Sentidos - projeto editorial de um livro de poemas de Nuno Guimarães

Mestrado em Estudos EditoriaisPretende-se com o presente relatório apresentar o projeto editorial do livro sentidos – um livro de poesia de Nuno Guimarães. sentidos é um livro para além do livro, na medida em que foi concebido tendo como principais motivações a admiração pela escrita poética de Nuno Guimarães e a temática dos sentidos. Um cd-audio com a locução da totalidade dos poemas, um livro de fotografias, o acesso privado a uma entrevista com o autor e o livro em si com um formato próprio, são características que fazem deste livro um artefacto artístico com características diferenciadoras e singulares.The intention of this report is to present the editorial project of Nuno Guimarães book - sentidos - a poetry book. sentidos is a book beyond the book as that it was done taking into account my admiration for Nuno Guimarães writings and the thematic of senses. An audio-cd (with the locution of all poems), a book of photographs, a private access to an interview with the author and the book itself, are aspects that make this an artistic artefact with unique and differentiating features

Enzymes and secondary metabolites profiles of Gnomoniopsis smithogilvyi are affected by chestnut medium

Chestnut tree (Castanea sativa Mill) is a crop with high economic and social importance in Trás-os-Montes Region. The pre and post-harvest quality of its nuts can be affected by many factors, being the lasses dueto fungi very large. During the last decade a new endophyte fungus, Gnomoniopsis smithogilvyi, causing brown rot, has been isolated from both rotten and healthy fruits, and its growth seems to be related with the increase of air temperatura and rainfall during spring. The present study aims to understand the influence of chestnut substrate on G. smithogilvyi biochemistry, in arder to develop strategies to reduce its incidence in the fruit and improve the contrai of brown rot. lnitially, the presence of the enzymes involved in the decomposition of chestnuts, such as amylase, proteases, carboxymethylcellulase (CMCase), ~-glucosidase, xylanase, among others, was investigated. Further, some hydrolase activities were evaluated in two isolates of G. smithogilvyi incubated in potato (PDB) and chestnut media (CM) along an incubation period (3, 7 and 14 days), in arder to study the effects "isolate", "medium" and "incubation period" on enzymes production. Also, the synthesis of secondary metabolites in fungai dry biomass was assessed by Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Ali isolates produced the enzymes screened and, in general, the enzyme production varied between the isolates (P< 0.0000), the media (P< 0.0000) and along the incubation period (P< 0.0000). Amylase activity was positively correlated with xylanase (P< 0.0001), CMCase (P< 0.0001), and Avicelase {P< 0.01), xylanase with CMCase (P< 0.01) and ~-glucosidase with Avicelase (P< 0.0001 ). By contrast, ~-glucosidase was negatively correlated with CMCase {P< 0.01 ). Twenty secondary metabolites were detected along the incubation: eight in both isolates and media, tive and three only in isolates grew in PD o r CM, respectively. Some of these metabolites had been associated with other fungi and have known biological activities.This work was suported by "ValorCast- Valorização da castanha e otimização da sua comercialização" (PDR2020-1.0.1- FEADER-032034}, funded by Fundo Europeu Agrfcola de Desenvolvimento Rural (FEADER} and the Portuguesa Government (Ação 1.1 Grupos Operacionais, Medida 1. Inovação, PDR 2020- Programa de Desenvolvimento Rural do Continente). AS, IF, JGL, AAD, are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CITAB (UIDB/04033/2020) and PR to CIMO (UIDB/00690/2020).info:eu-repo/semantics/publishedVersio

