Legal framework and scientific responsibilities during volcanic crises: the case of the El Hierro eruption (2011–2014)

In recent years concerns have been growing in the scientific community over the definition of scientific responsibilities during emergencies, and the legal status of scientists involved in the corresponding decision-making. It is clear that the legal framework is one of the main elements affecting this issue; however, many factors may affect both the specific scientific decision-making and the definition of general scientific responsibilities. The situation will vary depending on the type and scale of emergency, and from place to place, even in the same country. There will be no such thing as a single, ideal solution. In the latest El Hierro volcanic crisis many factors have negatively affected the scientific management and have prevented an adequate definition of scientific responsibility. These factors have been detected and documented by the authors. They include excessive pressure due to human and economic issues, a poor legal framework with identifiable deficiencies, an Emergency Plan in which the Volcanic Activity/Alert Level (VAL), Emergency Response Level (ERL) and Volcanic Traffic Light (VTL) have been too rigidly linked, serious weaknesses in the management and structure of the Scientific Committee (SC), and more. Even though some of these problems have now been detected and certain solutions have already been proposed, the slowness and complexity of the bureaucratic processes are making it difficult to implement solutions.This research has been funded by the CSIC (201130E070) and MINECO (CGL2011-28682- C02-01) research projects.Peer reviewe

Reconstructing palaeo-volcanic geometries using a Geodynamic Regression Model (GRM): Application to Deception Island volcano (South Shetland Islands, Antarctica)

This article describes a reconstruction made of the palaeo-volcanic edifice on Deception Island (South Shetland Islands, Antarctica) prior to the formation of its present caldera. Deception Island is an active Quaternary volcano located in the Bransfield Strait, between the South Shetland Islands and the Antarctic Peninsula. The morphology of the island has been influenced mainly by the volcanic activity but geodynamics and volcanic deformation have also contributed. A volcanic reconstruction method, the Geodynamic Regression Model (GRM), which includes a terrain deformation factor, is proposed. In the case of Deception Island, the directions of this deformation are NW–SE and NE–SW, and match both the observed deformation of the Bransfield Strait and the volcanic deformation monitored over the last 20 years in the island, using Global Navigation Satellite System (GNSS) techniques. Based on these data, possible volcanic deformation values of 5–15 mm/yr in these directions have been derived. A possible coastline derived from a current bathymetry is transformed, according to values for the chosen date, to obtain the palaeo-coastline of Deception Island of 100 k years ago. Topographic, geomorphologic, volcanological and geological data in a GIS system have been considered, for computation of the outside caldera slope, palaeo-coastline, palaeo-summit height and palaeo digital elevation model (DEM). The result is a 3D palaeo-geomorphological surface model of a volcano, reaching 640 m in height, with an increase of 4 km3 in volume compared to the current edifice, covering 4 km2 more surface area and the method reveals the previous existence of parasite volcanoes. Two photorealistic images of the island are obtained by superposition of textures extracted from a current Quick Bird satellite image also. This technique for reconstructing the terrain of an existing volcano could be useful for analysing the past and future geomorphology of this island and similar locations

Normal vector analysis from GNSS-GPS data applied to Deception volcano surface deformation

