1,671 research outputs found

    LEADERSHIP STYLE, POLICING AND PERCEPTION OF CORRUPTION: A COMPARATIVE PRELIMINARY STUDY WITHIN THE NIGERIA POLICE FORCE

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    The connection between leadership style, on the one hand, policing and perception of corruption, on the other hand, has received little attention in the literature on leadership theory and organizational processes. Hence, the aim of this study is to examine the relationship between transformational leadership and perception of corruption within the police institution, especially in this instance, the Nigeria Police Force. Based on a sampling process of cross sectional approach, the data were collected by administering questionnaires on 156 respondents in Alausa police district in Lagos State. The descriptive result shows that while transformational leadership is practiced in the Nigeria Police Force, the perceived level of corruption remains high. Though in varying degree, the results indicate a positive correlation between the attributes of transformational leadership (charisma, individualized consideration and intellectual stimulation) and the dimensions of corruption. While charisma consistently has a positive influence on the facets of corruption, individualized consideration and charisma influence the dimension of transparency within the police force. In addition, individualized consideration influences the facet of crime. Thus, transformational leadership seems to play an important, but complex role in the reduction of corrupt practices within the police institution. The findings were discussed in the specific contexts of transformational leadership theory, organizational practices and police corruption. The practical implications for organizational development, police reform and crusade against police deviances were also underscored

    Digestibilidade "in situ" da matéria seca da silagem de capim elefante (Pennisetum purpureum, Schum) com diferentes níveis de cunha (Clitoria ternatea, L).

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    Para as condições em que este trabalho foi conduzido, pode-se concluir que, A degradabilidade da matéria seca de todas as silagens foi em media 67,76%, caracterizando silagens de bom valor nutritivo

    Worldwide research trends on the use of chemical-mechanical caries removal products over the years: a critical review

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    [EN] Background Chemical¿mechanical caries removal (CMCR) products are in constant evolution and were recommended during the COVID-19 pandemic as substitutes for conventional caries removal. Aim Characterize the worldwide scientifc literature about CMCR products, over the years, by means of a critical review. Design Electronic search was performed on Medline/PubMed, Scopus, Web of Science, Cochrane Library, Lilacs, and Embase up to November 2020. Year, journal, country of authors, and type of study were the data extracted from the retrieved studies. Additional data of the clinical studies and systematic reviews were investigated. Results 2221 records were identifed, 397 selected. 2011¿2020 period concentrates higher number of publications (n=169), in the Journal of Dental Research (n=51), developed in Brazil (n=45) and India (n=44). Most studies were in vitro (n=211) and clinical trials (n=101). Carisolv¿ (n=48) and Papacarie Duo Gel¿ (n=33) were the most used products, prescript in isolated usage (n=101), and compared with drills (n=77). CMCR were more studied in primary teeth (n=78), receiving glass ionomer cement (GIC) (n=51) as restorative material. The most evaluated outcomes were time spent (n=48) and pain (n=41). Clinical application of CMCR takes more time than other techniques, but can also reduce patient anxiety, pain, and need for anesthesia. Conclusion In vitro and clinical studies with CMCR products have been increasing, mostly carried out in developing countries, evaluating Carisolv¿ and Papacarie Duo Gel¿. Clinical studies tend to evaluate the time spent and pain compared to drills for removing caries in primary teeth, posteriorly restored with GIC. CMCR clinical application reduces anxiety, pain, and need for anesthesia, despite increase treatments¿ time.This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brazil (CAPES) -Finance code 001, and Fundacao Carlos Chagas de Amparo a Pesquisa no Estado do Rio de Janeiro (FAPERJ)-Finance code E-26/202.766/2019, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) -Finance code 401058/2016-6 -for the VantagePointT software. This study is part of the Master's thesis of the primary author.Souza, TF.; Martins, ML.; Magno, MB.; Vicente Gomila, JM.; Fonseca-Goncalves, A.; Maia, LC. (2022). Worldwide research trends on the use of chemical-mechanical caries removal products over the years: a critical review. European Archives of Paediatric Dentistry. 23(6):869-883. https://doi.org/10.1007/s40368-022-00726-686988323

    Qualidade e conservação pós-colheita de tomates orgânicos produzidos sob diferentes sistemas de irrigação.

