Part of panel for exhibition opening Film Premiere and Q&A; Co-authored exhibition entry Marble Pavilion: for education and research

Part of Exhibition Opening Film Premiere and Q&A. Plus co-author of exhibition case study Marble Pavilion; for education and research. Case Study: Collaborative design an construction of a non-reinforced masonry structure using waste marble. In the town of Vila Viçosa, Portugal, where everything from castles to kerbstones are made out of marble, our pavilion is the first non-reinforced marble structure for over a century, and the first to be exclusively built from marble waste and with this type of structural system. The design research has from the beginning been integrated into undergraduate teaching. Students have lead particular lines of enquiries and collectively worked directly with the Quarries, Marble factory, Geologists and the community. We are inherently motivated by culture. Methodical knowledge exchange year on year, critical reflection, debate and further experimentation are the core components to the research lead teaching and praxis. (Patti Lather1986 Research as Praxis) (Venturi & Scott Brown, Yale, 1969). Leading to the design and construction of our first architectural scale experiment. The pavilion is fabricated and installed in Etma a Portuguese marble factory with Marble from Solubema. The pavilion’s intended use post construction is as an educational tool for employees, local students, international architects, engineers, clients and not least for our ongoing research. The structure, at only 2-3cm thick and comprising of just two layers of laminated marble, springs from one curved wall before forming two sweeping catenary vaults

Hypertrophic cardiomyopathy in young Maine Coon cats caused by the p.A31P cMyBP-C mutation - the clinical significance of having the mutation

<p>Abstract</p> <p>Background</p> <p>In Maine Coon (MC) cats the c.91G > C mutation in the gene <it>MYBPC3</it>, coding for cardiac myosin binding protein C (cMyBP-C), is associated with feline hypertrophic cardiomyopathy (fHCM). The mutation causes a substitution of an alanine for a proline at residue 31 (p.A31P) of cMyBP-C. The pattern of inheritance has been considered autosomal dominant based on a single pedigree. However, larger studies are needed to establish the significance of cats being heterozygous or homozygous for the mutation with respect to echocardiographic indices and the probability of developing fHCM. The objective of the present study was to establish the clinical significance of being homozygous or heterozygous for the p.A31P cMyBP-C mutation in young to middle-aged cats.</p> <p>Methods</p> <p>The cohort consisted of 332 MC cats, 282 cats < 4 years (85%). All cats were examined by 2-D and M-mode echocardiography. DNA was extracted from blood samples or buccal swabs and screened for the p.A31P cMyBP-C mutation in exon 3 of the gene, using polymerase chain reaction followed by DNA sequencing.</p> <p>Results</p> <p>The fHCM prevalence was 6.3% in the cohort. Eighteen cats were homozygous and 89 cats were heterozygous for the mutation. The odds ratio for having fHCM for homozygous cats was 21.6 (95% confidence interval 7.01-66.2) - when the group of equivocal cats was categorized as non-affected. Overall, 50% of the cats that were homozygous for the mutation had fHCM. p.A31P heterozygosity was not associated with a significant odds ratio for fHCM. In cats in the 4 to 6 years of age range a similar, non significant, odds ratio was seen in heterozygous cats. Only two cats over four years were homozygous and both were diagnosed with fHCM.</p> <p>Conclusion</p> <p>As there is no significant odds ratio associated with being heterozygous for the pA31P cMyBP-C mutation at this age, the mutation must have a very low penetrance in this group. From our data it would appear that most MC cats that develop fHCM due to the p.A31P mutation prior to the age of approximately 6 years do so because they are homozygous for this mutation.</p

En ny kirkegårdsvandringved Lild kirke i Hanherred

Intet resumé

Effects of temperature on tissue-diet isotopic spacing of nitrogen and carbon in otolith organic matter

Reconstruction of the trophic position of a fish can be performed by analysing stable nitrogen and carbon isotopes in otolith protein. However, ambient temperature may affect the tissue–diet isotopic spacing of stable isotopes from diet to predator tissue and bias estimates of trophic position. To test this, otolith protein, heart and muscle tissue from a rearing experiment with juvenile cod held at different temperatures (4, 7, 10 and 14°C) were analysed. There was no significant effect of temperature on otolith δ15N, whereas muscle and heart exhibited a slight decrease in δ15N values with increasing temperature corresponding to maximum of 0.6‰ over the 10°C range. By contrast, the otolith protein δ13C values at 4 and 7°C were significantly higher than for 10 and 14°C, suggesting an approximate 1‰ increased tissue–diet enrichment at the lower temperatures. Temperature had no significant effect on muscle and heart δ13C values. Considering the annual mean variation in ocean temperatures, our results indicate that the trophic signals recorded in the otoliths will reflect changes in diet isotope values with little bias from the ambient temperature experienced by the fish.publishedVersio