Surface deformation parameters and its use in volcano monitoring have evolved from classical geodetic procedures up to those based on Global Navigation Satellite Systems (GNSS), in particular the most widely used and known Global Positioning System (GPS), profiting from the automated data processing, positioning precision and rates, as well as the large storage capacity and low power consumption of its equipments. These features have enabled the permanent GNSSGPS data acquisition to ensure the continuous monitoring of geodetic benchmarks for the evaluation of surface deformation in active tectonic or volcanic areas. In Deception Island (Antarctica), a normal vector analysis is being used to give surface deformation based on three permanently observed GNSSGPS benchmarks. Due to data availability, both in the past and for near real-time use, all benchmarks used are inside the monitored volcanic area, although the reference is away from thermal springs and/or fumaroles, unlike the other two. The time variation of slope distances to the reference benchmark and of the magnitude and inclination of the normal vector to the triangle defined by the reference benchmark and any other two, provides the spatial deformation in the volcanic area covered. The normal vector variation in magnitude gives information on compression or expansion, here called spatial dilatometer, while the changes in inclination gives information on relative uplift or subsidence, here called spatial inclinometer. In geodesy, the triangle is a basic geometric unit and the areal strain is commonly applied in tectonics and volcanism. The normal vector analysis conjugates both, benefiting from the method's precision, simplicity and possibility to model the surface using several triangles. The proposed method was applied to GNSSGPS data collected every austral summer between 20012002 and 20092010 in Deception Island. The results evidence that Deception Island acts as a strain marker in the Bransfield Basin volcano-tectonic setting.Spanish Ministry of Education and Science as part of the National Antarctic Program; Recognition and fast evaluation of volcanic activity on Deception Island (GEODESY) [ANT1999-1430-E/HESP]; Geodetic Studies on Deception Island: deformation models, geoid determination and Scientific Information System [REN2000-0551-C03-01/ANT]; Acquisition of scientific software for GPS data processing [REN2000-2690-E]; Geodetic Control of the volcanic activity of Deception Island [CGL2004-21547-E/ANT]; Update of the Spanish Cartography for Deception Island [CGL2004-20408-E/ANT]; Volcanotectonic activity on Deception Island: geodetic, geophysical investigations and Remote Sensing on Deception Island and its surroundings [CGLl2005-07589-c03-01/ANT]; Geodetic and Geothermal Researches, Time Serial Analysis and Volcanic Innovation in Antarctica (South Shetland Islands and Antarctic Peninsula (GEOTINANT) [CTM2009-07251/ANT]info:eu-repo/semantics/publishedVersio

Effects of fiber inclusion on growth performance and nutrient digestibility of piglets reared under optimal or poor hygienic conditions

Two experiments were conducted to estimate the standardized ileal digestible (SID) Trp:Lys ratio requirement for growth performance of nursery pigs. Experimental diets were formulated to ensure that lysine was the second limiting AA throughout the experiments. In Exp. 1 (6 to 10 kg BW), 255 nursery pigs (PIC 327 × 1050, initially 6.3 ± 0.15 kg, mean ± SD) arranged in pens of 6 or 7 pigs were blocked by pen weight and assigned to experimental diets (7 pens/diet) consisting of SID Trp:Lys ratios of 14.7%, 16.5%, 18.4%, 20.3%, 22.1%, and 24.0% for 14 d with 1.30% SID Lys. In Exp. 2 (11 to 20 kg BW), 1,088 pigs (PIC 337 × 1050, initially 11.2 kg ± 1.35 BW, mean ± SD) arranged in pens of 24 to 27 pigs were blocked by average pig weight and assigned to experimental diets (6 pens/diet) consisting of SID Trp:Lys ratios of 14.5%, 16.5%, 18.0%, 19.5%, 21.0%, 22.5%, and 24.5% for 21 d with 30% dried distillers grains with solubles and 0.97% SID Lys. Each experiment was analyzed using general linear mixed models with heterogeneous residual variances. Competing heteroskedastic models included broken-line linear (BLL), broken-line quadratic (BLQ), and quadratic polynomial (QP). For each response, the best-fitting model was selected using Bayesian information criterion. In Exp. 1 (6 to 10 kg BW), increasing SID Trp:Lys ratio linearly increased (P 24.0%]) SID Trp:Lys ratio. For G:F, the best-fitting model was a BLL in which the maximum G:F was estimated at 20.4% (95% CI: [14.3%, 26.5%]) SID Trp:Lys. In Exp. 2 (11 to 20 kg BW), increasing SID Trp:Lys ratio increased (P < 0.05) ADG and G:F in a quadratic manner. For ADG, the best-fitting model was a QP in which the maximum ADG was estimated at 21.2% (95% CI: [20.5%, 21.9%]) SID Trp:Lys. For G:F, BLL and BLQ models had comparable fit and estimated SID Trp:Lys requirements at 16.6% (95% CI: [16.0%, 17.3%]) and 17.1% (95% CI: [16.6%, 17.7%]), respectively. In conclusion, the estimated SID Trp:Lys requirement in Exp. 1 ranged from 20.4% for maximum G:F to 23.9% for maximum ADG, whereas in Exp. 2 it ranged from 16.6% for maximum G:F to 21.2% for maximum ADG. These results suggest that standard NRC (2012) recommendations may underestimate the SID Trp:Lys requirement for nursery pigs from 11 to 20 kg BW

The role of the noradrenergic system in the affective sphere of chronic neuropathic pain

