Fatty oil accumulation in vegetable soybean seeds and its thin-layer chromatography

Received: February 23rd, 2021 ; Accepted: May 5th, 2021 ; Published: May 20th, 2021 ; Correspondence: [email protected] paper studies the accumulation of crude oil (triacylglycerides, monoacylglycerides, diacylglycerides, free fatty acids, phospholipids, tocopherols, pigments, sterols, waxes) in soybean vegetable samples. Samples were taken from two groups: grown in an experimental field and in protected ground of the Federal Scientific Center for Vegetable Growing in the Moscow Region. Both groups were observed in the phase of technical ripeness and in the phase of complete biological ripeness (finally ripe seeds). Soxhlet method as arbitration in analysis was used as suitable for the extraction of lipophilic substances. It was determined that the fat content in the technical ripeness phase in most soybean samples averaged 10.5%. In the phase of biological ripeness, the highest accumulation of fatty oil was observed in Hidaka and Nordic (17.6%). The oil content in vegetable forms of soybeans was consistently lower than that of grain varieties: in the phases of technical and biological ripeness by 55.6% and 22.0% (in relative values) respectively. Thus, he accumulation of oil in seeds is determined mainly genetically. The refractive index of vegetable and oil soybean was established equal on average 1.4755. According to this finding the soybean oil can be classified as semi-drying. Thin layer chromatography (TLC) was used to study the lipophilic components of soybean fatty oil. It was found experimentally that the best separation of the components is achieved using an eluent system: carbon tetrachloride: chloroform in a 2: 3 ratio. It was found that the main fatsoluble compounds are the following (in order of increasing Rf in the chromatogram): phospholipids, monoacylglycerides, triacylglycerides, tocopherols, fatty acid esters. As a finding of the research vegetable soybean cultivated at 55 °N in both technical and biological ripeness phases significantly accumulate crude oil in the seeds. This crude oil contained ω-6, ω-3, phospholipids, and vitamin E

A new beamline for laser spin-polarization at ISOLDE

A beamline dedicated to the production of laser-polarized radioactive beams has been constructed at ISOLDE, CERN. We present here different simulations leading to the design and construction of the setup, as well as technical details of the full setup and examples of the achieved polarizations for several radioisotopes. Beamline simulations show a good transmission through the entire line, in agreement with observations. Simulations of the induced nuclear spin-polarization as a function of atom-laser interaction length are presented for $^{26,28}$Na, [1] and for $^{35}$Ar, which is studied in this work. Adiabatic spin rotation of the spin-polarized ensemble of atoms, and how this influences the observed nuclear ensemble polarization, are also performed for the same nuclei. For $^{35}$Ar, we show that multiple-frequency pumping enhances the ensemble polarization by a factor 1.85, in agreement with predictions from a rate equations model. [1] J. Phys. G: Nucl. Part. Phys./174408400

Isomer shift and magnetic moment of the long-lived 1/2+^{+} isomer in 3079^{79}_{30}Zn49_{49}: signature of shape coexistence near 78^{78}Ni

Collinear laser spectroscopy has been performed on the $^{79}_{30}$Zn$_{49}$ isotope at ISOLDE-CERN. The existence of a long-lived isomer with a few hundred milliseconds half-life was confirmed, and the nuclear spins and moments of the ground and isomeric states in $^{79}$Zn as well as the isomer shift were measured. From the observed hyperfine structures, spins $I = 9/2$ and $I = 1/2$ are firmly assigned to the ground and isomeric states. The magnetic moment $\mu$ ($^{79}$Zn) = $-$1.1866(10) $\mu_{\rm{N}}$, confirms the spin-parity $9/2^{+}$ with a $\nu g_{9/2}^{-1}$ shell-model configuration, in excellent agreement with the prediction from large scale shell-model theories. The magnetic moment $\mu$ ($^{79m}$Zn) = $-$1.0180(12) $\mu_{\rm{N}}$ supports a positive parity for the isomer, with a wave function dominated by a 2h-1p neutron excitation across the $N = 50$ shell gap. The large isomer shift reveals an increase of the intruder isomer mean square charge radius with respect to that of the ground state: $\delta \langle r^{2}_{c}\rangle^{79,79m}$ = +0.204(6) fm$^{2}$, providing first evidence of shape coexistence.Comment: 5 pages, 4 figures, 1 table, Accepeted by Phys. Rev. Lett. (2016

ПЕРСПЕКТИВНОСТЬ ИНТРОДУКЦИИ НЕТРАДИЦИОННЫХ ПЛОДОВЫХ, ЯГОДНЫХ И ОВОЩНЫХ КУЛЬТУР В УСЛОВИЯХ ДАГЕСТАНА (XI МЕЖДУНАРОДНАЯ НАУЧНО-МЕТОДИЧЕСКАЯ КОНФЕРЕНЦИЯ «ИНТРОДУКЦИЯ, СОХРАНЕНИЕ И ИСПОЛЬЗОВАНИЕ БИОЛОГИЧЕСКОГО РАЗНООБРАЗИЯ КУЛЬТУРНЫХ РАСТЕНИЙ» 10-14 ИЮНЯ 2014

