Catastrophic Decline of World's Largest Primate: 80% Loss of Grauer's Gorilla (Gorilla beringei graueri) Population Justifies Critically Endangered Status

Grauer's gorilla (Gorilla beringei graueri), the World's largest primate, is confined to eastern Democratic Republic of Congo (DRC) and is threatened by civil war and insecurity. During the war, armed groups in mining camps relied on hunting bushmeat, including gorillas. Insecurity and the presence of several militia groups across Grauer's gorilla's range made it very difficult to assess their population size. Here we use a novel method that enables rigorous assessment of local community and ranger-collected data on gorilla occupancy to evaluate the impacts of civil war on Grauer's gorilla, which prior to the war was estimated to number 16,900 individuals. We show that gorilla numbers in their stronghold of Kahuzi- Biega National Park have declined by 87%. Encounter rate data of gorilla nests at 10 sites across its range indicate declines of 82-100% at six of these sites. Spatial occupancy analysis identifies three key areas as the most critical sites for the remaining populations of this ape and that the range of this taxon is around 19,700 km2. We estimate that only 3,800 Grauer's gorillas remain in the wild, a 77% decline in one generation, justifying its elevation to Critically Endangered status on the IUCN Red List of Threatened Species

An orally available, small-molecule interferon inhibits viral replication

Most acute hepatitis C virus (HCV) infections become chronic and some progress to liver cirrhosis or hepatocellular carcinoma. Standard therapy involves an interferon (IFN)-α-based regimen, and efficacy of therapy has been significantly improved by the development of protease inhibitors. However, several issues remain concerning the injectable form and the side effects of IFN. Here, we report an orally available, small-molecule type I IFN receptor agonist that directly transduces the IFN signal cascade and stimulates antiviral gene expression. Like type I IFN, the small-molecule compound induces IFN-stimulated gene (ISG) expression for antiviral activity in vitro and in vivo in mice, and the ISG induction mechanism is attributed to a direct interaction between the compound and IFN-α receptor 2, a key molecule of IFN-signaling on the cell surface. Our study highlights the importance of an orally active IFN-like agent, both as a therapy for antiviral infections and as a potential IFN substitute

STAT2 Mediates Innate Immunity to Dengue Virus in the Absence of STAT1 via the Type I Interferon Receptor

Dengue virus (DENV) is a mosquito-borne flavivirus, and symptoms of infection range from asymptomatic to the severe dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). High viral loads correlate with disease severity, and both type I & II interferons (IFNs) are crucial for controlling viral replication. We have previously reported that signal transducer and activator of transcription (STAT) 1-deficient mice are resistant to DENV-induced disease, but little is known about this STAT1-independent mechanism of protection. To determine the molecular basis of the STAT1-independent pathway, mice lacking STAT1, STAT2, or both STAT1 and STAT2 were infected with a virulent mouse-adapted strain of DENV2. In the first 72 hours of infection, the single-deficient mice lacking STAT1 or STAT2 possessed 50–100 fold higher levels of viral RNA than wild type mice in the serum, spleen, and other visceral tissues, but remained resistant to DENV-induced death. In contrast, the double-deficient mice exhibited the early death phenotype previously observed in type I and II IFN receptor knockout mice (AG129), indicating that STAT2 is the mediator of the STAT1-independent host defense mechanism. Further studies demonstrated that this STAT2-dependent STAT1-independent mechanism requires the type I IFN receptor, and contributes to the autocrine amplification of type I IFN expression. Examination of gene expression in the spleen and bone marrow-derived macrophages following DENV infection revealed STAT2-dependent pathways can induce the transcription of a subset of interferon stimulated genes even in the absence of STAT1. Collectively, these results help elucidate the nature of the poorly understood STAT1-independent host defense mechanism against viruses by identifying a functional type I IFN/STAT2 signaling pathway following DENV infection in vivo

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Fluid migration and fluid seepage in the Connemara Field, Porcupine Basin interpreted from industrial 3D seismic and well data combined with high-resolution site survey data