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    Suplemento. Trabalho apresentado no Congresso Brasileiro de Olericultura, 50., 2010, Guarapari. Cinquenta anos contribuindo para a saúde da população brasileira. Trabalho A2871-T4947

    Produção de uma xilanase fúngica por Komagataella phaffii em biorreator.

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    As xilanases são enzimas que hidrolisam ligações Beta-14 presentes na hemicelulose que compõe a parede celular vegetal. Essas enzimas são produzidas por bactérias, fungos e plantas. Por apresentarem diversas aplicações, como na fabricação de pães, na indústria farmacêutica, na fabricação de celulose e na geração de biocombustíveis, o objetivo desse trabalho foi escalonar a produção de uma xilanase de origem fúngica (F43 GH 10-03) por Komagataella phaffii em biorreatores para futura aplicação biotecnológica. Os cultivos foram realizados com fluxo de ar entre 0,1 a 4,0 vvm, pO2 fixado em 25%, os inóculos com densidade ótica a 600 nm iniciais fixadas em 2, 4 e 6 e temperatura de 30°C. Após fase de crescimento celular, foi estabelecida uma alimentação com glicose 50% (m/v) (fluxo de 4 g/L/h). Incialmente, a xilanase foi produzida em frascos apresentando atividade de 1,5 UI/mL entre 24 a 72 h de cultivo. A produção da xilanase em biorreator realizada em três condições com DO600 inicial fixada em 2, 4 e 6 mostrou que todos os cultivos apresentaram atividade de xilanase a partir de 14,5 h, com secreção de xilanase ascendente, com atividade variando entre 12 e 23 UI/mL, e a DO600 variando entre 197 e 234, com produção de proteínas totais entre 0,26 e 0,48 g/L. A análise por eletroforese mostrou uma banda de 55 kDa correspondendo à xilanase F43 GH 10-03. Dos três cultivos realizados, a estratégia com DO600 inicial fixada em 4 apresentou a maior atividade específica (70,7 UI/mg de proteína). Essa condição pode ser utilizada para o início de estudos de otimização de produção dessa enzima em biorreator

    Efeito do diâmetro do caule na alporquia em pequizeiro.

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    O potencial comercial do pequizeiro é ascendente, devido às características organolépticas marcantes do fruto que servem para uma infinidade de usos. A utilização do pequi vai muito além do uso humano, podendo ser utilizado na alimentação animal, sendo o farelo da casca do pequi uma alternativa viável para a alimentação de ruminantes. A propagação por sementes é comum em algumas espécies arbóreas devido à ausência de informações envolvendo outros métodos. Considerando as elevadas taxas de alogamia nas flores de pequizeiro, mudas produzidas de forma seminal apresentam grande variabilidade genética, o que acarreta enorme variabilidade fenotípica nos pomares. Para atenuar esses efeitos, a propagação via alporquia, que induz a formação de raízes adventícias em partes de caule ainda ligadas à planta mãe, apesar de mais trabalhosa, muitas vezes é mais eficiente na obtenção de mudas, a exemplo do que ocorre em pessegueiro (CASTRO e SILVEIRA, 2003). O objetivo do presente trabalho foi avaliar o efeito do diâmetro do caule e da planta matriz na sobrevivência, no calejamento e no enraizamento de alporques de pequizeiros nativos na região do Distrito Federal

    Multidrug-Resistant Nontuberculous Mycobacteria Isolated from Cystic Fibrosis Patients