June 9-13, 2014 in Makhachkala hosted XI International scientific-methodical conference on the theme: «Introduction, conservation and use of biological diversity of cultivated plants», organized by FGBNU VNIISSOK, Dagestan Research Institute for Agriculture and GBS DSC RAS. The conference was attended by scientists from Russia, CIS and foreign countries. Due to the conference Dagestan turned out to be a prime location for the cultivation of both traditional and non-traditional plants with a high content of biologically active substances, as well as a training ground for resistance tests because of the combination of mountain and plain zones.9-13 июня 2014 года в Махачкале прошла XI Международная научно-методическая конференция на тему: «Интродукция, сохранение и использование биологического разнообразия культурных растений», организованная ФГБНУ ВНИИССОК, Дагестанским НИИСХ и ГБС ДНЦ РАН. В конференции приняли участие ученые России, ближнего и дальнего зарубежья. По итогам конференции принято заключение, что Дагестан является благоприятным регионом для возделывания как традиционных, так и нетрадиционных растений с повышенным содержанием БАВ, а так же полигоном для испытаний на устойчивость, благодаря сочетанию двух зон: горной и равнинной

Analysis of risk factors for COVID-19-related fatal outcome in 337991 patients with type 1 and type 2 diabetes mellitus in 2020–2022 years: Russian nationwide retrospective study

BACKGROUND: The coronavirus pandemic has had an extremely negative impact on the patients with diabetes mellitus (DM both in terms of a more severe course of COVID -19 and an increased risk of death.AIM: Analysis of risk factors for death due to COVID -19 in patients with DM type 1 and type 2 (DM1 and DM2).MATERIALS AND METHODS: Retrospective analysis of the database of the national diabetes register (NDR), which included DM patients with COVID-19 and reported virus infection outcome (recovery/or death) in 15 712 DM1 and 322 279 DM2 patients during a 2-year follow-up period (01/02/2020 to 03/04/2022) (discharge date)).RESULTS: Case fatality rate in patients with DM, who underwent COVID -19 was 17.1% (DM1–8.8%; DM2–17.5%). As a result of multivariate regression analysis of seven significant factors in DM1 and thirteen in DM2 (evaluated by univariate anlisys), a number of the most important predictors of risk for fatal outcome were identified: in DM1 these were age ≥65 years (OR =4.01, 95% CI: 1.42–11.36), presence of arterial hypertension (AH) (OR =2.72, 95% CI: 1.03 -7.16) and diabetic foot syndrome (DFS) (OR = 7.22, 95% CI: 1.98–26.29); for T2DM: age ≥ 65 years (OR =2.53, 95% CI: 1.96–3.27), male (OR =1.51, 95% CI: 1.23–1.84), duration DM ≥10 years (OR =2.01, 95% CI: 1.61–2.51), BMI ≥ 30 kg/m2 (OR =1.26, 95% CI: 1.02–1.55), ASCVD/CKD (OR =1.49, 95% CI: 1.01–2.04), history of diabetic coma (OR =12.97, 95% CI: 1.89–88.99) and presence of disability ( OR =1.40, 95% CI: 1.14–1.73). In T2DM, the type of antidiabetic therapy (ADT) prior to COVID -19 (last visit before the development of infection) had a significant impact: Insulin therapy (OR = 1.64, 95% CI: 1.30–2.07), sulfonylureas (SU) (OR =1.51, 95% CI: 1.23–1.84)); dipeptidyl peptidase-4 inhibitor (iDPP-4) therapy (OR =0.57, 95% CI: 0.39–0.83) and sodium-glucose cotransporter-2 inhibitor (iSGLT2) therapy (OR =0.64, 95% CI: 0.46–0.88). Vaccination was the most important protective factor in both types of DM: DM1 OR =0.19, 95% CI: 0.06–0.59; SD2 OR =0.20, 95% CI: 0.16–0.26.CONCLUSION: The common risk factor for fatal outcome in both DM1 and DM2 was age ≥65 years; in DM1 — history of hypertension and DFS, in DM2 — male sex, diabetes duration ≥10 years, BMI ≥30 kg/m2, history of ASCVD/CKD and diabetic coma, disability. In T2DM, significant differences in risk were observed depending on the type of ADT: insulin and SU therapy were factors that increased the risk of death, whereas therapy with iDPP-4 and iSGLT2 reduced the risk of death. Vaccination reduced the risk of death in DM1 and DM2 by 5.2 and 5-fold, respectively

Electromagnetic Properties of Indium Isotopes Elucidate the Doubly Magic Character of ¹⁰⁰Sn

