Determinación de Cis-/Trans-Estilbenos en cera de panal de abeja

Las abejas juegan un papel fundamental no solo en lo relacionado con la producción de la colmena sino también en la polinización de plantas. Por todo ello, lo que las afecte negativamente, tendrá sin duda una gran repercusión en la Agricultura y el Medio Ambiente. La investigación sobre el tema se centra actualmente en la búsqueda de compuestos naturales o sintéticos alternativos al uso de antibióticos prohibidos actualmente para el tratamiento de infecciones. Un ejemplo claro es el uso de fitoalexinas, más en concreto de resveratrol que ha estado presente desde hace siglos en la medicina tradicional.Química AnalíticaMáster en Técnicas Avanzadas en Químic

Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit

Biochemistry; Molecular medicineBioquímica; Medicina molecularBioquímica; Medicina molecularImbalanced mitochondrial dNTP pools are known players in the pathogenesis of multiple human diseases. Here we show that, even under physiological conditions, dGTP is largely overrepresented among other dNTPs in mitochondria of mouse tissues and human cultured cells. In addition, a vast majority of mitochondrial dGTP is tightly bound to NDUFA10, an accessory subunit of complex I of the mitochondrial respiratory chain. NDUFA10 shares a deoxyribonucleoside kinase (dNK) domain with deoxyribonucleoside kinases in the nucleotide salvage pathway, though no specific function beyond stabilizing the complex I holoenzyme has been described for this subunit. We mutated the dNK domain of NDUFA10 in human HEK-293T cells while preserving complex I assembly and activity. The NDUFA10E160A/R161A shows reduced dGTP binding capacity in vitro and leads to a 50% reduction in mitochondrial dGTP content, proving that most dGTP is directly bound to the dNK domain of NDUFA10. This interaction may represent a hitherto unknown mechanism regulating mitochondrial dNTP availability and linking oxidative metabolism to DNA maintenance.We thank Dr, Luke Formosa (Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia) for his valuable advice and assistance on NDUFA10 molecular studies and Dr. Francesc Canals and his team (Proteomics Laboratory, Vall d’Hebron Institute of Oncology [VHIO], Universitat Autònoma de Barcelona, Barcelona, Spain) for their assistance with LC-MS/MS analyses. This work was supported by the Spanish Ministry of Industry, Economy and Competitiveness [grants BFU2014-52618-R, SAF2017-87506, and PID2020-112929RB-I00 to Y.C.], by the Spanish Instituto de Salud Carlos III [grants PI21/00554 and PMP15/00025 to R.M.], co-financed by the European Regional Development Fund (ERDF), and by an NHMRC Project grant to M.R. (GNT1164459)

Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit

Imbalanced mitochondrial dNTP pools are known players in the pathogenesis of multiple human diseases. Here we show that, even under physiological conditions, dGTP is largely overrepresented among other dNTPs in mitochondria of mouse tissues and human cultured cells. In addition, a vast majority of mitochondrial dGTP is tightly bound to NDUFA10, an accessory subunit of complex I of the mitochondrial respiratory chain. NDUFA10 shares a deoxyribonucleoside kinase (dNK) domain with deoxyribonucleoside kinases in the nucleotide salvage pathway, though no specific function beyond stabilizing the complex I holoenzyme has been described for this subunit. We mutated the dNK domain of NDUFA10 in human HEK-293T cells while preserving complex I assembly and activity. The NDUFA10E160A/R161A shows reduced dGTP binding capacity in vitro and leads to a 50% reduction in mitochondrial dGTP content, proving that most dGTP is directly bound to the dNK domain of NDUFA10. This interaction may represent a hitherto unknown mechanism regulating mitochondrial dNTP availability and linking oxidative metabolism to DNA maintenance

Bladder cancer index: cross-cultural adaptation into Spanish and psychometric evaluation

BACKGROUND: The Bladder Cancer Index (BCI) is so far the only instrument applicable across all bladder cancer patients, independent of tumor infiltration or treatment applied. We developed a Spanish version of the BCI, and assessed its acceptability and metric properties. METHODS: For the adaptation into Spanish we used the forward and back-translation method, expert panels, and cognitive debriefing patient interviews. For the assessment of metric properties we used data from 197 bladder cancer patients from a multi-center prospective study. The Spanish BCI and the SF-36 Health Survey were self-administered before and 12 months after treatment. Reliability was estimated by Cronbach's alpha. Construct validity was assessed through the multi-trait multi-method matrix. The magnitude of change was quantified by effect sizes to assess responsiveness. RESULTS: Reliability coefficients ranged 0.75-0.97. The validity analysis confirmed moderate associations between the BCI function and bother subscales for urinary (r = 0.61) and bowel (r = 0.53) domains; conceptual independence among all BCI domains (r ≤ 0.3); and low correlation coefficients with the SF-36 scores, ranging 0.14-0.48. Among patients reporting global improvement at follow-up, pre-post treatment changes were statistically significant for the urinary domain and urinary bother subscale, with effect sizes of 0.38 and 0.53. CONCLUSIONS: The Spanish BCI is well accepted, reliable, valid, responsive, and similar in performance compared to the original instrument. These findings support its use, both in Spanish and international studies, as a valuable and comprehensive tool for assessing quality of life across a wide range of bladder cancer patients

