PITX2 gain-of-function induced defects in mouse forelimb development

BACKGROUND: Limb development and patterning originate from a complex interplay between the skeletal elements, tendons, and muscles of the limb. One of the genes involved in patterning of limb muscles is the homeobox transcription factor Pitx2 but its role in forelimb development is uncharacterized. Pitx2 is expressed in the majority of premature presumptive forelimb musculature at embryonic day 12.5 and then maintained throughout embryogenesis to adult skeletal muscle. RESULTS: To further study the role of Pitx2 in forelimb development we have generated transgenic mice that exhibit a pulse of PITX2 over-expression at embryonic day 13.5 and 14.5 in the developing forelimb mesenchyme. These mice exhibit a distal misplacement of the biceps brachii insertion during embryogenesis, which twists the forelimb musculature resulting in severe skeletal malformations. The skeletal malformations have some similarities to the forearm deformities present in Leri-Weill dyschondrosteosis. CONCLUSION: Taken together, the tendon, muscle, and bone anomalies further support a role of Pitx2 in forelimb development and may also shed light on the interaction between the skeletal elements and muscles of the limb during embryogenesis

Measurement of the Transverse Polarization of Electrons Emitted in Free Neutron Decay

Both components of the transverse polarization of electrons emitted in the beta-decay of polarized, free neutrons have been measured. The T-odd, P-odd correlation coefficient quantifying the component perpendicular to the decay plane defined by neutron polarization and electron momentum, was found to be R=0.008 +/- 0.015 +/-0.005. This value is consistent with time reversal invariance, and significantly improves limits on the relative strength of imaginary scalar couplings in the weak interaction. The value obtained for the correlation coefficient associated with the electron polarization component contained within the decay plane N=0.056 +/- 0.011 +/- 0.005, agrees with the Standard Model expectation, providing an important sensitivity test of the experimental setup.Comment: 4 pages, 4 figure

Measurement of Transverse Polarization of Electrons Emitted in Free Neutron Decay

The final analysis of the experiment determining both components of the transverse polarization of electrons ($\sigma_{T_{1}}$, $\sigma_{T_{2}}$) emitted in the $\beta$-decay of polarized, free neutrons is presented. The T-odd, P-odd correlation coefficient quantifying $\sigma_{T_{2}}$, perpendicular to the neutron polarization and electron momentum, was found to be $R=$ 0.004$\pm0.012\pm$0.005. This value is consistent with time reversal invariance, and significantly improves both earlier result and limits on the relative strength of imaginary scalar couplings in the weak interaction. The value obtained for the correlation coefficient associated with $\sigma_{T_{1}}$, $N=$ 0.067$\pm0.011\pm$0.004, agrees with the Standard Model expectation, providing an important sensitivity test of the experimental setup. The present result sets constraints on the imaginary part of scalar and tensor couplings in weak interaction. Implications for parameters of the leptoquark exchange model and minimal supersymmetric model (MSSM) with R-parity violation are discussed

Early vertebrate chromosome duplications and the evolution of the neuropeptide Y receptor gene regions

<p>Abstract</p> <p>Background</p> <p>One of the many gene families that expanded in early vertebrate evolution is the neuropeptide (NPY) receptor family of G-protein coupled receptors. Earlier work by our lab suggested that several of the NPY receptor genes found in extant vertebrates resulted from two genome duplications before the origin of jawed vertebrates (gnathostomes) and one additional genome duplication in the actinopterygian lineage, based on their location on chromosomes sharing several gene families. In this study we have investigated, in five vertebrate genomes, 45 gene families with members close to the NPY receptor genes in the compact genomes of the teleost fishes <it>Tetraodon nigroviridis </it>and <it>Takifugu rubripes</it>. These correspond to <it>Homo sapiens </it>chromosomes 4, 5, 8 and 10.</p> <p>Results</p> <p>Chromosome regions with conserved synteny were identified and confirmed by phylogenetic analyses in <it>H. sapiens, M. musculus, D. rerio, T. rubripes </it>and <it>T. nigroviridis</it>. 26 gene families, including the NPY receptor genes, (plus 3 described recently by other labs) showed a tree topology consistent with duplications in early vertebrate evolution and in the actinopterygian lineage, thereby supporting expansion through block duplications. Eight gene families had complications that precluded analysis (such as short sequence length or variable number of repeated domains) and another eight families did not support block duplications (because the paralogs in these families seem to have originated in another time window than the proposed genome duplication events). RT-PCR carried out with several tissues in <it>T. rubripes </it>revealed that all five NPY receptors were expressed in the brain and subtypes Y2, Y4 and Y8 were also expressed in peripheral organs.</p> <p>Conclusion</p> <p>We conclude that the phylogenetic analyses and chromosomal locations of these gene families support duplications of large blocks of genes or even entire chromosomes. Thus, these results are consistent with two early vertebrate tetraploidizations forming a paralogon comprising human chromosomes 4, 5, 8 and 10 and one teleost tetraploidization. The combination of positional and phylogenetic data further strengthens the identification of orthologs and paralogs in the NPY receptor family.</p

