Temporomandibular disorders, voice and oral quality of life in women

Some studies have shown a relationship between temporomandibular disorders (TMD) and dysphonia, as well as quality of life in oral health. OBJECTIVE: The purpose of this study was to investigate the correlation between severity of vocal self-perception and TMD severity and the correlation between oral health-related quality of life impairment and TMD severity. MATERIAL AND METHODS: Thirty-three women aged 20 to 40 years, with or without complaint of dysphonia, were recruited at the Bauru campus of the University of São Paulo, Brazil, and the local community. All participants were subjected to an investigation of quality of life related to dental and speech aspects by the application of Oral Health Impact Profile-short form (OHIP-14) and the Voice-Related Quality of Life (V-RQOL) protocol. Also, a questionnaire was applied to detect the presence and severity of TMD. RESULTS: There was significant correlation between TMD and quality of life for all aspects analyzed in the oral health protocol, except for function and physical limitation (p>0.05). There was negative correlation between TMD and voice-related quality of life in the total score (p=0.007) as weel as physical (p=0.008) and socio-emotional aspects (p=0.017). In addition, there was statistically significant correlation between TMD and vocal self-perception (p=0.037). CONCLUSION: There is an association between TMD severity, voice-related and oral health-related quality of life. It is important to investigate in future studies the vocal self perception as well as the oral and voice conditions in patients with TMD.(FAPESP) São Paulo Research Foundatio

Quantitative changes of nicotinic receptors in the hippocampus of dystrophin-deficient mice

Lack of dystrophin in Duchenne muscle dystrophy (DMD) and in the mutant mdx mouse results in progressive muscle degeneration, structural changes at the neuromuscular junction, and destabilization of the nicotinic acetylcholine receptors (nAChRs). One-third of DMD patients also present non-progressive cognitive impairments. Considering the role of the cholinergic system in cognitive functions, the number of nAChR binding sites and the mRNA levels of alpha 4, beta 2, and alpha 7 subunits were determined in brain regions normally enriched in dystrophin (cortex, hippocampus and cerebellum) of mdx mice using specific ligands and reverse-transcription polymerase chain reaction assays, respectively. Membrane preparations of these brain regions were obtained from male control and mdx mice at 4 and 12 months of age. the number of [H-3]-cytisine (alpha 4 beta 2) and [I-125]-alpha-bungarotoxin ([I-125]-alpha BGT, alpha 7) binding sites in the cortex and cerebellum was not altered with age or among age-matched control and mdx mice. A significant reduction in [H-3]-cytisine (48%) and [I-125]-alpha BGT (37%) binding sites was detected in the hippocampus of mdx mice at 12 months of age. When compared with the age-matched control groups, the mdx mice did not have significantly altered [H-3]-cytisine binding in the hippocampus, but [I-125]-alpha BGT binding in the same brain region was 52% higher at 4 months and 20% lower at 12 months. mRNA transcripts for the nAChR alpha 4, beta 2, and alpha 7 subunits were not significantly altered in the same brain regions of all animal groups. These results suggest a potential alteration of the nicotinic cholinergic function in the hippocampus of dystrophin-deficient mice, which might contribute to the impairments in cognitive functions, such as learning and memory, that have been reported in the dystrophic murine model and DMD patients. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Escola Paulista Med, Sect Nat Prod, Dept Pharmacol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Sect Expt Endocrinol, Dept Pharmacol, São Paulo, BrazilUniv Fed Santa Catarina, Dept Pharmacol, Neuropharmacol Lab, Florianopolis, SC, BrazilAmazon Biotechnol Ctr, Lab Pharmacol & Toxicol, Manaus, AM, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Sect Nat Prod, Dept Pharmacol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Sect Expt Endocrinol, Dept Pharmacol, São Paulo, BrazilWeb of Scienc

Butanolic extract of Aster squamatus aerial parts is the active fraction responsible to the antiulcer and gastric acid antisecretory effects

Aster squamatus es usado popularmente como antidiarreico, antineoplásico y cicatrizante. Los estudios anteriores demostraron un efecto antiulcerogénico del extracto hidroalcohólico crudo de la planta. Esto nos condujo a determinar las fracciones activas de este extracto para la actividad anti-ulcerogénica usando el modelo de úlcera inducido por etanol. Se determinó que la fracción BuOH del extracto hidroalcohólico crudo de las piezas aéreas es la responsable de la actividad anti-ulcerogénica. Esta fracción es también eficaz para inhibir la secreción ácida gástrica. Estudios posteriores deben ser llevados a cabo con esta fracción para intentar poner en evidencia el principio activo responsable.Aster squamatus is widespread used as antidiarrhoeic, antineoplasic and cicatrizing, and previous studies have revelead an antiulcer effect of crude hydroalcoholic extract of this plant. This led us to determine the active fraction(s) of this extract for antiulcer activity using the ethanol- induced ulcer model. The BuOH-precipitated part from the crude hydroalcoholic extract of aerial parts was determined to be the active fraction. This fraction was also effective to inhibit gastric acid secretion. Further studies should be conducted with the active fraction trying to elucidate the active principle.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Butanolic extract of Aster squamatus aerial parts is the active fraction responsible to the antiulcer and gastric acid antisecretory effects

