Influência do tipo de lignina na caracterização de compósitos poliméricos com fibra natural

The use of recycled raw materials and renewable sources are necessary for economical, social, environmental and technological development. In this context, this work aims to study the influence of two lignin types, one derived from pine (Lig I) and the other one from eucalyptus, (Lig II) on polymer composites properties of recycled low density polyethylene (r-LDPE-Al) and Pinus Elliotti wood flour (WF), in the proportion 70% and 30% matrix/reinforcement in weight, respectively. The r-LDPE-Al is from Tetra Pak post-consumer packaging. The composites were processed by extrusion in a laboratory co-rotation twin-screw extruder. The composites obtained were evaluated through tests of melt flow index (MFI), tensile strength, Charpy impact strength, density and heat deflection temperature (HDT). The MFI results indicated that both lignin showed potential use as a flow agent for r-LDPE-AL/WF composites, with an increase of 41% and 13% for Lig I and Lig II, respectively, when compared with composite reference 0 (without lignin). Mechanical test results showed that the lignin origin influences the composites’ properties where Lig I, is derived from pine (the same source as wood flour) had the best performance, indicating a potential use as a coupling agent. The results were favorable for a more noble reuse for post-consumer packaging and the lignin by-product.O uso de matérias-primas recicladas e de fontes renováveis faz-se necessário tanto para o desenvolvimento econômico, social, ambiental quanto tecnológico. Nesse sentido, esse trabalho consiste no estudo da influência de 2 tipos de ligninas (uma derivada de pinus e denominada como Lig I e outra de eucalipto, denominada Lig II) na caracterização de compósitos poliméricos com polietileno de baixa densidade reciclado (r-PEBD-Al) e farinha de madeira (FM), da espécie Pinus Elliotti, na proporção 70% e 30% em massa de matriz/reforço, respectivamente. O r-PEBD-Al é oriundo das embalagens pós-consumo Longa vida. Os compósitos foram processados em extrusora de laboratório dupla rosca co-rotante. Os compósitos obtidos foram avaliados através dos ensaios de índice de fluidez (IF), tração, resistência ao impacto Charpy, densidade e temperatura de deflexão térmica (HDT). Os resultados de IF indicaram que ambas as ligninas apresentaram potencial uso como agente de fluxo para os compósitos de r-PEBD-Al/FM, com 41% e 13% de aumento para Lig I e Lig II, respectivamente, em relação ao compósito 0 sem lignina (referência). As propriedades mecânicas evidenciaram que a origem da lignina influencia no desempenho dos compósitos, sendo que a Lig I, que é derivada de pinus, mesma fonte que a farinha de madeira apresentou uso potencial como agente de acoplamento. Os resultados se mostraram favoráveis para um reaproveitamento mais nobre para as embalagens pós-consumo e o subproduto lignina

O impacto da esquizofrenia para a família

The impact of schizophrenia on the family has been compared to the trauma experienced by victims of catastrophes. Considering the social repercussion on the health system and the emotional repercussion on the schizophrenic individual, with consequences to the family group, an ethnographic case study was developed aimed at describing the impact of schizophrenia on the family. One family consisted of father, mother, and five children, four of whom are schizophrenic, participated in the study, and have had a follow-up in a public psychiatric service. Schizophrenia impacts family members through their suffering, isolation, and overloadEl impacto de la esquizofrenia en la familia ha sido comparado con el trauma vivido por las victimas de una catástrofe. Considerando las repercusiones sociales en el sistema de salud y el impacto emocional para el individuo esquizofrénico que incide en el grupo familiar, se desarrolló un estudio de tipo etnográfico cuyo objetivo fue describir el impacto de la esquizofrenia en la familia. Participó en este estudio un grupo familiar compuesto por padre, madre y cinco hijos, de los que cuatro son portadores de esquizofrenia y están en control en un servicio psiquiátrico comunitario. El impacto de la esquizofrenia en la familia fue revelado a través del sufrimiento, aislamiento y sobrecarga que imponeO impacto da esquizofrenia sobre a família tem sido comparado ao trauma vivido por vítimas de catástrofes. Considerando as repercussões sociais para o sistema de saúde e as de ordem emocional para o esquizofrênico, com desdobramento no grupo familiar, foi desenvolvido um estudo de caso etnográfico com objetivo de descrever o impacto da esquizofrenia na família. Participaram do estudo uma família composta por pai, mãe, cinco filhos, dos quais quatro são portadores de esquizofrenia e fazem seguimento num serviço psiquiátrico comunitário. O impacto da esquizofrenia para a família foi revelado pelo sofrimento, isolamento e sobrecarg

