The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris

<p>Abstract</p> <p>Background</p> <p>Over recent years, a growing effort has been made to develop microsatellite markers for the genomic analysis of the common bean (<it>Phaseolus vulgaris</it>) to broaden the knowledge of the molecular genetic basis of this species. The availability of large sets of expressed sequence tags (ESTs) in public databases has given rise to an expedient approach for the identification of SSRs (Simple Sequence Repeats), specifically EST-derived SSRs. In the present work, a battery of new microsatellite markers was obtained from a search of the <it>Phaseolus vulgaris </it>EST database. The diversity, degree of transferability and polymorphism of these markers were tested.</p> <p>Results</p> <p>From 9,583 valid ESTs, 4,764 had microsatellite motifs, from which 377 were used to design primers, and 302 (80.11%) showed good amplification quality. To analyze transferability, a group of 167 SSRs were tested, and the results showed that they were 82% transferable across at least one species. The highest amplification rates were observed between the species from the <it>Phaseolus </it>(63.7%), <it>Vigna </it>(25.9%), <it>Glycine </it>(19.8%), <it>Medicago </it>(10.2%), <it>Dipterix </it>(6%) and <it>Arachis </it>(1.8%) genera. The average PIC (Polymorphism Information Content) varied from 0.53 for genomic SSRs to 0.47 for EST-SSRs, and the average number of alleles per locus was 4 and 3, respectively. Among the 315 newly tested SSRs in the BJ (BAT93 X Jalo EEP558) population, 24% (76) were polymorphic. The integration of these segregant loci into a framework map composed of 123 previously obtained SSR markers yielded a total of 199 segregant loci, of which 182 (91.5%) were mapped to 14 linkage groups, resulting in a map length of 1,157 cM.</p> <p>Conclusions</p> <p>A total of 302 newly developed EST-SSR markers, showing good amplification quality, are available for the genetic analysis of <it>Phaseolus vulgaris</it>. These markers showed satisfactory rates of transferability, especially between species that have great economic and genomic values. Their diversity was comparable to genomic SSRs, and they were incorporated in the common bean reference genetic map, which constitutes an important contribution to and advance in <it>Phaseolus vulgaris </it>genomic research.</p

Arroz preto: nova opção culinária para o Brasil.

A Embrapa Arroz e Feijão desenvolveu algumas linhagens de arroz preto com grão similar ao tipo agulhinha e realizou alguns estudos agronômicos e de qualidade de grãos com o objetivo de estimular a ampliação de novos mercados para os produtores atingirem um nicho específico, com potencial no consumo interno e externo, já que o arroz preto tem amplo mercado na Europa e nos Estados Unidos. Apesar de ainda incipiente no Brasil, é grande o potencial de mercado em qualquer região brasileira com vantagens nutricionais, funcionais e culinárias agregadas. O arroz preto deve ser considerado um produto especial e ser analisado como tal, não sendo o intuito do trabalho recomendar um tipo de arroz colorido para substituir o arroz branco ou integral na dieta brasileira, mas sim, sugerir alternativas de diversificação culinária e de geração de renda.bitstream/CNPAF-2009-09/27598/1/comt_147.pd

Characterization of oral enterobacteriaceae prevalence and resistance profile in chronic kidney disease patients undergoing peritoneal dialysis

Chronic Kidney Disease (CKD) is a growing public-health concern worldwide. Patients exhibit compromised immunity and are more prone to infection than other populations. Therefore, oral colonization by clinically relevant members of the Enterobacteriaceae family, major agents of both nosocomial and dialysis-associated infections with frequent prevalence of antibiotic resistances, may constitute a serious risk. Thus, this study aimed to assess the occurrence of clinically relevant enterobacteria and their antibiotic resistance profiles in the oral cavity of CKD patients undergoing peritoneal dialysis (CKD-PD) and compare it to healthy controls. Saliva samples from all the participants were cultured on MacConkey Agar and evaluated regarding the levels of urea, ammonia, and pH. Bacterial isolates were identified and characterized for antibiotic resistance phenotype and genotype. The results showed that CKD-PD patients exhibited significantly higher salivary pH, urea, and ammonia levels than controls, that was accompanied by higher prevalence and diversity of oral enterobacteria. Out of all the species isolated, only the prevalence of Raoultella ornithinolytica varied significantly between groups, colonizing the oral cavity of approximately 30% of CKD-PD patients while absent from controls. Antibiotic resistance phenotyping revealed mostly putative intrinsic resistance phenotypes (to amoxicillin, ticarcillin, and cephalothin), and resistance to sulfamethoxazole (~43% of isolates) and streptomycin (~17%). However, all isolates were resistant to at least one of the antibiotics tested and multidrug resistance isolates were only found in CKD-PD group (31,6%). Mobile genetic elements and resistance genes were detected in isolates of the species Raoultella ornithinolytica, Klebsiella pneumoniae, Klebsiella oxytoca, Escherichia coli, and Enterobacter asburiae, mostly originated from CKD-PD patients. PD-related infection history revealed that Enterobacteriaceae were responsible for ~8% of peritonitis and ~ 16% of exit-site infections episodes in CKD-PD patients, although no association was found to oral enterobacteria colonization at the time of sampling. The results suggest that the CKD-induced alterations of the oral milieu might promote a dysbiosis of the commensal oral microbiome, namely the proliferation of clinically relevant Enterobacteriaceae potentially harboring acquired antibiotic resistance genes. This study highlights the importance of the oral cavity as a reservoir for pathobionts and antibiotic resistances in CKD patients undergoing peritoneal dialysis.info:eu-repo/semantics/publishedVersio

Adaptabilidade e estabilidade de genótipos de soja na zona agreste dos estados da Bahia e Sergipe.

bitstream/CPATC-2010/21486/1/bp-49.pd

Atenção à saúde no Brasil: modelos de seguro social e seguridade social

O processo de organização do setor saúde no Brasil é marcado pelo contexto sócio, político e econômico que representa um período e dele, emerge determinado perfil epidemiológico e demográfico. Este estudo versa sobre o histórico da saúde pública no Brasil em um cenário onde passamos por distintos momentos, indo do seguro social à seguridade social, esta, como deflagradora do reconhecimento de cidadania ao povo brasileiro e com ela, o acesso à saúde. O objeto de estudo trata das características que traduzem a prática atual de assistência à saúde e sua veracidade comprovada na prática. O referido estudo mostrasse relevante uma vez que poderá suscitar a transformação das práticas de assistência à saúde ainda arraigadas em antigos modelos e que vem comprometendo a relação entre os cidadãos e os serviços. Desta forma, colaborando para uma utilização realmente universal, integral e com a devida equidade prevista pelo Sistema de Saúde e objetivando entender como a população compreende a saúde no Brasil. Entre outros objetivos, verificar o reconhecimento do SUS como um único sistema de saúde, identificar a qualidade de utilização dos seus serviços e propor maior disponibilização de informações sobre o SUS e sua potencial utilização. O SUS é um sistema com propostas de assistência que são norteadas por seus princípios e insere-se no atendimento à população brasileira com ações que rompem com os paradigmas da medicina curativa e ainda, trazendo para sua essência o novo modelo de seguridade social, participação popular e o controle social. Com isso o referido estudo poderá contribuir para o desenvolvimento de um usufruto democrático e participativo tanto na organização dos serviços de saúde como na sua aplicação. Trata-se de pesquisa bibliográfica, exploratória e descritiva com abordagem qualitativa

Measurement of Leptonic Asymmetries and Top Quark Polarization in ttbar Production

We present measurements of lepton (l) angular distributions in ttbar -> W+ b W- b -> l+ nu b l- nubar bbar decays produced in ppbar collisions at a center-of-mass energy of sqrt(s)=1.96TeV, where l is an electron or muon. Using data corresponding to an integrated luminosity of 5.4fb^-1, collected with the D0 detector at the Fermilab Collider, we find that the angular distributions of l- relative to anti-protons and l+ relative to protons are in agreement with each other. Combining the two distributions and correcting for detector acceptance we obtain the forward-backward asymmetry A^l_FB = (5.8 +- 5.1(stat) +- 1.3(syst))%, compared to the standard model prediction of A^l_FB (predicted) = (4.7 +- 0.1)%. This result is further combined with the measurement based on the analysis of the l+jets final state to obtain A^l_FB = (11.8 +- 3.2)%. Furthermore, we present a first study of the top-quark polarization.Comment: submitted versio

Search for B0→π0π0B^{0}\to \pi^{0}\pi^{0} Decay

We have searched for the charmless hadronic decay of B0 mesons into two neutral pions. Using 9.13fb^-1 taken at the Upsilon(4S) with the CLEO detector, we obtain an improved upper limit for the branching fraction BR(B0-->pi0pi0) < 5.7*10^-6 at the 90% confidence level.Comment: pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Search for Zgamma events with large missing transverse energy in ppbar collisions at sqrt(s)=1.96 TeV

We present the first search for supersymmetry (SUSY) in Zgamma final states with large missing transverse energy using data corresponding to an integrated luminosity of 6.2 fb-1 collected with the D0 experiment in ppbar collisions at sqrt(s)=1.96 TeV. This signature is predicted in gauge-mediated SUSY-breaking models, where the lightest neutralino is the next-to-lightest supersymmetric particle (NLSP) and is produced in pairs, possibly through decay from heavier supersymmetric particles. The NLSP can decay either to a Z boson or a photon and an associated gravitino that escapes detection. We exclude this model at the 95% C.L. for SUSY breaking scales of Lambda < 87 TeV, corresponding to neutralino masses of < 151 GeV.Comment: submitted to Phys. Rev. Let

Measurement of the semileptonic charge asymmetry using Bs^0 -> D_s mu X decays

We present a measurement of the time-integrated flavor-specific semileptonic charge asymmetry in the decays of Bs mesons that have undergone flavor mixing, a^s_ls, using B_s^0 (barB_s^0) -> D_s^-/+ mu^+/- X decays, with D_s^-/+ -> phi pi^-/+ and phi K^+ K^-, using 10.4 fb^-1 of proton-antiproton collisions collected by the D0 detector during Run II at the Fermilab Tevatron Collider. A fit to the difference between the time-integrated D_s^-$ and D_s^+ mass distributions of the B_s^0 and barB^0_s candidates yields the flavor-specific asymmetry a^s_ls = [-1.12 pm 0.74 (stat) +/-0.17 (syst)]% which is the most precise measurement and in agreement with the standard model prediction.Comment: 7 pages, 3 figures (updated with changes made in journal review process

Search for the Higgs boson in lepton, tau and jets final states

We present a search for the standard model Higgs boson in final states with an electron or muon and a hadronically decaying tau lepton in association with two or more jets using 9.7 fb^{-1} of Run II Fermilab Tevatron Collider data collected with the D0 detector. The analysis is sensitive to Higgs boson production via gluon fusion, associated vector boson production, and vector boson fusion, followed by the Higgs boson decay to tau lepton pairs or to W boson pairs. The ratios of 95% C.L. upper limits on the cross section times branching ratio to those predicted by the standard model are obtained for orthogonal subsamples that are enriched in either H -> tau tau decays or H -> WW decays, and for the combination of these subsample limits. The observed and expected limit ratios for the combined subsamples at a Higgs boson mass of 125 GeV are 11.3 and 9.0 respectively