2,097 research outputs found
Hábitos alimenticios de la rana bromélicola Phyllodytes edelmoi Peixoto, Caramaschi & Freire, 2003 (Anura: Hylidae) del Estado de Alagoas, Noreste de Brasil
El género de hílidos Phyllodytes está compuesto por 12 especies distribuidas en el este de Brasil y conocidas por habitar estrictamente bromeliáceas. En este estudio se exploran los hábitos alimenticios de P. edelmoi y se testea la selectividad de las presas por la especie comparando las presas encontradas en los estómagos con la disponibilidad de presas en las bromelias. Nuestros resultados muestran que P. edelmoi consume grandes cantidades de hormigas a lo largo del año, por lo que podría considerarse una especie "especializada en hormigas". Este comportamiento alimenticio especializado podría ser considerado una sinapomorfia para el género Phyllodytes.The hylid frog genus Phyllodytes comprised 12 species distributed in eastern Brazil and knownto strictly inhabit inside bromeliads. In this study, we explore the feeding habits of P. edelmoiand test the prey selectivity of the species by comparing the prey items in the stomachs contentswith the availability of preys in bromeliads. Our results show that P. edelmoi consumes highamounts of ants along the year, and therefore it could be considered an ?ant specialist? species.This specialist feeding behaviour could be considered a synapomorphy of the genus Phyllodytes.Fil: Araujo-Vieira, Katyuscia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; ArgentinaFil: Ubiratan G. Silva. Universidade Federal de Alagoas; BrasilFil: Jhonatan Guedes dos Santos. Universidade Federal de Alagoas; BrasilFil: Thame G. Ferreira. Departamento de Ecologia E Biomonitoramento; BrasilFil: Gabriel O. Skuk. Universidade Federal de Alagoas; Brasi
From Compact Discs to Streaming: A Comparison of Eras within the Brazilian Market
The music industry has undergone many changes in the last few decades, notably since vinyl, cassettes and compact discs faded away as streaming platforms took the world by storm. This Digital evolution has made huge volumes of data about music consumption available. Based on such data, we perform cross-era comparisons between Physical and Digital media within the music market in Brazil. First, we build artists' success time series to detect and characterize hot streak periods, defined as high-impact bursts that occur in sequence, in both eras. Then, we identify groups of artists with distinct success levels by applying a cluster analysis based on hot streaks' features. We find the same clusters for both Physical and Digital eras: Spike Hit Artists, Big Hit Artists, and Top Hit Artists. Our results reveal significant changes in the music industry dynamics over the years by identifying the core of each era
the complexity of host's effective immune response against a polymorphic parasitic disease
This review is aimed at providing a comprehensive outline of the immune response displayed against cutaneous leishmaniasis (CL), the more common zoonotic infection caused by protozoan parasites of the genus Leishmania. Although of polymorphic clinical presentation, classically CL is characterized by leishmaniotic lesions on the face and extremities of the patients, which can be ulcerative, and even after healing can lead to permanent injuries and disfigurement, affecting significantly their psychological, social, and economic well-being. According a report released by the World Health Organization, the disability-adjusted life years (DALYs) lost due to leishmaniasis are close to 2.4 million, annually there are 1.0-1.5 million new cases of CL, and a numerous population is at risk in the endemic areas. Despite its increasing worldwide incidence, it is one of the so-called neglected tropical diseases. Furthermore, this review provides an overview of the existing knowledge of the host innate and acquired immune response to cutaneous species of Leishmania. The use of animal models and of in vitro studies has improved the understanding of parasite-host interplay and the complexity of immune mechanisms involved. The importance of diagnosis accuracy associated with effective patient management in CL reduction is highlighted. However, the multiple factors involved in CL epizoology associated with the unavailability of vaccines or drugs to prevent infection make difficult to formulate an effective strategy for CL control.publishersversionpublishe
Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters
Insertion specificity of mobile genetic elements is a rather complex aspect of DNA transposition, which, despite much progress towards its elucidation, still remains incompletely understood. We report here the results of a meta-analysis of IS2 target sites from genomic, phage, and plasmid DNA and find that newly acquired IS2 elements are consistently inserted around abrupt DNA compositional shifts, particularly in the form of switch sites of GC skew. The results presented in this study not only corroborate our previous observations that both the insertion sequence (IS) minicircle junction and target region adopt intrinsically bent conformations in IS2, but most interestingly, extend this requirement to other families of IS elements. Using this information, we were able to pinpoint regions with high propensity for transposition and to predict and detect, de novo, a novel IS2 insertion event in the 3′ region of the gfp gene of a reporter plasmid. We also found that during amplification of this plasmid, process parameters such as scale, culture growth phase, and medium composition exacerbate IS2 transposition, leading to contamination levels with potentially detrimental clinical effects. Overall, our findings provide new insights into the role of target DNA structure in the mechanism of transposition of IS elements and extend our understanding of how culture conditions are a relevant factor in the induction of genetic instability.Fundação para a Ciência e a Tecnologia (PTDC/EBB-EBI/113650/2009)MIT-Portugal Progra
