Polymer sieving matrices in microanalytical electrophoresis

Microfluidic design has advanced existing protein separation capabilities and supported novel assays. Key metrics for successful protein separations include fast, robust, and sensitive analysis of complex mixtures of bio-macromolecules. Attaining high separation resolution is a chief concern. Here we review recent advances in polymer-based electrophoresis sieving materials that are impacting microfluidic bioanalytical applications. Looking forward, we comment on unmet needs for advanced separation media in micro-to-nanoscale devices

Activism for Sex Workers in the Netherlands: Interview with Jan Visser about Foundation and End of De Rode Draad, 1976 to 2012

By order of the Archive and Centre for the Documentation of Sex Work — Madonna (Bochum), I conduct interviews with sex workers and sex work activists as contemporary witnesses. In this context, I met the sociologist Jan Visser in January 2018 for a first conversation about his political work in connection with the de-criminalisation and de-stigmatisation of sex workers and sex work.For the Journal of Social History and the History of Social Movements, an abbreviated excerpt of this conversation was written to trace a chronical arch from the development to the dissolution of the prostitutes’ rights organisation De Rode Draad. In 1985, a group of female sex workers formed De Rode Draad in Amsterdam, which was subsidised by the Dutch government from the get-go. De Rode Draad was involved in the development of a concept of decriminalisation of prostitution by regulation and integration of the sexwork into labour and employment laws. Their history, work and success is closely related to the Mr. A. de Graaf Foundation.Furthermore, the interview highlights the specific frictions and challenges, which a group is forced to deal with, when stigmatised sex work activists and academic allies who are no sex workers cooperate

POLYDOM, UNE NOUVELLE PROTEINE COMPOSEE D'UNE ASSOCIATION UNIQUE DE DOMAINES EXTRACELLULAIRES

LE RENOUVELLEMENT PERMANENT DES CELLULES SANGUINES CIRCULANTES EST ASSURE PAR UN PETIT NOMBRE DE CELLULES SOUCHES HEMATOPOIETIQUES (CSH), LOCALISEES DANS LA MOELLE OSSEUSE CHEZ L'ADULTE. LE MICROENVIRONNEMENT MEDULLAIRE SYNTHETISE LA MAJORITE DES FACTEURS QUI REGULENT LES DIFFERENTES ETAPES DU DEVELOPPEMENT DES CSH EN CELLULES HEMATOPOIETIQUES MATURES ET FONCTIONNELLES. ACTUELLEMENT, DE NOMBREUX EFFECTEURS QUI PARTICIPENT AU DIALOGUE MOLECULAIRE ENTRE LES CELLULES STROMALES DU MICROENVIRONNEMENT MEDULLAIRE ET LES CSH, NE SONT PAS CONNUS. MON TRAVAIL DE THESE S'EST ORIENTE SUR L'IDENTIFICATION DE NOUVELLES MOLECULES PRODUITES PAR LA LIGNEE MS-5 QUI MIME IN VITRO LE STROMA MEDULLAIRE. NOUS AVONS UTILISE UNE APPROCHE DE RT-PCR ( REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ) A PARTIR D'OLIGONUCLEOTIDES DEGENERES PERMETTANT D'IDENTIFIER DES GENES CONTENANT DES DOMAINES EGF-LIKE SEMBLABLES A CEUX DES GENES NOTCH. NOUS AVONS AINSI ISOLE UN NOUVEL ADN COMPLEMENTAIRE QUE NOUS AVONS ENTIEREMENT CLONE ET SEQUENCE. CET ADNC CODE POUR UNE PROTEINE EXTRACELLULAIRE DE 387 KILODALTON, QUE NOUS AVONS APPELEE POLYDOM. CETTE PROTEINE CONTIENT UN PEPTIDE SIGNAL SUIVI D'UN ENCHAINEMENT ORIGINAL DE HUIT MODULES DIFFERENTS QUI INCLUENT UN DOMAINE PENTRAXINE, UN DOMAINE VWF-A, DIX REPETITIONS EGF-LIKE, 34 MODULES CCP ET DEUX MODULES HYALINE REPEAT . L'ANALYSE DE CE DERNIER MODULE, NOUS A AMENE A DECRIRE UNE NOUVELLE FAMILLE DE MODULES PROTEIQUES EXTRACELLULAIRES QUI APPARTIENT A LA SUPERFAMILLE DE REPLIEMENT DE TYPE IMMUNOGLOBULINE. PARALLELEMENT A L'ETUDE STRUCTURALE DE POLYDOM, NOUS AVONS ANALYSE LE PROFIL D'EXPRESSION DU GENE CORRESPONDANT, NOTAMMENT AU SEIN DU TISSU HEMATOPOIETIQUE. L'ENSEMBLE DE NOS RESULTATS SUGGERENT QUE POLYDOM POURRAIT AVOIR UN ROLE BIOLOGIQUE IMPORTANT DANS L'ADHESION DES CELLULES STROMALES MEDULLAIRES OU/ET DANS LES REACTIONS IMMUNITAIRES INNEES.PARIS-BIUSJ-Thèses (751052125) / SudocCentre Technique Livre Ens. Sup. (774682301) / SudocPARIS-BIUSJ-Physique recherche (751052113) / SudocSudocFranceF

Open space in Hong Kong: assessing the sustainability of development

published_or_final_versionEnvironmental ManagementMasterMaster of Science in Environmental Managemen