Prevalence of dens invaginatus assessed by CBCT : systematic review and meta-analysis

Dens invaginatus is a developmental dental anomaly resulting from an invagination of dental tissues folding from the outer surface towards dental pulp. The aim of this systematic review and meta-analysis was to determine the prevalence of dens invaginatu

Whole-Genome Sequences of Five Acinetobacter baumannii Strains From a Child With Leukemia M2

Acinetobacter baumannii is an opportunistic pathogen and is one of the primary etiological agents of healthcare-associated infections (HAIs). A. baumannii infections are difficult to treat due to the intrinsic and acquired antibiotic resistance of strains of this bacterium, which frequently limits therapeutic options. In this study, five A. baumannii strains (810CP, 433H, 434H, 483H, and A-2), all of which were isolated from a child with leukemia M2, were characterized through antibiotic susceptibility profiling, the detection of genes encoding carbapenem hydrolyzing oxacillinases, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), adherence and invasion assays toward the A549 cell line, and the whole-genome sequence (WGS). The five strains showed Multidrug resistant (MDR) profiles and amplification of the blaOXA-23 gene, belonging to ST758 and grouped into two PFGE clusters. WGS of 810CP revealed the presence of a circular chromosome and two small plasmids, pAba810CPa and pAba810CPb. Both plasmids carried genes encoding the Sp1TA system, although resistance genes were not identified. A gene-by-gene comparison analysis was performed among the A. baumannii strains isolated in this study and others A. baumannii ST758 strains (HIMFG and INCan), showing that 86% of genes were present in all analyzed strains. Interestingly, the 433H, 434H, and 483H strains varied by 8–10 single-nucleotide variants (SNVs), while the A2 and 810CP strains varied by 46 SNVs. Subsequently, an analysis using BacWGSTdb showed that all of our strains had the same resistance genes and were ST758. However, some variations were observed in relation to virulence genes, mainly in the 810CP strain. The genes involved in the synthesis of hepta-acylated lipooligosaccharides, the pgaABCD locus encoding poly-β-1-6-N-acetylglucosamine, the ompA gene, Csu pili, bap, the two-component system bfms/bfmR, a member of the phospholipase D family, and two iron-uptake systems were identified in our A. baumannii strains genome. The five A. baumannii strains isolated from the child were genetically different and showed important characteristics that promote survival in a hospital environment. The elucidation of their genomic sequences provides important information for understanding their epidemiology, antibiotic resistance, and putative virulence factors

Soy Niña

Este libro pretende contribuir al reencuentro de la educación con esas finalidades que verdaderamente importan a una niña o un niño: ser feliz, jugar, vivir juntos y (no) aprender. Para ello hemos puesto el arte, nuestras experiencias y el saber acumulado al servicio del disfrute, el cuestionamiento, el análisis crítico y la construcción común de un presente deseable. Un texto colaborativo coordinado por Ignacio Calderón Almendros y realizado por alumnado de Educación y Cambio Social en el Grado en Educación Infantil de la Universidad de Málaga

. 34. Arqueología

Este número de Arqueología incorpora una amplia gama de temas de la historia mesoamericana y novohispana, pero además incluye algunos trabajos de corte teórico-metodológico, bastante escasos hasta ahora en la revista, como lo comprueba una rápida revisión cuantitativa en el índice general de recién publicación (Arqueología 31:152-154). Esperemos que este hecho represente el comienzo de un cambio en este sentido y que en el futuro haya más esfuerzos de reflexión sobre las propuestas teóricas a partir de las cuales construimos nuestros datos. El primer trabajo, de Benavides y Novelo, nos presenta una caracterización arquitectónica, espacial y temporal del asentamientos de Balché, en el noreste de Campeche, con propuestas para su conservación. El siguiente es una colaboración colectiva de Roberto Lunagómez, Xóchitl León y Nelly Núñez, que plantea la reconstrucción y discusión de la secuencia ocupacional y cerámica del sitio Clásico terminal de Medias Aguas, en el sur de Veracruz, así como de su papel a escala regional. El artículo de Pérez Negrete, a partir del estudio de las evidencias del Clásico y de la transición al Epiclásico en el Cerro de La Estrella en la cuenca de México, propone la conformación de centros regionales con el fortalecimiento de elites locales, lo que favorece la aparición de modelos culturales alternos al teotihuacano. Siguiendo con el tema del Epiclásico en la Altiplanicie Central, Mónica Zamora intenta un acercamiento a la estructura urbanística de Cantona y a sus cambios en el tiempo a partir de sus 25 juegos de pelota, de los que analiza la forma, la distribución y los arreglos arquitectónicos. En el trabajo de Guevara y Rojas, el análisis y distribución de complejos cerámicos permite construir un modelo de interacción entre cacicazgos de la Costa Grande de Guerrero, donde la competencia entre elites origina un proceso de regionalización. Pijoan y colaboradores, mediante la determinación de las sustancias utilizadas para ennegrecer las piezas dentarias de un entierro en Tlatelolco, en la Ciudad de México, establecen connotaciones culturales e históricas muy interesantes, donde se puede confrontar la narrativa histórica con la observación de los contextos arqueológicos. Carlos Salas también utiliza la documentación histórica y moderna, y la información arqueológica para describir, por medio de planos, la evolución arquitectónica y de uso del suelo del espacio que ocupó el convento de La Encarnación, en la Ciudad de México. Por último, el trabajo de Jesús Sánchez nos hace reflexionar sobre el uso de conceptos que, tomados de otras disciplinas, como la historia del arte, se han vuelto elementos clave de los discursos arqueológicos. El autor propone sustituir el concepto de estilo por el de carácter con el fin de clarificar el manejo de la categoría de tipo arqueológico. Finalmente, los invitamos a seguir colaborando con esta revista, su revista, cumpliendo puntualmente con los requisitos de publicación enunciados en la invitación a los colaboradores, ya que esto nos permitirá agilizar el proceso de publicación.</p

