Experiencia religiosa de cómo un médico-científico experimenta a Dios

Este estudio se propone describir y profundizar cómo vive la experiencia religiosa un médicocientífico. Para ello, seguí un diseño metodológico que parte de un enfoque de investigación cualitativo, un diseño narrativo que articulado al método teológico ver-juzgar-actuar interpreta las experiencias humanas y religiosas de este médico-científico mediante el relato autobiográfico de la experiencia religiosa. Describo en él, momentos existenciales en contextos particulares y eventos significativos que adquieren sentido al reconocer la auto revelación de Dios en la cotidianidad de la vida personal y profesional, tomando conciencia de la falta de fundamento de un conflicto entre ciencia y fe.This study proposes to describe and to go deeper into how does a physician-scientist live the religious experience. To do so, we follow a methodological design that starts from a qualitative research focus; a narrative design that articulated to the seeing-judging-acting theological method interprets this physician-scientist´s human and religious experience through the autobiographical essay of the religious experience. We describe in it existential moments in particular contexts and significant events that make sense when recognizing God’s selfrevelation in the everydayness of the personal and professional life, becoming conscious of the lack of foundation of a conflict between science and faith.Licenciado (a) en Ciencias ReligiosasPregrad

A DNA hypermethylation profile reveals new potential biomarkers for the evaluation of prognosis in urothelial bladder cancer

DNA hypermethylation has emerged as a molecular biomarker for the evaluation of cancer diagnosis and prognosis. We define a methylation signature of bladder cancer and evaluate whether this profile assesses prognosis of patients. Genome-wide methylation analysis was performed on 70 tumor and 10 normal bladder samples. Hypermethylation status of 1505 CpGs present in the promoter region of 807 genes was studied. Thirty-three genes were significantly hypermethylated in >= 10% of the tumors. Three clusters of patients were characterized by their DNA methylation profile, one at higher risk of dead of disease (p = 0.0012). Association between cluster distribution and stage (p = 0.02) or grade (p = 0.02) was demonstrated. Hypermethylation of JAK3 and absence of hypermethylation of EYA4, GAT6, and SOX1 were associated with low-grade non-invasive disease. On the other hand, in high-grade invasive disease hypermethylation of CSPG2, HOXA11, HOXA9, HS3ST2, SOX1, and TWIST1 was associated with muscle invasiveness. A panel of hypermethylated genes including APC, CSPG2, EPHA5, EYA4, HOXA9, IPF1, ISL1, JAK3, PITX2, SOX1, and TWIST1 predicted cancer-specific survival and SOX1 (HR = 3.46), PITX2 (HR = 4.17), CSPG2 (HR = 5.35), and JAK3 hypermethylation (HR = 0.19) did so independently. Silencing of genes by hypermethylation is a common event in bladder cancer and could be used to develop diagnostic and prognostic markers. Combined hypermethylation of SOX1, PITX2, or CSPG2 signals patients at higher risk of death from bladder cancer

Familias homoparentales, más visibles y mejor aceptadas: efectos del matrimonio en España

En este artículo se pretende conocer el efecto en las familias homoparentales españolas de la aprobación del matrimonio entre personas del mismo sexo en 2005. Se entrevistó a 66 familias, encabezadas por parejas de lesbianas (48) o de gays (18), que llevaban juntas al menos desde 2004 y tenían hijos o hijas menores de edad en común. Se buscaba evaluar su grado de apertura (coming out) y la aceptación que percibían en distintos contextos antes y después de la legalización del matrimonio, con el objetivo de saber si se vieron influidos por ello. Los resultados obtenidos indican que, tras aprobarse la ley, aumentó tanto el grado de apertura como la aceptación percibida en todos los contextos estudiados (amistades, familia y ámbito laboral). Los datos se relacionan con la literatura científica y se discuten a la luz de sus implicaciones para el desarrollo de políticas de integración de las familias homoparentales.This article intends to know the effect on Spanish homoparental families of the approval of same sex marriage in 2005. We interviewed 66 same-sex families either made of gay fathers (18) or lesbian mothers (48) that were together at least since 2004 and had children under 18. The aim was to evaluate each context in relation with the degree of coming out before and after the marriage approval with the purpose of understanding whether there were any differences. The obtained results indicate that both coming out and acceptance increased after the law was approved in those three areas in which it was analyzed: among their friendships, at workplace and with their families. The data are related to scientific literature and are discussed in the light of their implications for the development of homoparental family integration policies