Otolith δ13C values as a metabolic proxy: Approaches and mechanical underpinnings

Knowledge of metabolic costs associated with maintenance, foraging, activity and growth under natural conditions is important for understanding fish behaviours and the bioenergetic consequences of a changing environment. Fish performance in the wild and within a complex environment can be investigated by analysing individual-level field metabolic rate and, at present, the natural stable carbon isotope tracer in otoliths offers the possibility to reconstruct field metabolic rate. The isotopic composition of carbon in fish otoliths is linked to oxygen consumption through metabolic oxidation of dietary carbon. The proportion of metabolically derived carbon can be estimated with knowledge of δ13C values of diet and dissolved inorganic carbon in the water. Over the past 10 years, new techniques to study fish ecology have been developed, and these can be used to strengthen the application of otolith δ13C values as a metabolic proxy. Here, we illustrate the great potential of the otolith δ13C metabolic proxy in combination with other valuable and well-established approaches. The novel approach of the otolith δ13C metabolic proxy allows us to track the effects of ontogenetic and environmental drivers on individual fish physiology, and removes a major obstacle to understanding and predicting the performance of free-ranging wild fish.publishedVersio

Application of CryoSat-2 altimetry data for river analysis and modelling

Availability of in situ river monitoring data, especially of data shared across boundaries, is decreasing, despite growing challenges for water resource management across the entire globe. This is especially valid for the case study of this work, the Brahmaputra Basin in South Asia. Commonly, satellite altimeters are used in various ways to provide information about such river basins. Most missions provide virtual station time series of water levels at locations where their repeat orbits cross rivers. CryoSat-2 is equipped with a new type of altimeter, providing estimates of the actual ground location seen in the reflected signal. It also uses a drifting orbit, challenging conventional ways of processing altimetry data to river water levels and their incorporation in hydrologic&ndash;hydrodynamic models. However, CryoSat-2 altimetry data provides an unprecedentedly high spatial resolution. This paper suggests a procedure to (i) filter CryoSat-2 observations over rivers to extract water-level profiles along the river, and (ii) use this information in combination with a hydrologic&ndash;hydrodynamic model to fit the simulated water levels with an accuracy that cannot be reached using information from globally available digital elevation models (DEMs) such as from the Shuttle Radar Topography Mission (SRTM) only. The filtering was done based on dynamic river masks extracted from Landsat imagery, providing spatial and temporal resolutions high enough to map the braided river channels and their dynamic morphology. This allowed extraction of river water levels over previously unmonitored narrow stretches of the river. In the Assam Valley section of the Brahmaputra River, CryoSat-2 data and Envisat virtual station data were combined to calibrate cross sections in a 1-D hydrodynamic model of the river. The hydrologic&ndash;hydrodynamic model setup and calibration are almost exclusively based on openly available remote sensing data and other global data sources, ensuring transferability of the developed methods. They provide an opportunity to achieve forecasts of both discharge and water levels in a poorly gauged river system

A century of fish growth in relation to climate change, population dynamics and exploitation

Marine ecosystems, particularly in high‐latitude regions such as the Arctic, have been significantly affected by human activities and contributions to climate change. Evaluating how fish populations responded to past changes in their environment is helpful for evaluating their future patterns, but is often hindered by the lack of long‐term biological data available. Using otolith increments of Northeast Arctic cod (Gadus morhua) as a proxy for individual growth, we developed a century‐scale biochronology (1924–2014) based on the measurements of 3,894 fish, which revealed significant variations in cod growth over the last 91 years. We combined mixed‐effect modeling and path analysis to relate these growth variations to selected climate, population and fishing‐related factors. Cod growth was negatively related to cod population size and positively related to capelin population size, one of the most important prey items. This suggests that density‐dependent effects are the main source of growth variability due to competition for resources and cannibalism. Growth was also positively correlated with warming sea temperatures but negatively correlated with the Atlantic Multidecadal Oscillation, suggesting contrasting effects of climate warming at different spatial scales. Fishing pressure had a significant but weak negative direct impact on growth. Additionally, path analysis revealed that the selected growth factors were interrelated. Capelin biomass was positively related to sea temperature and negatively influenced by herring biomass, while cod biomass was mainly driven by fishing mortality. Together, these results give a better understanding of how multiple interacting factors have shaped cod growth throughout a century, both directly and indirectly.publishedVersio