Horizon scanning in Brazil: outputs and repercussions

RESUMO OBJETIVO: Descrever os quatro tipos de produtos de monitoramento do horizonte tecnológico (MHT) elaborados no âmbito da Comissão Nacional de Incorporação de Tecnologias no Sistema Único de Saúde (Conitec) e apresentar suas principais repercussões nos processos de tomada de decisão do Ministério da Saúde brasileiro (MS). MÉTODOS: Estudo descritivo baseado em observação participante e análise documental dos produtos de MHT (relatórios internos, alertas, informes e seções para os relatórios de recomendação da Conitec) elaborados entre janeiro de 2014 e julho de 2018. RESULTADOS: Foram produzidos 15 relatórios internos, seis alertas, dois informes e 57 seções de MHT. Os produtos têm formatos diferentes, em especial conforme a finalidade. A abordagem metodológica adotada para o desenvolvimento dos produtos de MHT no Brasil está de acordo com o descrito pela EuroScan International Network. Observaram-se repercussões institucionais e internacional dos produtos. As atividades de MHT resultaram na inclusão do monitoramento de tecnologias novas e emergentes como ferramenta para a redução das ações judiciais em saúde no arcabouço legal do MS. Um dos relatórios internos sobre um medicamento de alto custo sem registro no Brasil para doença rara foi requerido pela Rede de Avaliação de Tecnologias em Saúde das Américas, demonstrando a relevância internacional dos produtos. As seções de MHT nos relatórios de recomendação influenciaram as discussões sobre a incorporação de tecnologias no Sistema Único de Saúde. CONCLUSÕES: Os produtos desenvolvidos apresentam finalidades que vão desde auxiliar na construção de argumentos para defesa do MS em casos de judicialização da saúde até informar processos para a tomada de decisão. Além disso, nos últimos anos as seções de MHT nos relatórios de recomendação têm apresentado crescente importância. O sistema de MHT vinculado à Conitec tem se estruturado, e seu papel como instrumento para informar gestores de saúde tem sido relevante.ABSTRACT OBJECTIVE: To describe the four types of horizon scanning (HS) outputs developed by the National Committee for Health Technology Incorporation (CONITEC) and show their main repercussions on the decision-making processes of the Brazilian Ministry of Health (MH). METHODS: Descriptive study based on participant observation and document analysis of HS outputs (internal reports, alert reports, briefs and sections for CONITEC recommendation reports) developed between January 2014 and July 2018. RESULTS: Fifteen internal reports, six alert reports, two briefs and 57 HS sections were produced. Each output has a specific structure according to its purpose. The methodological approach adopted for developing HS outputs in Brazil is described by EuroScan International Network. The outputs had institutional and international repercussions. The activities resulted in the inclusion of HS as a tool for reducing health lawsuits in the legal framework of the MH. One of the internal reports on a high-cost drug not approved in Brazil for a rare disease was requested by the Health Technology Assessments Network for the Americas (RedETSA), showing the international relevance of the outputs. The HS sections in recommendation reports influenced discussions about incorporating technologies into the Unified Health System. CONCLUSIONS: The developed outputs have purposes ranging from helping build arguments for defense of the MH in cases of health judicialization to inform decision-making processes. In addition, HS sections in recommendation reports have grown in importance recently. CONITEC’s HS system has been structured, and its role as a tool to inform health managers has shown to be been relevant

ReporTree: a surveillance-oriented tool to strengthen the linkage between pathogen genetic clusters and epidemiological data

OHEJP Project: BeONE https://doi.org/10.1186/s13073-023-01196-1 This repository contains the Open Access PDF file of the article: Mixão, V., Pinto, M., Sobral, D. et al. ReporTree: a surveillance-oriented tool to strengthen the linkage between pathogen genetic clusters and epidemiological data. Genome Med 15, 43 (2023). ----------------------------------- Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. ----------------------------------- Acknowledgements The authors thank Dr. Holger Brendebach, Dr. Carlus Deneke, and Dr. Simon Tausch from the German Federal Institute for Risk Assessment for their support during the genome assembly of the samples used in ReporTree benchmarking and Dr. João André Carriço for the productive discussions throughout ReporTree development. We would also like to thank the National Distributed Computing Infrastructure of Portugal (INCD) for providing the necessary resources to run the genome assemblies. INCD was funded by FCT and FEDER under the project 22153-01/SAICT/2016. Funding This work was supported by funding from the European Union’s Horizon 2020 Research and Innovation program under grant agreement No 773830: One Health European Joint Programme (2020–2022) and by national funds through FCT—Foundation for Science and Technology, I.P., in the frame of Individual CEEC 2022.00851.CEECIND/CP1748/CT0001 (2023 onwards)

Chestnut brown rot and Gnomoniopsis smithogilvyi: characterization of the disease and of the causal agent in Portugal