CIBERER : Spanish national network for research on rare diseases: A highly productive collaborative initiative

Altres ajuts: Instituto de Salud Carlos III (ISCIII); Ministerio de Ciencia e Innovación.CIBER (Center for Biomedical Network Research; Centro de Investigación Biomédica En Red) is a public national consortium created in 2006 under the umbrella of the Spanish National Institute of Health Carlos III (ISCIII). This innovative research structure comprises 11 different specific areas dedicated to the main public health priorities in the National Health System. CIBERER, the thematic area of CIBER focused on rare diseases (RDs) currently consists of 75 research groups belonging to universities, research centers, and hospitals of the entire country. CIBERER's mission is to be a center prioritizing and favoring collaboration and cooperation between biomedical and clinical research groups, with special emphasis on the aspects of genetic, molecular, biochemical, and cellular research of RDs. This research is the basis for providing new tools for the diagnosis and therapy of low-prevalence diseases, in line with the International Rare Diseases Research Consortium (IRDiRC) objectives, thus favoring translational research between the scientific environment of the laboratory and the clinical setting of health centers. In this article, we intend to review CIBERER's 15-year journey and summarize the main results obtained in terms of internationalization, scientific production, contributions toward the discovery of new therapies and novel genes associated to diseases, cooperation with patients' associations and many other topics related to RD research

Adelante / Endavant

Séptimo desafío por la erradicación de la violencia contra las mujeres del Institut Universitari d’Estudis Feministes i de Gènere "Purificación Escribano" de la Universitat Jaume

Factores socioeconómicos y frecuentación en las consultas de medicina de familia de la red sanitaria pública madrileña Socioeconomic factors and utilization of public family practice facilities in Madrid

Objetivos: Se describe la posible asociación entre las características demográficas y socioeconómicas de las áreas sanitarias de la Comunidad de Madrid y la frecuentación de las consultas de medicina de familia de atención primaria. Métodos: Estudio ecológico que utiliza el área sanitaria como unidad de análisis. Las fuentes de información fueron los datos oficiales sobre estadísticas de población y las memorias del Instituto Nacional de Salud de los años 1996 y 2001. Como indicadores se obtuvieron el nivel de renta, el tamaño medio del hogar, el paro, la población dedicada a las labores del hogar y la población con estudios superiores, analizándose la asociación con la frecuentación mediante el coeficiente de correlación de Spearman. Se ajustó un modelo multivariante de regresión lineal. Resultados: La frecuentación a la consulta de medicina de familia se asoció al porcentaje de personas dedicadas a las labores del hogar (r = 0,44) a la renta (r = -0,69) y a los estudios superiores (r = -0,72). El modelo multivariante mostró que la variabilidad de la frecuentación se explica en un 77,5% por la inclusión conjunta de la renta (un 48% de la variabilidad), el porcentaje de personas dedicadas a las labores del hogar (19%) y el tamaño del hogar (9%). Conclusiones: Estos resultados muestran la existencia de factores socioeconómicos que explican la utilización de las consultas de medicina de familia. Los resultados obtenidos aconsejan que la planificación de los servicios de atención primaria incorpore parámetros demográficos y socioeconómicos.<br>Objectives: To describe the possible association between demographic, social and economic characteristics of health areas in the autonomous community of Madrid and utilization of public family practice facilities. Methods: An ecological study was carried out using health areas as the unit of analysis. The information sources were official data on population statistics and the reports of the National Institute of Health for 1996 and 2001. Indicators were income, mean household size, unemployment rate, the percentage of housewives, and the percentage of individuals with university education. The association between these indicators and utilization of public family practice facilities was analyzed using the Spearman correlation coefficient. A multivariate linear regression model was also fitted. Results: The consultation rate in public family practice facilities in Madrid was directly associated with the percentage of housewives (r = 0.44), income (r = -0,697), and the percentage of individuals with university education (r = -0.72). In the multivariate linear regression model, 77% of the varian-ce in utilization was explained by income (48%), the percentage of housewives (19%), and average household size (9%). Conclusions: The results show the relationship between social and economic factors and utilization of public family practice facilities and suggest the advisability of including demographic and socioeconomic factors in primary care planning

