Infrared regularization with spin-3/2 fields

We present a Lorentz-invariant formulation of baryon chiral perturbation theory including spin-3/2 fields. Particular attention is paid to the projection on the spin-3/2 components of the delta fields. We also discuss the nucleon mass and the pion-nucleon sigma term.Comment: 9 pp, 1 fi

Plant-Derived Catechols Are Substrates of TonB-Dependent Transporters and Sensitize Pseudomonas aeruginosa to Siderophore-Drug Conjugates

Pseudomonas aeruginosa is an opportunistic pathogen responsible for acute and chronic infections in immunocompromised hosts. This organism is known to compete efficiently against coinfecting microorganisms, due in part to the secretion of antimicrobial molecules and the synthesis of siderophore molecules with high affinity for iron. P. aeruginosa possess a large repertoire of TonB-dependent transporters for the uptake of its own, as well as xenosiderophores released from other bacteria or fungi. Here, we show that P. aeruginosa is also capable of utilizing plant-derived polyphenols as an iron source. We found that exclusively plant-derived phenols containing a catechol group (i.e., chlorogenic acid, caffeic acid, quercetin, luteolin) induce the expression of the TonB-dependent transporters PiuA or PirA. This induction requires the two-component system PirR-PirS. Chlorogenic acid in its Fe(III)-loaded form was actively transported by PiuA and PirA and supported growth under iron-limiting conditions. Coincidentally, PiuA and PirA are also the main TonB transporters for the recently approved siderophore-drug conjugate cefiderocol. Surprisingly, quercetin supplementation increased the susceptibility of P. aeruginosa to siderophore-drug conjugates, due to induction of; piuA; and; pirA; expression mediated by the PirR-PirS two-component system. These findings suggest a potential novel therapeutic application for these biologically active dietary polyphenols.; IMPORTANCE; Iron is an essential element for living organisms. Most bacteria synthesize species-specific iron chelators, called siderophores, able to capture iron from their host or the environment. Pseudomonas aeruginosa, an opportunistic pathogen, produces two endogenous siderophores but is able to acquire iron also via xenosiderophores, produced by other bacteria or fungi, using a set of conserved TonB transporters. Here, we show that P. aeruginosa is also able to use plant metabolites, like quercetin and chlorogenic acid, as siderophores. These metabolites possess an iron-chelating catechol group and are recognized and transported by the TonB transporters PirA and PiuA. Since these transporters also promote the specific uptake of siderophore-drug conjugates, P. aeruginosa exposed to these plant catechols becomes hypersusceptible to this novel class of antibiotics. This unexpected finding suggests a potential therapeutic application for quercetin and chlorogenic acid, which were mainly investigated for their antioxidant and anti-inflammatory properties

Chiral Extrapolations and the Covariant Small Scale Expansion

We calculate the nucleon and the delta mass to fourth order in a covariant formulation of the small scale expansion. We analyze lattice data from the MILC collaboration and demonstrate that the available lattice data combined with our knowledge of the physical values for the nucleon and delta masses lead to consistent chiral extrapolation functions for both observables up to fairly large pion masses. This holds in particular for very recent data on the delta mass from the QCDSF collaboration. The resulting pion-nucleon sigma term is sigma_{piN} = 48.9 MeV. This first quantitative analysis of the quark-mass dependence of the structure of the Delta(1232) in full QCD within chiral effective field theory suggests that (the real part of) the nucleon-delta mass-splitting in the chiral limit, Delta_0 = 0.33 GeV, is slightly larger than at the physical point. Further analysis of simultaneous fits to nucleon and delta lattice data are needed for a precision determination of the properties of the first excited state of the nucleon.Comment: 11 pp, 2 figs, version accepted for publication in Phys. Lett.

Trojan Horse Siderophore Conjugates Induce Pseudomonas aeruginosa Suicide and Qualify the TonB Protein as a Novel Antibiotic Target

Rising infection rates with multidrug-resistant pathogens calls for antibiotics with novel modes of action. Herein, we identify the inner membrane protein TonB, a motor of active uptake in Gram-negative bacteria, as a novel target in antimicrobial therapy. The interaction of the TonB box of outer membrane transporters with TonB is crucial for the internalization of essential metabolites. We designed TonB box peptides and coupled them with synthetic siderophores in order to facilitate their uptake into bacteria in up to 32 synthetic steps. Three conjugates repressed the growth of Pseudomonas aeruginosa cells unable to produce their own siderophores, with minimal inhibitory concentrations between 0.1 and 0.5 μM. The transporters mediating uptake of these compounds were identified as PfeA and PirA. The study illustrates a variant of cellular suicide where a transporter imports its own inhibitor and demonstrates that artificial siderophores can import cargo with molecular weights up to 4 kDa