Epigenetic Regulation of Gfi1 in Endocrine-Related Cancers: A Role Regulating Tumor Growth

Prostate and breast cancer constitute the most common cancers among men and women worldwide. The aging population is one of the main risk factors for prostate and breast cancer development and accumulating studies link aging with epigenetic changes. Growth factor independence-1 (Gfi1) is a transcriptional repressor with an important role in human malignancies, including leukemia, colorectal carcinoma, and lung cancer, but its role in prostate and breast cancer is unknown. We have found that Gfi1 epigenetic silencing is a common event in prostate and breast cancer. Gfi1 re-expression in prostate and breast cancer cell lines displaying Gfi1 epigenetic silencing decreases cell proliferation, reduced colony formation density, and tumor growth in nude mice xenografts. In addition, we found that Gfi1 repress alpha 1-anti-trypsin (AAT) and alpha 1-anti-chymotrypsin (ACT) expression, two genes with important functions in cancer development, suggesting that Gfi1 silencing promotes tumor growth by increasing AAT and ACT expression in our system. Finally, Gfi1 epigenetic silencing could be a promising biomarker for prostate cancer progression because it is associated with shorter disease-free survival. In conclusion, our findings strongly indicate that Gfi1 epigenetic silencing in prostate and breast cancer could be a crucial step in the development of these two-well characterized endocrine related tumors.Instituto de Salud Carlos II

Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata

This article is part of the Research Topic Recent Advances on Grapevine-Microbe Interactions: From Signal Perception to Resistance Response[EN] Many phytopathogenic fungi produce necrosis and ethylene inducing peptide 1 (Nep1- like proteins or NLP) that trigger leaf necrosis and the activation of defense mechanisms. These proteins have been widely studied in plant pathogens as Moniliophthora perniciosa or Botrytis cinerea between others, but little is known about their biological roles in grapevine trunk pathogens. Advances in the sequencing of genomes of several fungi involved in grapevine trunk diseases have revealed that these proteins are present in several copies in their genomes. The aim of this project was to analyze the presence of genes encoding NLP proteins in the Diplodia seriata genome and to characterize their putative role as virulence factors associated to grapevine trunk diseases. In this study, we characterized four NLPs from Diplodia seriata. All proteins showed highly similar amino acid sequences and contained the characteristic peptide motifs of NLPs. DserNEPs slightly reduced the viability of Vitis vinifera L. cell cultures. The cytolytic activity from DserNEP1 was stronger than that from DserNEP2, even at low concentrations. Purified DserNEPs also produced necrosis in leaves when they were inoculated into micropropagules of V. vinifera L. This is the first record of Nep1-like proteins from a fungus associated with grapevine trunk diseases and also from a member of the Botryosphaeriaceae familySIThis work was supported by Bodegas Vega Sicilia S.A. (Valbuena de Duero, Valladolid, Spain

Proteomic and transcriptomic profiling reveals a link between the PI3K pathway and lower estrogen-receptor (ER) levels and activity in ER+ breast cancer

IntroductionAccumulating evidence suggests that both levels and activity of the estrogen receptor (ER) and the progesterone receptor (PR) are dramatically influenced by growth-factor receptor (GFR) signaling pathways, and that this crosstalk is a major determinant of both breast cancer progression and response to therapy. The phosphatidylinositol 3-kinase (PI3K) pathway, a key mediator of GFR signaling, is one of the most altered pathways in breast cancer. We thus examined whether deregulated PI3K signaling in luminal ER+ breast tumors is associated with ER level and activity and intrinsic molecular subtype.MethodsWe defined two independent molecular signatures of the PI3K pathway: a proteomic (reverse-phase proteomic array) PI3K signature, based on protein measurement for PI3K signaling intermediates, and a PI3K transcriptional (mRNA) signature based on the set of genes either induced or repressed by PI3K inhibitors. By using these signatures, we scored each ER+ breast tumor represented in multiple independent expression-profiling datasets (four mRNA, n = 915; one protein, n = 429) for activation of the PI3K pathway. Effects of PI3K inhibitor BEZ-235 on ER expression and activity levels and cell growth were tested by quantitative real-time PCR and cell proliferation assays.ResultsWithin ER+ tumors, ER levels were negatively correlated with the PI3K activation scores, both at the proteomic and transcriptional levels, in all datasets examined. PI3K signature scores were also higher in ER+ tumors and cell lines of the more aggressive luminal B molecular subtype versus those of the less aggressive luminal A subtype. Notably, BEZ-235 treatment in four different ER+ cell lines increased expression of ER and ER target genes including PR, and treatment with IGF-I (which signals via PI3K) decreased expression of ER and target genes, thus further establishing an inverse functional relation between ER and PI3K. BEZ-235 had an additional effect on tamoxifen in inhibiting the growth of a number of ER+ cell lines.ConclusionsOur data suggest that luminal B tumors have hyperactive GFR/PI3K signaling associated with lower ER levels, which has been correlated with resistance to endocrine therapy. Targeting PI3K in these tumors might reverse loss of ER expression and signaling and restore hormonal sensitivity