Generation of a human iPSC line from a patient with Leigh syndrome

Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513G. >. A; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (FPU13/00544). MEG is a staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER)

Generation of a human control iPSC line with a European mitochondrial haplogroup U background

Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to R.G); T.G. receives grant support from the Universidad Autónoma de Madrid, FPI-UAM and F.Z.D. from the Ministerio de Educación, Cultura y Deporte, grant number FPU13/00544. M.E.G. is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with a mitochondrial encephalopathy due to mutations in the GFM1 gene

Human iPSC line GFM1SV.25 was generated from fibroblasts of a child with a severe mitochondrial encephalopathy associated with mutations in the GFM1 gene, encoding the mitochondrial translation elongation factor G1. Reprogramming factors OCT3/4, SOX2, CMYC and KLF4 were delivered using a non integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional development fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (Grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (Grant FPU13/00544). MEG is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Citizenship against the Pelli-Cajasol skyscraper in Seville

Esta comunicación narra la historia de una derrota de la sociedad civil preocupada por su patrimonio cultural y sus paisajes históricos urbanos. Se trata de la trayectoria de la Plataforma Ciudadana ¡Túmbala! y del Manifiesto contra la Torre Pelli-Cajasol, organizaciones de asociaciones, colectivos, personas y profesionales de diferente perfil y procedencia que desde principios del año 2009 se oponen al rascacielos de 178 metros de altura que promueve Cajasol, ahora Caixabank, en el extremo sur de la Isla de la Cartuja de Sevilla, en la denominada Puerta Triana de acceso a la Exposición Universal de 1992. El edificio, en su fase final y a punto de inaugurarse, ha roto definitivamente el perfil histórico de Sevilla, imponiendo su presencia de forma hegemónica sobre la ciudad. El camino recorrido supone la lucha de la ciudadanía por participar de las decisiones que le afectan de forma directa, como pueden ser aquellas relativas a qué es y cómo se protege su patrimonio histórico y al simbolismo que contienen determinados espacios y paisajes urbanos, ya que conforman una parte del derecho a la ciudad de todos sus habitantes. Tal lucha, infructuosa habida cuenta del resultado, se detalla a lo largo del texto, que a su vez ilustra la brecha abierta entre las instituciones públicas en todas sus escalas y la sociedad civil, así como la dominación efectiva ejercida por el poder económico y financiero de la banca.This work tells the story of a defeat by the civil society that tries to protect its cultural heritage and its historic urban landscapes. It is about the trajectory of the Citizen Platform ¡Túmbala! (Knock it down!) and the Manifesto against the Pelli-Cajasol tower, which encompasses associations, social collectivities, people and professionals This work tells the story of a defeat by the civil society that tries to protect its cultural heritage and its historic urban landscapes. It is about the trajectory of the Citizen Platform ¡Túmbala! (Knock it down!) and the Manifesto against the Pelli-Cajasol tower, which encompasses associations, social collectivities, people and professionals.Depto. de Prehistoria, Historia Antigua y ArqueologíaFac. de Geografía e HistoriaTRUEMinisterio de Economía y Competitividad (MINECO)pu

Generation of a human iPSC line from a patient with Leigh syndrome caused by a mutation in the MT-ATP6 gene

Human iPSC line L749.1 was generated from fibroblasts of a patient with Leigh syndrome associated with a heteroplasmic mutation in the MT-ATP6 gene. Reprogramming factors OCT4, SOX2, CMYC and KLF4 were delivered using retroviruses.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” (FIS PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG cofunded by FEDER), “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to R.G); T.G-M. receives grant support from the Universidad Autónoma de Madrid, FPI-UAM and F.Z-D. from the Ministerio de Educación, Cultura y Deporte, grant number FPU13/00544. M.E.G. is senior staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with a defect of intergenomic communication

Human iPSC line PG64SV.2 was generated from fibroblasts of a patient with a defect of intergenomic communication. This patient harbored a homozygous mutation (c.2243G>C; p.Trp748Ser) in the gene encoding the catalytic subunit of the mitochondrial DNA polymerase gamma gene (POLG). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional development fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (Grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (Grant FPU13/00544). MEG is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with an optic atrophy ‘plus’ phenotype due to a mutation in the OPA1 gene

AbstractHuman iPSC line Oex2054SV.4 was generated from fibroblasts of a patient with an optic atrophy ‘plus’ phenotype associated with a heterozygous mutation in the OPA1 gene. Reprogramming factors OCT3/4, SOX2, CMYC and KLF4 were delivered using a non-integrative methodology that involves the use of Sendai virus

Avaliação da produção lacrimal em gambás filhotes por três diferentes métodos

O estabelecimento do parâmetro de produção lacrimal nas diferentes espécies é importante para o entendimento da saúde do olho e é um dos componentes da semiotécnica oftálmica. Particularidades derivadas da anatomofisiologia das espécies não domésticas induzem a busca de metodologias que sejam mais fidedignas aos parâmetros. Objetivou-se com este estudo avaliar e comparar a produção lacrimal de gambás-de-orelha-branca (Didelphis albiventris) e gambás-de-orelha-preta (Didelphis aurita) por três diferentes métodos. Quinze indivíduos de cada espécie, juvenis, hígidos, de ambos os sexos, com 60 a 90 dias de vida, foram contidos fisicamente para realização do teste lacrimal do vermelho de fenol (TLVF), da ponta de papel absorvente estéril e do teste lacrimal de Schirmer modificado (TLSm). O TLVF foi o mais difícil de ser executado devido à maleabilidade do fio, enquanto a TEPA se mostrou mais exequível para ambas as espécies. A mediana ± intervalo semi-interquartil para o TLVF foi de 19,79±2,61mm/15” e 5,22±2,92mm/15”, para a TEPA foram de 16,25±1,82mm/min e 10,93±3,04mm/min, e para o TLSm foram de 0±1,63mm/min e 0±1,63mm/min, para gambás-de-orelha-branca e gambás-de-orelha-preta, respectivamente. Não houve diferença entre o olho direito e esquerdo e nem quanto ao sexo. Obteve-se diferença significativa para um mesmo teste entre as espécies. Não foi encontrada correlação significativa entre os testes para ambas as espécies. A quantificação da porção aquosa da lágrima poderá auxiliar no diagnóstico precoce de doenças oculares nas espécies estudadas.The establishment of parameters for tear production in different species is important for better understanding eye´s health and is one of the components of the ophthalmic semiological technique. Particularities derived from the anatomophysiology of non-domestic species induce the search for more reliable methodologies. The aim was to evaluate and compare tear production of white-eared opossum (Didelphis albiventris) and Brazilian common opossum (Didelphis aurita) by three different methods. Fifteen individuals of each species, juveniles, healthy, of both sexes, with 60 to 90 days of life, were physically restrained. Phenol red thread test (PRTT), endodontic absorbent paper point tear test (EAPPTT) and modified -Schirmer tear test (mSTT) were performed. PRTT was the most difficult to perform because of the wire malleability, while EAPPTT was more feasible for both species. The median ± semi-quartile range for PRTT were 19.79±2.61mm/15 “and 5.22±2.92mm/15”, for EAPPTT were 16.25±1.82mm/min and 10.9±3.04mm/min, and for STTm were 0±1.63mm/min and 0±1.63mm/min for white-eared opossum and Brazilian common opossum respectively. There was no difference between the right and left eye neither sex. A significant difference was obtained for the same test to different species. No significant correlation was found between the tests for both species. The description of tear production parameters for juvenile white-eared opossum and Brazilian common opossum may be used as a tool, which will allow the early diagnosis of ocular diseases