially growing phase. Removing the antibiotic after the 40 min incubation resulted in progressive DSB repair activity with time. The magnitude of DNA repair was inversely related to CIP dose and was noticeable after incubation with CIP at 0.1 μg/ml but scarce after 10 μg/ml. The repair activity was not strictly related to viability. Four <it>E. coli </it>strains with identified mechanisms of reduced sensitivity to CIP were assessed using this procedure and produced DNA fragmentation levels that were inversely related to MIC dose, except those with very high MIC dose.</p> <p>Conclusion</p> <p>This procedure for determining DNA fragmentation is a simple and rapid test for studying and evaluating the effect of quinolones.</p

Atom abstraction in the interaction of F₂ and XeF₂ with Si(100)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1997.Includes bibliographical references.by David Gosalvez-Blanco.Ph.D

Dry biobanking as a conservation tool in the Anthropocene

Species are going extinct at an alarming rate, termed by some as the sixth mass extinction event in the history of Earth. Many are the causes for this but in the end, all converge to one entity – humans. Since we are the cause, we also hold the key to making the change. Any change, however, will take time, and for some species this could be too long. While working on possible solutions, we also have the responsibility to buy time for those species on the verge of extinction. Genome resource banks, in the form of cryobanks, where samples are maintained under liquid nitrogen, are already in existence but they come with a host of drawbacks. Biomimicry – innovation inspired by Nature, has been a huge source for ideas. Searching methods that Nature utilizes to preserve biological systems for extended periods of time, we realize that drying rather than freezing is the method of choice. We thus argue here in favor of preserving at least part of the samples from critically endangered species in dry biobanks, a much safer, cost-effective, biobanking approach

Level set implementation for the simulation of anisotropic etching: application to complex MEMS micromachining

The use of atomistic methods, such as the continuous cellular automaton (CCA), is currently regarded as an accurate and efficient approach for the simulation of anisotropic etching in the development of micro-electro-mechanical systems (MEMS). However, whenever the targeted etching condition is modified (e. g. by changing the substrate material, etchant type, concentration and/or temperature) this approach requires performing a time-consuming recalibration of the full set of internal atomistic rates defined within the method. Based on the level set (LS) approach as an alternative and using the experimental data directly as input, we present a fully operational simulator that exhibits similar accuracy to the latest CCA models. The proposed simulator is tested by describing a wide range of silicon and quartz MEMS structures obtained in different etchants through complex processes, including double-sided etching as well as different mask patterns during different etching steps and/or simultaneous masking materials on different regions of the substrate. The results demonstrate that the LS method is able to simulate anisotropic etching for complex MEMS processes with similar computational times and accuracy as the atomistic models.This work has been supported by the Spanish FPI-MICINN BES-2011-045940 grant and the Ramon y Cajal Fellowship Program by the Spanish Ministry of Science and Innovation. Also, we acknowledge support by the JAE-Doc grant from the Junta para la Ampliacion de Estudios program co-funded by FSE and the Professor Partnership Program by NVIDIA Corporation.Montoliu, C.; Ferrando Jódar, N.; Gosalvez Ayuso, MA.; Cerdá Boluda, J.; Colom Palero, RJ. (2013). 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A., Yan Xing, & Sato, K. (2008). Analytical Solution of the Continuous Cellular Automaton for Anisotropic Etching. Journal of Microelectromechanical Systems, 17(2), 410-431. doi:10.1109/jmems.2008.916339Ferrando, N., Gosálvez, M. A., Cerdá, J., Gadea, R., & Sato, K. (2011). Faster and exact implementation of the continuous cellular automaton for anisotropic etching simulations. Journal of Micromechanics and Microengineering, 21(2), 025021. doi:10.1088/0960-1317/21/2/025021Ferrando, N., Gosálvez, M. A., Cerdá, J., Gadea, R., & Sato, K. (2011). Octree-based, GPU implementation of a continuous cellular automaton for the simulation of complex, evolving surfaces. Computer Physics Communications, 182(3), 628-640. doi:10.1016/j.cpc.2010.11.004Moktadir, Z., & Camon, H. (1997). Monte Carlo simulation of anisotropic etching of silicon: investigation of surface properties. 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Low coverage surface diffusion in complex, periodic energy landscapes. Part II: Analytical solution for systems with asymmetric hops

This is part II in a series of two papers that introduce a general expression for the tracer diffusivity in complex, periodic energy landscapes with M distinct hop rates in one-, two-, and three-dimensional diluted systems (low coverage, single-tracer limit). While Part I [Gosálvez et al., Phys. Rev. B 93, 075429 (2016)] focuses on the analysis of diffusion in systems where the end sites of the hops are located symmetrically with respect to the hop origins (symmetric hops), as encountered in many ideal surfaces and bulk materials, this report (Part II) presents a more general approach to determining the tracer diffusivity in systems where the end sites can be located asymmetrically with respect to the hop origins (asymmetric hops), as observed in reconstructed and/or chemically modified surfaces and/or bulk materials. The obtained diffusivity formulas for numerous systems are validated against kinetic Monte Carlo simulations and previously reported analytical expressions based on the continuous-time random walk (CTRW) method. The proposed method corrects some of the CTRW formulas and provides new expressions for difficult cases that have not been solved earlier. This demonstrates the ability of the proposed formalism to describe tracer diffusion

Por la Ciudad de Alicante y Gines Gosaluez escriuano... y iurados de dicha Ciudad con el dotor Victoriano Tredos y Pasqual

Sign.: A-F2, G3Port. grab. xil. representando a San Francisco de Paul