Chronic pain is a complex experience comprising two different components: sensorial and affective components of pain. Chronic pain can become maladaptive and incapacitating leading to a worsening in the prognosis, the response to treatment and reducing life quality of patients. Furthermore, many psychobiological factors get worse the affective component of pain leading to depressive symptoms. However, little is known about the possible modifications of pain processing when chronic pain condition manifests symptoms of depression. Noradrenergic system is a pivotal candidate projecting descending and ascending regulating sensorial and emotional aspects of pain, respectively. In the present study, we assessed the role of noradrenergic system in the sensorial and emotional components of pain in rats submitted to chronic pain and/or depression. Chronic constriction injury (CCI)[1] was used as a model of chronic neuropathic pain and chronic mild stress (CMS)[2] as a model of depression, generating four experimental groups: Sham-control, Sham-CMS, CCIcontrol and CCI-CMS. Parallel, in order to study the involvement of noradrenergic system in the sensorial and affective components of pain, DSP-4 (50mg/Kg), a neurotoxin which selectively damages noradrenergic projections and Desipramine (DMI, (10mg/Kg), a noradrenaline reuptake inhibitor, were administered intraperitoneally to CCI-control and CCI-CMS groups the same day of CCI and CMS induction. After 14 days of treatment, sensorial and affective components of pain were evaluated by using nociceptive tests (von Frey and acetone test) as well as the place escape avoidance test (PEAT)[3], respectively. Additionally, we evaluated anhedonia in all experimental groups. All results were analyzed using two-way analysis of variance (ANOVA) with or without repeated measures, as appropriate. The results revealed that the group with chronic pain and depression (CCI-CMS) showed the most negative pain experience in the PEAT followed by the group with chronic pain (CCI-control). However, DMI administration prevented the worsening of affective component of pain experimented by CCI-control and CCI-CMS groups. Interestingly, DSP-4 administration enhancement the negative pain experience in CCI-control group showing similar score that CCI-CMS in the PEAT. Regarding to the sensory component of pain, both CCI-control and CCI-CMS groups showing similar degree of mechanical allodynia in the von Frey test. DMI administration prevented the development of allodynia while DSP-4 did not show significant changes in nociceptive threshold. On the other hand, both Sham-CMS and CCI-CMS development anhedonia after 14 days of CMS and DMI prevented it. Interestingly, DSP-4 administration induced anhedonia in CCI-control and enhancement anhedonia in CCI-CMS group. In conclusion, depression highly determines affective-pain experience and the inhibition of noradrenaline reuptake prevents it. On the other hand, the destruction of noradrenergic system, by DSP-4 administration, enhancement the negative pain experience leading to a concomitant state of chronic pain and depression. Overall suggests that noradrenergic system play a major role regulating the affective-interpretative pain experience

Cardioembolic Stroke Diagnosis Using Blood Biomarkers

Stroke is one of the main causes of death and disability in the world. Cardioembolic etiology accounts for approximately one fifth of all ischemic strokes whereas 25-30% remains undetermined even after an advanced diagnostic workup. Despite there is not any biomarker currently approved to distinguish cardioembolic stroke among other etiologies in clinical practice the use of biomarkers represents a promising valuable complement to determine stroke etiology reducing the number of cryptogenic strokes and aiding in the prescription of the most appropriated primary and secondary treatments in order to minimize therapeutic risks and to avoid recurrences. In this review we present an update about specific cardioembolic stroke-related biomarkers at a protein, transcriptomic and genetic level. Finally, we also focused on reported biomarkers associated with atrial fibrillation (a cardiac illness strongly related with cardioembolic stroke subtype) thus with a potential to become biomarkers to detect cardioembolic stroke in the future

Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy

Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 mu m) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions

Identificación y uso de biomarcadores pronósticos en el ictus isquémico

II. Es van analitzar mostres de microdiàlisi cerebral in vivo de pacients d'ictus isquèmic pel descobriment de biomarcadors associats a la malaltia cerebrovascular. Es van obtenir microdialitzats (MDs) de la zona infartada, de la zona del periinfart i de la zona contralateral no afectada. Els MDs es van comparar, qualitativament i quantitativament, mitjançant tècniques de proteómica basades en marcatge isobàric i espectrometria de masses. Complementàriament, alguns candidats es van estudiar mitjançant immunotransferència en mostres de teixit de necròpsies cerebrals de pacients morts a causa d'un ictus isquèmic. Finalment, hem començat a realitzar ELISAs per la determinació d'aquests candidats en mostres de sang.I. Se analizaron muestras de microdiálisis cerebral in vivo de pacientes de ictus isquémico para el descubrimiento de biomarcadores asociados a la enfermedad cerebrovascular. Se obtuvieron microdializados (MDs) de la zona infartada, de la zona del periinfarto y de la zona contralateral no afectada. Los MDs fueron comparados, cualitativa y cuantitativamente, mediante técnicas de proteómica basadas en marcaje isobárico y espectrometría de masas. Complementariamente, algunos candidatos se estudiaron mediante inmunotransferencia en muestras de tejido de necropsias cerebrales de pacientes fallecidos a causa de un ictus isquémico. Finalmente, hemos comenzado a realizar ELISAs para la determinación de estos candidatos en muestras de sangre

Chemogenetic silencing of the locus coeruleus–basolateral amygdala pathway abolishes pain-induced anxiety and enhanced aversive learning in rats.

BACKGROUND: Pain affects both sensory and emotional aversive responses, often provoking anxiety-related diseases when chronic. However, the neural mechanisms underlying the interactions between anxiety and chronic pain remain unclear. METHODS: We characterized the sensory, emotional, and cognitive consequences of neuropathic pain (chronic constriction injury) in a rat model. Moreover, we determined the role of the locus coeruleus (LC) neurons that project to the basolateral amygdala (BLA) using a DREADD (designer receptor exclusively activated by designer drugs). RESULTS: Chronic constriction injury led to sensorial hypersensitivity in both the short term and long term. Otherwise, long-term pain led to an anxiety-like profile (in the elevated zero maze and open field tests), as well as increased responses to learn aversive situations (in the passive avoidance and fear conditioning tests) and an impairment of non-emotional cognitive tasks (in the novel object recognition and object pattern of separation tests). Chemogenetic blockade of the LC-BLA pathway and intra-BLA or systemic antagonism of beta-adrenergic receptors abolished both long-term pain-induced anxiety and enhanced fear learning. By contrast, chemogenetic activation of this pathway induced anxiety-like behaviors and enhanced the aversive learning and memory index in sham animals, although it had little effect on short- and long-term chronic constriction injury animals. Interestingly, modulation of LC-BLA activity did not modify sensorial perception or episodic memory. CONCLUSIONS: Our results indicate that dimensions associated with pain are processed by independent pathways and that there is an overactivation of the LC-BLA pathway when anxiety and chronic pain are comorbid, which involves the activity of beta-adrenergic receptors

Depressive-like states heighten the aversion to painful stimuli in a rat model of comorbid chronic pain and depression.

BACKGROUND: Chronic pain and depression are two complex states with sensory/somatic and emotional components, and they may mutually exacerbate one another in conditions of comorbidity, leading to a poorer prognosis. METHODS: The authors have evaluated the sensory and emotional components in a rat model combining chronic constriction injury (CCI, a model of chronic neuropathic pain) with unpredictable chronic mild stress (CMS, an experimental model of depression). In addition, the phosphorylation/activation of the extracellular signal-regulated kinases 1 and 2 and neuronal density was also evaluated in the anterior cingulate cortex. Four groups were tested: sham-control, sham-CMS, CCI-control, and CCI-CMS. RESULTS: CMS selectively heightens aversion to painful experiences in animals subjected to CCI, as measured in the place escape/avoidance test at 20, 25, and 30 min (CCI-CMS (mean±SEM): 75.68±3.32, 66.75±4.70, 77.54±3.60 vs. CCI-control: 44.66±6.07, 43.17±6.92, 52.83±5.92, respectively), in conjunction with an increase in the accumulation of phosphorylation/activation of the extracellular signal-regulated kinases (CCI-CMS: 4.17±0.52 vs. sham-control: 0.96±0.05) and a decrease in neuronal density in the anterior cingulate cortex. In contrast, chronic pain did not exacerbate the characteristic profile of depression (anhedonia and behavioral despair) in rats subjected to CMS. Furthermore, depression enhances the perception of some specific modalities of sensorial pain such as cold allodynia but has no influence on mechanical threshold. CONCLUSIONS: These findings support the theory that depression leads to emotional dysfunction in the interpretation of pain in patients suffering chronic pain. In addition, combined animal models of pain-depression may provide a valuable tool to study the comorbidity of pain and depression