Compilación de Proyectos de Investigacion de 1984-2002

Instituto Politecnico Nacional. UPIICS

A 12-gene pharmacogenetic panel to prevent adverse drug reactions: an open-label, multicentre, controlled, cluster-randomised crossover implementation study

© 2023Background: The benefit of pharmacogenetic testing before starting drug therapy has been well documented for several single gene–drug combinations. However, the clinical utility of a pre-emptive genotyping strategy using a pharmacogenetic panel has not been rigorously assessed. Methods: We conducted an open-label, multicentre, controlled, cluster-randomised, crossover implementation study of a 12-gene pharmacogenetic panel in 18 hospitals, nine community health centres, and 28 community pharmacies in seven European countries (Austria, Greece, Italy, the Netherlands, Slovenia, Spain, and the UK). Patients aged 18 years or older receiving a first prescription for a drug clinically recommended in the guidelines of the Dutch Pharmacogenetics Working Group (ie, the index drug) as part of routine care were eligible for inclusion. Exclusion criteria included previous genetic testing for a gene relevant to the index drug, a planned duration of treatment of less than 7 consecutive days, and severe renal or liver insufficiency. All patients gave written informed consent before taking part in the study. Participants were genotyped for 50 germline variants in 12 genes, and those with an actionable variant (ie, a drug–gene interaction test result for which the Dutch Pharmacogenetics Working Group [DPWG] recommended a change to standard-of-care drug treatment) were treated according to DPWG recommendations. Patients in the control group received standard treatment. To prepare clinicians for pre-emptive pharmacogenetic testing, local teams were educated during a site-initiation visit and online educational material was made available. The primary outcome was the occurrence of clinically relevant adverse drug reactions within the 12-week follow-up period. Analyses were irrespective of patient adherence to the DPWG guidelines. The primary analysis was done using a gatekeeping analysis, in which outcomes in people with an actionable drug–gene interaction in the study group versus the control group were compared, and only if the difference was statistically significant was an analysis done that included all of the patients in the study. Outcomes were compared between the study and control groups, both for patients with an actionable drug–gene interaction test result (ie, a result for which the DPWG recommended a change to standard-of-care drug treatment) and for all patients who received at least one dose of index drug. The safety analysis included all participants who received at least one dose of a study drug. This study is registered with ClinicalTrials.gov, NCT03093818 and is closed to new participants. Findings: Between March 7, 2017, and June 30, 2020, 41 696 patients were assessed for eligibility and 6944 (51·4 % female, 48·6% male; 97·7% self-reported European, Mediterranean, or Middle Eastern ethnicity) were enrolled and assigned to receive genotype-guided drug treatment (n=3342) or standard care (n=3602). 99 patients (52 [1·6%] of the study group and 47 [1·3%] of the control group) withdrew consent after group assignment. 652 participants (367 [11·0%] in the study group and 285 [7·9%] in the control group) were lost to follow-up. In patients with an actionable test result for the index drug (n=1558), a clinically relevant adverse drug reaction occurred in 152 (21·0%) of 725 patients in the study group and 231 (27·7%) of 833 patients in the control group (odds ratio [OR] 0·70 [95% CI 0·54–0·91]; p=0·0075), whereas for all patients, the incidence was 628 (21·5%) of 2923 patients in the study group and 934 (28·6%) of 3270 patients in the control group (OR 0·70 [95% CI 0·61–0·79]; p <0·0001). Interpretation: Genotype-guided treatment using a 12-gene pharmacogenetic panel significantly reduced the incidence of clinically relevant adverse drug reactions and was feasible across diverse European health-care system organisations and settings. Large-scale implementation could help to make drug therapy increasingly safe. Funding: European Union Horizon 2020