Understanding the nuclear properties in the vicinity of 100Sn – suggested to be the heaviest doubly magic nucleus with equal proton number Z and neutron number N – has been a long-standing challenge for experimental and theoretical nuclear physics. In particular, contradictory experimental evidence exists regarding the role of nuclear collectivity in this region of the nuclear chart. Here, we provide additional evidence for the doubly-magic character of 100Sn by measuring the ground-state electromagnetic moments and nuclear charge radii of indium (Z = 49) isotopes as N approaches 50 from above using precision laser spectroscopy. Our results span almost the complete range between the two major neutron closed shells at N = 50 and N = 82 and reveal parabolic trends as a function of the neutron number, with a clear reduction toward these two neutron closed-shells. A detailed comparison between our experimental and numerical results from two complementary nuclear many-body frameworks, density functional theory and ab initio methods, exposes deficiencies in nuclear models and establishes a benchmark for future theoretical developments.<br/

Evolution of nuclear structure in neutron-rich odd-Zn isotopes and isomers

Collinear laser spectroscopy was performed on Zn (Z=30) isotopes at ISOLDE, CERN. The study of hyperfine spectra of nuclei across the Zn isotopic chain, N=33–49, allowed the measurement of nuclear spins for the ground and isomeric states in odd-A neutron-rich nuclei up to N=50. Exactly one long-lived (>10 ms) isomeric state has been established in each 69–79Zn isotope. The nuclear magnetic dipole moments and spectroscopic quadrupole moments are well reproduced by large-scale shell–model calculations in the f5pg9 and fpg9d5 model spaces, thus establishing the dominant term in their wave function. The magnetic moment of the intruder Iπ=1/2+ isomer in 79Zn is reproduced only if the νs1/2 orbital is added to the valence space, as realized in the recently developed PFSDG-U interaction. The spin and moments of the low-lying isomeric state in 73Zn suggest a strong onset of deformation at N=43, while the progression towards 79Zn points to the stability of the Z=28 and N=50 shell gaps, supporting the magicity of 78Ni

ВЛИЯНИЕ СУММАРНОГО СОДЕРЖАНИЯ ВОДОРАСТВОРИМЫХ АНТИОКСИДАНТОВ В КОРНЕВИЩАХ НА ЗИМОСТОЙКОСТЬ СОРТОВ ИРИСА САДОВОГО (IRIS HYBRIDA L.)

The total content of watersoluble antioxidants in the rhizomes of garden iris varies depending on the hardiness of the variety. Domestic varieties have better winter hardiness than varieties of foreign breeding. The amount of antioxidant content in the rhizomes at the winter-hardy varieties of iris garden after wintering is significantly reduced. The direct positive correlation between of the average degree of the total content of antioxidants in the rhizomes of iris garden and winter hardiness was revealed. The correlation coefficient is r=0,66. Therefore, the total content of water-soluble antioxidants in the rhizomes of iris garden can be apply as a potential marker of long hardiness source material.Суммарное содержание водорастворимых антиоксидантов в корневищах ириса садового варьирует в зависимости от зимостойкости сорта. Отечественные сорта обладают более высокой зимостойкостью, чем сорта зарубежной селекции, причём, содержание суммы антиоксидантов в корневищах у зимостойких сортов ириса садового после перезимовки существенно снижается, а у незимостойких сортов – увеличивается. Нами установлена прямая положительная корреляция средней степени между величиной суммарного содержания антиоксидантов в корневищах ириса садового и зимостойкостью. Коэффициент корреляции r=0,66. Следовательно, суммарное содержание водорастворимых антиоксидантов в корневищах ириса садового может служить потенциальным маркером зимостойкости исходного сортового материала

Magnetic moments of short-lived nuclei with part-per-million accuracy: Towards novel applications of β\beta-detected NMR in physics, chemistry and biology

We determine for the first time the magnetic dipole moment of a short-lived nucleus with part-per-million (ppm) accuracy. To achieve this two orders of magnitude improvement over previous studies, we implement a number of innovations into our $\beta$-detected Nuclear Magnetic Resonance ($\beta$-NMR) setup at ISOLDE/CERN. Using liquid samples as hosts we obtain narrow, sub-kHz linewidth, resonances, while a simultaneous in-situ $^1$H NMR measurement allows us to calibrate and stabilize the magnetic field to ppm precision, thus eliminating the need for additional $\beta$-NMR reference measurements. Furthermore, we use ab initio calculations of NMR shielding constants to improve the accuracy of the reference magnetic moment, thus removing a large systematic error. We demonstrate the potential of this combined approach with the 1.1 s half-life radioactive nucleus $^{26}$Na, which is relevant for biochemical studies. Our technique can be readily extended to other isotopic chains, providing accurate magnetic moments for many short-lived nuclei. Furthermore, we discuss how our approach can open the path towards a wide range of applications of the ultra-sensitive $\beta$-NMR in physics, chemistry, and biology.Comment: re-submitte