208 research outputs found
Burial and Origin of Permafrost Derived Carbon in the Nearshore Zone of the Southern Canadian Beaufort Sea
Membrance interface evaluations for underwater mass spectrometers.
A component that has enabled the development of underwater mass spectrometry is a
mechanically supported membrane interface probe. Our two research groups have used
metallic porous frits that support polydimethyl siloxane (PDMS) membranes embedded in a
heated membrane probe assembly, allowing the deployment of the underwater membrane
introduction mass spectrometer (MIMS) instruments to ocean depths of 2000 meters. The
fabrication of such frits has consisted of shaping larger Hastalloy C porous frits to the size
required to support a PDMS capillary of 0.64 mm ID and 1.19 mm OD using a diamondâcoated
wheel and Dremel tool. This procedure is timeâconsuming and cumbersome, and the porosity
of the final frits is likely not reproducible. To facilitate the fabrication of the membrane
assembly, we report on the use of new porous metallic structures. Frits with diameters of
approximately 3.0 mm (1/8â) and known porosities (48.3 % and 32.5%) were produced by the
Fraunhofer Institute in Dresden, Germany, using powder metallurgical processes. We used
these frits to fabricate new membrane interface assemblies. Using a new customâheated
membrane probe with the new porous frits, we performed calibrations relating dissolved
methane concentrations to mass spectrometer response (m/z 15) using linear leastâsquares
fitting procedures. Both the limit of detection (methane concentration in the tens of
nanomolars) and the sensitivity (on the order of 10â1 picoâamps/nanomole of methane) were
found to be comparable with those obtained with the previously fabricated Hastelloy C frits.
The calibration parameters for the new assembly were also found to be a function of the flow
rate, temperature, and sample hydrostatic pressure
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Phasor Measurement Units Optimal Placement and Performance Limits for Fault Localization
In this paper, the performance limits of faults localization are investigated using synchrophasor data. The focus is on a non-trivial operating regime where the number of Phasor Measurement Unit (PMU) sensors available is insufficient to have full observability of the grid state. Proposed analysis uses the Kullback Leibler (KL) divergence between the distributions corresponding to different fault location hypotheses associated with the observation model. This analysis shows that the most likely locations are concentrated in clusters of buses more tightly connected to the actual fault site akin to graph communities. Consequently, a PMU placement strategy is derived that achieves a near-optimal resolution for localizing faults for a given number of sensors. The problem is also analyzed from the perspective of sampling a graph signal, and how the placement of the PMUs i.e. the spatial sampling pattern and the topological characteristic of the grid affect the ability to successfully localize faults. To highlight the superior performance of presented fault localization and placement algorithms, the proposed strategy is applied to a modified IEEE 34, IEEE-123 bus test cases and to data from a real distribution grid. Additionally, the detection of cyber-physical attacks is also examined where PMU data and relevant Supervisory Control and Data Acquisition (SCADA) network traffic information are compared to determine if a network breach has affected the integrity of the system information and/or operations
Universality of residence-time distributions in non-adiabatic stochastic resonance
We present mathematically rigorous expressions for the residence-time and
first-passage-time distributions of a periodically forced Brownian particle in
a bistable potential. For a broad range of forcing frequencies and amplitudes,
the distributions are close to periodically modulated exponential ones.