Aster squamatus es usado popularmente como antidiarreico, antineoplásico y cicatrizante. Los estudios anteriores demostraron un efecto antiulcerogénico del extracto hidroalcohólico crudo de la planta. Esto nos condujo a determinar las fracciones activas de este extracto para la actividad anti-ulcerogénica usando el modelo de úlcera inducido por etanol. Se determinó que la fracción BuOH del extracto hidroalcohólico crudo de las piezas aéreas es la responsable de la actividad anti-ulcerogénica. Esta fracción es también eficaz para inhibir la secreción ácida gástrica. Estudios posteriores deben ser llevados a cabo con esta fracción para intentar poner en evidencia el principio activo responsable.Aster squamatus is widespread used as antidiarrhoeic, antineoplasic and cicatrizing, and previous studies have revelead an antiulcer effect of crude hydroalcoholic extract of this plant. This led us to determine the active fraction(s) of this extract for antiulcer activity using the ethanol- induced ulcer model. The BuOH-precipitated part from the crude hydroalcoholic extract of aerial parts was determined to be the active fraction. This fraction was also effective to inhibit gastric acid secretion. Further studies should be conducted with the active fraction trying to elucidate the active principle.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Analgesic and anti-inflammatory properties of Plantago australis hydroalcoholic extract

Fueron investigados los extractos hidroalcohólicos de hojas, raíces y frutos de Plantago australis Lam. (Planta-ginaceae) para determinar sus posibles propiedades antiinflamatorias y analgésicas. La inducción del edema de pata por carragenina fue significantemente inhibida (P<0,05) por la administración oral de estos extractos. También hubo una reducción en el número total de contorsiones inducidas por el ácido acético con todas las partes de la planta estudiadas. Los resultados indican que los extractos hidroalcohólico de hojas, raíces y frutos de P. australis estarían dotados de actividad antiinflamatória y analgésica.The hydroalcoholic extracts of leaves, roots, and fruits of Plantago australis Lam. (Plantagi-naceae) were investigated regarding their possible anti-inflammatory and analgesic properties. Car-rageenan-induced rat hind paw edema was significantly inhibited (P<0.05) by oral administration of these extracts. All studied extracts also reduced the total number of writhes induced by acetic acid. The results indicated that the hydroalcoholic extracts of leaves, roots, and fruits of P. australis are endowed with anti-inflammatory and analgesic activities.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Analgesic and anti-inflammatory properties of Plantago australis hydroalcoholic extract

Fueron investigados los extractos hidroalcohólicos de hojas, raíces y frutos de Plantago australis Lam. (Planta-ginaceae) para determinar sus posibles propiedades antiinflamatorias y analgésicas. La inducción del edema de pata por carragenina fue significantemente inhibida (P<0,05) por la administración oral de estos extractos. También hubo una reducción en el número total de contorsiones inducidas por el ácido acético con todas las partes de la planta estudiadas. Los resultados indican que los extractos hidroalcohólico de hojas, raíces y frutos de P. australis estarían dotados de actividad antiinflamatória y analgésica.The hydroalcoholic extracts of leaves, roots, and fruits of Plantago australis Lam. (Plantagi-naceae) were investigated regarding their possible anti-inflammatory and analgesic properties. Car-rageenan-induced rat hind paw edema was significantly inhibited (P<0.05) by oral administration of these extracts. All studied extracts also reduced the total number of writhes induced by acetic acid. The results indicated that the hydroalcoholic extracts of leaves, roots, and fruits of P. australis are endowed with anti-inflammatory and analgesic activities.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Analgesic and anti-inflammatory properties of Plantago australis hydroalcoholic extract

Fueron investigados los extractos hidroalcohólicos de hojas, raíces y frutos de Plantago australis Lam. (Planta-ginaceae) para determinar sus posibles propiedades antiinflamatorias y analgésicas. La inducción del edema de pata por carragenina fue significantemente inhibida (P<0,05) por la administración oral de estos extractos. También hubo una reducción en el número total de contorsiones inducidas por el ácido acético con todas las partes de la planta estudiadas. Los resultados indican que los extractos hidroalcohólico de hojas, raíces y frutos de P. australis estarían dotados de actividad antiinflamatória y analgésica.The hydroalcoholic extracts of leaves, roots, and fruits of Plantago australis Lam. (Plantagi-naceae) were investigated regarding their possible anti-inflammatory and analgesic properties. Car-rageenan-induced rat hind paw edema was significantly inhibited (P<0.05) by oral administration of these extracts. All studied extracts also reduced the total number of writhes induced by acetic acid. The results indicated that the hydroalcoholic extracts of leaves, roots, and fruits of P. australis are endowed with anti-inflammatory and analgesic activities.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Evaluation of glutathione S-Transferase GSTM1 and GSTT1 deletion polymorphisms on type-2 diabetes Mellitus risk

