Carbon tetrachloride induced kidney and lung tissue damages and antioxidant activities of the aqueous rhizome extract of Podophyllum hexandrum

BACKGROUND: The present study was conducted to evaluate the in vitro and in vivo antioxidant properties of aqueous extract of Podophyllum hexandrum. The antioxidant potential of the plant extract under in vitro situations was evaluated by using two separate methods, inhibition of superoxide radical and hydrogen peroxide radical. Carbon tetrachloride (CCl(4)) is a well known toxicant and exposure to this chemical is known to induce oxidative stress and causes tissue damage by the formation of free radicals. METHODS: 36 albino rats were divided into six groups of 6 animals each, all animals were allowed food and water ad libitum. Group I (control) was given olive oil, while the rest groups were injected intraperitoneally with a single dose of CCl(4 )(1 ml/kg) as a 50% (v/v) solution in olive oil. Group II received CCl(4 )only. Group III animals received vitamin E at a concentration of 50 mg/kg body weight and animals of groups IV, V and VI were given extract of Podophyllum hexandrum at concentration dose of 20, 30 and 50 mg/kg body weight. Antioxidant status in both kidney and lung tissues were estimated by determining the activities of antioxidative enzymes, glutathione reductase (GR), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and superoxide dismutase (SOD); as well as by determining the levels of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS). In addition, superoxide and hydrogen peroxide radical scavenging activity of the extract was also determined. RESULTS: Results showed that the extract possessed strong superoxide and hydrogen peroxide radical scavenging activity comparable to that of known antioxidant butylated hydroxy toluene (BHT). Our results also showed that CCl(4 )caused a marked increase in TBARS levels whereas GSH, SOD, GR, GPX and GST levels were decreased in kidney and lung tissue homogenates of CCl(4 )treated rats. Aqueous extract of Podophyllum hexandrum successfully prevented the alterations of these effects in the experimental animals. CONCLUSION: Our study demonstrated that the aqueous extract of Podophyllum hexandrum could protect the kidney and lung tissue against CCl(4 )induced oxidative stress probably by increasing antioxidant defense activities

Search for time-dependent CPCP violation in D0→π+π−π0D^0 \rightarrow \pi^+ \pi^- \pi^0 decays

International audienceA measurement of time-dependent $CP$ violation in $D^0 \rightarrow \pi^+ \pi^- \pi^0$ decays using a $pp$ collision data sample collected by the LHCb experiment in 2012 and from 2015 to 2018, corresponding to an integrated luminosity of 7.7$\,\mathrm{fb}^{-1}$, is presented. The initial flavour of each $D^0$ candidate is determined from the charge of the pion produced in the $D^*(2010)^+ \rightarrow D^0 \pi^+$ decay. The decay $D^0 \rightarrow K^- \pi^+ \pi^0$ is used as a control channel to validate the measurement procedure. The gradient of the time-dependent $CP$ asymmetry, $\Delta Y$, in $D^0 \rightarrow \pi^+ \pi^- \pi^0$ decays is measured to be \begin{equation*} \Delta Y = (-1.3 \pm 6.3 \pm 2.4) \times 10^{-4}, \end{equation*} where the first uncertainty is statistical and the second is systematic, which is compatible with $CP$ conservation

Amplitude analysis and branching fraction measurement of B+→D∗−Ds+π+B^{+}\to D^{*-}D^{+}_{s}\pi^{+} decays

International audienceThe decays of the $B^{+}$ meson to the final state $D^{*-}D^{+}_{s}\pi^{+}$ are studied in proton-proton collision data collected with the LHCb detector at centre-of-mass energies of 7, 8, and 13 TeV, corresponding to a total integrated luminosity of 9 fb$^{-1}$. The ratio of branching fractions of the $B^{+}\to D^{*-}D^{+}_{s}\pi^{+}$ and $B^{0}\to D^{*-}D^{+}_{s}$ decays is measured to be $0.173\pm 0.006\pm 0.010$, where the first uncertainty is statistical and the second is systematic. Using partially reconstructed $D^{*+}_{s}\to D^{+}_{s}\gamma$ and $D^{+}_{s}\pi^{0}$ decays, the ratio of branching fractions between the $B^{+}\to D^{*-}D^{*+}_{s}\pi^{+}$ and $B^{+}\to D^{*-}D^{+}_{s}\pi^{+}$ decays is determined as $1.31\pm 0.07\pm 0.14$. An amplitude analysis of the $B^{+}\to D^{*-}D^{+}_{s}\pi^{+}$ decay is performed for the first time, revealing dominant contributions from known excited charm resonances decaying to the $D^{*-}\pi^{+}$ final state. No significant evidence of exotic contributions in the $D^{+}_{s}\pi^{+}$ or $D^{*-}D^{+}_{s}$ channels is found. The fit fraction of the scalar state $T_{c\bar{s} 0}^{\ast}(2900)^{++}$ observed in the $B^{+}\to D^{-}D^{+}_{s}\pi^{+}$ decay is determined to be less than 2.3% at a 90% confidence level

Tracking of charged particles with nanosecond lifetimes at LHCb

A method is presented to reconstruct charged particles with lifetimes between 10 ps and 10 ns, which considers a combination of their decay products and the partial tracks created by the initial charged particle. Using the $\Xi^-$ baryon as a benchmark, the method is demonstrated with simulated events and proton-proton collision data at $\sqrt{s}=13$ TeV, corresponding to an integrated luminosity of 2.0 fb${}^{-1}$ collected with the LHCb detector in 2018. Significant improvements in the angular resolution and the signal purity are obtained. The method is implemented as part of the LHCb Run 3 event trigger in a set of requirements to select detached hyperons. This is the first demonstration of the applicability of this approach at the LHC, and the first to show its scaling with instantaneous luminosity