Developments of Car Drive Train

Tato práce je kompilacního charakteru a uvádí prehled používaných prevodovek automobilu a jejich moderní vývojové trendy. Nejprve jsem se zabýval manuálními stupnovými prevodovkami, prevodovkami razenými pod zatížením a polosamocinnými prevodovkami. Ve druhé cásti jsem se podrobneji zabýval sekvencne razenými prevodovkami instalovanými do: sportovních vozu a bežných osobních automobilu.This work is compilatory character and features survey used gearboxes cars and their modern developments. First I deal with manual stepped gearboxes, powershift gearboxes and semi - automatic gearboxes. In second parts I, in more detail, deal with sequential shift mounted to the: sports cars, common motor – cars. KEY

Concept-driven visualization for terascale data analytics

Over the past couple of decades the amount of scientific data sets has exploded. The science community has since been facing the common problem of being drowned in data, and yet starved of information. Identification and extraction of meaningful features from large data sets has become one of the central problems of scientific research, for both simulation as well as sensory data sets. The problems at hand are multifold and need to be addressed concurrently to provide scientists with the necessary tools, methods, and systems. Firstly, the underlying data structures and management need to be optimized for the kind of data most commonly used in scientific research, i.e. terascale time-varying, multi-dimensional, multi-variate, and potentially non-uniform grids. This implies avoidance of data duplication, utilization of a transparent query structure, and use of sophisticated underlying data structures and algorithms.Secondly, in the case of scientific data sets, simplistic queries are not a sufficient method to describe subsets or features. For time-varying data sets, many features can generally be described as local events, i.e. spatially and temporally limited regions with characteristic properties in value space. While most often scientists know quite well what they are looking for in a data set, at times they cannot formally or definitively describe their concept well to computer science experts, especially when based on partially substantiated knowledge. Scientists need to be enabled to query and extract such features or events directly and without having to rewrite their hypothesis into an inadequately simple query language. Thirdly, tools to analyze the quality and sensitivity of these event queries itself are required. Understanding local data sensitivity is a necessity for enabling scientists to refine query parameters as needed to produce more meaningful findings.Query sensitivity analysis can also be utilized to establish trends for event-driven queries, i.e. how does the query sensitivity differ between locations and over a series of data sets. In this dissertation, we present an approach to apply these interdependent measures to aid scientists in better understanding their data sets. An integrated system containing all of the above tools and system parts is presented

Dual-use research and the H5N1 bird flu: Is restricting publication the solution to biosecurity issues?

Recent studies altering the host range of the H5N1 bird flu virus have refueled intense debates over the potential misuse of academic life science research. To curtail the bioterrorism threat, it has been suggested that dissemination of the research results and methodology should be restricted. However, doubts have been raised over the suitability and effectiveness of this measure. Using the H5N1 studies as an example, this paper summarizes the main arguments of the debate. Particular attention is paid to the issue of the tacit knowledge required to replicate published life science research results, which has so far received limited attention. Taking into account the importance of tacit knowledge for life science research, it is argued that preventing publication of the methodology does not decrease the threat of bioterroris

Small Angle Scattering data analysis for dense polydisperse systems: the FLAC program

FLAC is a program to calculate the small-angle neutron scattering intensity of highly packed polydisperse systems of neutral or charged hard spheres within the Percus-Yevick and the Mean Spherical Approximation closures, respectively. The polydisperse system is defined by a size distribution function and the macro-particles have hard sphere radii which may differ from the size of their scattering cores. With FLAC, one can either simulate scattering intensities or fit experimental small angle neutron scattering data. In output scattering intensities, structure factors and pair correlation functions are provided. Smearing effects due to instrumental resolution, vertical slit, primary beam width and multiple scattering effects are also included on the basis of the existing theories. Possible form factors are those of filled or two-shell spheres.Comment: 18 pages, 1 figure, uses elsart.st

Inverse ISAsomes in Bio-Compatible Oils—Exploring Formulations in Squalane, Triolein and Olive Oil

In contrast to their more common counterparts in aqueous solutions, inverse ISAsomes (internally self-assembled somes/particles) are formulated as kinetically stabilised dispersions of hydrophilic, lyotropic liquid-crystalline (LC) phases in non-polar oils. This contribution reports on their formation in bio-compatible oils. We found that it is possible to create inverse hexosomes, inverse micellar cubosomes (Fd3m) and an inverse emulsified microemulsion (EME) in excess squalane with a polyethylene glycol alkyl ether as the primary surfactant forming the LC phase and to stabilise them with hydrophobised silica nanoparticles. Furthermore, an emulsified L1-phase and inverse hexosomes were formed in excess triolein with the triblock-copolymer Pluronic® P94 as the primary surfactant. Stabilisation was achieved with a molecular stabiliser of type polyethylene glycol (PEG)-dipolyhydroxystearate. For the inverse hexosomes in triolein, the possibility of a formulation without any additional stabiliser was explored. It was found that a sufficiently strong stabilisation effect was created by the primary surfactant alone. Finally, triolein was replaced with olive oil which also led to the successful formation of inverse hexosomes. As far as we know, there exists no previous contribution about inverse ISAsomes in complex oils such as triolein or plant oils, and the existence of stabiliser-free (i.e., self-stabilising) inverse hexosomes has also not been reported until now

Biomaterials Redesign of Microvascular Anastomotic Coupler

The key factors in the redesign of this part include the use of biomaterials, the redesign of the part geometry to include these biomaterials, and the proposition of production scale manufacturing methods suitable for these redesigns. The choice of biodegradable material was guided by previous literature, during which we found the PLGA polymer used in similar predicate devices with a biodegradability timeline similar to our goals. The model of coupler serving as the inspiration for the redesign utilizes two very strong but bioinert materials, polyethylene, and stainless steel. These high strength materials can withstand much greater forces than the biodegradable bulk material chosen for the redesign, so features of the old design were modified for robustness. This included to realize this new design on a production scale, the two most promising possibilities include injection molding with the PLGA polymer or additive manufacturing using stereo lithography printers and biodegradable resins. The commonality of these two processes is the ability to make complex shapes while retaining isotropic materials in the final part, which is essential for the strength of small features such as the pins. Key customer requirements for anastomotic procedures include successful attachments of vessels and low rates of dehiscence. These requirements are related to our coupler by successful attachment rate, max force withstood by coupler before assembly separates, max forces withstood by vessels before ripping. Different models of the coupler device were tested in assembled orientation with one blood vessel surrogate engaged. In both models, tensions upwards of 100N were experienced without a signal failure of the pins. This demonstrates that the pins are exceptionally stronger when assembled into the opposite coupler than when unengaged. Another factor in the improvement of the device is the time taken to film an implementation of the device using dissected cow arteries. The first round of prototypes required several people to affix the coupler and blood vessels and required additional processing of the cow arteries in the form of puncturing pilot holes for the spikes. Overall, the procedure took about an hour to perform. The latest model of prototypes was operated on in only a half hour and required only one person to perform the procedure. The improved placement and orientation of the spikes allow for much easier puncturing of the arterial wall for smoother attachments

An integrated workflow for charting the human interaction proteome: insights into the PP2A system

Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP-MS studies on human protein complexes