Drying dip-coated colloidal films

We present the results from a small-angle X-ray scattering (SAXS) study of lateral drying in thin films. The films, initially 10 μm thick, are cast by dip-coating a mica sheet in an aqueous silica dispersion (particle radius 8 nm, volume fraction ϕs = 0.14). During evaporation, a drying front sweeps across the film. An X-ray beam is focused on a selected spot of the film, and SAXS patterns are recorded at regular time intervals. As the film evaporates, SAXS spectra measure the ordering of particles, their volume fraction, the film thickness, and the water content, and a video camera images the solid regions of the film, recognized through their scattering of light. We find that the colloidal dispersion is first concentrated to ϕs = 0.3, where the silica particles begin to jam under the effect of their repulsive interactions. Then the particles aggregate until they form a cohesive wet solid at ϕs = 0.68 ± 0.02. Further evaporation from the wet solid leads to evacuation of water from pores of the film but leaves a residual water fraction ϕw = 0.16. The whole drying process is completed within 3 min. An important finding is that, in any spot (away from boundaries), the number of particles is conserved throughout this drying process, leading to the formation of a homogeneous deposit. This implies that no flow of particles occurs in our films during drying, a behavior distinct to that encountered in the iconic coffee-stain drying. It is argued that this type of evolution is associated with the formation of a transition region that propagates ahead of the drying front. In this region the gradient of osmotic pressure balances the drag force exerted on the particles by capillary flow toward the liquid–solid front

Formation of shear bands in drying colloidal dispersions

In directionally dried colloidal dispersions regular bands can appear behind the drying front, inclined at ±45° to the drying line. Although these features have been noted to share visual similarities with shear bands in metal, no physical mechanism for their formation has ever been suggested, until very recently. Here, through microscopy of silica and polystyrene dispersions, dried in Hele-Shaw cells, we demonstrate that the bands are indeed associated with local shear strains. We further show how the bands form, that they scale with the thickness of the drying layer, and that they are eliminated by the addition of salt to the drying dispersions. Finally, we reveal the origins of these bands in the compressive forces associated with drying

A structural systems biology approach for quantifying the systemic consequences of missense mutations in proteins

Gauging the systemic effects of non-synonymous single nucleotide polymorphisms (nsSNPs) is an important topic in the pursuit of personalized medicine. However, it is a non-trivial task to understand how a change at the protein structure level eventually affects a cell's behavior. This is because complex information at both the protein and pathway level has to be integrated. Given that the idea of integrating both protein and pathway dynamics to estimate the systemic impact of missense mutations in proteins remains predominantly unexplored, we investigate the practicality of such an approach by formulating mathematical models and comparing them with experimental data to study missense mutations. We present two case studies: (1) interpreting systemic perturbation for mutations within the cell cycle control mechanisms (G2 to mitosis transition) for yeast; (2) phenotypic classification of neuron-related human diseases associated with mutations within the mitogen-activated protein kinase (MAPK) pathway. We show that the application of simplified mathematical models is feasible for understanding the effects of small sequence changes on cellular behavior. Furthermore, we show that the systemic impact of missense mutations can be effectively quantified as a combination of protein stability change and pathway perturbation

Hiding in Plain View: Colloidal Self-Assembly from Polydisperse Populations

We report small-angle x-ray scattering experiments on aqueous dispersions of colloidal silica with a broad monomodal size distribution (polydispersity, 14%; size, 8 nm). Over a range of volume fractions, the silica particles segregate to build first one, then two distinct sets of colloidal crystals. These dispersions thus demonstrate fractional crystallization and multiple-phase (bcc, Laves AB2, liquid) coexistence. Their remarkable ability to build complex crystal structures from a polydisperse population originates from the intermediate-range nature of interparticle forces, and it suggests routes for designing self-assembling colloidal crystals from the bottom up

Factors Affecting Weaning Weight Production

Weaning weight records from a commercial cow herd were statistically analyzed to demonstrate suspected sources of variation in weaning weight. Data from first-calf heifers and mature cows (3 to 9 year olds) were analyzed separately. For every one day increase in calf age at weaning, weaning weight was increased by 1.65 and 1.76 Ib for first-calf heifers and mature cows, respectively. For every 1 percentage point increase in MPPA of the cow, weaning weight was increased by 4.5 Ib. A 10% advantage in weaning weights and weight per day of age (WDA) was observed in steers over heifers. A 3 and 6% advantage in WDA and weaning weight, respectively, was observed in bulls over steers. Weaning weight and WDA peaked among cows 4 to 6 years of age and then declined. Cows that were 3, 8 and 9 years of age produced calves with similar weaning weights. Calves nursing 3-, 7- and 8-year-old cows required more treatments for sickness than calves nursing 4- to 6-year-old cows. Production records provide valuable insight into causes of variation in cow productivity

