Norms for 150 consumer products: Perceived complexity, quality objectivity, material/experiential nature, perceived price, familiarity and attitude

Consumer products are widely used as stimuli across several research fields. The use of consumer products as experimental stimuli lacks, however, the support of normative data regarding product features variability. In this work, we provide a first set of norms for people's perceptions of 150 consumer products regarding six relevant dimensions: product perceived complexity, quality objectivity, material/experiential nature, perceived price, familiarity and attitude. Products available in this normative database showed good overall distribution across the range of the dimensions evaluated. Obtained correlations between some of these dimensions provided evidence of how they can be confounded across products, further justifying the need to control for these dimensions. These norms should aid future research by allowing researchers to select products according to specific attributes and achieve appropriate experimental control. The norms here provided should also aid consumer behavior practitioners (such as marketers and advertisers) by providing insights as to how consumers perceive products along relevant dimensions.info:eu-repo/semantics/publishedVersio

Phage Therapy of Mycobacterium Infections: Compassionate Use of Phages in 20 Patients With Drug-Resistant Mycobacterial Disease

Mycobacteriophage; Nontuberculous mycobacteria; Phage therapyMicobacteriófago; Micobacterias no tuberculosas; Terapia de fagosMicobacteriòfag; Micobacteris no tuberculosos; Teràpia de fagsBackground Nontuberculous Mycobacterium infections, particularly Mycobacterium abscessus, are increasingly common among patients with cystic fibrosis and chronic bronchiectatic lung diseases. Treatment is challenging due to intrinsic antibiotic resistance. Bacteriophage therapy represents a potentially novel approach. Relatively few active lytic phages are available and there is great variation in phage susceptibilities among M. abscessus isolates, requiring personalized phage identification. Methods Mycobacterium isolates from 200 culture-positive patients with symptomatic disease were screened for phage susceptibilities. One or more lytic phages were identified for 55 isolates. Phages were administered intravenously, by aerosolization, or both to 20 patients on a compassionate use basis and patients were monitored for adverse reactions, clinical and microbiologic responses, the emergence of phage resistance, and phage neutralization in serum, sputum, or bronchoalveolar lavage fluid. Results No adverse reactions attributed to therapy were seen in any patient regardless of the pathogen, phages administered, or the route of delivery. Favorable clinical or microbiological responses were observed in 11 patients. Neutralizing antibodies were identified in serum after initiation of phage delivery intravenously in 8 patients, potentially contributing to lack of treatment response in 4 cases, but were not consistently associated with unfavorable responses in others. Eleven patients were treated with only a single phage, and no phage resistance was observed in any of these. Conclusions Phage treatment of Mycobacterium infections is challenging due to the limited repertoire of therapeutically useful phages, but favorable clinical outcomes in patients lacking any other treatment options support continued development of adjunctive phage therapy for some mycobacterial infections

Influence of Fitness on Stress Reactivity as Measured with the Trier Social Stress Test

PURPOSE: Psychosocial stress is associated with multiple health complaints which a higher cardiorespiratory fitness may reduce stress reactivity. A higher level of fitness may assist in the reduction of stress-related risk factors. Limited studies have investigated the health and cortisol response of promotoras’ (Latina community health educators) physical activity (PA) behaviors along the South Texas Mexico Border Region. The aim of this study was to assess the associations of promotoras’ fitness on stress reactivity in promotoras. METHODS: 17 promotoras’ anthropometric measures (body mass index (BMI)), were assessed before performing Trier Social Stress Test (TSST). Salivary cortisol was collected before, 10-minutes, 25-minutes, and 40-minutes post TSST via passive drool method. Self-report PA included Jurca non-exercise assessment of cardiorespiratory capacity. Promotoras also performed a 2-minute step test. All tests were assessed using Spearman correlation analyses at a significance level of .05. RESULTS: Participants’ average BMI was high (31.4±7.18 kg/m²); 76.5% were overweight or obese, 30% very or extremely obese. Physical functioning levels were low (cardiorespiratory capacity 26.0±9.1 VO2/kg/min; step test 77.4 steps/2-minutes). Salivary cortisol levels revealed significant increase at the onset of the TSST and remained significantly elevated at 10-minutes post TSST. In comparison to baseline, cortisol levels remained elevated at 25-minutes and 40-minutes post TSST. CONCLUSION: Low levels of cardiorespiratory fitness and high BMI’s may have resulted in a slower cortisol recovery time after placing the body under stress in promotoras. Regular physical activity and fitness may attenuate response to psychosocial stress and enable promotoras to achieve a healthier lifestyle