Diseñando el futuro de la docencia de grado en la facultad de odontología de la UCM

Fac. de OdontologíaFALSEsubmitte

Estado del Arte de la Investigación en Educación, Pedagogía, Didáctica, Aportes teóricos a las discusiones educativas sobre la diversidad, y Diversidad en procesos de aprendizaje y transformación de la escuela en la MEVI – 2018 - 2021.

Elaborar un estado del arte de la investigación en la MEVI en relación con la educación, la pedagogía, la didáctica, los aportes teóricos a las discusiones educativas sobre la diversidad y la diversidad en procesos de aprendizaje y transformación de la escuela entre 2018 y 2021El presente estado del arte evidencia los intereses investigativos y las conceptualizaciones derivadas en el campo de la educación la pedagogía, la didáctica, los aportes teóricos a las discusiones educativas sobre la diversidad y la diversidad en procesos de aprendizaje y transformación de la escuela, en las investigaciones realizadas en el programa de Maestría en Educación Virtual (en adelante MEVI), de la Corporación Universitaria Minuto de Dios ( en adelante UNIMINUTO), entre los años 2018 y 2021. Este proyecto sombrilla, se desarrolla bajo la sub-línea de Procesos educativos para la diversidad y la transformación cultural se realiza bajo un enfoque cualitativo descriptivo a partir de una selección aleatoria de 160 tesis de la MEVI. En el capítulo 1 se presenta una contextualización sobre el proceso investigativo dentro de la MEVI. También, se presentan los estudios nacionales e internacionales relacionados con la investigación; al tiempo que se presenta en detalle el planteamiento de objetivos con la intención de lograr categorías de estudio

Educación ambiental para el mantenimiento de cuerpos de agua contaminados mediante la IEP

La educación ambiental es un elemento utilizado desde las escuelas para crear consciencia en la comunidad educativa sobre las problemáticas de contaminación observadas en el contexto social donde habitan, el estudio tuvo como objetivo implementar la educación ambiental para el mantenimiento de cuerpos de agua contaminados mediante la investigación como estrategia pedagógica (IEP). La metodología se orientó bajo el enfoque cualitativo, utilizando el tipo de investigación acción, desde un diseño descriptivo, exploratorio. Para la recolección de datos se utilizaron técnicas como; observación participante y diario de campo. La población estuvo conformada por cincuenta (50) sujetos, para esto se realizó un muestreo no probabilístico de tipo intencional. Los resultados mostraron altos niveles de contaminación en el canal Tolima, por desecho de residuos sólidos, basuras, vertimiento de aguas residuales y uso de jabón y detergentes para lavar la ropa en el Rio, donde los estudiantes con la asesoría y acompañamiento de los maestros diseñaron estrategias educativas basadas en investigación para el mantenimiento de los cuerpos de agua contaminados del canal Tolima en Aracataca, apuntado a la formación de estudiantes con conciencia ciudadana, que aporten a la preservación del medioEnvironmental education is an element used by schools to create awareness in the educational community about the pollution problems observed in the social context where they live, the study aimed to implement environmental education for the maintenance of contaminated water bodies through research as a pedagogical strategy (IEP). The methodology was oriented under the qualitative approach, using the type of action research, from a descriptive, exploratory design. For the collection of data, techniques such as; participant observation and daily of field. The population consisted of fifty (50) subjects, for this a non-probabilistic sampling of intentional type was carried out. The results showed high levels of contamination in the Tolima channel, by waste of solid waste, garbage, dumping of wastewater and use of soap and detergents to wash clothes in the Rio, where the students with the advice and accompaniment of the teachers designed educational strategies based on research for the maintenance of contaminated water bodies of the Tolima channel in Aracataca, aimed at training students with citizen awareness, which contribute to the preservation of the environmen