Popularizing Spanish Cancioneros in Rome (II): Ms. Ottoboniano 2882

In queste pagine si descrive brevemente il Ms. Ottoboniano 2882 della Biblioteca Vaticana. È un canzoniere manoscritto copiato intorno al 1580 che contiene numerosi testi di lirica spagnola tradizionale, interessante da studiare per approfondire il tema della diffusione della poesia tradizionale in Italia durante il xvi e xvii secolo.In these pages we briefly describe manuscript 2882 preserved in the Ottoboniana collection of the Vatican Library. It is a Spanish cancionero copied in about 1580 which contains many Spanish traditional lyric texts, and is very interesting for the analysis of the dissemination of Spanish traditional poetry in Italy during the 16th and 17th centuries.Il presente lavoro si inserisce nell’ambito del progetto PRIN 2012, Canzonieri spagnoli tra Rinascimento e Barocco (prot. 2012H7X9SX), coordinato da Antonio Gargano, in particolare nella ricerca dell’unità di Roma «La Sapienza» su Canzonieri spagnoli popolareggianti conservati a Roma, coordinata da Patrizia Botta (partecipanti: Aviva Garribba, Massimo Marini, Debora Vaccari; membri esterni: M. Teresa Cacho Palomar e Francesco Zimei)

Determination of Endogenous and Synthetic Glucocorticoids in Human Urine by Gas Chromatography – Mass Spectrometry Following Microwave Assisted Derivatization

Acomplete screening and confirmation analytical method for the direct determination of six endogenous (cortisol, cortisone, deoxycorticosterone, tetrahydrocortisol, tetrahydrocortisone, tetrahydro-S) and 17 synthetic (amcinonide, betamethasone, desoximethasone, dexamethasone, fludrocortisone, flumethasone, flunisolide, flucinolone acetonide, flucinonide, fluprednisolone, flurandrenolide, fluorometholone, 6-methylprednisolone, prednisolone, prednisone, triamcinolone, triamcinolone acetonide) glucocorticoids in human urine by gas chromatography with mass spectrometric detection (GC–MS) is presented. The analytical technique comprises a pre treatment procedure and the instrumental analysis of the trimethylsilyl (TMS) derivatives, performed by GC–MS (quadrupole) with electron impact (EI) ionization. The derivatization yields obtained by two different derivatizing mixtures, namely N-methyl N(trimethylsilyl)trifluoroacetamide (MTSFA):NH4I:dithioerythritol (DTE) 1000:2:4 (usually indicated as TMSiodine); and N-trimethylsilylimidazole (TMSim):N,O-bis(trimethylsilyl)acetamide (BSA):trimethylchlorosilane (TMCS) 3:3:2, both under direct thermal heating and with microwave (MW) irradiation, were evaluated, also as a function of the temperature, of the MWì power and of the incubation time. The highest yields of the derivatization process were obtained, for most of the compounds here considered, by a two-step procedure: a microwave-assisted derivatization stage (40 min in a microwave oven at 900Wemitted power), followed by a traditional heat transfer derivatization (1.5 h in a thermostated bath at 70 ◦C) with the derivatization mixture TMSim:BSA:TMCS 3:3:2. In these operating conditions, diagnostic EI–MS spectra of all considered glucocorticoids were obtained. Limits of detection (LOD) of synthetic glucocorticoids in urine ranged from 3 to 25g/l. The effectiveness of the method for the determination of glucocorticoids in urine was evaluated on spiked urine samples and on real samples obtained from patients under pharmacological treatment with synthetic glucocorticoids. Apart from the clinical monitoring of glucocorticoids in urine, the method can be applied as a complete screening + confirmation analytical protocol in antidoping tests for the detection of illicit administration of glucocorticoids by the athletes

End-to-end thiocyanato-bridged helical chain polymer and dichlorido-bridged copper(II) complexes with a hydrazone ligand: synthesis, characterisation by electron paramagnetic resonance and variable- temperature magnetic studies, and inhibitory effects on human colorectal carcinoma cells