Submitted by Franciele Moreira ([email protected]) on 2017-07-07T13:37:56Z No. of bitstreams: 2 Artigo - Denise da Silva Pinheiro - 2013.pdf: 152173 bytes, checksum: 568f972b3dffe2cf77f0856d7fbc6054 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2017-07-11T13:41:00Z (GMT) No. of bitstreams: 2 Artigo - Denise da Silva Pinheiro - 2013.pdf: 152173 bytes, checksum: 568f972b3dffe2cf77f0856d7fbc6054 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2017-07-11T13:42:20Z (GMT) No. of bitstreams: 2 Artigo - Denise da Silva Pinheiro - 2013.pdf: 152173 bytes, checksum: 568f972b3dffe2cf77f0856d7fbc6054 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-07-11T13:42:29Z (GMT). No. of bitstreams: 2 Artigo - Denise da Silva Pinheiro - 2013.pdf: 152173 bytes, checksum: 568f972b3dffe2cf77f0856d7fbc6054 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2013-10Background: Due to the activity of GSTs in the detoxification of oxidative stress products, deletion polymorphisms of GSTM1 and GSTT1 may contribute to susceptibility to T2DM, since B-cells express very low levels of antioxidant enzymes. Recently, some studies have shown an association between GSTM1-null/GSTT1-null genotypes and an increased susceptibility to T2DM. A relationship between these polymorphisms and changes in the clinical parameters of diabetic patients has also been investigated. However, the results diverge considerably among the studies. Thus, this case-control study was designed to contribute to existing knowledge, as there are no studies on this issue performed in the Brazilian population. Methods and Findings: A total of 120 patients and 147 healthy individuals were included in this study. GSTT1 and GSTM1 deletion polymorphisms were genotyped by multiplex SYBR Green Real-Time PCR. The GSTT1-null genotype conferred a 3.2- fold increased risk to T2DM relative to the present genotype. There was no association between GSTM1-null and T2DM risk. In diabetic patients, GSTT1-null conferred higher levels of triglycerides and VLDL-cholesterol, while GSTM1-null was associated with increased levels of fasting blood glucose, glycated hemoglobin and blood pressure. We emphasized a necessity for applying log-linear analysis in order to explore an interaction between these polymorphisms properly. Conclusion: These results suggest that the GSTT1 polymorphism may play an important role in the pathogenesis of T2DM in the Brazilian population. This gene could then be added to a set of genetic markers to identify individuals with an increased risk for developing T2DM and complications associated with dyslipidemia in diabetic patients. Although there was no association of GSTM1 deletion polymorphism with susceptibility to T2DM, the influence of this polymorphism on important clinical parameters related to glycemia and blood pressure levels was verified. This finding suggests that both GSTM1-null and GSTT1-null may contribute to the clinical course of T2DM patients

Avaliação do nível de controle glicêmico dos pacientes diabéticos tipo 2 atendidos em um Hospital Universitário

Diabetes mellitus tipo 2 (DM2) é uma doença multifatorial, caracterizada pela hiperglicemia crônica associada à adoção de estilos de vida pouco saudáveis como o sedentarismo, dieta inadequada e, sobretudo a obesidade. O controle glicêmico constitui a principal meta no tratamento do paciente diabético para a prevenção do acometimento por diversas complicações clínicas, com destaque para a doença cardiovascular. Este trabalho caracteriza-se como um estudo de casos desenvolvido no Hospital das Clínicas da Universidade Federal de Goiás que teve como objetivo avaliar o nível de controle glicêmico e caracterizar o perfil dos pacientes portadores de DM2 acompanhados pelo serviço de endocrinologia. Foram avaliadas variáveis demográficas, clínicas e o de estilo de vida de 120 destes pacientes. Os dados obtidos permitem inferir uma maior proporção de pacientes do sexo feminino (69%), média de idade de 60,5 anos, tempo médio de acometimento pela doença de 14 anos, alta prevalência de pacientes com DM2 descompensado avaliado pela frequência de alterações na taxa de hemoglobina glicada, glicemia de jejum, IMC e perfil lipídico. Quanto ao estilo de vida dos pacientes foi verificado que apenas 4,2% permaneceram tabagistas após o diagnóstico da doença, no entanto, a maioria dos indivíduos tem dificuldade em seguir a dieta e praticar exercícios físicos regularmente. Estes achados apontam uma situação preocupante que demanda esforços no sentido de aumentar a adesão dos pacientes ao plano de tratamento, principalmente no que se refere a mudanças no estilo de vida