EXPLORING ACTIVE EUROPEAN CITIZENSHIP: AN EXPERIMENTAL PROJECT OF PROMOTION AND COMMUNICATION

The European Union celebrates its thirty years anniversary in a peculiar historical moment characterized by crises and emergencies. Being able to count on an informed and active citizenship becomes increasingly necessary. Universities, schools and institutions are teaming up in order to propose projects for sharing information about and promoting the European Union involving all its citizens. Young people, in particular, are identified among the first interlocutors, to whom we entrust communication between peers, exploiting the potential of digital media. In a context often dominated by fake-news, complete and source-aware information about the European Union becomes an essential tool for citizens' empowerment. This article proposes a reflection starting from a multi-sited research conducted in the Italian cities of Bologna, Forlì and Ravenna through a project of the University of Bologna and the Emilia-Romagna Region entitled "Promoting active European citizenship in times of crisis". Through participant observation, the article reflects on the results emerged from word-cafés, workshops and meetings with citizens developed in three different territorial areas and with the involvement of three different targets: first and second grade high school students, university students and adult citizens. Article visualizations

Prevalence and characteristics of diffuse idiopathic skeletal hyperostosis (DISH) in Italy

Purpose Diffuse idiopathic skeletal hyperostosis (DISH) is a benign condition characterized by ossification of the spine and prominent enthesopathies. Highly heterogeneous epidemiological figures have been reported in the literature, while in Italy the largest study has been conducted in 1992. The aim of our research is to contribute updated information about prevalence of DISH in Italy and to describe the clinical and radiographic characteristics associated with the disorder. Material and methods A retrospective review of lumbosacral spine, thoracic spine and pelvis radiographs was performed. Consecutive patients visiting the emergency department of our Institution over 3 years were enrolled. Presence of DISH was evaluated applying the Resnick and Niwayama criteria. Clinical and radiological features were also assessed. Results We included 1012 individuals (60.6% women), and DISH was present in 130 cases. The overall prevalence of DISH was 12.8% (95% CI 10.8-15.1), with higher figures in the male sample (16.8%) than in females (10.3%). In binary logistic regression adjusted for age, BMI (OR 1.50, p < 0.001) diabetes (OR 1.85, p = 0.003), hypertension (OR 2.04, p = 0.007) ischiopubic enthesopathy (OR 7.08, p < 0.001), iliac crest enthesopathy (OR 4.63, p < 0.001) and greater trochanter enthesopathy (OR 3.51, p < 0.001), were significantly associated with the condition. Conclusion The prevalence of DISH observed in our study is consistent with previous literature, and we confirm that the disorder is more frequently retrieved in men and that it is associated with the presence of metabolic disorders and pelvic enthesopathy. Knowledge about the epidemiology and characteristics of DISH is needed to properly identify the condition

A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers

BACKGROUND: Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin. METHODS: Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p<0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. To validate the expression changes found with 2DE of 5 proteins (14-3-3 protein zeta/delta, cyclophilin A, Cystatin-C, Protein S100-A7, and zinc-alpha-2-glycoprotein), we used the western blot analysis. Moreover, proteins differentially expressed were functionally analyzed using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved. RESULTS: The analysis of the protein profiles allowed us to find 13 proteins with a different expression in CFS in respect to control. Nine spots were up-regulated in CFS and 4 down-regulated. These proteins belong to different functional classes, such as inflammatory response, immune system and metabolism. In particular, as shown by the pathway analysis, the network built with our proteins highlights the involvement of inflammatory response in CFS pathogenesis. CONCLUSIONS: This study shows the presence of differentially expressed proteins in the saliva of the couple of monozygotic twins discordant for CFS, probably related to the disease. Consequently, we believe the proteomic approach could be useful both to define a panel of potential diagnostic biomarkers and to shed new light on the comprehension of the pathogenetic pathways of CFS

Comisión de Promoción y Desarrollo de la Investigación Acta 8

1- Revisión de las fortalezas, oportunidades y debilidades identificadas sobre pertinencia institucional local/global. 2- Trabajo en comisión para listar las amenazas / problemas / riesgos. 3- Definir objetivos y posibles líneas de acción.Fil: Ames, Cecilia. Universidad Nacional de Córdoba. Secretaría de Ciencia y Tecnología; Argentina.Fil: Ateca, María Rosa. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.Fil: Fernández, Alicia Ruth. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina.Fil: Giacomelli, Carla. Universidad Nacional de Córdoba. Secretaría de Ciencia y Tecnología; Argentina.Fil: Guido, Mario Eduardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Herz, Marcelo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Monti, Gustavo Alberto. Facultad de Matemática, Astronomía, Física y Computación. Universidad Nacional de Córdoba; Argentina.Fil: Recalde, María Luisa. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades; Argentina.Fil: Recalde, María Luisa. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina.Fil: Rueda, Nelly Elena María. Universidad Nacional de Córdoba. Facultad de Lenguas; Argentina.Fil: Strumia, Miriam. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina

Comisión de Promoción y Desarrollo de la Investigación Informe 8

Octava dimensión de análisis transversal: Desarrollo, aplicación efectiva de los recursos humanos, organizacionales, técnicos, materiales y financieros.Fil: Ames, Cecilia. Universidad Nacional de Córdoba. Secretaría de Ciencia y Tecnología; Argentina.Fil: Ateca, María Rosa. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.Fil: Fernández, Alicia Ruth. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina.Fil: Giacomelli, Carla. Universidad Nacional de Córdoba. Secretaría de Ciencia y Tecnología; Argentina.Fil: Guido, Mario Eduardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Herz, Marcelo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Monti, Gustavo Alberto. Facultad de Matemática, Astronomía, Física y Computación. Universidad Nacional de Córdoba; Argentina.Fil: Recalde, María Luisa. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades; Argentina.Fil: Recalde, María Luisa. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina.Fil: Rueda, Nelly Elena María. Universidad Nacional de Córdoba. Facultad de Lenguas; Argentina.Fil: Strumia, Miriam. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina

Addressing the clinical unmet needs in primary Sjögren's Syndrome through the sharing, harmonization and federated analysis of 21 European cohorts

For many decades, the clinical unmet needs of primary Sjögren's Syndrome (pSS) have been left unresolved due to the rareness of the disease and the complexity of the underlying pathogenic mechanisms, including the pSS-associated lymphomagenesis process. Here, we present the HarmonicSS cloud-computing exemplar which offers beyond the state-of-the-art data analytics services to address the pSS clinical unmet needs, including the development of lymphoma classification models and the identification of biomarkers for lymphomagenesis. The users of the platform have been able to successfully interlink, curate, and harmonize 21 regional, national, and international European cohorts of 7,551 pSS patients with respect to the ethical and legal issues for data sharing. Federated AI algorithms were trained across the harmonized databases, with reduced execution time complexity, yielding robust lymphoma classification models with 85% accuracy, 81.25% sensitivity, 85.4% specificity along with 5 biomarkers for lymphoma development. To our knowledge, this is the first GDPR compliant platform that provides federated AI services to address the pSS clinical unmet needs. © 2022 The Author(s

Mortality risk factors in primary Sjögren syndrome:a real-world, retrospective, cohort study

BACKGROUND: What baseline predictors would be involved in mortality in people with primary Sjögren syndrome (SjS) remains uncertain. This study aimed to investigate the baseline characteristics collected at the time of diagnosis of SjS associated with mortality and to identify mortality risk factors for all-cause death and deaths related to systemic SjS activity measured by the ESSDAI score.METHODS: In this international, real-world, retrospective, cohort study, we retrospectively collected data from 27 countries on mortality and causes of death from the Big Data Sjögren Registry. Inclusion criteria consisted of fulfilling 2002/2016 SjS classification criteria, and exclusion criteria included chronic HCV/HIV infections and associated systemic autoimmune diseases. A statistical approach based on a directed acyclic graph was used, with all-cause and Sjögren-related mortality as primary endpoints. The key determinants that defined the disease phenotype at diagnosis (glandular, systemic, and immunological) were analysed as independent variables.FINDINGS: Between January 1st, 2014 and December 31, 2023, data from 11,372 patients with primary SjS (93.5% women, 78.4% classified as White, mean age at diagnosis of 51.1 years) included in the Registry were analysed. 876 (7.7%) deaths were recorded after a mean follow-up of 8.6 years (SD 7.12). Univariate analysis of prognostic factors for all-cause death identified eight Sjögren-related variables (ocular and oral tests, salivary biopsy, ESSDAI, ANA, anti-Ro, anti-La, and cryoglobulins). The multivariate CPH model adjusted for these variables and the epidemiological features showed that DAS-ESSDAI (high vs no high: HR = 1.68; 95% CI, 1.27-2.22) and cryoglobulins (positive vs negative: HR = 1.72; 95% CI, 1.22-2.42) were independent predictors of all-cause death. Of the 640 deaths with available information detailing the specific cause of death, 14% were due to systemic SjS. Univariate analysis of prognostic factors for Sjögren-cause death identified five Sjögren-related variables (oral tests, clinESSDAI, DAS-ESSDAI, ANA, and cryoglobulins). The multivariate competing risks CPH model adjusted for these variables and the epidemiological features showed that oral tests (abnormal vs normal results: HR = 1.38; 95% CI, 1.01-1.87), DAS-ESSDAI (high vs no high: HR = 1.55; 95% CI, 1.22-1.96) and cryoglobulins (positive vs negative: HR = 1.52; 95% CI, 1.16-2) were independent predictors of SjS-related death.INTERPRETATION: The key mortality risk factors at the time of SjS diagnosis were positive cryoglobulins and a high systemic activity scored using the ESSDAI, conferring a 2-times increased risk of all-cause and SjS-related death. ESSDAI measurement and cryoglobulin testing should be considered mandatory when an individual is diagnosed with SjS.FUNDING: Novartis.</p