Free neutron decay and time reversal violation

Both components of the transverse electron polarization have been measured in free neutron decay. The T-odd, P-odd correlation coefficient associated with polarization component perpendicular to the neutron polarization and electron momentum, was found to be R = 0:006 0:012 0:005. This value is consistent with time reversal invariance, and significantly improves limits on the relative strength of imaginary scalar couplings in the weak interaction. The value obtained for the T-even, P-even correlation coefficient connected with the second transversal polarization component, N = 0:065 0:012 0:004, agrees with the Standard Model expectation providing an important sensitivity test of the experimental setup

The modern pollen-vegetation relationship of a tropical forest-savannah mosaic landscape, Ghana, West Africa

Transitions between forest and savannah vegetation types in fossil pollen records are often poorly understood due to over-production by taxa such as Poaceae and a lack of modern pollen-vegetation studies. Here, modern pollen assemblages from within a forest-savannah transition in West Africa are presented and compared, their characteristic taxa discussed, and implications for the fossil record considered. Fifteen artificial pollen traps were deployed for 1 year, to collect pollen rain from three vegetation plots within the forest-savannah transition in Ghana. High percentages of Poaceae and Melastomataceae/Combretaceae were recorded in all three plots. Erythrophleum suaveolens characterised the forest plot, Manilkara obovata the transition plot and Terminalia the savannah plot. The results indicate that Poaceae pollen influx rates provide the best representation of the forest-savannah gradient, and that a Poaceae abundance of >40% should be considered as indicative of savannah-type vegetation in the fossil record

Improving Photoelectron Counting and Particle Identification in Scintillation Detectors with Bayesian Techniques

Many current and future dark matter and neutrino detectors are designed to measure scintillation light with a large array of photomultiplier tubes (PMTs). The energy resolution and particle identification capabilities of these detectors depend in part on the ability to accurately identify individual photoelectrons in PMT waveforms despite large variability in pulse amplitudes and pulse pileup. We describe a Bayesian technique that can identify the times of individual photoelectrons in a sampled PMT waveform without deconvolution, even when pileup is present. To demonstrate the technique, we apply it to the general problem of particle identification in single-phase liquid argon dark matter detectors. Using the output of the Bayesian photoelectron counting algorithm described in this paper, we construct several test statistics for rejection of backgrounds for dark matter searches in argon. Compared to simpler methods based on either observed charge or peak finding, the photoelectron counting technique improves both energy resolution and particle identification of low energy events in calibration data from the DEAP-1 detector and simulation of the larger MiniCLEAN dark matter detector.Comment: 16 pages, 16 figure

Radon backgrounds in the DEAP-1 liquid-argon-based Dark Matter detector

The DEAP-1 \SI{7}{kg} single phase liquid argon scintillation detector was operated underground at SNOLAB in order to test the techniques and measure the backgrounds inherent to single phase detection, in support of the \mbox{DEAP-3600} Dark Matter detector. Backgrounds in DEAP are controlled through material selection, construction techniques, pulse shape discrimination and event reconstruction. This report details the analysis of background events observed in three iterations of the DEAP-1 detector, and the measures taken to reduce them. The $^{222}$Rn decay rate in the liquid argon was measured to be between 16 and \SI{26}{\micro\becquerel\per\kilogram}. We found that the background spectrum near the region of interest for Dark Matter detection in the DEAP-1 detector can be described considering events from three sources: radon daughters decaying on the surface of the active volume, the expected rate of electromagnetic events misidentified as nuclear recoils due to inefficiencies in the pulse shape discrimination, and leakage of events from outside the fiducial volume due to imperfect position reconstruction. These backgrounds statistically account for all observed events, and they will be strongly reduced in the DEAP-3600 detector due to its higher light yield and simpler geometry