Remarkably, the periodic modulations are governed by universal functions,
depending on a single parameter related to the forcing period. The behaviour of
the distributions and their moments is analysed, in particular in the low- and
high-frequency limits.Comment: 8 pages, 1 figure New version includes distinction between
first-passage-time and residence-time distribution
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Seasonal methane accumulation and release from a gas emission site in the central North Sea
We investigated dissolved methane distributions along a 6 km transect crossing active seep sites at 40 m water depth in the central North Sea. These investigations were done under conditions of thermal stratification in summer (July 2013) and homogenous water column in winter (January 2014). Dissolved methane accumulated below the seasonal thermocline in summer with a median concentration of 390 nM, whereas during winter, methane concentrations were typically much lower (median concentration of 22 nM). High-resolution methane analysis using an underwater mass-spectrometer confirmed our summer results and was used to document prevailing stratification over the tidal cycle. We contrast estimates of methane oxidation rates (from 0.1 to 4.0 nM dayâ»Âč) using the traditional approach scaled to methane concentrations with microbial turnover time values and suggest that the scaling to concentration may obscure the ecosystem microbial activity when comparing systems with different methane concentrations. Our measured and averaged rate constants (k') were on the order of 0.01 dayâ1, equivalent to a turnover time of 100 days, even when summer stratification led to enhanced methane concentrations in the bottom water. Consistent with these observations, we could not detect known methanotrophs and pmoA genes in water samples collected during both seasons. Estimated methane fluxes indicate that horizontal transport is the dominant process dispersing the methane plume. During periods of high wind speed (winter), more methane is lost to the atmosphere than oxidized in the water. Microbial oxidation seems of minor importance throughout the year.This work is part of the DFG project âLimitations of Marine Methane Oxidationâ (MA 3961/2-1). This is the publisherâs final pdf. The published article is copyrighted by the author(s) and published by Copernicus Publications on behalf of the European Geosciences Union. The published article can be found at: http://www.biogeosciences.net/ For a previous discussion paper please see: http://hdl.handle.net/1957/5749
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Seasonal methane accumulation and release from a gas emission site in the central North Sea
Hydroacoustic data document the occurrence of 5 flare clusters and several single flares from which bubbles rise through the entire water column from an active seep site at 40 m water depth in the central North Sea. We investigated the difference in dissolved methane distributions along a 6 km transect crossing this seep site during a period of seasonal summer stratification (July 2013) and a period of well mixed winter water column (January 2014). Dissolved methane accumulated below the seasonal thermocline in summer with a median concentration of 390 nM, whereas during winter, methane concentrations were much lower (median concentration of 22 nM) and punctually elevated due to bubble transport. High resolution methane analysis by an underwater mass-spectrometer confirmed our summer results and were used to document prevailing stratification over the tidal cycle. Although sufficient methane was available, microbial methane oxidation was limited during both seasons. Measured and averaged rate constants (k') using Michaelis Menten kinetics were on the order of 0.01 daysâ»Âč, equivalent to a turnover time of 100 days. Time series measurements indicated an uptake of only 5â6% of the gas after 4 days, and no known methanotrophs and pmoA-genes were detected. Estimated methane fluxes indicate that horizontal eddy transport rapidly disperses dissolved methane, vertical transport becomes dominant during phases of high wind speeds, and relative to these processes, microbial methane oxidation appears to be comparably low. To bridge the discrete field data we developed a 1-D seasonal model using available year-long records of wind speed, surface temperature and thermocline depth. The model simulations show a peak release of methane at the beginning of fall when the water column becomes mixed. Consistent with our field data, inclusion of microbial methane oxidation does not change the model results significantly, thus microbial oxidation appears to be not sufficient to notably reduce methane during summer stratification before the peak release in fall.This work is part of the DFG project âLimitations of Marine Methane Oxidationâ (MA 3961/2-1). This discussion paper has been under review for the journal Biogeosciences (BG). Please refer to the corresponding final paper in BG. The published article is copyrighted by the author(s) and published by Copernicus Publications on behalf of the European Geosciences Union. The published article can be found at: http://www.biogeosciences.net/ The final revised paper is available at: http://hdl.handle.net/1957/5749
An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc). Here we describe the construction of improved antibiotic-resistance cassettes that specify resistance to kanamycin, chloramphenicol or trimethoprim effectively in the Bcc and related species. These were then used in combination with and/or to construct a series enhanced suicide vectors, pSHAFT2, pSHAFT3 and pSHAFT-GFP to facilitate effective allelic replacement in the Bcc. Validation of these improved suicide vectors was demonstrated by the genetic inactivation of selected genes in the Bcc species Burkholderia cenocepacia and B. lata, and in the non-Bcc species, B. thailandensis
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