Sweet chestnut (Castanea sativa Mill.) is a nutritious food with high social and economic impact in Portugal. Fungal infection and rots lead to great economic and quality losses in stored chestnut. The fungus Gnomoniopsis smithogilvyi (syn. Gnomoniopsis castaneae) is currently considered one of the major threats to the chestnut chain worldwide. Considering the lack of knowledge on both the disease and the causal agent in Portugal, studies have been conducted in an attempt to timely develop the necessary control strategies towards the mitigation of the disease. Under this scope, the chestnut brown rot and its causal agent have been characterized for the first time in Portugal. The study was performed on chestnuts from Braganca, Portugal, that were received, processed and stored in a local industry during the growing season 2018-2019. Thirty-three samples were collected from different processing stages and from three chestnut varieties (Longal, Judia and Martaínha). Several isolates of G. smithogilvyi obtained were characterized at the morphological, ecophysiological, enzymatic and molecular levels. The fungus was also characterized in terms of pathogenicity and virulence. G. smithogilvyi was confirmed as the causal agent of brown rot disease in Portuguese chestnut varieties, which showed high susceptibility. The fungus showed high adaptability to chestnut substrates. The Portuguese isolates of G. smithogilvyi are morphologically and genetically similar to those isolated in other countries, even though some physiological and enzymatic variability was observed among the Portuguese isolates. Post-harvest control strategies towards the mitigation of the disease are discussed.This work was developed under the scope of “ValorCast – Valorização da castanha e otimização da sua comercialização” (PDR2020-1.0.1-FEADER-032034), funded by Fundo Europeu Agrícola de Desenvolvimento Rural (FEADER) and the Portuguese Government (Ação 1.1 Grupos Operacionais, Medida 1. Inovação, PDR 2020 – Programa de Desenvolvimento Rural do Continente). GP, JOD and PR are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020).info:eu-repo/semantics/publishedVersio

Demographic history of Canary Islands male gene-pool: replacement of native lineages by European

<p>Abstract</p> <p>Background</p> <p>The origin and prevalence of the prehispanic settlers of the Canary Islands has attracted great multidisciplinary interest. However, direct ancient DNA genetic studies on indigenous and historical 17^th–18^thcentury remains, using mitochondrial DNA as a female marker, have only recently been possible. In the present work, the analysis of Y-chromosome polymorphisms in the same samples, has shed light on the way the European colonization affected male and female Canary Island indigenous genetic pools, from the conquest to present-day times.</p> <p>Results</p> <p>Autochthonous (E-M81) and prominent (E-M78 and J-M267) Berber Y-chromosome lineages were detected in the indigenous remains, confirming a North West African origin for their ancestors which confirms previous mitochondrial DNA results. However, in contrast with their female lineages, which have survived in the present-day population since the conquest with only a moderate decline, the male indigenous lineages have dropped constantly being substituted by European lineages. Male and female sub-Saharan African genetic inputs were also detected in the Canary population, but their frequencies were higher during the 17^th–18^thcenturies than today.</p> <p>Conclusion</p> <p>The European colonization of the Canary Islands introduced a strong sex-biased change in the indigenous population in such a way that indigenous female lineages survived in the extant population in a significantly higher proportion than their male counterparts.</p

Complex interactions between p.His558Arg and linked variants in the sodium voltage-gated channel alpha subunit 5 (NaV1.5)

Common genetic polymorphisms may modify the phenotypic outcome when co-occurring with a disease-causing variant, and therefore understanding their modulating role in health and disease is of great importance. The polymorphic p.His558Arg variant of the sodium voltage-gated channel alpha subunit 5 (NaV1.5) encoded by the SCN5A gene is a case in point, as several studies have shown it can modify the clinical phenotype in a number of cardiac diseases. To evaluate the genetic backgrounds associated with this modulating effect, we reanalysed previous electrophysiological findings regarding the p.His558Arg variant and further assessed its patterns of genetic diversity in human populations. The NaV1.5 p.His558Arg variant was found to be in linkage disequilibrium with six other polymorphic variants that previously were also associated with cardiac traits in GWAS analyses. On account of this, incongruent reports that Arg558 allele can compensate, aggravate or have no effect on NaV1.5, likely might have arose due to a role of p.His558Arg depending on the additional linked variants. Altogether, these results indicate a major influence of the epistatic interactions between SCN5A variants, revealing also that phenotypic severity may depend on the polymorphic background associated to each individual genome.info:eu-repo/semantics/publishedVersio