Enterotype May Drive the Dietary-Associated Cardiometabolic Risk Factors
Analyses of typical bacterial clusters in humans named enterotypes may facilitate understanding the host differences in the cardiometabolic profile. It stills unknown whether the three previously described enterotypes were present in populations living below the equator. We examined how the identification of enterotypes could be useful to explain the dietary associations with cardiometabolic risk factors in Brazilian subjects. In this cross-sectional study, a convenience sample of 268 adults (54.2% women) reported their dietary habits and had clinical and biological samples collected. In this study, we analyzed biochemical data and metagenomics of fecal microbiota (16SrRNA sequencing, V4 region). Continuous variables were compared using ANOVA, and categorical variables using chi-square test. Vsearch clustered the operational taxonomic units, and Silva Database provided the taxonomic signatures. Spearman coefficient was used to verify the correlation between bacteria abundances within each enterotype. One hundred subjects were classified as omnivore, 102 lacto-ovo-vegetarians, and 66 strict vegetarians. We found the same structure as the three previously described enterotypes: 111 participants were assigned to Bacteroides, 55 to Prevotella, and 102 to Ruminococcaceae enterotype. The Prevotella cluster contained higher amount of strict vegetarians individuals than the other enterotypes (40.0 vs. 20.7 and 20.6, p = 0.04). Subjects in this enterotype had a similar anthropometric profile but a lower mean LDL-c concentration than the Bacteroides enterotype (96 +/- 23 vs. 109 +/- 32 mg/dL, p = 0.04). We observed significant correlations between bacterial abundances and cardiometabolic risk factors, but coefficients differed depending on the enterotype. In Prevotella enterotype, Eubacterium ventriosum (r BMI = -0.33, p = 0.03, and r HDL-c = 0.33, p = 0.04), Akkermansia (r 2h glucose = -0.35, p = 0.02), Roseburia (r BMI = -0.36, p = 0.02 and r waist = -0.36, p = 0.02), and Faecalibacterium (r insulin = -0.35, p = 0.02) abundances were associated to better cardiometabolic profile. The three enterotypes previously described are present in Brazilians, supporting that those bacterial clusters are not population-specific. Diet-independent lower LDL-c levels in subjects from Prevotella than in other enterotypes suggest that a protective bacterial cluster in the former should be driving this association. Enterotypes seem to be useful to understand the impact of daily diet exposure on cardiometabolic risk factors. Prospective studies are needed to confirm their utility for predicting phenotypes in humans.FAPESPUniv Sao Paulo, Sch Publ Hlth, Dept Epidemiol, Sao Paulo, BrazilFundacao Oswaldo Cruz, Rene Rachou Res Ctr, Belo Horizonte, MG, BrazilUniv Fed Sao Paulo, Dept Prevent Med, Sao Paulo, BrazilUniv Sao Paulo, Sch Med, Heart Inst Incor, Lab Genet & Mol Cardiol, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Prevent Med, Sao Paulo, BrazilFAPESP: 2012/12626-9FAPESP: 2012/03880-9Web of Scienc
Maternal Melatonin Programs the Daily Pattern of Energy Metabolism in Adult Offspring
Background: Shift work was recently described as a factor that increases the risk of Type 2 diabetes mellitus. In addition, rats born to mothers subjected to a phase shift throughout pregnancy are glucose intolerant. However, the mechanism by which a phase shift transmits metabolic information to the offspring has not been determined. Among several endocrine secretions, phase shifts in the light/dark cycle were described as altering the circadian profile of melatonin production by the pineal gland. The present study addresses the importance of maternal melatonin for the metabolic programming of the offspring. Methodology/Principal Findings: Female Wistar rats were submitted to SHAM surgery or pinealectomy (PINX). The PINX rats were divided into two groups and received either melatonin (PM) or vehicle. The SHAM, the PINX vehicle and the PM females were housed with male Wistar rats. Rats were allowed to mate and after weaning, the male and female offspring were subjected to a glucose tolerance test (GTT), a pyruvate tolerance test (PTT) and an insulin tolerance test (ITT). Pancreatic islets were isolated for insulin secretion, and insulin signaling was assessed in the liver and in the skeletal muscle by western blots. We found that male and female rats born to PINX mothers display glucose intolerance at the end of the light phase of the light/dark cycle, but not at the beginning. We further demonstrate that impaired glucose-stimulated insulin secretion and hepatic insulin resistance are mechanisms that may contribute to glucose intolerance in the offspring of PINX mothers. The metabolic programming described here occurs due to an absence of maternal melatonin because the offspring born to PINX mothers treated with melatonin were not glucose intolerant. Conclusions/Significance: The present results support the novel concept that maternal melatonin is responsible for the programming of the daily pattern of energy metabolism in their offspring.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)CNPq (Conselho Nacional de Aperfeicoameno Cientifico)CNPq (Conselho Nacional de Aperfeicoameno Cientifico
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