This study documents the suite of processes associated with source-to-seafloor fluid migration in the Connemara field area on the basis of 3D seismic data, well logs, 2D high-resolution seismic profiles, subbottom profiles, short cores and sidescan sonar data. The combination of datasets yields details about fluid migration pathways in the deep subsurface, in the unlithified shallow subsurface and about the distribution of fluid and gas seeps (pockmarks) at the sea floor. The Connemara field area is characterized by vertical fluid migration pathways (“seismic chimneys” or “gas chimneys”) that extend from the top of the Jurassic sequence, cross-cutting the entire Cretaceous sequence to the Upper Tertiary deposits over a vertical distance of up to 1.5 km. Their localization is mainly structurally controlled to the crest of tilted fault blocks along the main hydrocarbon migration pathways. These chimneys are important conduits for focused vertical fluid/gas flow from the deep to the shallow subsurface. However, gas seeps (pockmarks) at the sea floor are almost randomly distributed, which indicates a change from focused to diffuse fluid/gas migration in shallow, unconsolidated sediment. Where the vertical chimneys reach up to unlithified Eocene to Miocene sands, widespread deformation, interpreted as fluidization, occurs around the main conduit. This deformation affects about 32% of the entire unconsolidated Tertiary section (Late Eocene – Miocene). A Plio-Pleistocene glaciomarine drift with up to five horizons with iceberg ploughmarks seals the Tertiary sands. In the near surface sediments it is observed that gas accumulation occurs preferentially at iceberg ploughmarks. It is inferred that lateral migration at five levels of randomly oriented ploughmarks dispersed gas over larger areas and caused random pockmark distribution at the sea floor, independent from the underlying focused migration pathways. This study demonstrates that fluid flow migration changes from structurally controlled focused flow in the deep consolidated subsurface to diffuse flow, controlled by sediment variability, in the shallow subsurface. This result is relevant to a better understanding of the distribution of seepage-induced features at the seafloor related to focused hydrocarbon migration pathways known from industry data and fluid flow modeling

Ensuring leadership at the operational level of a health system in protracted crisis context: a cross-sectional qualitative study covering 8 health districts in Eastern Democratic Republic of Congo

Abstract Background This study examines how leadership is provided at the operational level of a health system in a protracted crisis context. Despite advances in medical science and technology, health systems in low- and middle-income countries struggle to deliver quality care to all their citizens. The role of leadership in fostering resilience and positive transformation of a health system is established. However, there is little literature on this issue in Democratic Republic of the Congo (DRC). This study describes leadership as experienced and perceived by health managers in crisis affected health districts in Eastern DRC. Methods A qualitative cross-sectional study was conducted in eight rural health districts (corresponding to health zones, in DRC’s health system organization), in 2021. Data were collected through in-depth interviews and non-participatory observations. Participants were key health actors in each district. The study deductively explored six themes related to leadership, using an adapted version of the Leadership Framework conceptual approach to leadership from the United Kingdom National Health Service’s Leadership Academy. From these themes, a secondary analysis extracted emerging subthemes. Results The study has revealed deficiencies regarding management and organization of the health zones, internal collaboration within their management teams as well as collaboration between these teams and the health zone’s external partners. Communication and clinical and managerial capacities were identified as key factors to be strengthened in improving leadership within the districts. The findings have also highlighted the detrimental influence of vertical interventions from external partners and hierarchical supervisors in health zones on planning, human resource management and decision-making autonomy of district leaders, weakening their leadership. Conclusions Despite their decentralized basic operating structure, which has withstood decades of crisis and insufficient government investment in healthcare, the districts still struggle to assert their leadership and autonomy. The authors suggest greater support for personal and professional development of the health workforce, coupled with increased government investment, to further strengthen health system capacities in these settings

Elaboration of Porous NiO/8YSZ Layers by Several SPS and SPPS Routes

International audienceSolution Precursor Plasma Spraying (SPPS) is a thermal spray process where a feedstock solution is heated and pyrolized to form fine (i.e., 1000 nm) molten particles that deposit onto a substrate to form a layer. The benefits of implementing the SPPS process include, among others: (i) the possibility to create unique microstructures at nanometer scale without the injection feeding problems usually associated to powder feeders and delivery cables and (ii) rapid exploration of novel precursor compositions. In this study, preparation and characterization of porous anode layers with homogeneous Nickel distribution and nanometer sized microstructure are considered for solid oxide fuel cell (SOFC) application. Once the solution is injected, the droplets go through several chemical and physical changes and impact the substrate in different states, from fully molten one to unpyrolized one. The effects of some spray parameters, such as the spray distance and the plasma flow mass enthalpy, on the layer architecture and composition were investigated. The results show that dense or porous layers can be manufactured depending on the operating parameters