Factores socioeconómicos y frecuentación en las consultas de medicina de familia de la red sanitaria pública madrileña

ResumenObjetivosSe describe la posible asociación entre las características demográficas y socioeconómicas de las áreas sanitarias de la Comunidad de Madrid y la frecuentación de las consultas de medicina de familia de atención primaria.MétodosEstudio ecológico que utiliza el área sanitaria como unidad de análisis. Las fuentes de información fueron los datos oficiales sobre estadísticas de población y las memorias del Instituto Nacional de Salud de los años 1996 y 2001. Como indicadores se obtuvieron el nivel de renta, el tamaño medio del hogar, el paro, la población dedicada a las labores del hogar y la población con estudios superiores, analizándose la asociación con la frecuentación mediante el coeficiente de correlación de Spearman. Se ajustó un modelo multivariante de regresión lineal.ResultadosLa frecuentación a la consulta de medicina de familia se asoció al porcentaje de personas dedicadas a las labores del hogar (r=0,44) a la renta (r=−0,69) y a los estudios superiores (r=−0,72). El modelo multivariante mostró que la variabilidad de la frecuentación se explica en un 77,5% por la inclusión conjunta de la renta (un 48% de la variabilidad), el porcentaje de personas dedicadas a las labores del hogar (19%) y el tamaño del hogar (9%).ConclusionesEstos resultados muestran la existencia de factores socioeconómicos que explican la utilización de las consultas de medicina de familia. Los resultados obtenidos aconsejan que la planificación de los servicios de atención primaria incorpore parámetros demográficos y socioeconómicos.AbstractObjectivesTo describe the possible association between demographic, social and economic characteristics of health areas in the autonomous community of Madrid and utilization of public family practice facilities.MethodsAn ecological study was carried out using health areas as the unit of analysis. The information sources were official data on population statistics and the reports of the National Institute of Health for 1996 and 2001. Indicators were income, mean household size, unemployment rate, the percentage of housewives, and the percentage of individuals with university education. The association between these indicators and utilization of public family practice facilities was analyzed using the Spearman correlation coefficient. A multivariate linear regression model was also fitted.ResultsThe consultation rate in public family practice facilities in Madrid was directly associated with the percentage of housewives (r=0.44), income (r=−0,697), and the percentage of individuals with university education (r=−0.72). In the multivariate linear regression model, 77% of the variance in utilization was explained by income (48%), the percentage of housewives (19%), and average household size (9%).ConclusionsThe results show the relationship between social and economic factors and utilization of public family practice facilities and suggest the advisability of including demographic and socioeconomic factors in primary care planning

Proyecto MINENERGYDESIGN: modelo de aplicación de la metodología Design Thinking en el aprendizaje en la gestión de proyectos de ingeniería = MINENERGYDESIGN project: Application model of the Design Thinking methodology in learning in the management of engineering projects

La gestión de proyectos en ingeniería se está transformando hacia un proceso dinámico y ágil, donde la interacción e iteración continua con el cliente/usuario es una realidad. Para el éxito del proyecto, y la resolución del problema de ingeniería, se deben considerar nuevas herramientas de aprendizaje, donde el alumno debe trabajar entre otras competencias, la creatividad (aplicada a la resolución de problemas), el trabajo en equipo, la comunicación y el liderazgo. El objetivo de este trabajo es mostrar cómo la metodología Design Thinking incrementa el aprendizaje en el área de gestión de proyectos proponiendo una sencilla iteración en tres etapas hasta alcanzar un prototipo funcional. De esta forma, el al umnado adquiere de una forma práctica las competencias demandadas por el entorno profesional, permitiendo tener una primera aproximación y experiencia en aula sobre la gestión de proyectos. ----------ABSTRACT---------- Project Management in engineering fields is being transformed towards an agile management, where the interaction with the client / user and iteration is a constant. For the success of the project, and the resolution of the engineering problem, new learning tools must be considered, where the student must work, among others, creativity competences (applied to problem solving), teamwork, communication and leadership. This paper shows how the Design Thinking methodology increases learning in the area of project management, in this case, proposing a simple iteration in three stages, until reaching a functional prototype. The students in this way acquire in a practical way the competences demanded by the professional environment, allowing to have a first approximation and classroom experience on the management of projects