Physical Fitness and Energy Balance Activity of “Promotora” Community Health Care Workers in the South Texas-Mexico Border Region

PURPOSE: Latinas along the U.S.-Mexico border are among the most physically inactive and obese segment of the U.S. population. There is widespread recognition of the cultural appropriateness of promotoras (community health workers) in health education among Latino communities. METHODS: We conducted a cross-sectional study with 17 promotoras who reside/work in the region to investigate their physical fitness and energy balance behaviors. Promotoras completed physical testing and self-report on physical activity and dietary behaviors. Participants wore an accelerometer for one week. In addition, the validity of an activity self-report instrument to assess cardiorespiratory fitness was tested. RESULTS: Participants’ average body mass index (BMI) was high (31.4±7.18 kg/m²); 76.5% were overweight or obese, 30% very or extremely obese. Physical functioning levels were low (cardiorespiratory capacity 26.0±9.1 VO2/kg/min; step test 77.4 steps/2-minutes). Objectively measured moderate-vigorous PA (114.6 ± 87.0 minutes/week) was less than PA recommendations. Accelerometry activity significantly correlated with self-report (r = 0.71, p=0.009). Participants self-reported consuming 1889.2±492.8 kCal/day. CONCLUSION: Promotoras’ energy balance behaviors were similar to women where they reside/work. Promotoras engaging in health-promoting behaviors may enhance their effectiveness as change agents in the communities they serve

Vector Competence of American Mosquitoes for Three Strains of Zika Virus

In 2015, Zika virus (ZIKV; Flaviviridae; Flavivirus) emerged in the Americas, causing millions of infections in dozens of countries. The rapid spread of the virus and the association with disease outcomes such as Guillain-Barré syndrome and microcephaly make understanding transmission dynamics essential. Currently, there are no reports of vector competence (VC) of American mosquitoes for ZIKV isolates from the Americas. Further, it is not clear whether ZIKV strains from other genetic lineages can be transmitted by American Aedes aegypti populations, and whether the scope of the current epidemic is in part facilitated by viral factors such as enhanced replicative fitness or increased vector competence. Therefore, we characterized replication of three ZIKV strains, one from each of the three phylogenetic clades in several cell lines and assessed their abilities to be transmitted by Ae. aegypti mosquitoes. Additionally, laboratory colonies of different Culex spp. were infected with an American outbreak strain of ZIKV to assess VC. Replication rates were variable and depended on virus strain, cell line and MOI. African strains used in this study outcompeted the American strain in vitro in both mammalian and mosquito cell culture. West and East African strains of ZIKV tested here were more efficiently transmitted by Ae. aegypti from Mexico than was the currently circulating American strain of the Asian lineage. Long-established laboratory colonies of Culex mosquitoes were not efficient ZIKV vectors. These data demonstrate the capacity for additional ZIKV strains to infect and replicate in American Aedes mosquitoes and suggest that neither enhanced virus replicative fitness nor virus adaptation to local vector mosquitoes seems likely to explain the extent and intensity of ZIKV transmission in the Americas

Smell of Infection:a novel, non-invasive method for detection of fish excretory- secretory proteins

Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding, and immune response stimulated upon infection. Alarm cues are often released in the form of proteins, antimicrobial peptides, and immunoglobulins that provide important insights into bodily function and infection status. Here we outline a noninvasive method to identify potential chemical cues associated with infection in fish by extracting, purifying, and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that teleost proteins can be characterized from water and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a noninvasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied ecology and aquatic biology

Urban narrative: Value based urban design

Urban Narrative focuses on the social dimension within urban design which is often overlooked in practice which examines human values and needs as a means to inform design practice. The project has been working in partnership with two councils in New Zealand in relation to the social dimension

Stress and worry in the 2020 coronavirus pandemic: relationships to trust and compliance with preventive measures across 48 countries in the COVIDiSTRESS global survey

The COVIDiSTRESS global survey collects data on early human responses to the 2020 COVID-19 pandemic from 173 429 respondents in 48 countries. The open science study was co-designed by an international consortium of researchers to investigate how psychological responses differ across countries and cultures, and how this has impacted behaviour, coping and trust in government efforts to slow the spread of the virus. Starting in March 2020, COVIDiSTRESS leveraged the convenience of unpaid online recruitment to generate public data. The objective of the present analysis is to understand relationships between psychological responses in the early months of global coronavirus restrictions and help understand how different government measures succeed or fail in changing public behaviour. There were variations between and within countries. Although Western Europeans registered as more concerned over COVID-19, more stressed, and having slightly more trust in the governments' efforts, there was no clear geographical pattern in compliance with behavioural measures. Detailed plots illustrating between-countries differences are provided. Using both traditional and Bayesian analyses, we found that individuals who worried about getting sick worked harder to protect themselves and others. However, concern about the coronavirus itself did not account for all of the variances in experienced stress during the early months of COVID-19 restrictions. More alarmingly, such stress was associated with less compliance. Further, those most concerned over the coronavirus trusted in government measures primarily where policies were strict. While concern over a disease is a source of mental distress, other factors including strictness of protective measures, social support and personal lockdown conditions must also be taken into consideration to fully appreciate the psychological impact of COVID-19 and to understand why some people fail to follow behavioural guidelines intended to protect themselves and others from infection. The Stage 1 manuscript associated with this submission received in-principle acceptance (IPA) on 18 May 2020. Following IPA, the accepted Stage 1 version of the manuscript was preregistered on the Open Science Framework at https://osf.io/ytbcs. This preregistration was performed prior to data analysis

Metabolic Activation of Benzo[a]pyrene by Human Tissue Organoid Cultures

Organoids are 3D cultures that to some extent reproduce the structure, composition and function of the mammalian tissues from which they derive, thereby creating in vitro systems with more in vivo-like characteristics than 2D monocultures. Here, the ability of human organoids derived from normal gastric, pancreas, liver, colon and kidney tissues to metabolise the environmental carcinogen benzo[a]pyrene (BaP) was investigated. While organoids from the different tissues showed varied cytotoxic responses to BaP, with gastric and colon organoids being the most susceptible, the xenobiotic-metabolising enzyme (XME) genes, CYP1A1 and NQO1, were highly upregulated in all organoid types, with kidney organoids having the highest levels. Furthermore, the presence of two key metabolites, BaP-t-7,8-dihydrodiol and BaP-tetrol-l-1, was detected in all organoid types, confirming their ability to metabolise BaP. BaP bioactivation was confirmed both by the activation of the DNA damage response pathway (induction of p-p53, pCHK2, p21 and γ-H2AX) and by DNA adduct formation. Overall, pancreatic and undifferentiated liver organoids formed the highest levels of DNA adducts. Colon organoids had the lowest responses in DNA adduct and metabolite formation, as well as XME expression. Additionally, high-throughput RT-qPCR explored differences in gene expression between organoid types after BaP treatment. The results demonstrate the potential usefulness of organoids for studying environmental carcinogenesis and genetic toxicology

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy