58 research outputs found
Optical doping and damage formation in AIN by Eu implantation
AlN films grown on sapphire were implanted with 300 keV Eu ions to fluences from 3×1014 to 1.4×1017 atoms/cm2 in two different geometries: “channeled” along the c-axis and “random” with a 10° angle between the ion beam and the surface normal. A detailed study of implantation damage accumulation is presented. Strong ion channeling effects are observed leading to significantly decreased damage levels for the channeled implantation within the entire fluence range. For random implantation, a buried amorphous layer is formed at the highest fluences. Red Eu-related photoluminescence at room temperature is observed in all samples with highest intensities for low damage samples (low fluence and channeled implantation) after annealing. Implantation damage, once formed, is shown to be stable up to very high temperatures.FCT - POCI/FIS/57550/2004FCT - PTDC/FIS/66262/2006FCT - PTDC/CTM/100756/200
Metabolic Reconstruction for Metagenomic Data and Its Application to the Human Microbiome
Microbial communities carry out the majority of the biochemical activity on the planet, and they play integral roles in processes including metabolism and immune homeostasis in the human microbiome. Shotgun sequencing of such communities' metagenomes provides information complementary to organismal abundances from taxonomic markers, but the resulting data typically comprise short reads from hundreds of different organisms and are at best challenging to assemble comparably to single-organism genomes. Here, we describe an alternative approach to infer the functional and metabolic potential of a microbial community metagenome. We determined the gene families and pathways present or absent within a community, as well as their relative abundances, directly from short sequence reads. We validated this methodology using a collection of synthetic metagenomes, recovering the presence and abundance both of large pathways and of small functional modules with high accuracy. We subsequently applied this method, HUMAnN, to the microbial communities of 649 metagenomes drawn from seven primary body sites on 102 individuals as part of the Human Microbiome Project (HMP). This provided a means to compare functional diversity and organismal ecology in the human microbiome, and we determined a core of 24 ubiquitously present modules. Core pathways were often implemented by different enzyme families within different body sites, and 168 functional modules and 196 metabolic pathways varied in metagenomic abundance specifically to one or more niches within the microbiome. These included glycosaminoglycan degradation in the gut, as well as phosphate and amino acid transport linked to host phenotype (vaginal pH) in the posterior fornix. An implementation of our methodology is available at http://huttenhower.sph.harvard.edu/humann. This provides a means to accurately and efficiently characterize microbial metabolic pathways and functional modules directly from high-throughput sequencing reads, enabling the determination of community roles in the HMP cohort and in future metagenomic studies.National Institutes of Health (U.S.) (U54HG004968
Taking the diet of cows into consideration in designing payments to reduce enteric methane emissions on dairy farms
ABSTRACT: Enteric fermentation from dairy cows is a major source of methane. Significantly and rapidly reducing those emissions would be a powerful lever to mitigate climate change. For a given productivity level, introducing fodder with high sources of n-3 content, such as grass or linseed, in the feed ration of dairy cows both improves the milk nutritional profile and reduces enteric methane emissions per liter. Changing cows' diet may represent additional costs for dairy farmers and calls for the implementation of payments for environmental services to support the transition. This paper analyzes 2 design elements influencing the effectiveness of a payment conditioned toward the reduction of enteric methane emissions: (1) the choice of emission indicator capturing the effect of farmers' practices, and (2) the payment amount relative to the additional milk production costs incurred. Using representative farm-level economic data from the French farm accountancy data network, we compare enteric methane emissions per liter of milk calculated with an Intergovernmental Panel on Climate Change Tier 2 method, to baseline emissions from a Tier 3 method accounting for diet effects. We also quantify the additional milk production costs of integrating more grass in the fodder systems by estimating variable cost functions for different dairy systems in France. Our results show the relevance of using an emission indicator sensitive to diet effects, and that the significance and direction of the additional costs for producing milk with a diet containing more grass differ according to the production basin and the current share of grasslands in the fodder crop rotation. We emphasize the importance of developing payments for environmental services with well-defined environmental indicators accounting for the technical problems addressed, and the need to better characterize heterogeneous funding requirements for supporting a large-scale adoption of more environment-friendly practices by farmers
Rare Earth Ion Implantation in GaN: Damage Formation and Recovery
Rare earth ions implanted GaN has been investigated by transmission electron microscopy versus the fluence, using Er, Eu or Tm ions at 150 keV or 300 keV and at room temperature. Point defect clusters and stacking faults are generated from low fluences (7×10 at/cm), their density increases with the fluence up to the formation of a highly disordered layer at the surface. This highly disordered layer is observed from a threshold fluence of 3×10 at/cm at 150 keV and 3×10 at/cm at 300 keV, and appears to be composed of voids and misoriented nanocrystallites. Its thickness rapidly increases with the fluence, and then saturates. Both basal and prismatic stacking faults were observed. Basal stacking faults are I in majority, but E or I have also been identified. I basal stacking faults propagate easily through GaN by folding from basal to prismatic planes. Channelling implantation, increasing the implantation temperature from room temperature to 500ºC, or implanting through a 10 nm thick AlN cap reduce the crystallographic damage, particularly by retarding the formation of the highly disordered layer. Implanting through the AlN cap allows the highly disordered layer formation threshold fluence to be increased by one order of magnitude, as well as the annealing at high temperature (1300ºC) which brings about a strong optical activation of the rare earths
A European perspective on acceptability of innovative agri-environment-climate contract solutions
The agri-environment-climate measures of the European Union Common Agricultural Policy are incentives aiming to reduce negative environmental impacts and increase positive effects generated from agriculture. Several criticisms have been addressed to their efficiency and effectiveness and thus, the design of innovative contract solutions is currently suggested. Among the novel contractual solutions, there are result-based payments, collective implementation of measures, the engagement of private and business actors in value chains, and new forms of land tenure systems coupled with environmental clauses. Little is known about the factors at interplay influencing farmers’ decision to uptake such contracts. The present paper investigates the acceptability determinants of the above-mentioned novel contractual solutions concerning a sample of nearly 1900 farmers from 10 European Union countries. The analysis is based on a questionnaire built through a common research framework. We apply ordered logistic regressions: both proportional and partial proportional odds models are used. Farmers’ preferences are interpreted by splitting the innovative contractual solutions into 13 individual contractual features which are then modeled in combination with the structural characteristics of the farms and the sociodemographic and behavioral characteristics of the farmers. We estimate farmers’ willingness to enroll in result-based, collective, value-chain, and land tenure contracts and highlight the positive and negative factors potentially influencing farmers’ acceptability of each type of contract
Preparation and microstructures of BaTi1-xZrxO3 hetero-epitaxial thin films on SrTiO3 substrates
International audienceZr-substituted BaTiO3 thin films (BaTi1−xZrxO3: BTZ) were prepared by rf-sputtering on SrTiO3 (STO) substrates or STO substrates buffered by pulsed laser deposited SrRuO3 (SRO) electrodes. The epitaxial growth of BTZ was monitored in situ by reflective high energy electron diffraction. X-ray reflectivity measurement demonstrated that a smooth surface with a roughness of 0.5 nm could be achieved under optimized deposition parameters. Film composition and the change in chemical environment due to Zr substitution are discussed based on electron energy-loss spectroscopy. The high quality of the interfaces was confirmed by transmission electron microscopy. The epitaxial relation between BTZ/SRO and SRO/STO was studied by selected area diffraction and high resolution transmission electron microscopy. All the results show that high quality epitaxial BTZ thin films were successfully deposited and ready for further electrical studies
A TEM analysis of the damage formation in thin GaN and AlN layers during rare earth ion implantation at medium range energy
Structural and optical characterization of Eu-implanted GaN
GaN was implanted with 300 keV Eu ions over a wide fluence range from 1 × 1013 to 1 × 1016 Eu cm−2 at room temperature (RT) or 500 °C. Detailed structural and optical characterizations of the samples were performed using Rutherford backscattering spectrometry and channelling, transmission and scanning electron microscopy, wavelength dispersive x-ray emission and RT cathodoluminescence (CL) spectroscopy. RT implantation results in a sigmoidal-shaped damage build-up curve with four regimes that were correlated with the formation of specific kinds of defects. After annealing at 1000 °C only samples implanted to fluences below 0.8 × 1015 Eu cm−2 showed near complete recovery of the crystal. Implantation at elevated temperature significantly decreases the implantation damage and increases the fraction of Eu incorporated on substitutional Ga-sites. The improved structural properties of samples implanted at elevated temperature are reflected in a higher intensity of Eu-related red light emission after annealing at 1000 °C. The RT CL intensity is correlated with the number of Eu ions on substitutional Ga-sites after annealing. Furthermore, a detailed study of optical activation shows that the optimum annealing temperature depends on the implantation fluence due to the sensitive balance of defects removed and created during high temperature annealing
Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: role of xenobiotic metabolising enzymes and intestinal microflora
International audienceWe investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of β-glucuronidase activity) following inulin consumption
Ultra-low energy ion implantation of Si into HfO2 and HfSiO-based structures for non volatile memory applications
International audienc
- …