Flow of shale gas in tight rocks using a non-Linear transport model with pressure dependent model parameters

A new source of energy has recently been discovered from unconventional hydrocarbon reservoirs, such as shale gas deposits. Shale gas is found in tight porous rocks which are characterised by nano-scale size porous networks with ultra-low permeability [1,2]. The modelling of transport through such tight porous media is very challenging because it is a relatively new discipline and not much is known but transport processes in them, and little data is available; but it is a growing sector and must be addressed. Here, we apply a recently developed non-linear gas transport model [3], to reservoir simulations of single-phase gas through homogeneous tight rocks. Please download the full abstract below

Metrics With Vanishing Quantum Corrections

We investigate solutions of the classical Einstein or supergravity equations that solve any set of quantum corrected Einstein equations in which the Einstein tensor plus a multiple of the metric is equated to a symmetric conserved tensor $T_{\mu \nu}$ constructed from sums of terms the involving contractions of the metric and powers of arbitrary covariant derivatives of the curvature tensor. A classical solution, such as an Einstein metric, is called {\it universal} if, when evaluated on that Einstein metric, $T_{\mu \nu}$ is a multiple of the metric. A Ricci flat classical solution is called {\it strongly universal} if, when evaluated on that Ricci flat metric, $T_{\mu \nu}$ vanishes. It is well known that pp-waves in four spacetime dimensions are strongly universal. We focus attention on a natural generalisation; Einstein metrics with holonomy ${\rm Sim} (n-2)$ in which all scalar invariants are zero or constant. In four dimensions we demonstrate that the generalised Ghanam-Thompson metric is weakly universal and that the Goldberg-Kerr metric is strongly universal; indeed, we show that universality extends to all 4-dimensional ${\rm Sim}(2)$ Einstein metrics. We also discuss generalizations to higher dimensions.Comment: 23 page

On the local structure of Lorentzian Einstein manifolds with parallel distribution of null lines

We study transformations of coordinates on a Lorentzian Einstein manifold with a parallel distribution of null lines and show that the general Walker coordinates can be simplified. In these coordinates, the full Lorentzian Einstein equation is reduced to equations on a family of Einstein Riemannian metrics.Comment: Dedicated to Dmitri Vladimirovich Alekseevsky on his 70th birthda

Holonomy of Einstein Lorentzian manifolds

The classification of all possible holonomy algebras of Einstein and vacuum Einstein Lorentzian manifolds is obtained. It is shown that each such algebra appears as the holonomy algebra of an Einstein (resp., vacuum Einstein) Lorentzian manifold, the direct constructions are given. Also the holonomy algebras of totally Ricci-isotropic Lorentzian manifolds are classified. The classification of the holonomy algebras of Lorentzian manifolds is reviewed and a complete description of the spaces of curvature tensors for these holonomies is given.Comment: Dedicated to to Mark Volfovich Losik on his 75th birthday. This version is an extended part of the previous version; another part of the previous version is extended and submitted as arXiv:1001.444

Archaeometric Study of the Historic Terrazzo Pavement of Prince Mohamed Ali Museum, Cairo, Egypt

This research will shed light on studying a terrazzo pavement in Prince Mohamed Ali Museum (the case study). The authors used visual inspection, stereo microscope, USB microscope, XRPD analysis, and SEM.EDX to identify its components, deterioration aspects and execution techniques. The XRPD and SEM.EDX results revealed that Portland cement was used in the three layers of terrazzo because of the detection of Hatrurite, Alite, Anorthite, Albite, Aragonite, etc. Many pigments were used in the topping terrazzo layer as; Goethite, Greenalite, Hematite, Azurite and Magnetite. The divider strips were made of brass alloy and the topping layer chips were prepared from basalt, marble and sea shells

The aortic root in repaired tetralogy of Fallot:Serial measurements and impact of losartan treatment

Background: Aortic root dilatation is common in adults with repaired tetralogy of Fallot (rTOF) and might lead to aortic dissection. However, little is known on progression of aortic dilatation and the effect of pharmaceutical treatment. This study aims to determine factors associated with aortic growth and investigate effects of losartan. Methods and results: We performed a prespecified analysis from the 1:1 randomized, double-blind REDEFINE trial. Aortic root diameters were measured at baseline and after 2.0 ± 0.3 years of follow-up using cardiovascular magnetic resonance (CMR) imaging. A total of 66 patients were included (68% men, age 40 ± 12 years, baseline aortic root 37 ± 6 mm, 32% aortic dilatation (>40 mm)). There was a trend towards slow aortic root growth (+0.6 ± 2.3 mm after two years, p = 0.06) (n = 60). LV stroke volume was the only factor associated with both a larger baseline aortic root (β: 0.09 mm/ml (95% C.I.:0.02, 0.15), p = 0.010) and with aortic growth during follow-up (β: 0.04 mm/ml (95% C.I.:0.005, 0.066), p = 0.024), after correction for age, sex, and body surface area using linear regression analysis. No treatment effect of losartan was found (p = 0.17). Conclusions: Aortic root dilatation was present in about one-third of rTOF patients. A larger LV stroke volume was associated with both a larger baseline aortic root and ongoing growth. Our findings provide no arguments for lower aortic diameter thresholds for prophylactic surgery compared to the general population

Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer

SIMPLE SUMMARY: Small extracellular vesicles (sEVs) released by all cell types function as a mediator in intercellular communication that can promote cell division and survival to remodel the tumor microenvironment to develop tumor invasion and metastasis. Even though dsDNA baggage is associated with all small EV populations, the functional role of EV-DNA in cancer remains poorly understood. This is due to a lack of methods allowing the efficient separation of small EVs (sEVs) from other non-sEV components. The main aim of our study was to develop an efficient sEV isolation method along with EV-associated DNA (EV-DNA) monitoring tool to evaluate the role of EV-DNA as a mediator of cell–cell communication in cancer. Our detailed small EV-DNA characterization confirmed that isolated sEVs using the TSU method (Tangential flow filtration + Size exclusion chromatography + Ultrafiltration) are free from contaminants such as cell-free and apoptotic bodies DNA, making TSU ideal for performing EV-DNA functional studies. Next, we revealed the exact EV-DNA distribution in the recipient cells using 3D image analysis and the association of EV-DNA with key cellular proteins, which may have an essential role in cancer. In the leukemia model, EV-DNA isolated from leukemia cell lines associated with mesenchymal stromal cells (MSCs), a crucial factor in the bone marrow (BM) microenvironment. ABSTRACT: Small extracellular vesicles (sEVs) play essential roles in intercellular signaling both in normal and pathophysiological conditions. Comprehensive studies of dsDNA associated with sEVs are hampered by a lack of methods, allowing efficient separation of sEVs from free-circulating DNA and apoptotic bodies. In this work, using controlled culture conditions, we enriched the reproducible separation of sEVs from free-circulated components by combining tangential flow filtration, size-exclusion chromatography, and ultrafiltration (TSU). EV-enriched fractions (F2 and F3) obtained using TSU also contained more dsDNA derived from the host genome and mitochondria, predominantly localized inside the vesicles. Three-dimensional reconstruction of high-resolution imaging showed that the recipient cell membrane barrier restricts a portion of EV-DNA. Simultaneously, the remaining EV-DNA overcomes it and enters the cytoplasm and nucleus. In the cytoplasm, EV-DNA associates with dsDNA-inflammatory sensors (cGAS/STING) and endosomal proteins (Rab5/Rab7). Relevant to cancer, we found that EV-DNA isolated from leukemia cell lines communicates with mesenchymal stromal cells (MSCs), a critical component in the BM microenvironment. Furthermore, we illustrated the arrangement of sEVs and EV-DNA at a single vesicle level using super-resolution microscopy. Altogether, employing TSU isolation, we demonstrated EV-DNA distribution and a tool to evaluate the exact EV-DNA role of cell–cell communication in cancer

Seroepidemiology of Toxoplasma gondii infection in patients with liver disease in eastern China

The role of the protozoan parasite Toxoplasma gondii in the pathogenesis of liver disease has recently gained much interest. The aim of this study was to determine the prevalence and risk factors associated with T. gondii infection in patients with liver disease from three cities in Shandong and Henan provinces, China. A case–control study was conducted from December 2014 to November 2015 and included 1142 patients with liver disease and 1142 healthy controls. Serum samples were collected from all individuals and were examined with enzyme-linked immunosorbent assay for the presence of anti-T. gondii IgG and IgM antibodies. Information on the demographics, clinical, and lifestyle characteristics of the participants was collected from the medical records and by the use of a questionnaire. The prevalence of anti-T. gondii IgG was 19·7% in patients with liver disease compared with 12·17% in the controls. Only 13 patients had anti-T. gondii IgM antibodies compared with 12 control individuals (1·14% vs. 1·05%, respectively). The highest seroprevalence was detected in patients with liver cancer (22·13%), followed by hepatitis patients (20·86%), liver cirrhosis patients (20·42%), and steatosis patients (20%). Multivariate logistic regression analysis indicated that consumption of raw meat (odds ratio (OR) = 1·32; 95% confidence interval (CI) 1·01–1·71; P = 0·03) and source of drinking water from wells (OR = 1·56; 95% CI 1·08–2·27; P = 0·01) were independent risk factors for T. gondii infection in liver disease patients. These findings indicate that T. gondii infection is more likely to be present in patients with liver disease. Therefore, efforts should be directed toward health education of populations at high risk of T. gondii infection and measures should be taken to protect vulnerable patients with liver disease