The reactions of the tridentate hydrazone ligand, N’-[1-(pyridin-2-yl)ethylidene]acetohydrazide (HL), obtained by condensation of 2-acetylpyridine with acetic hyadrazide, with copper nitrate trihydrate in the presence of thiocyanate, or with CuCl2 produce two distinct coordination compounds, namely a one-dimensional helical coordination chain of [CuL(NCS)]n (1) units, and a doubly chlorido-bridged dinuclear complex [Cu2L2Cl2] (2) (where L=CH3C(O)=N − N=CCH3C5H4N). Single-crystal X-ray structural determination studies reveal that in complex 1, a deprotonated hydrazone ligand L- coordinates a copper(II) ion that is bridged to two neighbouring metal centres by SCN- anions, generating a one-dimensional helical coordination chain. In complex 2, two symmetry-related, adjacent copper(II) coordination entities are doubly chlorido-bridged, producing a dicopper entity with a Cu···Cu distance of 3.402 (1). The two coordination compounds have been fully characterised by elemental analysis, spectroscopic techniques including IR, UV– vis and electron paramagnetic resonance, and variable-temperature magnetic studies. The biological effects of 1 and 2 on the viability of human colorectal carcinoma cells (COLO-205 and HT-29) were evaluated using an MTT assay, and the results indicate that these complexes induce a decrease in cell-population growth of human colorectal carcinoma cells with apoptosis

Validation and Applications of Protein-Ligand Docking Approaches Improved for Metalloligands with Multiple Vacant Sites

Altres ajuts: COST Action CM1306Decoding the interaction between coordination compounds and proteins is of fundamental importance in biology, pharmacy, and medicine. In this context, protein-ligand docking represents a particularly interesting asset to predict how small compounds could interact with biomolecules, but to date, very little information is available to adapt these methodologies to metal-containing ligands. Here, we assessed the predictive capability of a metal-compatible parameter set for the docking program GOLD for metalloligands with multiple vacant sites and different geometries. The study first presents a benchmark of 25 well-characterized X-ray metalloligand-protein adducts. In 100% of the cases, the docking solutions are superimposable to the X-ray determination, and in 92% the value of the root-mean-square deviation between the experimental and calculated structures is lower than 1.5 Å. After the validation step, we applied these methods to five case studies for the prediction of the binding of pharmacological active metal species to proteins: (i) the anticancer copper(II) complex [Cu II (Br)(2-hydroxy-1-naphthaldehyde benzoyl hydrazine)(indazole)] to human serum albumin (HSA); (ii) one of the active species of antidiabetic and antitumor vanadium compounds, V IV O 2+ ion, to carboxypeptidase; (iii) the antiarthritic species [Au I (PEt 3 )] + to HSA; (iv) the antitumor oxaliplatin to ubiquitin; (v) the antitumor ruthenium(II) compound RAPTA-PentaOH to cathepsin B. The calculations suggested that the binding modes are in good agreement with the partial information retrieved from spectroscopic and spectrometric analysis and allowed us, in certain cases, to propose additional hypotheses. This method is an important update in protein-metalloligand docking, which could have a wide field of application, from biology and inorganic biochemistry to medicinal chemistry and pharmacology

Texts of Ms. Corsini 625 Disseminated in Italy

El Ms. Corsini 625 es un Cancionero musical español formado por treinta y tres poemas, en su mayoría de carácter tradicional, y fue compuesto a comienzos del XVII en la corte de un príncipe romano. Es uno de los frutos de la moda de la guitarra española que contribuyó a difundir la poesía española por las cortes de Italia. Este Manuscrito es, por un lado, portador de muchos unica (alguno de los cuales compuestos sin duda en la corte misma) y, por el otro, de varios poemas que están difundidos también en otras fuentes conservadas en Italia. Se analizan en particular aquellos poemas que están atestiguados solamente en Italia.The manuscript Corsini 625 is a Spanish songbook made up of thirty-three poems, most of which are traditional in character. This songbook was composed at the beginning of the 17th century in the court of a Roman prince. It is one of the fruits of the popularity of the Spanish guitar, which contributed to spread Spanish poetry through the courts of Italy. This manuscript contains, both many single witness poems (some of which are undoubtedly composed in the court itself) and poems that are also disseminated in other sources preserved in Italy. In this article, these poems are analyzed as well as others that are not known outside Italy.Este trabajo se enmarca en el Proyecto de investigación PRIN 2012 Canzonieri spagnoli tra Rinascimento e Barocco (prot. 2012H7X9SX), dirigido por Antonio Gargano

Aspectos léxicos de la traducción del Canzoniere por Enrique Garcés (1591)

Enrique Garcés’ translation of Petrarch’s Canzoniere is a late product of the dissemination of Petrarquism in the Hispanic world. The author is a Portuguese by birth who spent almost all his life in Peru. The vo c a bu l a ry of his translation hardly reveals the translator’s origin while it is full of Italianisms. Some of these words had already been introduced in the Spanish lexicon through the poetic language, others were less known. In addition, his translation includes calques and rather daring borrowings from Italian. The article contains the analysis of the translation of some key-words of Petrarch’s Can -zoniere

Influence of temperature on the equilibria of oxidovanadium(IV) complexes in solution.

The equilibria at different temperatures of VIVOL2complexes were investigated in order to elucidate their interaction with proteins