Consistent Calculation of the Nucleon Electromagnetic Polarizabilities in Chiral Perturbation Theory Beyond Next-to-Leading Order

We calculate the nucleons' electromagnetic polarizabilities in heavy baryon chiral perturbation theory including all terms to order ${\cal O} (q^4)$. The chiral prediction of the electric polarizabilities for the neutron and the proton are in good agreement with the data. In the case of the magnetic polarizabilities the big positive contribution from the $\Delta(1232)$ resonance is largely cancelled by a non--analytic loop contribution of the $\ln M_\pi$ type. This novel effect helps to understand the rather small empirical value of the nucleons' magnetic polarizability.Comment: 10 pp, TeX, BUTP-93/22 and CRN-93-3

The nucleon axial-vector coupling beyond one loop

We analyze the nucleon axial-vector coupling to two loops in chiral perturbation theory. We show that chiral extrapolations based on this representation require lattice data with pion masses below 300 MeV.Comment: 9 pp, 2 fig

Dispersive representation and shape of the Kl3 form factors: robustness

An accurate low-energy dispersive parametrization of the scalar Kpi form factor was constructed some time ago in terms of a single parameter guided by the Callan-Treiman low-energy theorem. A similar twice subtracted dispersive parametrization for the vector Kpi form factor will be investigated here. The robustness of the parametrization of these two form factors will be studied in great detail. In particular the cut-off dependence, the isospin breaking effects and the possible, though not highly probable, presence of zeros in the form factors will be discussed. Interesting constraints in the latter case will be obtained from the soft-kaon analog of the Callan-Treiman theorem and a comparison with the recent tau --> K pi nu_tau data.Comment: 24 pages, 11 figure

Chiral Extrapolation of the Strangeness Changing K pi Form Factor

We perform a chiral extrapolation of lattice data on the scalar K pi form factor and the ratio of the kaon and pion decay constants within Chiral Perturbation Theory to two loops. We determine the value of the scalar form factor at zero momentum transfer, at the Callan-Treiman point and at its soft kaon analog as well as its slope. Results are in good agreement with their determination from experiment using the standard couplings of quarks to the W boson. The slope is however rather large. A study of the convergence of the chiral expansion is also performed.Comment: few minor change

Ordinary and radiative muon capture on the proton and the pseudoscalar form factor of the nucleon

We calculate ordinary and radiative muon capture on the proton in an effective field theory of pions, nucleons and delta isobars, working to third and second order in the small scale expansion respectively. Preceding calculations in chiral effective field theories only employed pion and nucleon degrees of freedom and were not able to reproduce the photon spectrum in the pioneering experiment of radiative muon capture on the proton from TRIUMF. For the past few years it has been speculated that the discrepancy between theory and experiment might be due to Delta(1232) related effects, which are only included via higher order contact interactions in the standard chiral approach. In this report we demonstrate that this speculation does not hold true. We show that contrary to expectations from naive dimensional analysis isobar effects on the photon spectrum and the total rate in radiative muon capture are of the order of a few percent, consistent with earlier findings in a more phenomenological approach. We further demonstrate that both ordinary and radiative muon capture constitute systems with a very well behaved chiral expansion, both in standard chiral perturbation theory and in the small scale expansion, and present some new ideas that might be at the bottom of the still unresolved discrepancy between theory and experiment in radiative muon capture. Finally we comment upon the procedure employed by the TRIUMF group to extract new information from their radiative muon capture experiment on the pseudoscalar form factor of the nucleon. We show that it is inconsistent with the ordinary muon capture data.Comment: 22 pp, RevTeX, uses epsf, 8 figs, enlarged version, discussion of ordinary muon capture on protons substantially enlarged, accepted for publication in Nucl.Phys.A, FZJ-IKP(TH)-2000-0

The Delta-resonance in a finite volume

We study the extraction of Delta-resonance parameters from lattice data for small quark masses, corresponding to the case of an unstable Delta. To this end, we calculate the spectrum of the correlator of two Delta-fields in a finite Euclidian box up-to-and-including O(epsilon^3) in the small scale expansion using infrared regularization. On the basis of our numerical study, we argue that the extraction of the parameters of the Delta-resonance (in particular, of the mass and the pion-nucleon-delta coupling constant) from the measured volume dependence of the lowest energy levels should be feasible.Comment: 20 pages, 8 postscript figure