Packing polydisperse colloids into crystals: when charge-dispersity matters

Monte Carlo simulations, fully constrained by experimental parameters, are found to agree well with a measured phase diagram of aqueous dispersions of nanoparticles with a moderate size polydispersity over a broad range of salt concentrations and volume fractions. Upon increasing volume fraction the colloids freeze first into coexisting compact solids then into a body centered cubic phase (bcc) before they melt into a glass forming liquid. The surprising stability of the bcc solid at high volume fractions and salt concentrations is explained by the interaction (charge) polydispersity and vibrational entropy

Effect of Bull Exposure on Reproductive Performance of First-Calf Heifers Bred by Natrual Service

Ninety-four spring calving, first-calf heifers were allotted to one of two treatment groups, control or bull exposed, to study the effect of bull exposure early postpartum on rebreeding performance when bred by natural service. Bull exposure early postpartum had no effect on pregnancy rate. However, bull exposure unexpectedly increased the subsequent calving interval by 9 days (P\u3c.05) and delayed the average calving date by 7 days (P\u3c.05). Under the conditions of this study in which heifers were in condition score 5 or better at calving and bred by natural service, bull exposure early postpartum did not improve reproductive performance

South Dakota Retained Ownership Demonstration

Four hundred nineteen steer calves representing 57 cow-calf producers were consigned to a custom feedlot in mid-October. Cattle were fed in one of three pens. One pen of calves was fed a starter program for 20 days followed by a moderate roughage growing diet for 84 days before they were switched to a high energy finishing diet (TWO). The other two pens were fed a starter program for 20 days followed by a moderate roughage growing diet for 14 days before they were switched to a high energy finishing diet. Cattle were sorted into one of these two pens on the basis of whether they had been exposed to feed (AFED, either weaned or creep fed) prior to feedlot arrival or not exposed to feed (ANFED). The TWO calves weighed 500 1b initially, gained 2.80 Ib per head daily, and averaged 1047 1b at slaughter after an average of 196 days on feed. Average cost of gain and profitability were $58.27 per cwt and$28.74 per head, respectively. The AFED and ANFED calves weighed 539 and 554 Ib initially, gained 3.04 and 3.08 Ib per head daily, and averaged 11 16 and 1136 1b at slaughter after an average of 190 and 189 days on feed, respectively. Average cost of gain and profitability were $55.40 and$56.32 per cwt and $23.57 and$33.20 per head, respectively. When data from years 1 and 2 were combined, average daily gain, dressing percentage, quality grade, and cost of gain were related to profitability and accounted for 79.6% of the variation in profitability

Surface-sensitive NMR in optically pumped semiconductors

We present a scheme of surface-sensitive nuclear magnetic resonance in optically pumped semiconductors, where an NMR signal from a part of the surface of a bulk compound semiconductor is detected apart from the bulk signal. It utilizes optically oriented nuclei with a long spin-lattice relaxation time as a polarization reservoir for the second (target) nuclei to be detected. It provides a basis for the nuclear spin polarizer [IEEE Trans. Appl. Supercond. 14, 1635 (2004)], which is a polarization reservoir at a surface of the optically pumped semiconductor that polarizes nuclear spins in a target material in contact through the nanostructured interfaces.Comment: 4 pages, 5 figure

Offshore-onshore record of Last Glacial Maximum-to-present grounding line retreat at Pine Island Glacier, Antarctica

Pine Island Glacier, West Antarctica, is the largest Antarctic contributor to global sea-level rise and is vulnerable to rapid retreat, yet our knowledge of its deglacial history since the Last Glacial Maximum is based largely on marine sediments that record retreat to ~120 km downstream of the modern grounding line by the early Holocene. We show, with a suite of 10Be exposure ages from onshore glacial deposits directly adjacent to Pine Island Glacier, that this major glacier thinned rapidly in the early- to mid-Holocene. Our results indicate that Pine Island Glacier was at least 690 m thicker than present prior to ~8 ka. We infer that the rapid thinning detected at the site furthest downstream records the arrival and stabilization of the retreating grounding line at that site by 8-6 ka. By combining our exposure ages and the marine record, we extend knowledge of Pine Island Glacier retreat both spatially and temporally: to 50 km from the modern grounding line and to the mid-Holocene, providing a dataset that is important for future numerical ice sheet model validation