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    Worldwide, nontuberculous mycobacteria (NTM) have become emergent pathogens of pulmonary infections in cystic fibrosis (CF) patients, with an estimated prevalence ranging from 5 to 20%. This work investigated the presence of NTM in sputum samples of 129 CF patients (2 to 18 years old) submitted to longitudinal clinical supervision at a regional reference center in Rio de Janeiro, Brazil. From June 2009 to March 2012, 36 NTM isolates recovered from 10 (7.75%) out of 129 children were obtained. Molecular identification of NTM was performed by using PCR restriction analysis targeting the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene, and susceptibility tests were performed that followed Clinical and Laboratory Standards Institute recommendations. for evaluating the genotypic diversity, pulsed-field gel electrophoresis (PFGE) and/or enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) was performed. the species identified were Mycobacterium abscessus subsp. bolletii (n = 24), M. abscessus subsp. abscessus (n = 6), Mycobacterium fortuitum (n = 3), Mycobacterium marseillense (n = 2), and Mycobacterium timonense (n = 1). Most of the isolates presented resistance to five or more of the antimicrobials tested. Typing profiles were mainly patient specific. the PFGE profiles indicated the presence of two clonal groups for M. abscessus subsp. abscessus and five clonal groups for M. abscesssus subsp. bolletii, with just one clone detected in two patients. Given the observed multidrug resistance patterns and the possibility of transmission between patients, we suggest the implementation of continuous and routine investigation of NTM infection or colonization in CF patients, including countries with a high burden of tuberculosis disease.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)PDTIS-FIOCRUZUniv Fed Rio de Janeiro, Programa Posgrad Clin Med, Hosp Univ Clementino Fraga Filho, Rio de Janeiro, BrazilUniv Fed Rio Grande do Sul, Programa Posgrad Ciencias Med, Porto Alegre, RS, BrazilUniv Fed Rio de Janeiro, Fac Ciencias Med, Dept Microbiol Imunol & Parasitol, Rio de Janeiro, BrazilInst Fernandes Figueira Fiocruz, Rio de Janeiro, BrazilUniv Estado Rio de Janeiro, Hosp Univ Pedro Ernesto, Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Microbiol, BR-21941 Rio de Janeiro, BrazilFundacao Oswaldo Cruz, Inst Pesquisa Evandro Chagas, Rio de Janeiro, BrazilInst Doencas Torax, Rio de Janeiro, BrazilJohns Hopkins Univ, Baltimore, MD USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv Fed Fluminense, Inst Biomed, Niteroi, RJ, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilFAPERJ: 103.225/2011FAPERJ: 103.287/2011FAPERJ: 110.272/2010FAPERJ: 110.761/2010FAPERJ: 111.497/2008CNPq: 476536/2012-0CNPq: 473444/2010-0CNPq: 567037/2008-8Web of Scienc

    Sialic Acid Glycobiology Unveils Trypanosoma cruzi Trypomastigote Membrane Physiology.

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    Trypanosoma cruzi, the flagellate protozoan agent of Chagas disease or American trypanosomiasis, is unable to synthesize sialic acids de novo. Mucins and trans-sialidase (TS) are substrate and enzyme, respectively, of the glycobiological system that scavenges sialic acid from the host in a crucial interplay for T. cruzi life cycle. The acquisition of the sialyl residue allows the parasite to avoid lysis by serum factors and to interact with the host cell. A major drawback to studying the sialylation kinetics and turnover of the trypomastigote glycoconjugates is the difficulty to identify and follow the recently acquired sialyl residues. To tackle this issue, we followed an unnatural sugar approach as bioorthogonal chemical reporters, where the use of azidosialyl residues allowed identifying the acquired sugar. Advanced microscopy techniques, together with biochemical methods, were used to study the trypomastigote membrane from its glycobiological perspective. Main sialyl acceptors were identified as mucins by biochemical procedures and protein markers. Together with determining their shedding and turnover rates, we also report that several membrane proteins, including TS and its substrates, both glycosylphosphatidylinositol-anchored proteins, are separately distributed on parasite surface and contained in different and highly stable membrane microdomains. Notably, labeling for α(1,3)Galactosyl residues only partially colocalize with sialylated mucins, indicating that two species of glycosylated mucins do exist, which are segregated at the parasite surface. Moreover, sialylated mucins were included in lipid-raft-domains, whereas TS molecules are not. The location of the surface-anchored TS resulted too far off as to be capable to sialylate mucins, a role played by the shed TS instead. Phosphatidylinositol-phospholipase-C activity is actually not present in trypomastigotes. Therefore, shedding of TS occurs via microvesicles instead of as a fully soluble form
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