Latest Cretaceous, Paleocene and Eocene floras from Patagonia: a critical piece of Gondwana's floral history comes into focus

Patagonia contains a rich and fairly continuous paleobotanical record from the late Maastrichtian to the middle Eocene, an interval known globally for mass extinction and significant climate changes. From this time interval, we have quantitatively collected and stratigraphically studied over 600 species and 20000 specimens from eight wellpreserved compression macrofloras from north-west and central Patagonia. Cretaceous and early Paleocene floras are from coastal environments, whereas the Eocene floras are from volcaniclastic lake sediments. Several sites and stratigraphic levels in the early Paleocene Salamanca Formation show higher richness than comparable Northern Hemisphere floras, but they are relatively impoverished when compared to those of the upper Maastrichtian Lefipan Formation. Probable middle Paleocene floras from the Peñas Coloradas Formation share most species with the Salamanca Formation, but are preserved in fluvial facies. Bedded tuffs at the Eocene sites have yielded precise Ar- Ar dates of 54.24 ±0.45 Ma for the Pampa de Jones flora, 51.91 ±0.22 Ma for the extremely diverse Laguna del Hunco flora, and 47.46 ±0.05 Ma for the similarly rich Río Pichileufú flora. The history of Patagonian plant diversity in the early Paleogene could be linked to a relatively lower effect of the terminal Cretaceous event and high diversification and immigration during Eocene warming. Although the biogeographic signature of the floras is clearly Gondwanic, during the Paleocene there appears to be greater compositional distinctiveness from coeval Australasian floras than in the Eocene, suggesting some degree of endemism after the K-T. The Eocene floras include beautifully preserved examples of extant Australasian tree genera that are now extinct in South America, including Agathis, Papuacedrus, Dacrycarpus, Eucalyptus, Cassuarinaeceae, and Akaniaceae. Paleoclimate analyses based on the macrofloras indicate subtropical and moist growing conditions; during the Eocene there is evidence for true rainforest conditions. These results contribute a considerably improved framework to understanding the plant evolution in Gondwana.Sesiones libresFinanciado: NSF Grants DEB-0919071 and DEB-0345750Financing: NSF Grants DEB-0919071 and DEB-0345750Financiamento: NSF Grants DEB-0919071 and DEB-0345750Facultad de Ciencias Naturales y Muse

Diagnostic aids in the screening of oral cancer

The World Health Organization has clearly indentified prevention and early detection as major objectives in the control of the oral cancer burden worldwide. At the present time, screening of oral cancer and its pre-invasive intra-epithelial stages, as well as its early detection, is still largely based on visual examination of the mouth. There is strong available evidence to suggest that visual inspection of the oral mucosa is effective in reducing mortality from oral cancer in individuals exposed to risk factors. Simple visual examination, however, is well known to be limited by subjective interpretation and by the potential, albeit rare, occurrence of dysplasia and early OSCC within areas of normal-looking oral mucosa. As a consequence, adjunctive techniques have been suggested to increase our ability to differentiate between benign abnormalities and dysplastic/malignant changes as well as to identify areas of dysplasia/early OSCC that are not visible to naked eye. These include the use of toluidine blue, brush biopsy, chemiluminescence and tissue autofluorescence. The present paper reviews the evidence supporting the efficacy of the aforementioned techniques in improving the identification of dysplastic/malignant changes of the oral mucosa. We conclude that available studies have shown promising results, but strong evidence to support the use of oral cancer diagnostic aids is still lacking. Further research with clear objectives, well-defined population cohorts, and sound methodology is strongly required

Bacterial Leaf Symbiosis in Angiosperms: Host Specificity without Co-Speciation

Bacterial leaf symbiosis is a unique and intimate interaction between bacteria and flowering plants, in which endosymbionts are organized in specialized leaf structures. Previously, bacterial leaf symbiosis has been described as a cyclic and obligate interaction in which the endosymbionts are vertically transmitted between plant generations and lack autonomous growth. Theoretically this allows for co-speciation between leaf nodulated plants and their endosymbionts. We sequenced the nodulated Burkholderia endosymbionts of 54 plant species from known leaf nodulated angiosperm genera, i.e. Ardisia, Pavetta, Psychotria and Sericanthe. Phylogenetic reconstruction of bacterial leaf symbionts and closely related free-living bacteria indicates the occurrence of multiple horizontal transfers of bacteria from the environment to leaf nodulated plant species. This rejects the hypothesis of a long co-speciation process between the bacterial endosymbionts and their host plants. Our results indicate a recent evolutionary process towards a stable and host specific interaction confirming the proposed maternal transmission mode of the endosymbionts through the seeds. Divergence estimates provide evidence for a relatively recent origin of bacterial leaf symbiosis, dating back to the Miocene (5–23 Mya). This geological epoch was characterized by cool and arid conditions, which may have triggered the origin of bacterial leaf symbiosis

Recent results on heavy-ion direct reactions of interest for 0νββ decay at INFN LNS

Neutrinoless double beta decay of nuclei, if observed, would have important implications on fundamental physics. In particular it would give access to the effective neutrino mass. In order to extract such information from 0νββ decay half-life measurements, the knowledge of the Nuclear Matrix Elements (NME) is of utmost importance. In this context the NUMEN and the NURE projects aim to extract information on the NME by measuring cross sections of Double Charge Exchange reactions in selected systems which are expected to spontaneously decay via 0νββ. In this work an overview of the experimental challenges that NUMEN is facing in order to perform the experiments with accelerated beams and the research and development activity for the planned upgrade of the INFN-LNS facilities is reported

The genome of the seagrass Zostera marina reveals angiosperm adaptation to the sea

Seagrasses colonized the sea(1) on at least three independent occasions to form the basis of one of the most productive and widespread coastal ecosystems on the planet(2). Here we report the genome of Zostera marina (L.), the first, to our knowledge, marine angiosperm to be fully sequenced. This reveals unique insights into the genomic losses and gains involved in achieving the structural and physiological adaptations required for its marine lifestyle, arguably the most severe habitat shift ever accomplished by flowering plants. Key angiosperm innovations that were lost include the entire repertoire of stomatal genes(3), genes involved in the synthesis of terpenoids and ethylene signalling, and genes for ultraviolet protection and phytochromes for far-red sensing. Seagrasses have also regained functions enabling them to adjust to full salinity. Their cell walls contain all of the polysaccharides typical of land plants, but also contain polyanionic, low-methylated pectins and sulfated galactans, a feature shared with the cell walls of all macroalgae(4) and that is important for ion homoeostasis, nutrient uptake and O-2/CO2 exchange through leaf epidermal cells. The Z. marina genome resource will markedly advance a wide range of functional ecological studies from adaptation of marine ecosystems under climate warming(5,6), to unravelling the mechanisms of osmoregulation under high salinities that may further inform our understanding of the evolution of salt tolerance in crop plants(7)

A focus on selected perspectives of the NUMEN project

The use of double charge exchange reactions is discussed in view of their application to extract information that may be helpful to determinate the nuclear matrix elements entering in the expression of neutrinoless double beta decay half-life. The strategy adopted in the experimental campaigns performed at INFN - Laboratori Nazionali del Sud and in the analysis methods within the NUMEN project is briefly described, emphasizing the advantages of the multi-channel approach to nuclear reaction data analysis. An overview on the research and development activities on the MAGNEX magnetic spectrometer is also given, with a focus on the chosen technological solutions for the focal plane detector which will guarantee the performances at high-rate conditions